Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300...

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Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 200 300 400 500 100 200 300 400 500 100 200 100 Final Jeopardy directions on last slide Jeopardy Theme Song: http://www.tesd.k12.p a.us/technology/2001- 2002%20Web/Jeopardy.w av

Transcript of Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300...

Page 1: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous

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Final

Jeopardy

See directions on last slide

Jeopardy Theme Song: http://www.tesd.k12.pa.us/technology/2001-2002%20Web/Jeopardy.wav

Page 2: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

Intro to Biotech / Micro for 100

•The study of living tools

»What is Biotechnology?

Back

Page 3: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

Intro to Biotech / Micro for 200

•2 requirements for fermentation to occur

»What are a microbe, a sugar source, and an anaerobic environment

Back

Page 4: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

Intro to Biotech / Micro for 300

•This type of microbe has eukaryotic cells

»What are fungi.

Back

Page 5: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

Intro to Biotech / Micro for 400

• The term for bacteria with THICKER cell walls

»What is Gram +.

Back

Page 6: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

Intro to Biotech / Micro for 500

• Some antibiotics target this component of bacterial cell walls (which is why they don’t work on eukaryotic cells)

»What is peptidoglycan. Back

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Cloning and Such 100

• The reason Dolly the Sheep is significant

»What is she was the first

clone from an adult cell

Back

Page 8: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

Cloning and Such 200•The term for the way in which plants can clone themselves

»What is natural vegetative propagation

Back

Page 9: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

Cloning and Such 300•Grafting is an example of this

»What is artificial vegetative propagation.

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Page 10: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

Cloning and Such 400•The term for the ability of a cell to become any other cell type of an organism

»What totipotency. Back

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Cloning and Such 500• A type of cloning that is

done for the sole purpose of creating stem cells, NOT an organism

»What is therapeutic cloning

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Page 12: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

DNA and Protein for 100

•TATA, for example»What is a promoter.

Back

Page 13: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

DNA and Protein for 200

• This is the molecule that carries the code of DNA out of the nucleus.

»What is mRNA.

Back

Page 14: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

DNA and Protein for 300

•This is the process by which mRNA is copied from DNA

»What is transcription.

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Page 15: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

DNA to Protein 400

•This is the site in the cell where proteins are manufactured.

»What is the ribosome.

Back

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DNA to Protein 500

•This is the process by which mRNA bases code for amino acids.

»What is translation.

Back

Page 17: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

DNA Labs for 100

•A molecule found in bacteria that cuts up foreign DNA

»What is a restriction enzyme

Back

Page 18: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

DNA Labs for 200

•DNA that is a hybrid of 2 organisms

»What is recombinant DNA

Back

Page 19: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

DNA Labs for 300

•A technique for amplifying a sample of DNA

»What is PCR

Back

Page 20: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

DNA Labs for 400

Daily Double!!!

Back

•The component(s) of the media that caused a +pGlo bacterial colony to GROW and GLOW

What is LB , Amp, and Ara.

Page 21: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

DNA Labs for 500

• The optimal temperature for the extension of a DNA molecule by Taq polymerase (within 4 degrees)

»What is 72 C. Back

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Miscellaneous for 100

•A property of the GFP protein that we used to purify it.

»What is hydrophobic

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Miscellaneous for 200

•Term for the use of microbes to clean up environmental contaminants

»What is bioremediationBack

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Miscellaneous for 300

• A technique for counting the number of bacteria in a sample, even though the number may be very large

»What is serial dilution.

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Miscellaneous for 400

•A microbe that might have RNA but no DNA

»What are viruses

Back

Page 26: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

Miscellaneous for 500

•The result of adding KOH to Gram - bacteria

»Cell wall ruptures, DNA released.

Back

Page 27: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

Final Jeopardy

• Make a wager!

• Give an example of a DNA palindrome

»various

Back

Click here for the 30 second Think Song

Page 28: Intro to Biotech/Micro Cloning & Such DNA & Proteins DNA Labs Miscellaneous 300 400 500 100 200 300 400 500 100 500 400 300 200 300 400 500 100 200 300.

How to use this game:• Slide 2 is the motherboard. When you click on the amount, it will take you to the

corresponding slide. When you go back to the motherboard the amount should no longer show in black.

• Click once on the slide to view the question for that amount. • Click a second time to view the answer.• Always, always click on the back button to go back to the motherboard. If you click on the

slide it will take you to the next higher amount but it will not show that the new amount as unavailable on the motherboard.

• Animations of text are set on random so a couple can be distracting or slow. • If the text is too small on the motherboard you could have it on the board as well. • If you’d rather not do the ppt but still want the game, try going to:

Print Print What Handouts and Slides Per Page 9; or Print What outline view• Double Jeopardy requires an extra click to get the question. Students will need to either

draw and/or use the model kits to show protein synthesis. You decide. The answer is not provided. You’ll have to draw it on the board if you want it written. Don’t try and click for it. Obviously this is double the pay amount.

• Final Jeopardy can be reached by clicking on the text in the sun symbol on the motherboard.• Because the amounts turn white after you use them, unfortunately you’ll have to close the ppt

after each use to reset the amounts. • Options for how you play it with large groups are many. Do what you want and rock on!