Introduction: Cloud, Linux and basic skills

Mick WatsonDirector of ARK-Genomics

The Roslin Institute

Structure• Setting up a new Cloud instance• Accessing Linux via Putty• Introduction to Linux command-line• Basic manipulations• Quality control of Illumina data• Trimming of Illumina data

Why are you here?

What do you want to do?

This is going to be hard

How much do you want to succeed?

This can change your life / career

This could make you very employable

You can’t learn this in a week

You will need to devote time, and work hard

Some of you will do this

Many of you won’t

Who wants to succeed?

Recommendation• Buy a laptop (lots of RAM, 64bit)• Install Ubuntu, or BioLinux• Force yourself to work on the command-line• Get on your institute’s linux servers• Get on your institute’s linux clusters• Use Google!• Set yourself a task and work at it. Do it yourself. Use Google.

Only ask for help when really stuck• Work at it – the rewards are huge• These skills are in huge demand -> jobs, pay rises, promotion,

papers etc etc

The course• Take your time• Read the materials• Understand the materials• Complete the exercises• Think about what is happening

Don’t panic!

mick.watson@roslin.ed.ac.uk

@BioMickWatson