Investigating and Modeling Natural Biodegradation System in Soil Mythreyi Chandoor, Deepak Singh and...
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![Page 1: Investigating and Modeling Natural Biodegradation System in Soil Mythreyi Chandoor, Deepak Singh and Shulin Chen Bioprocessing and Bioproduct Engineering.](https://reader036.fdocuments.in/reader036/viewer/2022062423/5697c00b1a28abf838cc8367/html5/thumbnails/1.jpg)
Investigating and Modeling Natural Investigating and Modeling Natural Biodegradation System in Soil Biodegradation System in Soil
Mythreyi Chandoor, Deepak Singh and Shulin Chen
Bioprocessing and Bioproduct Engineering Laboratory, Department of Biological
Systems Engineering Washington State University .
![Page 2: Investigating and Modeling Natural Biodegradation System in Soil Mythreyi Chandoor, Deepak Singh and Shulin Chen Bioprocessing and Bioproduct Engineering.](https://reader036.fdocuments.in/reader036/viewer/2022062423/5697c00b1a28abf838cc8367/html5/thumbnails/2.jpg)
Outline
•Aim and importance of the project• Background – Hypothesis of the project• Experimental:
MicrobiologyChemical analysis of lignocellulose degradation in soil.
• ModelingLignocellulose degradation in soilApplication
• Acknowledgements
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Aim and importance of the project
•Demand for an Alternative Fuel The U.S. ethanol consumption is forecast to increase from 5.6 billion gallons last year to 13.5 billion gallons in 2012, (Thomson Reuters, 2009).
•What are the challenges ? One of the challenges lies in the deconstruction of lignin part of the biomass to release sugars.
Need for novel pretreatment technology !
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Delignification, repolymerization
Humus formation
Proteins
Hypothesis: Lignocellulosic degradation system
in soil
Parallel degradation of cellulose and hemicellulose
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Lignin –Major Linkages in the structure
OH
HO
HO
OOCH3- O- 4
4
o OH
OH
OH
4
-O-4
OH
OH
HO
HO
-
O
OH
5
5
5-5
O4-O-5
OR
-Hydroxyphenol
Lignin
OR
H3CO OCH3
Syringyl
Lignin
OR
OCH3
Guaiacyl
Lignin
O
OCH3O
R
OO
O
OXyl Xyl
HO
OO
HOO
O
O
O--H3CO OCH3
O
OXyl
HOOH
OHOOCH3
H3CO
o
O
HO
OCH3
OH
O
O
CH
CH
C O
O CH
CH2OH
COH
O CH
CH2OH
O
H3CO OCH3
HC
CH2OH
H2C
OOCH3
CH
CH2OH
HOHC
O
H2C
CH2OH
H2C
OH
H2C
CH2OH
CH
H3CO
R
OCH3
H3CO
O
O
OHHO
H3CO
H3CO
O
O
H3CO
OH
OH
OOH
HO O
HO
Lignin surrounds cellulose microfilbrils ,there by providing strength to the cell wall. Highly
heterogeneous material ,requires Oxygen for its degradation /modification .
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•To understand the biodegradation of lignocellulose in soil
• To model the biodegradation of lignocellulose in soil
•To understand the biodegradation of lignocellulose in soil
• To model the biodegradation of lignocellulose in soil
Design the pretreatment system
Aim of the Project
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Methodology
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Scanning Electron Microscopy (SEM)
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C2,C3,C5 of cellulose
C4 of amorphous and crystalline
cellulose
4 weeks 8 weeks
12 weeks16 weeks
Solid State NMR Analysis
Syringol
Guiacyl
Sinapyl,coniferyl
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Batch samples for every four weeks
% C
once
ntr
atio
n o
f th
e to
tal
com
pou
nd
Pyrolysis-GC/MS Analysis
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Thermogravitometric Analysis
temperature
0 5 10 15 200 250 300 350 400 450 500 550
Soil Sample S5
Soil sample S4,
After 20 weeks
After 16 weeks
After 12 weeks
After 8 weeks
After 4 weeks
Cellulose
Lignin
Hemicellulose
40
60
80
100
20
Weig
ht
perc
en
t (%
)
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[S]+[X]+O2 + H2Osoil
pH
[p] +[s1] + co2 +[X]
Input = output +accumulation – generation
where in S = s1+ s2 + s3
.
X = x1 +x2 +x3 .
P = products ( glucose and other residual sugars ).S1 = modif ied lignin .
( s1 =cellulose , s2= hemicellulose, s3= lignin )
(Maximum microbial growth on the biomassrespectively )
General Equation for the Soil Degradation system
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d(S1) / d(t) = -Vb1*S1*X1/(Ks1+S1)S1(0) = 0.71 # weight in gm/gm #Cellulose Balance
d(S2) / d(t) = -Vb2*S2*X2/(Ks2+S2)S2(0) = 0.48 # weight in gm/gm #Hemicellulose Balance
d(S3) / d(t) = -Vb3*S3*X3/(Ks3+S3)S3(0) = 0.28 # weight in gm/gm #Lignin Balance
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#Microbial Growth corresponding to Cellulosed(X1) / d(t) = μ1*X1 X1(0) = 0.1 #Microbial Growth corresponding to Hemicellulosed(X2) / d(t) = μ 2*X2
X2(0) = 0.1 #Microbial Growth corresponding to Lignind(X3) / d(t) = μ3*X3
X3(0) = 0.1 µ1=µmax1*S1/(Ks1+S1)-∆1
µ2=µmax2*S2/(Ks2+S2)-∆2 µ3=µmax3*S3/(KS3+S3)-∆3
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t(0) = 0t(f) = 3360
Considering the values as follows ;µmax1=0.08µmax2=0.05μmax3=0.03 system. ∆1=0.001∆2=0.001∆3=0.001
We derived an relation using polymath which defines the degradation pattern in the soil
Initial and final time periods of Incubation
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Time (in hours )
Init
ial S
ubst
rate
co n
cent
r ati
on in
gm
/ gm
We derived an relation using polymath which defines the degradation pattern in the soil system.
Modeling
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Application of the Model
The determination of the exact relation between the various factors with respect to the specific incubation period would be helpful in developing a model which would predict the specific ratio of cellulose, hemicellulose and lignin apart from other factors involved such as pH, temperature and other organic compounds.
Thus providing a suitable mechanism for the pretreatment technology !
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Conclusion
Scientific Contribution:
Specific conditions which relates to the changes in the relative composition of cellulose,hemicellulose and lignin, Chemical changes in the lignin structure with respect to time as a result of which lignin modification in the soil takes place during the natural degradation process was determined
Implication: As the pathway of lignin degradation in soil is quite different this would provide us a scope for a novel method for lignin degradation process. Further work would involve understanding different factors which play an important role during the lignin modification and degradation .
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I would like to thank
•Dr. Ann Kennedy USDA-ARS Soil Scientist/Adj. Prof. Crop and Soil Sciences,WSU.
•Dr. Greg Helms, NMR Center Director ,WSU.•Dr. Manuel Garcia-Perez. Assistant Professor / Scientist. Biological Systems Engineering ,WSU.•Dr. Bill , Assistant manager ,NMR Center,WSU.
And my Advisor …•Dr. Shulin Chen, Professor/Scientist. Department of Biological Systems Engineering,WSU .
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My Team …
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Thank you …
Any Questions ?