Introduction to the compound Microscope. Types of Microscopes Compound Microscope Stereoscope or...
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Transcript of Introduction to the compound Microscope. Types of Microscopes Compound Microscope Stereoscope or...
![Page 1: Introduction to the compound Microscope. Types of Microscopes Compound Microscope Stereoscope or dissecting scope Onion cells (100x) Cheek cells (400x)](https://reader033.fdocuments.in/reader033/viewer/2022061616/56649f045503460f94c187ba/html5/thumbnails/1.jpg)
Introduction to the compound Microscope
![Page 2: Introduction to the compound Microscope. Types of Microscopes Compound Microscope Stereoscope or dissecting scope Onion cells (100x) Cheek cells (400x)](https://reader033.fdocuments.in/reader033/viewer/2022061616/56649f045503460f94c187ba/html5/thumbnails/2.jpg)
Types of Microscopes
Compound Microscope
Stereoscope or dissecting scope
Onion cells (100x) Cheek cells (400x)
Two spotted spider mite (20x)
![Page 3: Introduction to the compound Microscope. Types of Microscopes Compound Microscope Stereoscope or dissecting scope Onion cells (100x) Cheek cells (400x)](https://reader033.fdocuments.in/reader033/viewer/2022061616/56649f045503460f94c187ba/html5/thumbnails/3.jpg)
Types of Microscopes
TEM - Transmission Electron Microscope
![Page 4: Introduction to the compound Microscope. Types of Microscopes Compound Microscope Stereoscope or dissecting scope Onion cells (100x) Cheek cells (400x)](https://reader033.fdocuments.in/reader033/viewer/2022061616/56649f045503460f94c187ba/html5/thumbnails/4.jpg)
Types of Microscopes
SEM - Scanning Electron Microscope
![Page 5: Introduction to the compound Microscope. Types of Microscopes Compound Microscope Stereoscope or dissecting scope Onion cells (100x) Cheek cells (400x)](https://reader033.fdocuments.in/reader033/viewer/2022061616/56649f045503460f94c187ba/html5/thumbnails/5.jpg)
Eye piece
Body Tube
Revolving nosepiece
Objectives
Stage Clips
Stage
Diaphragm
Mirror (light source) Base
Coarse Adjustment
Fine Adjustment
Arm
![Page 6: Introduction to the compound Microscope. Types of Microscopes Compound Microscope Stereoscope or dissecting scope Onion cells (100x) Cheek cells (400x)](https://reader033.fdocuments.in/reader033/viewer/2022061616/56649f045503460f94c187ba/html5/thumbnails/6.jpg)
• Always carry with 2 hands. Place one hand on the arm and one hand on the base.
• Make sure the microscope is on a flat surface and away from the edge of the table.
• Always Look to the side when changing objectives and rotate carefully.
• Always use low (scanning) power first.• Always store the microscope covered and with the
lowest powered objective in place.• Only use lens paper for cleaning
Rules for using the microscope
![Page 7: Introduction to the compound Microscope. Types of Microscopes Compound Microscope Stereoscope or dissecting scope Onion cells (100x) Cheek cells (400x)](https://reader033.fdocuments.in/reader033/viewer/2022061616/56649f045503460f94c187ba/html5/thumbnails/7.jpg)
Microscope Basics: Determining Total Magnification
• Multiply the magnification of the ocular lens (eyepiece) by the magnification of the objective lens.
10 x
4 x
Total magnification = 10 x 4 = 40
We represent this magnification as 40X
![Page 8: Introduction to the compound Microscope. Types of Microscopes Compound Microscope Stereoscope or dissecting scope Onion cells (100x) Cheek cells (400x)](https://reader033.fdocuments.in/reader033/viewer/2022061616/56649f045503460f94c187ba/html5/thumbnails/8.jpg)
Microscope Basics: Field of view
• Field of view is what you see when you look into a microscope.
As you increase magnification your field of view becomes smaller
![Page 9: Introduction to the compound Microscope. Types of Microscopes Compound Microscope Stereoscope or dissecting scope Onion cells (100x) Cheek cells (400x)](https://reader033.fdocuments.in/reader033/viewer/2022061616/56649f045503460f94c187ba/html5/thumbnails/9.jpg)
![Page 10: Introduction to the compound Microscope. Types of Microscopes Compound Microscope Stereoscope or dissecting scope Onion cells (100x) Cheek cells (400x)](https://reader033.fdocuments.in/reader033/viewer/2022061616/56649f045503460f94c187ba/html5/thumbnails/10.jpg)
Microscope Basics: Depth of focus.
• Refers to how much of the specimen you can focus clearly on at one time in your field of view.
• As you increase magnification your depth of focus becomes narrower and less of your sample can be focused at one time.