18-1114-21

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Ion Exchange Chromatography Principles and Methods

Ion Exchange Chrom atography – Principles and M

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Ion Exchange Chromatography

Principles and Methods

ISBN 91 970490-3-4

1. Introduction ............................................................................................9

2. Ion exchange chromatography .........................................................10 The theory of ion exchange.................................................................10

The matrix.....................................................................................11 Charged groups .............................................................................13 Resolution in ion exchange chromatography .................................13

Capacity factor ........................................................................15 Efficiency.................................................................................16 Selectivity.................................................................................17

Capacity ........................................................................................18

3. Product Guide .......................................................................................20 MonoBeads ...................................................................................20 MiniBeads .....................................................................................20 SOURCE .......................................................................................20 Sepharose High Performance ion exchangers .................................21 Sepharose Fast Flow ion exchangers ..............................................21 Sepharose Big Beads ion exchangers...............................................21 STREAMLINE ion exchangers ......................................................21 DEAE Sepharose CL-6B and CM Sepharose CL-6B .......................22 DEAE Sephacel..............................................................................22 Sephadex ion exchangers ...............................................................22 Bulk quantities...............................................................................22 Equipment.....................................................................................22

4. MonoBeads and MiniBeads ..............................................................23 MonoBeads.....................................................................................23

Properties ......................................................................................24 Chemical stability ....................................................................24 Physical stability ......................................................................25 Flow rate..................................................................................27 Capacity ..................................................................................27 Recovery..................................................................................28 Reproducibility ........................................................................29

Availability ....................................................................................30 MiniBeads .......................................................................................30

Properties ......................................................................................31 Chemical stability ....................................................................31 Physical stability ......................................................................32 Reproducibility ........................................................................33

Availability ....................................................................................33

Contents

2

5. SOURCE ................................................................................................34 Properties ......................................................................................37

Chemical stability ....................................................................37 Flow rate..................................................................................38 Capacity ..................................................................................40 Recovery..................................................................................40 Reproducibility ........................................................................41

Availability ....................................................................................41

6. Sepharose based ion exchangers .......................................................42 Chemical stability ..........................................................................42 Physical stability ............................................................................43

Sepharose High Performance ion exchangers.........................43 Properties ......................................................................................44

Physical stability ......................................................................44 Capacity ..................................................................................44 Flow rate..................................................................................46

Availability ....................................................................................46 Sepharose Fast Flow ion exchangers ........................................46

Properties ......................................................................................47 Physical stability ......................................................................47 Capacity ..................................................................................47 Flow rate..................................................................................49

Availability ....................................................................................50 Sepharose Big Beads ion exchangers.........................................50

Properties ......................................................................................51 Physical properties ...................................................................51 Capacity ..................................................................................51 Flow rate..................................................................................51

Availability ....................................................................................51 STREAMLINE SP and STREAMLINE DEAE ......................52

Properties ......................................................................................53 Physical stability ......................................................................53 Capacity ..................................................................................53

Availability ....................................................................................54 DEAE Sepharose CL-6B and CM Sepharose CL-6B.............54

Properties .....................