Introduction to Bioinformatics Lecturer: Prof. Yael Mandel-Gutfreund Teaching Assistance: Rachelly Normand Olga Karinski Course web site : 2 What is Bioinformatics? 3 Course Objectives To introduce the bioinfomatics discipline To make the students familiar with the major biological questions which can be addressed by bioinformatics tools To introduce the major tools used for sequence and structure analysis and explain in general how they work (limitation etc..) 4 Course Structure and Requirements 1.Class Structure 1.2 hours lecture 2.1 hour tutorial 2. Home work Homework assignments will be given every second week The homework will be done in pairs. 4/4 homework assignments will be submitted 2. A final project will be conducted in pairs * Project will be presented as a poster poster day 9.3 5 Grading 20 % Homework assignments 80 % final project (10% proposal, 20% supervisor evaluation 70% poster presentation) 6 What is Bioinformatics? 7 The field of science in which biology, computer science, and information technology merge to form a single discipline Ultimate goal: to enable the discovery of new biological insights as well as to create a global perspective from which unifying principles in biology can be discerned. What is Bioinformatics? 8 Central Paradigm in Molecular Biology mRNAGene (DNA)Protein 21 ST centaury GenomeTranscriptomeProteome 9 From DNA to Genome Watson and Crick DNA model First human genome draft First genome Hemophilus Influenzae Yeast genome Total complete genomes = 21,636 11 Main Goal: To understand the living cell AnnotationComparative genomics Functional genomics genomes Whats Next ? The post-genomics era Systems Biology And beyond Personalized medicine 12 From genomes ToUnderstanding living cells 14 CCTGACAAATTCGACGTGCGGCATTGCATGCAGACGTGCATG CGTGCAAATAATCAATGTGGACTTTTCTGCGATTATGGAAGAA CTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTC AGAATGCTTTTAATAAGCGGGGTTACCGGTTTGGTTAGCGAGA AGAGCCAGTAAAAGACGCAGTGACGGAGATGTCTGATG CAA TAT GGA CAA TTG GTT TCT TCT CTG AAT TGAAAAACGTA Annotation 15 Annotation Identify the genes within a given sequence of DNA Identify the sites Which regulate the gene Predict the function What do they do??? 16 How do we identify a new gene in a genome? A gene is characterized by several features (promoter, ORF) some are easier and some harder to detect 17 Using Bioinformatics approaches for Gene hunting Relative easy in simple organisms (e.g. bacteria) Still VERY HARD for higher organism (e.g. humans) 18 Comparative genomics 19 Comparison between the full drafts of the human and chimp genomes revealed that they differ only by 1.23% How can we be so similar--and yet so different? How humans are chimps? Perhaps not surprising!!! 20 Where are we different ?? Where are we similar ??? VERY SIMAILAR Conserved between many organisms VERY DIFFERENT 21 Human ATAGCGGGGGGATGCGGGCCCTATACCC Chimp ATAGGGG--GGATGCGGGCCCTATACCC Mouse ATAGCG---GGATGCGGCGC-TATACC-A Human ATAGCGGGGGGATGCGGGCCCTATACCC Chimp ATAGGGGGGATGCGGGCCCTATACCC Mouse ATAGCGGGATGCGGCGCTATACCA Sometime minor changes in critical genes can make a big difference 22 Single change in a genes among humans can be responsible for sever diseases Sickle Cell Anemia Due to 1 swapping of an A for a T Image source: 23 Healthy Individual >gi| |ref|NM_ | Homo sapiens hemoglobin, beta (HBB), mRNA ACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGA GG A GAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGC AGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATG CTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGC TCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGAT CCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCA CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCA CTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACT GGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGC >gi| |ref|NP_ | beta globin [Homo sapiens] MVHLTP E EKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLG AFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVAN ALAHKYH 24 Diseased Individual >gi| |ref|NM_ | Homo sapiens hemoglobin, beta (HBB), mRNA ACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGA GG T GAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGC AGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATG CTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGC TCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGAT CCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCA CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCA CTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACT GGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGC >gi| |ref|NP_ | beta globin [Homo sapiens] MVHLTP V EKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLG AFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVAN ALAHKYH 25 Functional genomics 26 TO BE IS NOT ENOUGH Expressed Not Expressed ( )( ) The difference in the brain size between Human and apes is mainly related to the different levels of the genes expression and not their content 28 Systems Biology Jeong et al. Nature 411, (2001) Biological networks What can we learn from Biological Networks Is the protein essential for the organism ? Is it a good drug targets? What can we learn about this protein How can bioinformatics contribute to Medicine?31 What of all this will we learn in the course? 32 The course will concentrate on the bioinformatics tools and databases which are used to : - Annotate genes, - Compare genes and genomes - Infer the function of the genes and proteins - Analyze the interactions between genes and proteins ETC. 33 1. Introduction to bioinformatics 2. Pairwise alignment 3. Database search 4. Protein alignments 5. MSA and phylogenetic analysis 6. Sequencing 7. Motif search-function prediction 8. Gene expression 9. Structural bioinformatics (proteins and RNA) 10. Biological networks What of all this will we learn in the course?