Intro to the Microscope

Parts of a Microscope

Parts of a Microscope

• Objective Lens: Lens that receives the first rays of light from the light source.

• 3 magnifications: Eyepiece

Objective Lens

Magnification

Low Power

10 x 10 x 100 x

Green = GO! Green = GO! Green = GO! Green = GO!

High Power

10 x 43 x 430 x

Yellow = Caution Yellow = Caution Yellow =

Caution Yellow = Caution

Oil Lens 10 x 97 x 970 x

Red = STOP!

Never Use This Lens!

or 100 x or 1000 x

Fine vs. Course Focus

Course focus- for focusing on low power objective.oONLY USE ON LOW POWER!!!

Fine focus- for use on high power objective

Using the Microscope

• Place slide so center of slide is directly over the light source.

• Always start on lower power• Use course adj. knob to get specimen in focus.• Switch to high power objective.• Use fine adj. knob to get specimen in focus.

Preparing a Wet Mount Slide

– Get this slice of specimen and place on center of slide.

– Put one drop of water over specimen.– Place coverslip at 45 degree angle and drop on

slide.