Introduction: Fats (lipid) : consist of a wide group of compounds that are generally soluble in...
8
EXP. ,,6,, ENZYMATIC DIGESTION OF FAT BY PANCREATIC LIPASE Introduction: Fats (lipid) : consist of a wide group of compounds that are generally soluble in organic solvents and largely insoluble in water. Chemically , fats are generally triesters of glycerol and fatty acids
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Transcript of Introduction: Fats (lipid) : consist of a wide group of compounds that are generally soluble in...
EXP. ,,6,,ENZYMATIC DIGESTION OF FAT BY PANCREATIC
LIPASEIntroduction:
Fats (lipid) : consist of a wide group of compounds that are generally soluble in organic solvents and largely insoluble in water. Chemically, fats are generally triesters of glycerol and fatty acids
A triglyceride consists of a glycerol and three fatty acids. It is the major type of lipid used for energy storage...
Fatty Acids: The lipid building blocks.
Lipases: are enzymes which hydrolyse the triglycerides, releasing fatty acids.
+3H₂O lipase glycerol + f.a
Triglycerides structure:
The hydrolysis process can give all three fatty acids or it can yield one or two F.AS with diglycerides and monoglycerides...
FA1 FA2
FA3 FA2 Lipase + Lipase FA FA1
FA3 FA2
Materials:
1. Fresh whole milk
2. Litmus solution ( or litmus paper) as an indicator.
3. Pancreatin solution (boiled, unboiled).
4. 1% Calcium hydroxide solution.
Procedure:
4ml milk half of A+
+ 10 drops 1ml boiled
Litmus + 3 drops pancreatin so
Ca(OH) 2
+ 1ml unboiled
in water bath (45⁰c) for4-6 hours.
Results:Accumulation of free fatty
acids will reduce the PH.Pink colour with indicatorOdourPrecipitate.
