Introducing The PHERAstar FSX - ELRIG
Transcript of Introducing The PHERAstar FSX - ELRIG
Introducing The PHERAstar FSX
The NEW Gold Standard for HTS
Catherine Wark ELRIG snapshot April 2016
FSX - Introducing the key features
• The PHERAstar FSX builds on the success of the PHERAstar FS launched in 2009
• All PHERAstar FS features are included in the FSX
• Increased Sensitivity in FI, TRF, TR-FRET, AS, FP & Lumi
• Faster read times in TR-FRET with new faster laser
• Broader Dynamic range in Luminescence (9 decades)
• Improved Alpha Technology performance
• New Applications –Introducing Alphaplex technology with Simultaneous Dual Emmision
Better Sensitivity FI and FP
Plate Format PHERAstar FS PHERAstar FSX Conditions
384 0.4 pM Fluorescein 0.15 pM Fluorescein 20 µL - 384 small volume
1536 2.0 pM Fluorescein 0.5 pM Fluorescein 10 µL - 1536 HiBase
Plate Format PHERAstar FS PHERAstar FSX Conditions
3841.0 mP SD at 1 nM
Fluorescein0.5 mP SD at 1 nM
Fluorescein20 µL - 384 small volume,
15363.0 mP SD at 1 nM
Fluorescein1.5 mP SD at 1 nM
Fluorescein10 µL - 1536 HiBase
Better TRF/TR-FRET Performance
Light Source PHERAstar FS PHERAstar FSX
Xenon Flashlamp 300 flashes / sec 300 flashes / sec
TRF Laser 40 flashes / sec 60 flashes / sec
The energy per Laser flash is the same for PHERAstar FS and PHERAstar FSX
Added Performance50 % more Laser flashes within the same read time per well
50 % higher counts for donor and acceptor
better statistics, higher Z‘Enhanced Speed33 % shorter read time per well at the same number of Laser flashes
speed advantage
shortest 1536 read time with TRF Laser: 36 sec (flying mode, 1 flash)
PHERAstar FSX Lumi & Alpha Performance
PHERAstar FS PHERAstar FSX
Luminescence Dynamic Range 2,000,000 Counts / sec 10,000,000 Counts / sec
Orders Dynamic range 8 Orders 9 Orders
• Simultaneous Dual Emission Detection for Alpha Technology Using AlphaPlex beads
• Improvement to AlphaScreen Sensitivity
• Improvements signal to noise
Luminescence
Alpha Technology
Lumi and Alpha Cross talk reduction
Standard white plate Alpha plate
Advanced Cross Talk reduction wizards built in to avoid Cross talk through the walls of the plate
Summary of improvements
- Significant sensitivity improvements in FI and FP- General Improved HTRF performance with Flash lamp- TRF Nitrogen Laser with 60Hz repetition rate: faster reading- 5 times higher count rate than FS (10 Mio counts/sec):
extended dynamic range in LUM and AlphaTechnologies - Improved crosstalk reduction functionality in LUM & Alpha- Improved Alpha Technologies : Better S/B and S/N - AlphaPlex based on Simultaneous Dual Emission Channel
measurement - New Faster Stacker - New software features including new well scanning options- Increased Calculations options in Analysis package MARS
Acknowledgments
Our Application Specialists and Engineers in Ortenberg, Germany
The PHERAstar FSX roadshow is coming to UK
If interested please visit us at stand D1 and
put us to the test!