Introduction to Neurobiology Lecture 13: Classical conditioning 1.
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Transcript of Introduction to Neurobiology Lecture 13: Classical conditioning 1.
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Introduction to NeurobiologyLecture 13: Classical conditioning 1
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Associative
Learning
Nonassociative
Habituation Sensitization
A single type of stimulus
The relationship between two stimuli or reward .
Classical
conditioning Operant
conditioning Extinction
Blocking
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Definitions
An Unconditioned stimulus (US) is a stimulus which “naturally” elicits an (unconditioned) response (UR). For example:An airpuff to the eye is an unconditioned stimulus which elicits an eyeblink (nictitating membrane response).
A conditioned stimulus (CS) is a “neutral” stimulus (e.g. a tone), which, by being associated with the US, leads after learning to a conditioned response (CR).
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Basic paradigm
Before learning,US URCS nothing
Training:CS+US URCS increasing CR(=UR)
Post-training:CS CR (=UR).Temporal relationship :
1 )Simultaneous 2 )Delayed (partial overlap in time)
3 )Trace (separated in time. Usually 200ms-2s.
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The nictitating membrane response
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Circuitry for conditioning nictitating membrane response
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The suppression of CF during learning of eye blink.
Naive animal
Trained animal
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US only
CS-US
With PTX
GABA is involved in the suppression of CF.
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Extinction protocol with :no infusion
ACSF PTX
GABA is involved in the extinction of CR.
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The response of trained animal to tone + puff
test sessions with: ACSF
NBQX (AMPA receptor antagonist)
AMPA receptors are involved in the extinction of CR.
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Blocking: CS “A” is tone; CS “B” is light; US is air puff.The eyeblink response to air puff is the UR The Eyeblink response to A or B is the CR
B+US C
RB
A+B+US C
RA/BA+US
A+B+USCR
ACR
B //
CR
AA+US
Blocking of conditioned eyelid responses
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Phase I, animals received seven daily sessions of tone-airpuff conditioning ;Phase II, animals underwent five sessions of tone-light-airpuff compound conditioning.Either picrotoxin )PTX) or )ACSF) was infused directly into the inferior olive.Controls experienced only the second phase of the blocking procedure .
Afterward, all animals were presented with light-airpuff pairings to assess whether conditioning to the light had accrued during compound conditioning )phase II).
Both control and PTX animals exhibited significant learning to the light CS compared with the ACSF animals - blocking did not occur in the PTX group.
PTX had no effect on the performance of CRs and URs during the compound conditioning, indicating that PTX selectively affected blocking.
ACSF
PTX
CONTROL
Phase 1 phase 2 phase 3
Blocking
GABA is involved in blocking of conditioned eyelid responses
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Circuitry for conditioning nictitating membrane response
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Wild-type:
Conditioned responses (%)- 80
Average peak amplitude- 0.68
Average peak velocity- 32.6 mm/s
LTD-deficient mice:
Conditioned responses (%)- 30
Average peak amplitude- 0.44
Average peak velocity- 19.6 mm/s
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Average CS-alone responses of a wild-type animal and a L7-PKCi mutant
At T-4 (but not at T-2) the wt shows well-timed responses around the US onset time.
The response peak of the mutant doesn’t change.
At T-4 (but not at T-2) there are changes between wt and the mutant.
Blue- wild typeRed- mutants
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Average CS-alone responses when the ISI is extended to 500 ms
If the timing is LTD-dependent, the amplitude and velocity in the L7-PKCi mutant should not be influenced by the length of the ISI.
indeed, the mutants’ peak amplitude and peak velocity weren’t changed.
In contrast, those wt values were changed.
Blue- wild typeRed- mutants
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Red - pre-lesion
Blue – post-lesion
Green - post-sham lesion
Mutants wt
)After T-4)
Conditioned responses still occur after lesions of the cerebellum in both mutants and wt, but the amplitudes were significantly reduced.
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Blocking neuronal activity in cerebellar output
Superior cerebellar peduncle blocked using perfusion of TTX
Expression of eyeblink conditioning blocked, but not acquisition
Krupa and Thompson, PNAS 1995
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The cerebellar-olivary system
LTD
LTP
1.2Hz<>Rate
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phase 1 : seven daily sessions of tone-airpuff conditioning.
phase 2 : five sessions of tone-light-airpuff compound conditioning while either PTX or ACSF was infused.
phase 3 : all animals were presented with light-airpuff pairing tests.
controls experienced only the second phase of the blocking procedure.
Blocking of conditioned eyelid responses
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olivepons
PF CF
PC’s
LTD
DCN
CR
Blocking: US inhibition
US CS
PTX: blocks inhibition
prevents blocking
prevents extinction
Extinction: without US or with NBQX
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The daily training consisted of 10 blocks of 10 trials.
The trials were separated by a random intervals )20 to 40 ms).
Response of wild-type )wt) after 2 days and after 4 days.CR- conditioned response
UR- unconditioned response