Introduction

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V From time to time, an area of scientific endeavour will become such a focal point of attention that it warrants special treatment in the literature. Trends Guides are a unique service to our readers aimed at addressing these emerging information needs. Trends Guides are supplementary publications produced in association with one or more Trends journals. Each guide focuses on a topical subject area, often touching on fields that are not covered extensively or exclusively by any one Trends journal. Proteomics is one such topic that has moved rapidly to the forefront of scientific thinking, with interest spanning traditional life science boundaries. We are indebted to our Guest Editors Walter Blackstock and Matthias Mann, and to all the authors who have worked extremely hard over recent months to ensure that Proteomics: A Trends Guide is as informative and timely as possible. Proteomics: A Trends Guide is being circulated with the August issues of Trends in Biotechnology, Trends in Biochemical Sciences, Trends in Genetics, Trends in Microbiology and Drug Discovery Today. We hope that you enjoy reading this supplement and welcome your feedback and ideas. Rachel Wood Managing Editor [email protected] 1 Editorial – Proteomics comes of age: the quest for function by Walter Blackstock and Matthias Mann 3 Advances in 2D gel techniques by Angelika Görg 7 Experimentalism in proteomics by Jeffrey D. Thomas and Ruth A. VanBogelen 12 Trends in automation and mass spectrometry for proteomics by Walter Blackstock 17 Databases and knowledge resources for proteomics research by Matthew E. Crawford, Michael E. Cusick and James I. Garrels 22 Database searching with mass-spectrometric information by Ronald C. Beavis and David Fenyö 27 New methods of proteome analysis: multidimensional chromatography and mass spectrometry by Michael P. Washburn and John R. Yates, III 31 Using mass spectrometry for quantitative proteomics by Steven P. Gygi and Ruedi Aebersold 36 Modification-specific proteomics: systematic strategies for analysing post-translationally modified proteins by Ole Nørregaard Jensen 43 Protein-interaction mapping for functional proteomics by Ole Vorm, Angus King, Keiryn L. Bennett, Thomas Leber and Matthias Mann 47 Protein arrays: a high-throughput solution for proteomics research? by Dolores J. Cahill XII Company profile Oxford Glycosciences XIV Company profile Proteome Systems Cover image The cover image depicts a multi-function tool from a proteomics workstation built to the specifications of the Cell Map Project, Glaxo Wellcome, by KayeBee Engineering, Basildon. The unit incorporates imaging, gel-cutting, bar code reading and nanolitre volume liquid dispensing, and cuts at up to 1020 gel cores per hour. Editors Walter Blackstock and Matthias Mann Assistant Editor John Pettigrew Editorial Assistant Joanna Milburn Production Mick Ayres Managing Editor Rachel Wood Publishing Manager Rob Brines Contents Proteomics: A Trends Guide

Transcript of Introduction

Page 1: Introduction

V

From time to time, an area of scientificendeavour will become such a focalpoint of attention that it warrantsspecial treatment in the literature.Trends Guides are a unique serviceto our readers aimed at addressingthese emerging information needs.

Trends Guides are supplementarypublications produced in associationwith one or more Trends journals. Eachguide focuses on a topical subjectarea, often touching on fields that arenot covered extensively or exclusivelyby any one Trends journal.

Proteomics is one such topic thathas moved rapidly to the forefront ofscientific thinking, with interestspanning traditional life scienceboundaries. We are indebted to ourGuest Editors Walter Blackstockand Matthias Mann, and to all theauthors who have worked extremelyhard over recent months to ensure thatProteomics: A Trends Guide is asinformative and timely as possible.

Proteomics: A Trends Guide isbeing circulated with the August issuesof Trends in Biotechnology, Trends inBiochemical Sciences, Trends inGenetics, Trends in Microbiology andDrug Discovery Today.

We hope that you enjoy reading thissupplement and welcome yourfeedback and ideas.

Rachel WoodManaging [email protected]

1 Editorial – Proteomics comes of age: the quest for functionby Walter Blackstock and Matthias Mann

3 Advances in 2D gel techniquesby Angelika Görg

7 Experimentalism in proteomicsby Jeffrey D. Thomas and Ruth A. VanBogelen

12 Trends in automation and mass spectrometry for proteomicsby Walter Blackstock

17 Databases and knowledge resources for proteomics researchby Matthew E. Crawford, Michael E. Cusick and James I. Garrels

22 Database searching with mass-spectrometric informationby Ronald C. Beavis and David Fenyö

27 New methods of proteome analysis: multidimensional chromatography and mass spectrometryby Michael P. Washburn and John R. Yates, III

31 Using mass spectrometry for quantitative proteomicsby Steven P. Gygi and Ruedi Aebersold

36 Modification-specific proteomics: systematic strategies foranalysing post-translationally modified proteinsby Ole Nørregaard Jensen

43 Protein-interaction mapping for functional proteomicsby Ole Vorm, Angus King, Keiryn L. Bennett, Thomas Leber and Matthias Mann

47 Protein arrays: a high-throughput solution for proteomics research?by Dolores J. Cahill

XII Company profileOxford Glycosciences

XIV Company profileProteome Systems

Cover imageThe cover image depicts a multi-function tool from aproteomics workstation built to the specifications of theCell Map Project, Glaxo Wellcome, by KayeBeeEngineering, Basildon. The unit incorporates imaging,gel-cutting, bar code reading and nanolitre volume liquiddispensing, and cuts at up to 1020 gel cores per hour.

Editors Walter Blackstock and Matthias Mann Assistant Editor John Pettigrew Editorial Assistant Joanna Milburn Production Mick Ayres Managing Editor Rachel WoodPublishing Manager Rob Brines

Contents

Proteomics: A Trends Guide

-PROTEOMICS supp. [final] 14/7/00 11:59 am Page V

 · Contents INTRODUCTION Introduction

 · Contents INTRODUCTION Introduction

Introduction Introduction (ctnd.)

Introduction Introduction (ctnd.)

Introduction Section I: Introduction

Introduction Section I: Introduction

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