INTERNATIONAL CONFERENCE - UMS

INTERNATIONAL CONFERENCE

ICB PHARMA II

PROGRAMME

and

ABSTRACT BOOK

“Current Breakthrough in

Pharmacy Materials and Analyses”

Faculty of Pharmacy

Universitas Muhammadiyah Surakarta

2015

ii | P a g e ICB Pharma II – 31 October 2015

“Current Breakthrough in Pharmacy Materials and Analyses”

Pharmacy Faculty, Universitas Muhammadiyah Surakarta Surakarta, Indonesia

Chairman of Editors : Erindyah Retno Wikantyasning, Ph.D., Apt. Tanti Azizah Sujono, M.Sc., Apt.

Team of Pharmaceutical Technology : Anita Sukmawati, Ph.D., Apt. Suprapto, M.Sc.,Apt. Erindyah Retno Wikantyasning, Ph.D., Apt. Gunawan Setiyadi, M.Sc., Apt.

Team of Pharmacology and Microbiology : Azis, Saifudin, Ph.D., Apt. Arifah Sri Wahyuni, M.Sc., Apt. Ratna Yuliani, M.Biotech.St. Tanti Azizah Sujono, M.Sc., Apt.

Team of Clinical and Community Pharmacy : Dra. Nurul Mutma’inah, M.Si., Apt. Zakky Cholisoh, M.Clin. Pharm., Ph.D., Apt. Hidayah Karuniawati, M.Sc. Apt.

Team of Pharmaceutical Chemistry : Dedi Hanwar, M.Si., Apt. Dr. Muhammad Da’i, M.Si., Apt. Dr. Muhtadi Ibrahim, M.Sc. Broto Santoso, M.Sc., Apt. Andi Suhendi, M.Sc., Apt. Ika Trisharyanti, M.Sc., Apt.

Team of Molecular Biology : Agus Purnomohadi, M.Sc. Maryati, Ph.D., Apt.

Copyright ©2015 Copyright in compilers and reserved Design cover: Publication and Documentation Team Layout: Secretary and IT Team

Published by: Muhammadiyah University Press Universitas Muhammadiyah Surakarta Jl. A. Yani Pabelan Tromol Pos I Kartasura Surakarta 57102 Telp. (+62 271) 717417-172, E-mail: [email protected]

ICB Pharma II – 31 October 2015 “Current Breakthrough in Pharmacy Materials and Analyses”

iii | P a g e

PREFACE

It’s my great pleasure to welcome you to the 2nd International Current

Breakthrough (ICB)-Pharma Symposium 2015 in Solo Indonesia which will be held on 31

October, 2015 under the auspices of Universitas Muhammadiyah Surakarta.

ICB-Pharma 2015 will feature a theme of “Current Breakthrough in Pharmacy

Material and Analyses” and will consist of morning and afternoon sessions. There will be

plenary lectures and session lectures given by several invited speakers from Singapore,

Japan, as well as from Indonesia, and also selected oral presentations of the submitted

papers. The poster session from various fields of pharmaceutical sciences will take place

nearby.

The ICB-Pharma will be directed for a tradition and in the future will be nurtured

as a well-known scientific symposium in disseminating the breakthrough and novel

technology in pharmacy materials. This purpose will be able to achieve by encouragement

of national and international academic institution partners and supports from the

industrial partners, Indonesian Pharmacist Association (Ikatan Apoteker Indonesia, IAI),

colleagues and from the organizing committee. Moreover, I do hope and believe that, the

2nd ICB-Pharma will offer great opportunities for the scientists to meet and discuss recent

topics in the field of material and pharmaceutical science and bring the academics, health

professionals and industries together for sharing their experiences to solve current

problems and challenges in practice.

Warm regards,

Azis Saifudin, PhD, Apt.

Chair of ICB-Pharma Symposium

iv | P a g e

ICB Pharma II – 31 October 2015


PREFACE

This conference is held to disseminate current methods which provide advanced

materials and methods in pharmacy. This is a good media for those who are engaged in

academic, industrial, regulatory fields to conduct social interactions, to share their

findings, to communicate bottle neck surrogates, and to seek the possibilities for

collaborations. We are quite humble to recognize our weakness in running all agendas.

Hence, from bottom of our heart we ask apologizes from all participants for any service,

lack facilities, low response, etc. However, we must be persistent and ensuring our

positive contribution toward scientific society especially at the attempt to develop

capacity building of pharmaceutical sciences in Indonesia. Thus, we will run ICB Pharmacy

III next year.

To all participants, I wish an inspiring moment in Solo city, a city where was born a

national leader and city of heritage!

Azis Saifudin, PhD, Apt.

Dean of Faculty of Pharmacy Universitas Muhammadiyah Surakarta


v | P a g e

TABLE OF CONTENTS

PREFACE .................................................................................................................................................................. iii

TABLE OF CONTENTS .......................................................................................................................................... v

PROGRAMME .......................................................................................................................................................... ix

ORAL PRESENTER ROOM................................................................................................................................... x

LIST OF PARTICIPANT NON PRESENTERS ................................................................................................ xi

LIST OF ORAL PRESENTERS ......................................................................................................................... xvi

LIST OF POSTER PRESENTERS .................................................................................................................. xviii

SPEAKER ................................................................................................................................................................... 1

A-PHARMACEUTICAL TECHNOLOGY ............................................................................................. 4

A001 CHARACTERISTICS TESTING OF MICROCRYSTALLINE CELLULOSE FROM NATA DE COCO COMPARED TO AVICEL pH 101 AND AVICEL pH 102

Adi Yugatama1*, Laksmi Maharani2, Hening Pratiwi2, Lingga Ikaditya3 .......................................... 4

A002 PREPARATION OF ARTIFICIAL SALIVA FORMULATION

Andi Sri Suriati Amal1*, Samsinah Hj. Hussain2, Mohd. Amin Jalaluddin3 ....................................... 5

A003 FORMULATION OF ETHANOL EXTRACT OF TUNJUK LANGIT (Helminthostachys zaylanica) RHIZOME AS TABLET DOSAGE FORM BY WET GRANULATION METHOD

Fitrya1*, Najma Annuria Fithri1, Budi Untari1, Aprililianti1 ................................................................... 6

A004 EFFECT COGRINDING ON THE PHYSICAL CHARACTERISTIC OF BINARY MIXTURE OF PIOGLITAZONE HCl AND METFORMIN HCl

Iskandar Zulkarnain1*, S. N. Soewandhi1, S. Wikarsa2 ............................................................................. 7

A005 PREPARATION AND CHARACTERIZATION OF SUBMICRON PARTICLES OF PLGA INCORPORATING RIFAMPIN USING EMULSION SOLVENT DIFFUSION METHOD

Mardiyanto1* ............................................................................................................................................................ 8

A006 LIQUID BATH SOAP FORMULATION AND ANTIBACTERIAL ACTIVITY TEST AGAINST Staphylococcus aureus OF KECOMBRANG (Etlingera elatior (Jack) R.M.Sm.) FLOS EXTRACTS

Lilis Handrayani1, Ratih Aryani1*, Indra1 ...................................................................................................... 9

A007 THE INFLUENCE OF EGGPLANT PEEL EXTRACT (Solanum melongena L.) AS ANTIOXIDANT IN LOTION DOSAGE FORM

Sholichah Rohmani1* ......................................................................................................................................... 10

A008 OPTIMIZING COMBINATION OF SAMBILOTO HERBAL WATER FRACTION AND SALAM LEAF WATER FRACTION AS ANTI-INFLAMMATION

Raymond Harris Mustafa1, Lannie Hadisoewignyo1*, Martha Ervina1, Lisa Soegianto1, Wahyu Dewi Tamayanti1 .............................................................................................................. 11

vi | P a g e



B-PHARMACOLOGY AND MICROBIOLOGY ................................................................................. 12

B001 THE POTENTIAL OF THE BLACK RICE BRAN EXTRACT AS ANTIDIABETIC AGENT

Arifah Sri Wahyuni1*, Rima Munawwaroh1, Esthi Utaminingsih1, Novita Sari1 ........................ 12

B002 ANTIOXIDANT ACTIVITIES OF NANOEMULSION CONTAINING EXTRACT OF SAMBILOTO (Andrographis paniculata (Burm F.) Ness.) AND MENIRAN (Phyllanthus niruriL.) IN ALLOXAN- INDUCED DIABETIC RATS

Erindyah R Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Da’i1, Doni Wibowo1............................................................................................................................................... 13

B003 ACUTE TOXICITY STUDY OF NANOEMULSION CONTAINING SAMBILOTO (Andrographis paniculata) LEAVES AND MENIRAN (Phyllanthus niruri) HERBS EXTRACT IN WISTAR RATS

Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Da’i1, Febby Lovita Sari1 .......................................................................................................................................... 14

B004 ANTI-INFLAMMATORY ACTIVITY TEST OF CHRISTMAS PALM (Adonidia merrillii (Becc.) Becc.) SEED EXTRACT IN MALE WISTAR RATS (Rattus norvegicus)

Herlina1*, Fitrya1, Fithri Najma A1, Rahmawati Dwi Shafarina1 ....................................................... 15

B005 THE EFFECT OF GIVING GLUCOMANNAN PORANG TUBER (Amorphophallus oncophyllus Prain ex Hook. F.) ON SGPT AND SGOT LEVELS OF MALE WISTAR RATS BLOOD INDUCED BY PARACETAMOL

Intan Martha Cahyani1*, Bekti Nugraheni1 ............................................................................................... 16

B006 THE PROFILE OF MDA (malondialdehyde) LEVEL IN RATS GIVEN EXTRACT OF THYMI HERBS (Thymus vulgaris [L.])

Ken Inayati Latifa1*, Tanti Azizah Sujono1, Ika T. Dian Kusumowati1 ........................................... 17

B007 PRE-CLINICALSTUDY OF Cr (III) BASED HYPOGLICEMIC SUPPLEMENT IN-TYPE 2 DIABETIC RATS

Kun Sri Budiasih1*, Kartika Ratna Pertiwi1 ............................................................................................... 18

B008 SCREENING ANTIBACTERIAL POTENCY OF ENDOPHYTIC FUNGI METABOLITE OF MANGOSTEEN (Garciniamangostana L.) LEAF

Lisa Soegianto1*, Martha Ervina1, Kevin Widjaja1, Angela Violita1 ................................................. 19

B009 ACTIVITIES OF THE COMBINED EXTRACTS OF TEMPUYUNG (Sonchus arvensis) AND BLACK CUMIN (NIGELLA SATIVA) AGAINST XANTHINE OXIDASE INHIBITION ON HYPERURICEMIC MICE

Muhtadi1*, Nurcahyanti Wahyuningtyas1, Andi Suhendi1, Septi Heryani1 .................................. 20

B010 ANTIBACTERIAL ACTIVITY OF COMBINATION OF CHLORAMPHENICOL AND ETHANOLIC EXTRACT OF PACAR AIR (Impatiens balsamina) LEAVES AGAINST Escherichia coli AND Shigella sonnei

Ratna Yuliani1*, Agung Cokro Prabowo1, Yeni Maisyah1..................................................................... 21

B011 ANTIHYPERCHOLESTEROLEMIC EFFECT OF Murbei (Morus alba L.) LEAVES AND ITS COMBINATION WITH SIMVASTATIN IN RATS INDUCED BY PROPYLTIOURACIL AND HIGH FAT DIET

Tanti Azizah Sujono1*, Haryoto1, Ratna Kartikasari1, Laily Ieda Quntari1 ................................... 22


vii | P a g e

B012 ANALGESIC EFFECT OF ETHANOLIC EXTRACT RED DRAGON FRUIT ( Hylocereus polyrhizus Cortex) PEEL ON MALE MICE SWISS WEBSTER WITH WRITHING REFLEX METHOD

Westi Fajrin Bayu Nugrahaini1*, Tanti Azizah Sujono1 ........................................................................ 23

B013 EFFECTS OF TURMERIC ETHANOLIC EXTRACT ON TRIMETHYLTIN-INDUCED OXIDATIVE STRESS ON SPRAGUEY DAWLEY RATS

Sapto Yuliani1*, Mustofa Mustofa2, Ginus Partadiredja2 ...................................................................... 24

B014 ANTIDIABETIC ACTIVITY OF NANOEMULSION CONTAINING EXTRACT OF SAMBILOTO (Andrographis paniculata (BURM F.) NESS.) AND MENIRAN (Phyllanthus niruri L.) IN ALLOXAN-INDUCED DIABETIC RATS .................................. 25

Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Da’i1, Fajar Kholikul Amri1 ................................................................................................................................... 25

C- CLINICAL AND COMMUNITY PHARMACY .............................................................................. 26

C001 OVERVIEW ABOUT ANTIBIOTIC’S PRESCRIBING IN URINARY TRACT INFECTION ROEMANI SEMARANG HOSPITAL’S INPATIENTS

Hening Pratiwi1* .................................................................................................................................................. 26

C002 RATIONALITY TREATMENT OF ANTIBIOTICS FOR TREATMENT OF DIARRHEA IN ADULT PATIENTS IN THE INPATIENT INSTALLATION OF HOSPITAL “X” SURAKARTA IN 2014

Ida Ayu Pebrina1*, Suharsono1, Suprapto1 ................................................................................................ 27

C003 CLINICAL IMPROVEMENT AFTER CEPHALOSPORINE THERAPY ON CHILDREN WITH TYPHOID FEVER

Rasmaya Niruri1*, N.P. Yulia Purnami1, Julius D.Tansale2,3, I.B.Eka Erlangga3 .......................... 28

C004 TRANSAMINITIS ASSOCIATED WITH HIGH DOSE METHOTREXATE AND 6-MERCAPTOPURIN IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA

Rasmaya Niruri1*, K. Trisna Komalasari1, Ketut Ariawati2 ................................................................ 29

C005 TREATMENT AND COST ANALYSIS OF DIARRHEA PATIENTS OF INPATIENT INSTALLATION OF RSUD dr. MOEWARDI SURAKARTA BY BPJS PROGRAM IN 2014

Saktya Ayu Donna P1*, Suharsono1, Suprapto1........................................................................................ 30

D-PHARMACEUTICAL CHEMISTRY ............................................................................................... 31

D001 ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF PARKIA SEED AND POD (Parkia speciosa Hassk.) BY DPPH (1,1-DIPHENYL-2- PICRYLHYDRAZYL) METHOD .............................................................................................................................................. 31

Indri Kusuma Dewi1*, Agus Winarso1 ......................................................................................................... 31

D002 A CLICK-TYPE COUPLING REACTION BETWEEN THIOAMIDES AND SULFONYL AZIDES AS A VERSATILE APPROACH TO GENERATE NEW PHARMACOLOGICALLY ACTIVE COMPOUNDS ................................................................... 32

Muhammad Aswad1, Junya Chiba1*,Yasumaru Hatanaka1, Takenori Tomohiro1 ..................... 32

D003 ANTIBACTERIAL COMPOUND PRODUCED BY A SOIL BACTERIA ISOLATED FROM RIZHOSPHERE OF Zingiber officinale....................................................................................... 33

viii | P a g e



Nanik Sulistyani1,2*, Yosi Bayu Murti3, Jaka Widada4, Mustofa5 ....................................................... 33

E-MOLECULAR BIOLOGY .................................................................................................................. 34

E001 CYTOTOXIC ACTIVITY OF POLAR, SEMIPOLAR, AND NON POLAR FRACTION OF ETHANOL EXTRACT OF SALA PLANTS LEAVES (Cynometra ramiflora Linn.) AGAINTS WiDr CELL

Haryoto1*, Anis N. Irjayanti1, Tanti Azizah Sujono1, Muhtadi1, Andi Suhendi1 .......................... 34

E002 CHALCONES DERIVATIVE WITH BROMO SUBSTITUENT INDUCES APOPTOSIS IN HeLa CELLS

Retno Arianingrum1*, Indyah Sulistyo Arty1 ............................................................................................ 35

E003 SUB-CLONING OF ads GENE INTO pETDUET1_cyp FOR CO-EXPRESSION IN ESCHERICHIA COLI

Imam A. Wicaksono1, Tresna Lestari2*, Evi U. Ulfa3, Catur Riyani1, Elfahmi1 ............................. 36

CV OF SPEAKER ................................................................................................................................................... 37

LIST OF COMMITTEES ...................................................................................................................................... 40

MAP OF FACULTY OF PHARMACY ............................................................................................................... 42

LIST OF TAXI AND HOTELS ............................................................................................................................ 43


ix | P a g e

PROGRAMME

Venue: Auditorium Moh. Djazman, Universitas Muhammadiyah Surakarta Jl. Ahmad Yani, Tromol Pos I, Pabelan, Surakarta 57162 Indonesia

Session: Morning Session

07.00-08.00 Registration for participant

08.00-08.30 Opening Ceremony

08.30-09.00 Morning Coffee Break

09.00-09.45 Speaker 1 Prof. Jackie Ying (Executive Director, Institute of Bio-engineering and Nanotechnology) Topic: "Current status on nano medicine and nano devices"

09.45-10.30 Speaker 2 Assoc. Prof. Takenori Tomohiro (University of Toyama, Japan). Topic: "Photoaffinity labeling-based target identification of bioactive molecules"

10.30-11-15 Speaker 3 Dr. Wangsa Tirta Ismaya (Scientist/Head section of Recombinant therapeutic proteins, PT. Dexa Medica). Topic: "Current status of recombinant therapeutic proteins and local issues related"

11.15-12.00 Discussion

12.00-13.00 Lunch break

Afternoon Session

13.00-13.30 Poster Session

13.30-16.00 Oral Presentation Session

16.00-16.30 Closing Ceremony

x | P a g e



ORAL PRESENTER ROOM

Room Code Presenter Moderator

K4-A

A001 ADI YUGATAMA

Gunawan Setiyadi, M.Sc., Apt.

A003 FITRYA

A005 MARDIYANTO

A006 RATIH ARYANI

A008

K4-B

C001 HENING PRATIWI

Andi Suhendi, MSc., Apt. C003 RASMAYA NIRURI

D001 INDRI KUSUMA DEWI

D002 MUHAMMAD ASWAD

K4-C

B001 ARIFAH WAHYUNI

Dedi Hanwar, M.Si., Apt.

B004 HERLINA

B007 KUN BUDIASIH

B009 MUHTADI

B011 TANTI AZIZAH

K4-D

B008 LISA SOEGIANTO

Maryati, Ph.D., Apt.

B010 RATNA YULIANI

E001 HARYOTO

E002 RETNO ARIANINGRUM

E003 TRESNA LESTARI


xi | P a g e

LIST OF PARTICIPANT NON PRESENTERS

No Name Institution

1 ARDIANSAH UMS

2 HIMYATUL HIDAYAH PRODI MAGISTER FARMASI UMS

3 MARIA ULFA PRODI MAGISTER FARMASI UMS

4 MARIA ULFA ANANDA FARMA

5 ABDUL RONI UMS

6 MUSTAKIM MASNUR UMS

7 DITA MARINA LUPITANINGRUM PRODI MAGISTER FARMASI UMS

8 UMI KHOLIFAH.S.FARM.,APT APOTEK KARUNIA FARMA

9 LENI HERLINA WIDIASARI, S.SI.,APT. APOTEK WALI SEHAT SEMARANG

10 DHIGNA LUTHFIYANI CITRA PRADANA

UNIVERSITAS JAMBI

11 YUSNITA APRILIYANA APOTEK SIDAPURNA

12 NOVI DIANASARI, S.FARM, APT. APOTIK KONDANG WARAS

13 DEVI UTAMI YULISTYANTI S. FARM., APT

ELLA SKIN CARE SOLO

14 DIKA HERNAWATI S.FARM., APT ELLA SKIN CARE SOLO

15 ARIS SETIAWAN KLINIK INSANI MEDICA

16 UNGKI PRASETYO KLINIK RETNO

17 MARIA RINA DWI SUSILOWATI.,S.SI.,APT

PT. GRAHA FARMA

18 DRA. DWI MURTINGHASTUTI,APT PT. GRAHA FARMA

19 AGNES RINA SRI MURWANI, S.SI.,APT

PT. GRAHA FARMA

20 DWI KARTIKA SANTI RS PKU MUHAMMADIYA KARTASURA

21 SRI HARTINI, S.FARM.APT APOTEK SUMBER SEHAT BOYOLALI

22 NUR SEKTI HIDAYAT RAHMAWATI S. FARM., APT.

UMS

23 DIAZ VEGA AKHIRUNNISA, S.FARM., APT

LBC WONOSOBO

24 ANNISA RIZKI MURDIYANI PT. DAYA MUDA AGUNG

25 UMI KHOLIFAH.S.FARM.,APT APOTEK KARUNIA FARMA

26 HANDIKA DESI YANOTAMA SMK ASSHODIQIYAH SEMARANG

27 RETNO HARI WAHYUNI BPOM RI

28 CAESNAN MARENDRA GRAHAN LEDITTO

UNIVERSITAS KRISTEN IMMANUEL

29 SARAH PUSPITA ATMAJA AIRLANGGA UNIVERSITY

30 MEILANA SUSANTI APOTEK HARJOSARI FARMA

31 ULFAHTUL LAELI APOTEK DAWA FARMA

32 INAYATUL HIDAYATI APOTEK SERAYA

33 LULU BACHRIYAH APOTEK MUTIARA

xii | P a g e



No Name Institution

34 MARLIN TIGOR S.SI. APT APOTEK PUTRID

35 LENI HERLINA WIDIASARI SSI.,APT APOTIK WALI SEHAT

36 ARRYSKA AYU PERMATASARI S.FARM.,APT

IAI KAB TEGAL

37 MALICHATUN APOTEK KAROMAH

38 RIZAMDHANI APOTEK KIRANI

39 LINNA MARLINNA S.SI, APT IAI PC KAB. TEGAL

40 HENDRA ANTAN DJAYA, S.SI, MBA, APT

IAI PC KAB. TEGAL

41 QUROTUL AINI, S.FARM.,APT APOTEK MITRA MEDIKA

42 IRA RAHMIYANI STIKES BAKTI TUNAS HUSADA TASIKMALAYA

43 FRANSISCA MEINDRIA NARASTY, S.FARM., APT.

PT. GRAHA FARMA SURAKARTA

44 DESTANTIA NADYA AMANTHA S.FARM., APT

STIFAR YAYASAN PHARMASI SEMARANG

45 TYAS SARININGRUM S.FARM., APT STIFAR YAYASAN PHARMASI SEMARANG

46 CANDRA EKA PUSPITASARI UNIVERSITAS GADJAH MADA

47 DHIGNA LUTHFIYANI CITRA PRADANA

UNIVERSITAS JAMBI

48 FARIDHA CYNTHIA DHEWANTI UMS

49 SITI CHOTIAH UMS

50 AMIRA UMS

51 TRI YOGO WIBOWO UMS

52 AHMAD RIFQI KURNIA UMS

53 ABDUL HADI UMS

54 NUR ALFIAWATI UMS

55 CHOIRUL MA'ARIF UMS

56 RAFA ENBUN RELIGIA UMS

57 SOVY SAPTA NUARI PRAMOLIS UMS

58 NAUFAL SATRIA HUTOMO UMS

59 HESTU PUTRI MITAYANI UMS

60 SANGGITA AYU IKASARI UMS

61 FAHMI AZHARI UMS

62 DYAH RISWARI PITALOKA UMS

63 ALIFAH ANASTYA DINI UMS

64 RANI UTAMI WIDYANINGRUM UMS

65 MARHAMAH NUR AZIZAH UMS

66 DRAJAT TRI WAHYUDI UMS

67 LITA RAHIMA OENSJAR UMS

68 EKHWAN TRIS WANTO UMS

69 ANDHIKA RIZKY GILANG MAHAPUTRA

UMS

70 ULITYATINING TIASARI UMS


xiii | P a g e

No Name Institution

71 AMALIYAH DINA ANGGRAENI UMS

72 WIWIK NURYANI UMS

73 AGUNG COKRO PRABOWO UMS

74 ESTUNINGTYAS AYU HAPSARI UMS

75 RISQIE CAHYANING KUSUMASARI UMS

76 IRHAMADI MALIK UMS

77 YENI MAISYAH UMS

78 UMUL BAROROTUY SYAMS UMS

79 UMI NURHAYATI UMS

80 RIZKI OCTAVIANA UMS

81 BAIQ SUPRAMONIKA UMS

82 MUTMAINNAH UMS

83 HAZRINI TANJUNG SARI UMS

84 RENY WIDYA ESTUTI UMS

85 ABULKHAIR ABDULLAH UMS

86 SHIFA SILFIA UMS

87 NURYATI KUMAN UMS

88 FITRIANISA FATHUROHMAH UMS

89 IMROATUL CHASANAH UMS

90 MARWIANI ARUM SARI UMS

91 RAHAYU DWI KURNIAWATI UMS

92 ALISA PRIHARSI UMS

93 JANIKA SUJI KUSUMAWARDANI UMS

94 AYU ANGGRAENY UMS

95 EMAMATUL KHUTSIYAH UMS

96 BUNGA INTAN SAVITRI UMS

97 ANNIE RAHMATILLAH UMS

98 ISTIQAMATUSH SHOLIHAH UMS

99 ANGGRIANA ARISTYA UMS

100 ISNAINI NUR HIDAYAH UMS

101 NAHYATU SUFIAH UMS

102 RAUDAH PUTERI ALMINA UMS

103 ADIVA TANTYAS AURORA UMS

104 FADILA KHOIRUNNISA UMS

105 SARI WIJAYANTI UMS

106 INTAN RIA NURJANAH UMS

107 SITI PURWATI UMS

108 SUNJAWA ALIF SAPUTRI UMS

109 KURNIA PUNGKY ASMORO UMS

110 NIKEN DWI MULYASARI UMS

xiv | P a g e



No Name Institution

111 AISYAH PUTRIANI UMS

112 FADHILA DIAH SUMINAR UMS

113 SEKAR PUJI UTAMI UMS

114 WITRI DYAH MEILANI UMS

115 NINA NURMITA HABSARI UMS

116 VERANTIKA DEA INDRASWARI UMS

117 TRI AGUS SAROSO UMS

118 YUDA MARSONO UMS

119 AULIA ANNUR AISYIAH UMS

120 ADHI WARDHANA AMRULLAH UMS

121 FEBRIANNA SURYANINGTYAS UMS

122 ANINDYA SETYOWATI UMS

123 NOVITA SARI UMS

124 RATNA KARTIKASARI UMS

125 ESTHI UTAMININGSIH UMS

126 YENY DWI NOVITASARI UMS

127 NENI LUGKI NIAN TARY UMS

128 SALLY ASTYA UTAMI UMS

129 TANTIN HAYU SURYA UMS

130 THORIQ MAHMUD UMS

131 RAKIH YUSMA RANGGA UMS

132 HENDRA YUANA UMS

133 GITA AYU PRADINA UMS

134 BERNADI WICAKSONO UMS

135 ROSMA FAUZIAH UMS

136 TESAR ZULMI ANTORO UMS

137 ISTI SETIA HAPSARI UMS

138 BENY DWI HATMOKO UMS

139 FIKA RIZQIYANA UMS

140 ANGGITA SEKAR ARUMSASI UMS

141 DIAN YULISTIA ASTRI UMS

142 DIAN AYU ARA ARA ARTHASARI UMS

143 EKA PRASNAPARAMITA W UMS

144 RIZKA ASTIKAH FITRIANA UMS

145 NUNGKY ASMARANING WAHYONO UMS

146 DEVI AMBARRINI WAHYUNINGTYAS UMS

147 DESTY RIRIN ROHMAWATI UMS

148 EKA PRADITA PUTRI UMS

149 AKHMAD AMINUR RIZKI UMS

150 LAILY IEDA QUNTARI UMS

151 MUHAMMAD PRIYADI UMS


xv | P a g e

No Name Institution

152 MANGGAR ARUM SHINTYA M. UMS

153 DWI SETIANINGRUM MAKUNTI UMS

154 TITIS MUTALIKAH UMS

155 MARIA ULFA UMS

156 EKA SETIANISA UMS

157 ORLAN PAKAMBANAN UMS

158 APRILYA SRI RACHMAYANTI UMS

159 DIAN RAHMAWATI UMS

160 DWI SARYANTI AKADEMI FARMASI NASIONAL SURAKARTA

161 SALSABIELA DWIYUDRISA SUYUDI UMS

162 NORA FAUZIAH UMS

163 RINI YULIANA UMS

164 LILIS FITRIATUN NISA UMS

165 EDI SUTARMANTO PRODI MAGISTER FARMASI UMS

166 NURLELA IKAWATI PRODI MAGISTER FARMASI UMS

167 INES NURFITRIANA PRODI MAGISTER FARMASI UMS

168 WULAN PRIATIWI PRODI MAGISTER FARMASI UMS

169 KATHLEEN APRIANA KRISTININGRUM JAHAMOU

UMS

170 YULI FITRIANA PRODI MAGISTER FARMASI UMS

171 HALIDA SURYADINI UMS

172 PUTRI RAMDANIAH PRODI MAGISTER FARMASI UMS

173 CHANDRA EKA PUSPITASARI UGM

xvi | P a g e



LIST OF ORAL PRESENTERS

No Code Presenter Institution Title

1 A001 ADI YUGATAMA UNIV. SEBELAS MARET

CHARACTERISTICS TESTING OF MICROCRYSTALLINE CELLULOSE FROM NATA DE COCO COMPARED TO AVICEL pH 101 AND AVICEL pH 102

2 A003 FITRYA SRIWIJAYA UNIVERSITY

FORMULATION OF ETHANOL EXTRACT OF TUNJUK LANGIT (Helminthostachys zaylanica) RHIZOME AS TABLET DOSAGE FORM BY WET GRANULATION METHOD

3 A005 MARDIYANTO SRIWIJAYA UNIVERSITY

PREPARATION AND CHARACTERIZATION OF SUBMICRON PARTICLES OF PLGA INCORPORATING RIFAMPIN USING EMULSION SOLVENT DIFFUSION METHOD

4 A006 RATIH ARYANI STIKES BAKTI TUNAS HUSADA

LIQUID BATH SOAP FORMULATION AND ANTIBACTERIAL ACTIVITY TEST AGAINST Staphylococcus aureus OF KECOMBRANG (Etlingera elatior (Jack) R.M.Sm.) FLOS EXTRACTS

5 A008 RAYMOND HARRIS MUSTAFA

WIDYA MANDALA SURABAYA CATHOLIC UNIVERSITY

OPTIMIZING COMBINATION OF SAMBILOTO HERBAL WATER FRACTION AND SALAM LEAF WATER FRACTION AS ANTI-INFLAMMATION

6 B001 ARIFAH WAHYUNI

UMS THE POTENTIAL OF THE BLACK RICE BRAN EXTRACT AS ANTIDIABETIC AGENT

7 B004 HERLINA SRIWIJAYA UNIVERSITY

ANTI-INFLAMMATORY ACTIVITY TEST OF CHRISTMAS PALM (Adonidia merrillii (Becc.) Becc.) SEED EXTRACT IN MALE WISTAR RATS (Rattus norvegicus)

8 B007 KUN BUDIASIH UNY PRE-CLINICAL STUDY OF CR (III) BASED HYPOGLICEMIC SUPPLEMENT IN –TYPE 2 DIABETIC RATS

9 B008 LISA SOEGIANTO WIDYA MANDALA SURABAYA CATHOLIC UNIVERSITY

SCREENING ANTIBACTERIAL POTENCY OF METABOLITE ENDOPHYTIC FUNGI MANGOSTEEN (Garcinia mangostana L.) LEAF

10 B009 MUHTADI UMS ACTIVITIES OF THE COMBINED EXTRACTS OF TEMPUYUNG (Sonchus arvensis) AND BLACK CUMIN (Nigella sativa) AGAINST XANTHINE OXIDASE INHIBITION ON HYPERURICEMIC MICE

11 B010 RATNA YULIANI UMS ANTIBACTERIAL ACTIVITY OF COMBINATION OF CHLORAMPHENICOL AND ETHANOLIC EXTRACT OF PACAR AIR (Impatiens balsamina) LEAVES AGAINST Escherichia coli AND Shigella sonnei

12 B011 TANTI AZIZAH UMS ANTIHYPERCHOLESTEROLEMIC EFFECT OF MURBEI (Morus alba L.) LEAVES AND ITS COMBINATION WITH SIMVASTATIN IN RATS INDUCED BY PROPYLTIOURACIL AND HIGH FAT DIET


xvii | P a g e


13 C001 HENING PRATIWI

UNSOED OVERVIEW ABOUT ANTIBIOTIC’S PRESCRIBING IN URINARY TRACT INFECTION ROEMANI SEMARANG HOSPITAL’S INPATIENTS

14 C003 RASMAYA NIRURI

UDAYANA UNIVERSITY

CLINICAL IMPROVEMENT AFTER CEPHALOSPORINE THERAPY ON CHILDREN WITH TYPHOID FEVER

15 D001 INDRI KUSUMA DEWI

POLTEKKES KEMENKES SURAKARTA

ANTIOXIDANT ACTIVITY ETHYL ACETATE EXTRACT OF SEED AND POD PARKIA (Parkia speciosa Hassk.) WITH DPPH (1,1 - DIPHENYL - 2 - PICRYLHYDRAZIL) METHOD

16 D002 MUHAMMAD ASWAD

TOYAMA UNIVERSITY

A CLICK-TYPE COUPLING REACTION BETWEEN THIOAMIDES AND SULFONYL AZIDES AS A VERSATILE APPROACH TO GENERATE NEW PHARMACOLOGICALLY ACTIVE COMPUNDS

17 E001 HARYOTO UMS CYTOTOXIC ACTIVITY OF POLAR, SEMIPOLAR, AND NON POLAR FRACTION ETHANOL EXTRACT OF LEAVES PLANTS SALA (Cynometra ramiflora Linn.) AGAINTS WiDr CELL

18 E002 RETNO ARIANINGRUM

UNY CHALCONES DERIVATIVE WITH BROMO SUBSTITUENT INDUCES APOPTOSIS IN HeLaA CELLS

19 E003 TRESNA LESTARI

STIKES BAKTI TUNAS HUSADA

SUB-CLONING OF ads GENE INTO pETDUET1_cyp FOR CO-EXPRESSION IN ESCHERICHIA COLI

xviii | P a g e



LIST OF POSTER PRESENTERS


1 A002 ANDI SRI SURIATI AMAL

UNIV. DARUSSALAM GONTOR

PREPARATION OF ARTIFICIAL SALIVA FORMULATION

2 A004 ISKANDAR ZULKARNAIN

UNIV. MUSLIM INDONESIA

EFFECT COGRINDING ON THE PHYSICAL CHARACTERISTIC OF BINARY MIXTURE OF PIOGLITAZONE HCl AND METFORMIN HCL

3 A007 SHOLICHAH ROHMANI

UNIV. SEBELAS MARET

THE INFLUENCE OF EGGPLANT PEEL EXTRACT (Solanum melongena L.) AS ANTIOXIDANT ON THE LOTION MATERIAL

4 B002 DONI WIBOWO UMS ANTIOXIDANT ACTIVITIES OF NANOEMULSION CONTAINING EXTRACT OF SAMBILOTO (Andrographis paniculata (Burm F.) Ness.) AND MENIRAN (Phyllanthus niruri L.) IN ALLOXAN- INDUCED DIABETIC RATS

5 B003 FEBBY LOVITA SARI

UMS ACUTE TOXICITY STUDY OF NANOEMULSION CONTAINING SAMBILOTO (Andrographis paniculata) LEAVES AND MENIRAN (Phyllanthus niruri) HERBS EXTRACT IN WISTAR RATS

6 B005 INTAN CAHYANI STIFAR YAYASAN PHARMASI

THE EFFECT OF GIVING GLUCOMANNAN PORANG TUBER (Amorphophallus oncophyllus Prain ex Hook. F.) ON SGPT AND SGOT LEVELS OF WISTAR MALE RATS BLOOD INDUCED BY PARACETAMOL

7 B006 KEN LATIFA UMS THE PROFILE OF MDA (MALONDIALDEHYDE) LEVEL IN RATS GIVEN EXTRACT OF THYMI HERBS (Thymus vulgaris [L.])

8 B012 WESTI FAJRIN BAYU NUGRAHAINI

UMS EFFECTIVENESS TEST of 70% ETHANOL EXTRACT ANALGETIK RED DRAGON FRUIT PEEL (Hylocereus polyrhizus Cortex) WITH STRETCHING METHOD ON MALE MICE SWISS WEBSTER STRAIN

9 B013 SAPTO YULIANI UAD EFFECTS OF TURMERIC ETHANOLIC EXTRACT ON TRIMETHYLTIN-INDUCED OXIDATIVE STRESS ON SPRAGUEY DAWLEY RATS

10 B014 FAJAR KHOLIKUL AMRI

UMS ANTIDIABETIC ACTIVITY OF NANOEMULSION CONTAINING EXTRACT OF SAMBILOTO (Andrographis paniculata (BURM F.) NESS.) AND MENIRAN (Phyllanthus niruri L.) IN ALLOXAN-INDUCED DIABETIC RATS

11 C002 IDA AYU PEBRINA UMS RATIONALITY TREATMENT OF ANTIBIOTICS FOR TREATMENT OF DIARRHEA IN ADULT PATIENTS IN THE INSTALLATION INPATIENT HOSPITAL “X” SURAKARTA 2014


xix | P a g e


12 C004 RASMAYA NIRURI UDAYANA UNIVERSITY

TRANSAMINITIS ASSOCIATED WITH HIGH DOSE METHOTREXATE AND 6 MERCAPTOPURIN IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA

13 C005 SAKTYA AYU PRACILLIA

UMS COST ANALYSIS AND DESCRIPTION TREATMENT OF DIARRHEA IN PATIENTS OF INPATIENT HOSPITAL dr. MOEWARDI SURAKARTA BY BPJS PROGRAM IN 2014

14 D003 NANIK SULISTYANI

UMS ANTIBACTERIAL COMPOUND PRODUCED BY A SOIL BACTERIA ISOLATED FROM RIZHOSPHERE OF Zingiber officinale

../../../../../../ACEGRO~1/AppData/Local/Temp/Rar$DI01.603/daftar%20oral%20n%20poster%20presenter.xlsx#RANGE!_Toc432925913





1 | P a g e

SPEAKERS

NANOSTRUCTURED BIOMATERIALS FOR MEDICAL AND BIOLOGICAL APPLICATIONS

Jackie Y. Ying

Institute of Bioengineering and Nanotechnology 31 Biopolis Way, The Nanos, Singapore 138669

E-mail: [email protected] Nanostructured materials have been developed for various medical and biological applications. They have been designed as stimuli-responsive drug delivery systems and sustained protein delivery systems. Nanocomposite systems have also been derived to provide simultaneous drug delivery and bioimaging functions as theranostic systems. Micellar nanocomplexes have been synthesized with green tea-based ingredients as unique carrier materials that offer synergistic therapeutic effects with the drugs to be delivered. In addition, nanostructure processing has been employed in creating synthetic cell culture substrates for the expansion and controlled differentiation of stem cells. Nanostructured scaffolds have also been obtained for cell and tissue engineering.

2 | P a g e



SPEAKERS

PHOTOAFFINITY LABELING-BASED TARGET IDENTIFICATION OF BIOACTIVE MOLECULES

Takenori Tomohiro

Graduate School of Medicine and Pharmaceutical Sciences University of Toyama

Email: [email protected]

Target identification and confirmation for small molecules is often the rate-limiting step in drug discovery. Photoaffinity labeling (PAL) is a photochemical method to attach a tag with a covalent bond into the ligand-interacting surface within target protein. Since PAL can access to most of biological interacting systems including membrane protein or weak-binding protein that are difficult to access by conventional affinity purification methods, it may be a powerful method for identification of target protein that is also able to determine its interacting site. However, conventional PAL-based identification method accompanied with isolation of the target in very pure form often failed, especially in case of the target of a trace amount. The purification process is often complicated by inevitable contamination that is due to non-specific adsorption and significant loss during handling because of the different physical properties of individual peptide fragments. Recently, we designed a unique photoreactive unit that can install a high-performance chemical tag, an isotope-coded fluorescent tag, to the interacting surface upon UV-irradiation. The combination of PAL and heterogeneous target-selecting techniques significantly simplified the procedure and reduced the amount of quantity and time required for identification. The details will be presented.


3 | P a g e

SPEAKERS

CURRENT STATUS OF RECOMBINANT THERAPEUTIC PROTEINS AND LOCAL ISSUES RELATED

Wangsa Tirta Ismaya

Recombinant Therapeutic Proteins, Dexa Laboratories of Molecular Sciences, Dexa Medica Industri Selatan V PP-7, Jababeka II Industrial Estate

Cikarang 17550, Indonesia E-mail: [email protected]

Generation of recombinant version of a therapeutic protein (rTP) has been long employed to solve issues with availability of the protein from its natural resource. Such approach results in production of the protein in large scale of controlled, engineered, and directed manner. However, cost of both generation and production of the biological drug is high, thereby protein theraphy is still not accessible for patients of limited income. Nevertheless, numerous rTPs have been produced in the past decades and their demands keep on increasing. Expiration of patents protecting current rTP allows production of its generic version called biosimilar, which can be made available at lower prices from reduced costs of product development and of pre-clinical and clinical trials. Production of biosimilar is done through processes obtained from technology transfer or self-developed. Either way, validation of the biosimilar product remains the major challenge. Biosimilar product must share identical characteristics and molecular properties, and display similar safety and potency features to those of originator. Alterations in the physico-chemical properties of the molecule or improvement in potency of the drugs are not permitted. On the other hands, development of new recombinant therapeutic protein continues as new diseases or new variant of a disease emerge as well as reoccurrence of well-known diseases. Developing a recombinant therapeutic protein is usually started with search of candidates based on putative or known molecular basis of a disease. We are currently developing a new approach by means of employing proteins of unknown function to screen for their potential use in pharmaceutic or therapy. This approach involves various techniques in bioinformatics, biochemistry, molecular biology and pharmacology, and cell physiology. This approach may lead to discovery of novel pharmaceutical or therapeutic proteins. With the finding of many genes that encode proteins of unknown function during elucidation of genomes (e.g. human), this unusual approach provides alternative to discover new pharmaceutical/therapeutic proteins. We may as well assign possible biological function of the proteins. In Indonesia, development of biosimilar or recombinant therapeutic protein is still at infancy. However, pharmaceutical industries embrace development and production of biosimilar, especially to anticipate the coming Asean Economic Community implementation. The National Agency for Food and Drugs Control (NAFDC) has been developing and preparing guidelines to regulate and control the development and production of both biosimilar and rTP. Related to Indonesian moslem population in particular, development and production of halal certified drugs, including rTP and biosimilar are also part of the major issues. Especially after recently the bill for halal consumer products has been passed. Thus, development of recombinant therapeutic protein appears to gain its momentum in Indonesia in the near future.

4 | P a g e



OP A001 PHARMACEUTICAL TECHNOLOGY

CHARACTERISTICS TESTING OF MICROCRYSTALLINE CELLULOSE FROM NATA DE COCO COMPARED TO AVICEL pH 101 AND AVICEL pH 102

Adi Yugatama1*, Laksmi Maharani2, Hening Pratiwi2, Lingga Ikaditya3 1Farmasi, FMIPA, Universitas Sebelas Maret, Surakarta

2Farmasi, FIKES, Universitas Jenderal Soedirman, Purwokerto 3Farmasi, Poltekkes Kemenkes Tasikmalaya, Tasikmalaya

*E-mail: [email protected]

Abstract

Microcrystalline cellulose is an imported raw material in Indonesia, which used widely

as an excipient in tablet production. One of the alternative materials to produce microcrystalline cellulose is from nata de coco. This research aimed to know the characteristic of microcrystalline cellulose from nata de coco compared to avicel pH 101 and avicel pH 102. Nata de coco were alkalinated, dried and hydrolyzed to get microcrystalline cellulose. Independent variables in this research are microcrystalline cellulose from nata de coco, avicel pH 101 and avicel pH 102. While the dependent variables are flow properties, compactibility, compressibility, water absorption, tap density, bulk density, loss of drying, infrared absorption spectra, and SEM images. Data was analyzed using one way ANAVA with CI 95% and using software SPSS for windows. The result showed that the characteristic of microcrystalline cellulose from nata de coco is different in flow properties, compactibility, compressibility, tap density, bulk density, and loss of drying from avicel pH 101 and avicel pH 102, but having the same water absorption. Infrared spectrum data showed that microcrystalline cellulose from nata de coco is similar to avicel pH 101 and avicel pH 102. The SEM result showed that microcrystalline cellulose from nata de coco having bigger particle size (66.67–266.67 μm) than avicel pH 101 (13.33–166.67 μm) and avicel pH 102 (13.33–200 μm).

Keywords: Avicel pH 101, Avicel pH 102, Nata de coco, microcrystalline cellulose.


5 | P a g e

PP A002 PHARMACEUTICAL TECHNOLOGY

PREPARATION OF ARTIFICIAL SALIVA FORMULATION

Andi Sri Suriati Amal1*, Samsinah Hj. Hussain2, Mohd. Amin Jalaluddin3

1Universitas Darussalam Gontor, Indonesia 2Universiti Malaya, Malaysia 3Universiti Malaya, Malaysia


Abstract

Dry mouth or throat (xerostomia) is a clinical condition characterized by desiccation of

the intraoral tissues. Patients with chronic or temporary sensation of dry mouth need some kind of treatment to relieve the symptoms. Causes of dry mouth include medications, autoimmune disease (Sjogren’s syndrome), radiotherapy or chemotherapy for cancer, hormone disorders and infections. The project is important not only because saliva substitutes are not manufactured locally, but also because most saliva substitutes use mucin (porcin in origin). Therefore there is a need to produce one with other source which has properties to mucin itself. The objective of this project is to produce saliva substitutes that can serve as mouth and throat lubricants. The first step was pre-formulation studies that involved characterization of active ingredients (physical, chemical, and mechanical properties) in order to choose what other ingredients (excipients) should be used in the preparation. Formulation studies also considered such factors as solubility, viscosity, and pH. The last step was assessment of safety and stability of the final product.The new artificial saliva formulations containing various ratios of SCMC (Sodium carboxymethyl cellulose), MC (methyl cellulose) and HPMC (hydroxypropyl methycellulose) have been developed. Combination of cellulose derivatives and albumin in these formulations resulted in the physical properties of these new artificial saliva substitutes closely resembling human saliva and mucin-based saliva substitutes. Formula we choose were the most suitable formulae due to their viscosity and pH properties which closely resemble human saliva and mucin based saliva substitutes. Keywords: artificial saliva, saliva substitute, mouth and throat lubricant, mouth and throat moisturizer.

6 | P a g e




FORMULATION OF ETHANOL EXTRACT OF TUNJUK LANGIT (Helminthostachys zaylanica) RHIZOME AS TABLET DOSAGE FORM BY WET GRANULATION METHOD

Fitrya1*, Najma Annuria Fithri1, Budi Untari1, Aprililianti1 1Department of Pharmacy, University of Sriwijaya

Indralaya, OI, South Sumatera, Indonesia


Abstract

Tunjuk langit rhizome (Helminthostahcys zeylanica (Linn) Hook) has been used traditionally as an anticancer and antiinflamatory agent. In addition, previous studies have proven the potency of ethanol extract from the tunjuk langit rhizome (Helminthostahcys zeylanica (Linn) Hook) as antihyperuricemic agent. In this study, ethanol extract of the rhizome was formulated into a tablet dosage form by a wet granulation method. The tablet was prepared with different types of disintegrant and binder, i.e. Formula A (starch: PVA), Formula B (Avicel®PH102: PVP), and Formula C (sodium alginate: methylcellulose). Physical properties (such as weight variation, tablet diameter, thickness, friability, hardness, and disintegration time) and dissolution of tablets were evaluated. The results showed that tablet A (starch: PVA) produced the best physical properties and dissolution characteristics, which have met the requirements. Therefore, the wet granulation method is suitable to develop the extract into tablet. Keywords: extract, tunjuk langit rhizome, Helminthostachys zaylanica, tablet, wet granulation.


7 | P a g e


EFFECT COGRINDING ON THE PHYSICAL CHARACTERISTIC OF BINARY MIXTURE OF PIOGLITAZONE HCl AND METFORMIN HCl

Iskandar Zulkarnain1*, S. N. Soewandhi1, S. Wikarsa2

1Fakultas Farmasi Universitas Muslim Indonesia 2Sekolah Farmasi Institut Teknologi Bandung


Abstract

This study was aimed to identify the physical interaction between PGZ-MFN by

cogrinding. Solid state interaction was observed by cold contact method and phase diagram formation. The solid state grinding (SSG) and solvent drop grinding (SDG) was conducted on binary mixtures. The identification resulted in a binary system was characterized by differential thermal analysis (DTA), polarization microscopy, scanning electron microscope (SEM) and powder X-ray diffraction (PXRD). Furthermore, the solubility and dissolution testing of PGZ performed on the physical interaction results. The observation under the polarizing microscope showed that the new crystal habit was not found. The mixture has melting point lower than MFN and PGZ. This phenomenon was then confirmed with phase diagram arranged by thermogram of PGZ-MFN. That was identified mixture binary equimolar as eutectic mixture with eutectic temperature 187 °C. Meanwhile, PXRD data at equimolar mixture did not showed the new interference peak. The DTA and powder X-ray diffraction data of the equimolar solid compounds obtained from several tehnique showed similar result. The thermogram of all treated sampels had similar endothermic curve (185- 186C), and identical interference peaks of PGZ-MFN of 2θ 8.6; 12.2; 12.8; 17.2; 18.6; 22.7; 23.2 . Conclusion Based on the results of morphological analysis, the PXRD data and thermal properties, PGZ-MFN equimolar mixture showed the formation of a eutectic mixture. Keywords: Cogrinding, physical characteristic, binary system, pioglitazone HCl, metformin HCl.

mailto:[email protected]

8 | P a g e




PREPARATION AND CHARACTERIZATION OF SUBMICRON PARTICLES OF PLGA INCORPORATING RIFAMPIN USING EMULSION SOLVENT DIFFUSION METHOD

Mardiyanto1*

1Department of Pharmacy, Faculty of Mathematics and Natural Science, University of Sriwijaya Inderalaya, Indonesia


Abstract

The research had been performed to incorporate rifampin into PLGA submicron-sized

particles. This research has a prospect to be applied to overcome the ineffectiveness use of rifampin for tuberculosis patients as rifampin was not stable in human lung macrophages, while Mycobacterium tuberculosis was able to survive in human lung macrophages. Rifampin was incorporated into submicron particles of PLGAs using the emulsion solvent diffusion method. The use of rifampin 50 mg in every batch resulted in the submicron-sized particles of 220 nm, PDI 0:12, zeta potential 21 mV and EE 37%. In the batch using rifampin 300 mg, resulted the submicron-sized particles of 410 nm, PDI 0:22, zeta potential 14 mV and EE 40%. The surface of the particles was visualized by SEM and hydrodynamic size compared to TEM. It was known that particle is spherical with a smaller diameter than the hydrodynamic size. TEM measurement revealed the size of particles with PVA was 208 nm.

Keywords: characterization, PLGA, rifampin, hydrodynamic-size, TEM, %EE.


9 | P a g e


LIQUID BATH SOAP FORMULATION AND ANTIBACTERIAL ACTIVITY TEST AGAINST Staphylococcus aureus OF KECOMBRANG (Etlingera elatior (Jack) R.M.Sm.) FLOS EXTRACTS

Lilis Handrayani1, Ratih Aryani1*, Indra1

1Pharmacy Study Programme, Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada Tasikmalaya

*E-mail : [email protected]

Abstract

The formulation of kecombrang flos extract (Etlingera elatior (Jack) R.M.Sm.) liquid bath soap has been established. The objective of this research was to formulate liquid bath soap of kecombrang flos extract (Etlingera elatior (Jack) R.M.Sm.) and to test its antibacterial activity to Staphylococcus aureus. Kecombrang flos extract was extracted by maceration method using 96% ethanol, and followed by minimum inhibitory concentration (MIC) test using hole method. Concentration variation of kecombrang flos extract was conducted as F1 (6%), F2 (8%), and F3 (10%). The formula of liquid bath soap of kecombrang flos extract was evaluated using several examinations such as organoleptic, pH, viscosity, density, foaming stability, antibacterial activity test, irritation test and hedonic test. The result shows the liquid bath soap of kecombrang flos extract F1, F2 and F3 can inhibit the growth of Staphylococcus aureus. Based on statistical test using SPSS 21 (for trial) ANOVA method continued by LSD shows that F0 (negative control), F1, F2, F3 and positive control (triclosan 2.5%) have difference meaningful result with significance value < 0.05.

Keywords: Kecombrang flos extract, Etlingera elatior, liquid bath soap, antibacterial, Staphylococcus aureus.


10 | P a g e




THE INFLUENCE OF EGGPLANT PEEL EXTRACT (Solanum melongena L.) AS ANTIOXIDANT IN LOTION DOSAGE FORM

Sholichah Rohmani1*

1Universitas Sebelas Maret Solo, Indonesia


Abstract

The purpose of this research is to formulate lotion containing extract of eggplant peel

as an effective and safe antioxidant lotion. Previous research showed that the anthocyanin in peel eggplant has antioxidant activity against free radical that cause aging on the skin. Extract of eggplant peel was obtained by maceration process using ethanol 70%. The extract was added in various concentrations in lotion formulation i.e. 0%, 0.5%, 1%, 2% and 3% based on the antioxidant activity that was determined using DPPH method. The produced lotions were characterized for the antioxidant activity, stability and physical properties including organoleptic, viscosity, pH and panellist acceptability. The result showed that antioxidant activity rated weekly and decreasing viscosity from week 0 to 8, pH of 5.62-6.93 was observed. Formulae I was the most acceptable lotion by pannelist. Keywords: peel eggplant, lotion, antioxidant.


11 | P a g e


OPTIMIZING COMBINATION OF SAMBILOTO HERBAL WATER FRACTION AND SALAM LEAF WATER FRACTION AS ANTI-INFLAMMATION

Raymond Harris Mustafa1*, Lannie Hadisoewignyo1, Martha Ervina1, Lisa

Soegianto1, Wahyu Dewi Tamayanti1

1Faculty of Pharmacy, Widya Mandala Surabaya Catholic University Surabaya, Indonesia


Abstract

Sambiloto herbs (Andrographis paniculata Nees) and salam leaves (Syzygium

polyanthum), which are effective to reduce blood sugar level with different mechanism, have been suggested to produce a synergy as antioxidant and anti-inflammatory agent, hence the optimum combination formula is remained to be elaborated. By reducing blood sugar level and showing antioxidant, and anti-inflammatory activity, both plants were hypothesized of its ability for ameliorating diabetes mellitus complexicity. This study aimed to discover the optimal combination formula of sambiloto herbs (Andrographis paniculata Nees) and salam leaves (Syzygium polyanthum) water fraction to produce anti-inflammation effect. Carrageenan was used to induced the inflammatory condition. Optimization was conducted by factorial design utilising 2 factors and 2 levels. Both factors, sambiloto herbs (Andrographis paniculata Nees) and salam leaves (Syzygium polyanthum) in the range of low level 1:10 and high level 10:1 with the used doses were 100 mg/kg BW at low level and 300 mg/kg BW for high level. The observed parameters were anti inflammation potential percent and edema rate. Conclusively, this study proposed that optimum combination formula of sambiloto herbal : salam leaves water fraction = 1.14 : 9.87 with 234 mg dose. The combination formula was theoretically resulted the optimum anti inflammation potential percent of 45.68%, and ER of 38.39% compared with other combination formulas.

Keywords: salam leaf, sambiloto herbal, water fraction, anti inflammation, factorial design.

12 | P a g e



OP B001 PHARMACOLOGY AND MICROBIOLOGY

THE POTENTIAL OF THE BLACK RICE BRAN EXTRACT AS ANTIDIABETIC AGENT

Arifah Sri Wahyuni1*, Rima Munawwaroh1, Esthi Utaminingsih1, Novita Sari1

1Faculty of Pharmacy University Muhammadiyah Surakarta Surakarta, Indonesia

*Email: [email protected]

Abstract

Natural hypoglycaemic compounds may be attractive alternatives to synthetic drugs or

reinforcements to currently used treatments. Black rice bran is one of natural compound that containing anthocianins. The major anthocyanins component isolated from black rice bran is cyanidin 3-glucoside. In this experiment, fiveteen male rat were randomly allocated into 5 equal groups. They were induced by alloxan (150mg/kgbw, i.p) except Group I). If the blood glucose levels reach 200 mg/dL, they were given aquadest (Group II), black rice bran extract (BBE) respectively 50, 100 and 200 mg/kg.bw (Group III-V). Blood glucose level was analyzed at 4, 7, 10 days treatment. Insulin level was determined enzyme linked immunosorbent assay (ELISA). The result showed that baseline levels of blood glucose were statistically not different among the fivegroups (p>0.05). By the end of experiment BBE dose 50 mg/kgbw did not reduce blood glucose levels after 10 days of treatment (p>0.05). BBE dose of 100 and 200 mg/kgbw decreased blood glucose levels. The level of blood glucose after administered 10 days by BBE 200 mg/kgbw was 131.33 ± 8.08 mg/dL. The results showed that insulin levels in diabetic rats increased by administration of the extract at dose 200 mg/kg b.w was 15,20±9,5ng/mL. It can be concluded that BBE can be therapeutically helpful as antidiabetic agents Keywords: cyanidin 3-glucoside, black rice bran extract, antidiabetic.


13 | P a g e

PP B002 PHARMACOLOGY AND MICROBIOLOGY

ANTIOXIDANT ACTIVITIES OF NANOEMULSION CONTAINING EXTRACT OF SAMBILOTO (Andrographis paniculata (Burm F.) Ness.) AND MENIRAN

(Phyllanthus niruriL.)IN ALLOXAN- INDUCED DIABETIC RATS

Erindyah R Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Da’i1, Doni Wibowo1

1 Faculty of Pharmacy, Universitas Muhammadiyah Surakarta Surakarta, Indonesia


Abstract

Combination of an oral antidiabetic with antioxidant is potential to treat disease and

the complications of diabetes mellitus. The aim of this study was to investigate the antioxidant activities of nanoemulsion containing extract of sambiloto (Andrographis paniculata (Burm F.) Ness.) and meniran (Phyllanthus niruri, L.) (NESM) in alloxan-induced diabetic rats. Rats were divided into 6 groups that were orally administered by nanoemulsion base (NE), vitamin E (100 mg/kg), NESM (100, 200 and 400 mg/kg) and combination of extract of sambiloto and meniran (ESM) (400 mg/kg) for 14 days. Blood was withdrawn on day 0, 1, and 14, and malondialdehyde (MDA) level was determined using UV-Vis spectrophotometer. The result showed that increasing dose of NESM reduced plasma MDA levels compared to NE, vitamin E and ESM groups. It can be concluded that NESM possess antioxidant activities. Keywords: nanoemulsion, Andrographis paniculata, Phyllanthus niruri, malondialdehyde (MDA), antioxidant.

14 | P a g e




ACUTE TOXICITY STUDY OF NANOEMULSION CONTAINING SAMBILOTO (Andrographis

paniculata) LEAVES AND MENIRAN (Phyllanthus niruri) HERBS EXTRACT IN WISTAR RATS

Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Da’i1, Febby Lovita Sari1

1Faculty of Pharmacy, Universitas Muhammadiyah Surakarta Surakarta, Indonesia

*Email: [email protected]

Abstract

Andrographis paniculata leaves (AL) and Phyllanthus niruri herbs (PH) have been

proven scientifically to have antidiabetic and antioxidant activities. Nanoemulsion system has been developed containing of both AL and PH extracts, using Tween 80 and polyethylene glycol as surfactant and cosurfactant, respectively. The present study is to investigate the acute toxicity of the nanoemulsion on Wistar rats. Five groups of rats were orally treated with nanoemulsion doses of 31.25, 125, 500, 2000 mg/kg and control. General behavior, adverse effects and mortality were observed for up to 14 days. Oral administration of nanoemulsion at the highest dose of 2000 mg/kg resulted in no mortalities or evidence of adverse effects, indicated that nanoemulsion is nontoxic with a LD50 higher than 2000mg/kg. Throughout 14 days of the treatment, there was no significant change in behavior and body weight of rats in both treatment and control groups. Histopalogical study showed that there were congestion in the liver and inflammation in the kidney by the highest dose of 2000 mg/kg. In addition, the pancreas and gastric of rats in the highest dose group showed a significant change compared to the control group. Keywords: acute toxicity, nanoemulsion, A. paniculata, P. niruri, herbal extract.


15 | P a g e


ANTI-INFLAMMATORY ACTIVITY TEST OF CHRISTMAS PALM (Adonidia merrillii (Becc.) Becc.) SEED EXTRACT IN MALE WISTAR RATS (Rattus norvegicus)

Herlina1*, Fitrya1, Fithri Najma A1, Rahmawati Dwi Shafarina1

1Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Sriwijaya University South Sumatera, Indonesia

*Email : [email protected]

Abstract

Christmas palm (Adonidia merrillii (Becc.) Becc.) contains flavonoids, tannins,

triterpenoids, and steroids. Antioxidant activity of christmas palm has been investigated. Based on research, antioxidant has a potential as an anti-inflammatory agent. Christmas palm seed extract anti-inflammatory activity test with rat paw edema method which induced by carragenaan has been done. Ethanolic, ethyl acetate, and n-hexane extract of christmas palm were divided into three dose groups, 200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW. Negative control was CMC Na in distilled water and positive control was 0.82 mg/200 g BW diclofenac Na. Thirty three Wistar male rats as test subjects were divided into 11 groups (1 negative control group, 1 positive control group, and 9 treatment groups). Anti-inflammatory activity was tested by measuring the volume of rat paw edema using plethysmometer. The test results showed the average of inhibition percentage of the ethanolic, ethyl acetate, and n-hexane extracts were 46.05%; 29.95%; and 34.63% respectively. This shows that the ethanolic extract has better inflammation inhibitory activity than ethyl acetate and n-hexane extracts. Non-parametric statistical Mann Whitney test of 800 mg/kg BW ethanolic extract at 30’ and 60’ showed that both were not significantly different (p>0.05) to diclofenac Na. Christmas palm ethyl acetate extract has anti-inflammatory activity far below diclofenac Na, but its effect was constant until 150’. n-hexane extract of 800 mg/kg BW at 60’ showed anti-inflammatory effect that was almost equivalent to diclofenac Na (p>0.05). Christmas palm seed extract ED50 has been calculated based on the average of edema inhibition percentage in rats. ED50 of christmas palm ethanolic extract is 640.42 mg/kg BW. Keywords: Adonidia merrillii (Becc.) Becc., anti-inflammatory, rat paw edema, diclofenac Na.


16 | P a g e




THE EFFECT OF GIVING GLUCOMANNAN PORANG TUBER (Amorphophallus oncophyllus Prain ex Hook. F.) ON SGPT AND SGOT LEVELS OF MALE WISTAR RATS

BLOOD INDUCED BY PARACETAMOL

Intan Martha Cahyani1*, Bekti Nugraheni1 1Sekolah Tinggi Ilmu Farmasi “YAYASAN PHARMASI”

Semarang, Indonesia


Abstract Indonesia is a country with a high prevalence of liver disease. Liver is important for

survival and plays a role in every metabolic function of the body. One of the causes of liver disorder is because of drugs. One of the drugs that cause liver damage is paracetamol. Glucomannan is a major component of the porang tuber that serves as a soluble fiber. Glucomannan is thought to have hepatotherapy effects as a potential antioxidant. The aim of this study is to determine the effect of glucomannan porang tuber (Amorphophallus oncophyllus Prain ex Hook. F.) with graded doses on the blood levels of SGPT and SGOT wistar male rats induced by paracetamol dose of 1,638 g/kg BB. This research was experimental study. The treatment in this study was porang glucomannan with a dose of 25 mg / kg, 50 mg / kg and 100 mg / kg. The Population of the study was white male Wistar rats aged 3-4 months with a weight of 180g - 250g, and healthy. The result of the analysis showed that glucomannan Porang (Amorphophallus oncophyllus Prain ex Hook. F.) has an effect as hepatotherapy. Giving glucomannan porang at a dose of 50 mg / kg rat has an effect to decrease blood levels of SGPT and SGOT wistar male rats induced by paracetamol. Keywords: Porang tuber (Amorphophallus oncophyllus Prain ex Hook. F.), SGPT, SGOT.



17 | P a g e


THE PROFILE OF MDA (malondialdehyde) LEVEL IN RATS GIVEN EXTRACT OF THYMI HERBS (Thymus vulgaris [L.])

Ken Inayati Latifa1*, Tanti Azizah Sujono1, Ika T. Dian Kusumowati1



Abstract

Oxidative stress occurs because of an imbalance between oxidants and antioxidants

which are then potentially cause damage to cells and reportedly play an important role in the process of liver damage. Free radicals can increase lipid peroxidation, which will then be decomposed into malondialdehyde (MDA) in the blood. MDA is a marker of cell damage caused by free radicals. Extract of Thymi contains chemical compounds such as terpenoids, flavonoids, aglycone, and phenolic acids. The content of flavonoid compounds contained in herbal Thymi can serve as an antidote to the radical. The purpose of this study is to determine the effect of herbal extracts Thymi in rats MDA levels. The study used 20 rats were divided into 4 groups. Group I was a normal control group was given distilled water of 2.5 ml/kg, while group II-IV were given Thymi herbal extract for 5 days with a dose of 50, 100, and 150 mg/KgBW, respectively. Blood was drawn for MDA content measurement using spectrophotometer at a wavelength of 530 nm. The results showed that herbal extracts Thymi can reduce levels of MDA in groups II, III, and IV. Meanwhile, there was also a decrease of MDA level in grup 1. So in this study decreased levels of MDA which occurs in group II, III, and IV can not be said as a result of administration of herbal extracts Thymi due to a decrease in MDA levels also occurred in group I were only given distilled water. Keywords: Thymus vulgaris, MDA (malondialdehyde).

18 | P a g e




PRE-CLINICALSTUDY OF Cr (III) BASED HYPOGLICEMIC SUPPLEMENT IN-TYPE 2 DIABETIC RATS

Kun Sri Budiasih1*, Kartika Ratna Pertiwi1

1Faculty of Mathematics and Natural Sciences, Yogyakarta State University Yogyakarta, Indonesia

*E-mail: [email protected] [email protected]

Abstract

The chromium (III)- amino acid complex based hypoglicemic agent was investigated

on nicotinamide-streptozotocin induced diabetic Wistar rats. The rats were divided into 7 groups each consist of 4 animals. Three groups are control (+) with chromium picolinate (CrPic), control (-) diabetic group (DM), and control non diabetic (non DM). Furthermore, three groups were examined on the effect of Cr-AA[Cr(µ-OH)(glu)(OH)2]2·6H2O at dose of (50, 150 and 300 µg/day). In addition, the last group was studied on the effect of control group by glibenclamide. The blood glucose levels were measured before and after treatment. The results show that supplementation of Cr(III)-complex in 8 weeks decreased the blood glucose level in the range of 46.446 – 79.593 %. Keywords: hypoglicemic, chromium (III), amino acid, complexes, nicotinamide-streptozotocin, diabetic rats.


19 | P a g e


SCREENING ANTIBACTERIAL POTENCY OF ENDOPHYTIC FUNGI METABOLITE OF MANGOSTEEN (Garciniamangostana L.) LEAF

Lisa Soegianto1*, Martha Ervina1, Kevin Widjaja1, Angela Violita1

1Faculty of Pharmacy, Widya Mandala Catholic University Surabaya Surabaya, Indonesia


Abstract

Mangosteen (Garcinia mangostana L.) is a tropical plant which its fruit peel is widely

used as an antioxidant, anti-diarrhea, anti-inflammatory, antitumor, and as an antibacterial. The previous study found the antibacterial activities of extract and metabolites of endophytic fungi of mangosteen rind. This research was aimed to explore utilization of mangosteen leaf and to screen antibacterial potency of the mangosteen leaf endophytic fungi metabolites. Endophytic fungi were isolated from leaf of mangosteen in the Malt Extract Agar (MEA) medium in order to get 2 colonies of endophytic fungi. Screening of potential antibacterial metabolites was assessed using diffusion method and bioautography, obtained results of the antibacterial activity against Escherichia coli (Ec) and Staphylococcus aureus (Sa) of the metabolites endophytic fungi of leaf. Macroscopic and microscopic characteristics from fungi isolate which has antibacterial potency, was observed and fermented into Potatoes Dextrose Yeast (PDY) medium for 14 days. At day 14, biomass and supernatant were separated and carried out separation by liquid-liquid extraction. The supernatant and biomass were fractionated using n-hexane, ethyl acetate, and water. Each fraction was eluated to several mobile phase and tested its antibacterial activity against Ec and Sa. The result showed that there is a potential antibacterial activity of endophytic fungi metabolites leaf ED2 against Sa. Bioautography result was observed that the compound has antibacterial activity and is supposed as flavonoid compounds. It was supposed that endophytic fungi ED2 was a group of Trichoderma. Keywords: mangosteen (Garcinia mangostana L.), endophytic fungi, antibacterial activity, Escherichia coli, Staphylococcus aureus.

20 | P a g e




ACTIVITIES OF THE COMBINED EXTRACTS OF TEMPUYUNG (Sonchus arvensis) AND BLACK CUMIN (NIGELLA SATIVA) AGAINST XANTHINE OXIDASE INHIBITION

ON HYPERURICEMIC MICE

Muhtadi1*, Nurcahyanti Wahyuningtyas1, Andi Suhendi1, Septi Heryani1



Abstract

The combination extracts of tempuyung (Sonchus arvensis) and black cumin (Nigella

sativa) can decrease uric acid levels on the previous result research. However, the mechanism of decreasing uric acid levels was unknown certainly. This study aimed to determine the inhibitory effect of xanthine oxidase by the combination extracts of black cumin and tempuyung on hyperuricemic mice. Hyperuricemic mice were induced by 250 mg/kgBW potassium oxonate was given one hour before treatment. The mice were divided into 3 groups, group I was given 10 mg/kgBW allopurinol (positive control), group II was given 0.5 mL/20gBW aquadest (negative control) and group III was given the combination extracts of black cumin-tempuyung with dose 200 mg/kgBW during 4 days administration. The supernatant of liver was taken and measured of xanthine oxidase levels by spectrophotometer UV at 290 nm. The data of xanthine oxidase activity were analyzed by Kruskal-Wallis and Mann-Whitney method. Xanthine oxidase activity of the combination extracts of blackcumin-tempuyung was 4.54±0.9 U/mg, very significantly than control negative was 8.00±0.22 U/mg (p<0.05). Inhibition of xanthine oxidase by the combination extracts of black cumin-tempuyung was 43.26±11.29%, lower than allopurinol was 90.19±0.36%.

Keywords: Hyperuricemic, xanthine oxidase inhibition, Sonchus arvensis, Nigella sativa.


21 | P a g e


ANTIBACTERIAL ACTIVITY OF COMBINATION OF CHLORAMPHENICOL AND ETHANOLIC EXTRACT OF PACAR AIR (Impatiens balsamina) LEAVES AGAINST

Escherichia coli AND Shigella sonnei

Ratna Yuliani1*, Agung Cokro Prabowo1, Yeni Maisyah1



Abstract

Chloramphenicol, a broad spectrum antibiotic, is used to treat several bacterial

infections. However, it has several side effects such as headache, rash, diarrhea, nausea, vomiting, bone marrow suppression, and aplastic anemia. Combining antibiotic with another antibacterial agent may decrease the dose thus the side effects. This study aimed to investigate the antibacterial activity of chloramphenicol combined with ethanolic extract of pacar air (Impatiens balsamina) leaves against Escherichia coli and Shigella sonnei. Chloramphenicol (30 µg), extract (2500 µg), combination of chloramphenicol and extract (22.5 µg + 625 µg and 15 µg + 1250 µg), dimethylsulfoxide, and water for injection were tested for antibacterial activity against Escherichia coli and Shigella sonnei using well diffusion method. Data was analyzed using Mann-Whitney test.The results showed that only chloramphenicol (22.5 µg) combined with extract (625 µg) has inhibition zone diameter, which was not significantly different from chloramphenicol alone (30 µg) when it tested against E. coli. This result indicated that combination of the antibiotic in lower concentration and extract can achieve the same antibacterial activity as antibiotic alone in higher concentration. Keywords: antibacterial, chloramphenicol, Impatiens balsamina, combination.

22 | P a g e




ANTIHYPERCHOLESTEROLEMIC EFFECT OF Murbei (Morus alba L.) LEAVES AND ITS COMBINATION WITH SIMVASTATIN IN RATS INDUCED BY PROPYLTIOURACIL

AND HIGH FAT DIET

Tanti Azizah Sujono1*, Haryoto1, Ratna Kartikasari1, Laily Ieda Quntari1


*E-mail:[email protected]

Abstract

Empirically Murbei leaves are used to treat diseases such as hypercholesterolemia.

Murbei leaves contain quercetin 3- (6-malonylglucoside) and rutin which shows a strong inhibitory effect on LDL oxidation. This study aimed to verify the effect of the ethanol extract of murbei leaves in lowering total cholesterol in hyperlipidemic rats and the effect of combination extract murbei with simvastatin to the hypocholesterolemic effect of simvastatin. Twenty four Wistar male rats were divided into 6 groups randomly, group I-VI were given high fat diet and (propyltiouracil) PTU to induce hypercholesterolemia. Group I as positive control was treated simvastatin 3.6 mg/kgbw, group II as a negative control was given CMC Na, group III-V were given ethanol extract of murbei leaves orally at a dose of 0.4; 0.6; and 0.8 g/kgbw respectively, group VI were treated a combination of murbei extract 0.4 g/kg with simvastatin. Total cholesterol was measured using a spectrophotometer visible (λ = 500 nm) with CHOD-PAP method as many as 3 times, i.e on day 0 (baseline), 14 days after induced hypercholesterolemia with PTU and high-fat-diet and 14 days after treatment of murbei extract with still be given PTU and a high-fat diet. The results showed that murbei leaves extract dose 0.4; 0.6 and 0.8 g/kg were able to reduce total cholesterol with percent decrease in cholesterol levels by 32.94 ± 10.07; 40.17 ± 4.61 and 45.71 ± 4.27% respectively. Ethanol extract of murbei 0.4 g/kg can increase hypocholesterolemic effect of simvastatin when used in combination (p< 0.05).

Keywords : Murbei (Morus alba L), antihypercholesterolemic, propyltiouracil, high fat diet.


23 | P a g e


ANALGESIC EFFECT OF ETHANOLIC EXTRACT RED DRAGON FRUIT ( Hylocereus polyrhizus Cortex) PEEL ON MALE MICE SWISS WEBSTER WITH

WRITHING REFLEX METHOD

Westi Fajrin Bayu Nugrahaini1*, Tanti Azizah Sujono1


*E-mail:[email protected]

Abstract

Red Dragon fruit (Hylocereus polyrhizus) is one of the plants that grow in Indonesia.

Peel of fruit (Hylocereus polyrhizus) contain oligosacharida and Betasianin. Oligosacharida showed activity as prebiotic, which can lower the resistance to acidic conditions in the stomach, meanwhile betasianin can protect the cells from damage caused by free radical. This research aims to prove whether peel of Dragon fruit has activity as analgesic in mice. Twenty five mice were divided into 5 groups. group I was given Na CMC 1% (negative control), group II was treated paracetamol 65 mg/kgBW (positive control), group III, IV, and V were given ethanolic extracts of dragon fruit peel at dose 0.25, 0.5, and 1 g/kgBW respectively. Twenty minutes later, all mice were induced pain by 0,1 ml intraperitoneal acetic acid 1% and the cumulative of writhing reflect of mice were calculated for one hour. Then percent inhibition of writhing were analyzed by Kruskal Wallis test and continued by Mann-Whitney with 95% confidence level. The results showed that ethanolic extracts of dragon fruit peel showed analgesic effect. It can decrease the number abdominal constrictions and also increased the percentage inhibition of writhing at dose of 0.25, 0.5, and 1 g/kgBW with percent of inhibition 42,76 ± 2,04; 1.42 ± 49,32; and 61,38 ± 1,37% respectively. Keywords: Hylocereus polyrhizus, Dragon fruit peel, analgesic, writhing reflect

24 | P a g e




EFFECTS OF TURMERIC ETHANOLIC EXTRACT ON TRIMETHYLTIN-INDUCED OXIDATIVE STRESS ON SPRAGUEY DAWLEY RATS

Sapto Yuliani1*, Mustofa Mustofa2, Ginus Partadiredja2

1Faculty of Pharmacy, Universitas Ahmad Dahlan 2Faculty of Medicine, Universitas Gadjah Mada

Yogyakarta, Indonesia


Abstract

Oxidative stress, an imbalance between free radicals and the antioxidant system, is known to contribute to the pathogenesis of the development of dementia. Ethanolic extract from turmeric (Curcuma longa L.) containing the curcumin constituent has been reported to produce antioxidant effects. This study aims to determine the effect of turmeric extract on markers of oxidative stress in Spraguey Dawley rat induced by (trimethyltin) TMT. Thirty six adult male Sprague-Dawley rats (195-215 g) were divided randomly into six groups consisting of 6 rats for each group. The rats were divided randomly into six groups, i.e.: N group, which served as a normal control group; T group, which was given intra-peritoneal injection of TMT chloride; T-Cit group, which was treated with oral citicoline and TMT chloride injection; and three T-TE groups, which were treated with three different dosages of oral turmeric rhizome extract, as well as TMT chloride.The turmeric rhizome extract and citicoline solutions were given at day 1 up to day 28 of experiment, whereas the TMT chloride injection given as a single dose of 8 mg /kg bw was administered at day 8 of experiment. At day 36 the blood were taken for plasma MDA level determination. Afterthat all rats were sacrificed and the cerebral hemisphere were then dissected out from the skull. The left cerebral hemispheres were used for biochemical observation i.e. MDA and GSH level, activities of SOD, catalase (CAT) and GPx. The turmeric extract dose of 200 mg/kg bw could prevent oxidative stress induced by TMT through the decrease of levels of plasma and brain MDA and increased the activities of SOD, CAT, GPx, and the level of GSH of brain. These effects seem to be comparable to those of citicoline. Keywords: Curcuma longa L., trimethyltin, oxidative stress.


25 | P a g e


ANTIDIABETIC ACTIVITY OF NANOEMULSION CONTAINING EXTRACT OF SAMBILOTO (Andrographis paniculata (BURM F.) NESS.) AND MENIRAN

(Phyllanthus niruri L.) IN ALLOXAN-INDUCED DIABETIC RATS

Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Da’i1, Fajar Kholikul Amri1



Abstract

Preparation of nanoemulsion containing combination of extract of A. Paniculataand P.

Niruri (NESM) has been successfully developed. The present study was carried out to investigate the antidiabetic activity of the nanoemulsion preparation in alloxan-induced diabetic rats.Rats were divided into 6 groups that were given oral administration of nanoemulsion base (NE), glibenclamide (5 mg/kg), NESM (100, 200 and 400 mg/kg) and combination of extract of sambiloto and meniran (ESM) (400 mg/kg) for 14 days. The hypoglycemic effect was measured by blood glucose level. Oral administration of the NESM at a dose of 100, 200, and 400 mg/kg daily for 14 days showed a significant (P<0.05) decrease in blood glucose level as compared with the diabetic rats. The results indicated that the nanoemulsion preparation possesses significant antidiabetic activity. Keywords: nanoemulsion, Andrographis paniculata (Burm F.) Ness., Phyllanthus niruri, L., antidiabetic.

26 | P a g e



OP C001 CLINICAL AND COMMUNITY PHARMACY

OVERVIEW ABOUT ANTIBIOTIC’S PRESCRIBING IN URINARY TRACT INFECTION

ROEMANI SEMARANG HOSPITAL’S INPATIENTS

Hening Pratiwi1*

1Studied Program of Pharmacy UNSOED Purwokerto, Indonesia


Abstract

Inappropriate antibiotics prescribing in Urinary Tract Infection (UTI) can lead antibiotic’s resistance. Therefore, hospitals should have a formulary as a reference for providing medical services to the patients. This study aims to determine the types of antibiotics that prescribed for UTI treatment on January until November 2009 and determine the level of antibiotics prescribing conformity with the Roemani Semarang hospital’s formulary and WHO 2001 guidelines. This study used a non-analytical descriptive design and retrospectively. The samples were 73 patients. This study includes the pattern of antibiotic prescribing in UTI patients and conformity with 2009 hospital formulary and 2001 WHO guidelines. The results showed that antibiotics are widely used cefotaxime (cephalosporins) 14 cases (24%), levofloxacin (quinolones) 11 cases (18%), and ceftriaxone (cephalosporins) 10 cases (17%). The combination that widely prescribed are cephalosporins combination with quinolones 3 cases (21%), cephalosporin combination with other cephalosporins 3 cases (21%), combination of cephalosporin with an aminoglycoside 2 cases (14%), there are 68 prescriptions (93%) suitable with hospital formulary, and 5 prescriptions (7%) not listed on the formulary of Roemani hospital 2009. The UTI antibiotic monotherapy in women, men, and children do not exist in accordance with the WHO guidelines 2001. Keywords: Urinary Tract Infections, antibiotics, hospital formulary, RS. Roemani Semarang.


27 | P a g e

PP C002 CLINICAL AND COMMUNITY PHARMACY

RATIONALITY TREATMENT OF ANTIBIOTICS FOR TREATMENT OF DIARRHEA IN ADULT PATIENTS IN THE INPATIENT INSTALLATION OF HOSPITAL “X”

SURAKARTA IN 2014

Ida Ayu Pebrina1*, Suharsono1, Suprapto1



Abstract

Diarrhea is still a public health problem in developing countries such as Indonesia, due

to its morbidity and mortality are still high. Antibiotics are used to attack microbial infection and kill or inhibit the growth of bacteria. The purpose of this study is to determine the rationality of the use of antibiotics in adult patients with diarrhea in the inpatient installation of hospital "X" Surakarta in 2014. The study used non-experimental method. The study used 46 medical records of patients using purposive sampling technique. The instrument that used is the data collection sheets and the medical records of diarrhea patient as the material. From the data that has been captured and processed, the rationality of antibiotic therapy for the treatment of diarrhea include: precise indication of the percentage of 100 % , right drug as much as 97.82%, right dosage as much as 97,82% , proper frequency and duration of drug administration as much as 78.26%. Keywords: antibiotics, diarrhea, adult patient.

28 | P a g e



OP C003 CLINICAL AND COMMUNITY PHARMACY

CLINICAL IMPROVEMENT AFTER CEPHALOSPORINE THERAPY ON CHILDREN WITH TYPHOID FEVER

Rasmaya Niruri1*, N.P. Yulia Purnami1, Julius D.Tansale2,3, I.B.Eka Erlangga3

1Department of Pharmacy, Faculty Math and Science, Udayana University 2Petri Bali, Bali

3Puri Raharja Hospital, Denpasar, Bali, Indonesia


Abstract

The aim of this research is to evaluate clinical recovery time on children with typhoid fever (TF) after receiving cephalosporin. This cross-sectional research was conducted in Puri Raharja Hospital. Data of 12 TF clinical features was collected from all pediatric patients with TF on the period of January 1st, 2013 to March 31st, 2014, who received the same antibacterial medicine during hospitalization. A patient, who had fever clearance and showed general well-being, was considered having a good clinical-response from TF. A patient was discharged after showing a good clinical therapeutic response. Seventy nine from 89 subjects were prescribed with cefotaxime, and the rest got ceftriaxone (9 patients) and cefixime (1 child). Length of stay in the hospital (median [time range] in days) were 5 [4-10] with cefotaxime; 4 [4-7] with ceftriaxone; and 7 [7] with cefixime. All of 89 children showed good clinical-response to the medicines. The majority of children were free from TF symptoms when they were discharged, but not from weakness- fatigue. Keywords: clinical improvement, children, typhoid.



29 | P a g e


TRANSAMINITIS ASSOCIATED WITH HIGH DOSE METHOTREXATE AND

6-MERCAPTOPURIN IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA

Rasmaya Niruri1*, K. Trisna Komalasari1, Ketut Ariawati2

1Department of Pharmacy, Faculty of Math and Science, Udayana University, Bali 2, Division of Hematology-Oncology, Department of Pediatric, Sanglah Hospital, Bali

Bali, Indonesia


Abstract

The objective of this research was to evaluate the level of AST and AST before and after consolidation phase of Indonesian Protocol (2006) Chemotherapy on acute lymphoblastic leukemia (ALL).This cross sectional research was conducted in Sanglah Hopital. All hospitalized children (0-12 tahun) in the period of January 2012 – May 2014, who got high dose methotreaxate (HD MTX) and 6-mercaptopurin (6-MP) and had normal AST-ALT level before receiving HD -MTX- and 6-MP, were included. Thirteen of 39 patients who met the criteria was obtained, but only ten patients, who had complete AST-ALT data on week 8th, 10th, and 12th of consolidation phase. From 10 children who had the complete data, four of them (who didn’t receive Ursodeoxycholic acid (UDCA)) had the highest transaminase enzyme level at week 12th and the rest 6 patients showed declining AST-ALT level after receiving UDCA.

Keywords: transaminitis, HD-MTX, 6-MP, children, ALL.


30 | P a g e




TREATMENT AND COST ANALYSIS OF DIARRHEA PATIENTS OF INPATIENT INSTALLATION OF RSUD dr. MOEWARDI SURAKARTA BY BPJS PROGRAM IN 2014

Saktya Ayu Donna P1*, Suharsono1, Suprapto1



Abstract

Diarrhea or gastroenteritis is still a major disease in developing countries that can lead to death. This research was aimed to analyse the cost and treatment of gastroenteritis’s patients of inpatient installation using BPJS program at RSUD Dr. Moewardi in 2014. This research used descriptive, non-experimental, and retrospective method by collecting data from the medical report of patients. There are 28 cases fulfil inclusive criterion. Cost was analysed including average total cost, prescription, medical stay, laboratory, visit, action and health tools and administration cost. The result showed that (a) Average total cost of diarrhea therapy every class treatment: VIP class is Rp.3.239.007 ± Rp.1.914.830, class I is Rp.1.522.475 ± Rp.0, class II is Rp.2.964.331 ± Rp.368.155; class III Rp.2.043.954 ± Rp.1.716.563. (b) class treatment with most cheap is class III is Rp.2.043.954 ± Rp.1.716.563. Stay care diarrhea’s patient of BPJS at RSUD Dr. Moewardi year 2014 uses medicine groups are rehydration liquid (100%) RL is 17 cases (65.38%) and asering is 9 cases (34.62%). Antibiotic groups are 81 patients (81%), adsorben group is 75 cases (75.0%), antiulcer kidney group is 16 cases (19.75%), antiemetic is 9 cases (11.11%), analgetic-antipeuritic 9 cases (11.11%) supplement food is 9 cases (11.11%) and probiotic is 1 case (1.23%). Keywords: cost analysis, RSUD dr. Moewardi, diarrhea.


31 | P a g e

OP D001 PHARMACEUTICAL CHEMISTRY

ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF PARKIA SEED AND POD (Parkia speciosa Hassk.) BY DPPH (1,1-DIPHENYL-2- PICRYLHYDRAZYL) METHOD

Indri Kusuma Dewi1*, Agus Winarso1

1Department of Jamu Poltekkes Kemenkes Surakarta Klaten, Indonesia


Abstract

Parkia speciosa is one of plants that usually used for food materials. Seed and pod of

Parkia speciosa contain flavonoid that act as antioxidant. The aim of this study was to examine antioxidant activity at ethyl acetate extract of seed and pod Parkia speciosa tha were expressed by Inhibitor Concentration 50 (IC50). Testing of antioxidant activity was done by DPPH method (1,1-dipheny-2-picrylhydrazyl) using spectrophotometer UV-Vis at wavelength 518 nm. The result showed that ethyl acetate extract of the seed of Parkia speciosa had low antioxidant activity with the IC50 value of 274.702 ppm, while ethyl acetate extract of the pod Parkia speciosa did not show any antioxidant effect with the IC50 value of 685.857 ppm. Keywords: Parkia speciosa, seed, pod, antioxidant, DPPH.

32 | P a g e



OP D002 PHARMACEUTICAL CHEMISTRY

A CLICK-TYPE COUPLING REACTION BETWEEN THIOAMIDES AND SULFONYL AZIDES AS A VERSATILE APPROACH TO GENERATE NEW PHARMACOLOGICALLY

ACTIVE COMPOUNDS

Muhammad Aswad1, Junya Chiba1*, Yasumaru Hatanaka1, Takenori Tomohiro1 1Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama

Sugitani, Toyama, Japan


Abstract

Several click-type reactions have been developed and applied to biological conditions, however, the ligations generally need some catalysts or additives for their practical use. We recently reported a chemoselective reaction between thioamides and sulfonyl azides to afford sulfonyl amidines in the absence of any activation additives.1 The reaction proceeded by mixing the thioamide and sulfonyl azide at room temperature in various solvents, and water exhibited the highest performance with respect to efficiency. The characteristics of amidines within the product framework are polar and hydrophilic, so that we applied this reaction for the simple derivatization of sugars, such as nojirimycin, to develop novel inhibitors for glucosidases. A cyclic thioamide derivative of nojirimycin could be synthesized from gluconolactone derivatives.2By coupling of the thioamide with several sulfonyl azides, the chemoselective ligation successfully afforded the corresponding amidine compounds, which are the potential candidates as novel glycosidase inhibitors.


33 | P a g e

PP D003 PHARMACEUTICAL CHEMISTRY

ANTIBACTERIAL COMPOUND PRODUCED BY A SOIL BACTERIA ISOLATED FROM RIZHOSPHERE OF Zingiber officinale

Nanik Sulistyani1,2*, Yosi Bayu Murti3, Jaka Widada4, Mustofa5

1Faculty of Pharmacy, Universitas Ahmad Dahlan 2Doctoral Program, Faculty of Medicine, Universitas Gadjah Mada

3Faculty of Pharmacy, Universitas Gadjah Mada 4Departement of Microbiology, Faculty of Agriculture, Universitas Gadjah Mada

5Departement of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada Yogyakarta, Indonesia


Abstract

Isolation of bacteria from Zingiber officinale rizosphere in Magelang, Central Java,

Indonesia has been carried out and got many isolates in the previous study. One of them is isolate J4 having potencial activity as antibiotic producer. This study aims to identify the antibiotic produced by isolate J4. Research was performed with examining the antibacterial activity of ethyl acetate extracts of culture broth and their fractions using cup-plate method as well as bioautography. The active fractions were then analysed using IR Spectroscopy. Identification of selected bacteria was based on the 16S rRNA gene sequence and the active fraction was characterized by LC-TOF-MS to identify the molecular mass of compounds contained in the fractions. Result showed the isolate J4 revealed antibacterial activity of both extracts and their fractions. The active fraction is the chloroform-methanol (7:3) fraction. According to its IR spectra, there was detected OH and CH stretching vibration as well as carbonyl stretching. Based on the LC-TOF-MS, the active fraction contains 3 compounds with molecular mass of 270, 274 and 404. The BLAST of 16S rRNA sequence revealed that isolate J4 is Burkholderia sp. Keywords: antibiotic, isolate J4, soil bacteria, Burkholderia sp.

34 | P a g e



OP E001 MOLECULAR BIOLOGY

CYTOTOXIC ACTIVITY OF POLAR, SEMIPOLAR, AND NON POLAR FRACTION OF ETHANOL EXTRACT OF SALA PLANTS LEAVES (Cynometra ramiflora Linn.)

AGAINTS WiDr CELL

Haryoto1*, Anis N. Irjayanti1, Tanti Azizah Sujono1, Muhtadi1, Andi Suhendi1



Abstract

Previous study reported that ethanolic extract of Sala plants leaves (Cynometra

ramiflora Linn.) had cytotoxic activity against WiDr cells with IC50 of 6.37 μg/mL. This study aims to determine the cytotoxic activity of polar, semipolar, and nonpolar fraction of ethanolic extract of Sala plants leaves (Cynometra ramiflora Linn.) against WiDr cells, their IC50s, and compounds contained in each fraction of ethanol extract of Sala plants leaves. Fractionation was performed using Vacuum Liquid Chromatography method with silica G60 as stationary phase and n-hexane:ethylacetate (8:2, 7,5:2,5, 7:3,6:4, and 3:7) and ethanol as mobile phase. Compounds contained in each fraction was analysed using thin layer chromatography method with silica GF254 as stationary phase and mobile phase n-hexane:ethylacetate (7:3). Cytotoxic activity assay was performed using MTT assay method. Polar fraction of ethanol extract of Sala plants leaves has cytotoxic activity against WiDr cells with IC50 value of 231.953 μg/mL. Semipolar and nonpolar fractions do not show cytotoxic activity against WiDr cells. Doxorubicin was used as positive control and obtained IC50 of 1.721 μg/mL. The polar fraction contains of flavonoids, phenolics and alkaloids, while the non-polar and semipolar fractions contain phenolic compounds and alkaloids. Keywords: Cytotoxic, fractionation, MTT assay, Cynometra ramiflora, WiDrcell, IC50.


35 | P a g e


CHALCONES DERIVATIVE WITH BROMO SUBSTITUENT INDUCES APOPTOSIS IN HeLa CELLS

Retno Arianingrum1*, Indyah Sulistyo Arty1

1Department of Chemistry Education, Universitas Negeri Yogyakarta Yogyakarta, Indonesia


[email protected]

Abstract

Chalcones, a group of aromatic enones, is known have a variety of biological properties, including anticancer. We have synthesized a chalcone derivate 1-(4’-bromophenyl) -3-(4-hydroxy-3-methoxyphenyl)-2-propene-1-on by aldol condensation reaction. The aim of the present study was to investigate the anticancer potency of the compound in HeLa cervical cancer cells by observing cytotoxic and apoptotic effect. The cytotoxic properties were determined using MTT assay and apoptosis induction was carried out using flowcytometry. The

result indicated that the compound has cytotoxic effect on HeLa cells with IC50 of 53 M and was able to induce apoptosis. Hence, 1-(4’-bromophenyl) -3-(4-hydroxy-3-methoxyphenyl)-2-propen-1-on is potential to be developed as anticancer agent for cervix cancer by inducing apoptosis. However, the molecular mechanisms need to be explored further. Keywords: chalcones derivate with Bromo substituens, cytotoxic, apoptosis, HeLa.



36 | P a g e




SUB-CLONING OF ads GENE INTO pETDUET1_cyp FOR CO-EXPRESSION IN ESCHERICHIA COLI

Imam A. Wicaksono1, Tresna Lestari2*, Evi U. Ulfa3, Catur Riyani1, Elfahmi1

1Institut Teknologi Bandung 2STIKes Bakti Tunas Husada Tasikmalaya

3Universitas Jember Indonesia


Abstract

CYP71AV1 and ADS are two enzymes involved in artemisinin biosynthesis. In this

research, sub-cloning of cyp71av1 and ads in pETDUET1 (pETDUET1_cyp/ads) has been done. The result of transformation has been confirmed by migration, restriction and sequencing analysis. Overproduction of CYP71AV1 and ADS was done at temperature 37 °C using 0.5 mM IPTG induction. The protein produced mostly formed as inclusion bodies, therefore the optimization of overproduction condition is still needed. Keywords: CYP71AV1, ADS, pETDUET1, Sub-cloning.


37 | P a g e

CV OF SPEAKER

Jackie Y. Ying received her B.E. and Ph.D. from The Cooper Union and Princeton University, respectively. She joined the faculty at Massachusetts Institute of Technology in 1992, where she was Professor of Chemical Engineering until 2005. She has been the Founding Executive Director of the Institute of Bioengineering and Nanotechnology in Singapore since 2003. For her research on nanostructured materials, Prof. Ying has been recognized with the American Ceramic Society Ross C. Purdy Award, David and Lucile Packard Fellowship, Office of Naval Research Young Investigator Award, National Science Foundation Young Investigator Award, Camille Dreyfus Teacher-Scholar Award, American Chemical Society Faculty Fellowship

Award in Solid-State Chemistry, Technology Review’s Inaugural TR100 Young Innovator Award, American Institute of Chemical Engineers (AIChE) Allan P. Colburn Award, Singapore National Institute of Chemistry-BASF Award in Materials Chemistry, Wall Street Journal Asia’s Asian Innovation Silver Award, International Union of Biochemistry and Molecular Biology Jubilee Medal, Materials Research Society Fellowship, Royal Society of Chemistry Fellowship, American Institute for Medical and Biological Engineering Fellowship, and Crown Prince Grand Prize in the Brunei Creative, Innovative Product and Technological Advancement (CIPTA) Award. Prof. Ying was elected a World Economic Forum Young Global Leader, and a member of the German National Academy of Sciences, Leopoldina. She was named one of the “One Hundred Engineers of the Modern Era” by AIChE in its Centennial Celebration. She was selected by The Muslim 500 in 2012, 2013 and 2014 to be one of the world’s 500 most influential muslims. She was selected as an Inaugural Inductee for the Singapore Women’s Hall of Fame in 2014. She is the Editor-in-Chief of Nano Today, which has an impact factor of 15.000.

38 | P a g e



CV OF SPEAKER

Full name: Takenori Tomohiro, Ph.D. Current position: associate professor, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama Education/Career: 1980: University of Tsukuba (~1984) 1984: Graduate School of Pure and Applied Sciences, University of Tsukuba (~1986) 1986: National Chemical Laboratory for Industry (reorganized to "National Institute of Advanced Industrial Science and Technology" : ~ 2002)

1992: University of Oxford (UK) as a postdoctoral researcher (~1994) 2002: Université Louis Pasteur (France) as a visiting professor 2002: Toyama Medical and Pharmaceutical University (reorganized to "University of Toyama") Current publications: 1. Tomohiro, T., Morimoto, S., Shima, T., Chiba,

J., Hatanaka, Y. An isotope-coded fluorogenic cross-linker for high-performance target identification based on photoaffinity labeling. Angew. Chem. Int. Ed. 2014, 53, 13502-13505.Tomohiro, T., Yamamoto, A., Tatsumi, Y., Hatanaka, Y. [3-(Trifluoromethyl)-3H-diazirin-3-yl]coumarin as a carbene-generating photocross-linker with masked fluorogenic beacon. Chem. Commun. 2013, 49, 11551-11553.

2. Aswad, M., Chiba, J., Tomohiro, T., Hatanaka, Y. Coupling reaction of thioamides with sulfonyl azides: an efficient catalyst-free click-type ligation under mild conditions. Chem. Commun. 2013, 49, 10242-10244.

3. Tomohiro, T., Inoguchi, H., Masuda, S., Hatanaka, Y. Affinity-based fluorogenic labeling of ATP-binding proteins with sequential photoactivatable cross-linkers. Bioorg. Med. Chem. Lett. 2013, 23, 5605- 5608.

4. Morimoto, S., Tomohiro, T., Maruyama, N., Hatanaka, Y. Photoaffinity casting of a coumarin flag for rapid identification of

ligand-binding sites within protein. Chem. Commun. 2013, 49, 1811-1813.

5. Tomohiro, T., Kato, K., Masuda, S., Kishi, H., Hatanaka, Y. Photochemical Construction of Coumarin Fluorophore on Affinity-Anchored Protein, Bioconjugate Chem. 2011, 22, 315-318.

6. Masuda, S., Tomohiro, T., Hatanaka Y. Rapidly photoactivatable ATP probes for specific labeling of tropomyosin within the actomyosin protein complex, Bioorg. Med. Chem. Lett. 2011, 21, 2252-2254.

7. Kashiwayama, Y., Tomohiro, T., Narita, K., Suzumura, M., Glumoff, T., Hiltunen, J. K., Van Veldhoven, P. P., Hatanaka, Y., Imanaka, T. Identification of a substrate-binding site in a peroxisomal b-oxidation enzyme by photoaffinity labeling with a novel palmitoyl derivative. J. Biol. Chem. 2010, 285, 26315-26325.

8. Review: Tomohiro, T., Hatanaka, Y. Diazirine-based multifunctional photo-probes for affinity-based elucidation of protein-ligand interaction. Heterocycles 2014, 89, 2697-2727.

Patent: 8 patents and 4 patent-pending


39 | P a g e

CV OF SPEAKER

Wangsa Tirta Ismaya, PhD Education: Universitas Padjadjaran 1993-1998 Chemistry / Biochemistry · Bachelor of Sciences Indonesia · Bandung University of Groningen 2002-2011 Biophysical Chemistry / Biochemistry · PhD Netherlands · Groningen

Research Experience: Research Scientist

April 2013-present: Dexa Laboratories of Biomolecular Sciences · Biopharmaceuticals Technology · Recombinant Protein Therapeutics Research Group Indonesia · Cikarang Postdoctoral Fellow Feb 2012-Jan 2013 Georgia Institute of Technology · Institute for Bioengineering and Bioscience · Bioengineering of Natural Products USA · Atlanta April 2009-Jan 2012 Universiteit Utrecht · Department of Biochemistry and Cell Biology (DBC) · Bifunctional Protein Netherlands · Utrecht Research Scholar Feb 2008-Feb 2009: University of Groningen · Groningen Biomolecular Sciences and Biotechnology Institute (GBB) · Protein X-ray Crystallography Netherlands · Groningen

Teaching/Research Assistant Bandung Institute of Technology · Pusat Antar Universitas - Bioteknologi · Biokimia Indonesia · Bandung Publication: 1. Ismaya, W. T., Rozeboom, H. J., Weijn, A., Mes, J. J., Fusetti, F., Wichers, H. J., & Dijkstra, B. W.

(2011). Crystal structure of Agaricus bisporus mushroom tyrosinase: identity of the tetramer subunits and interaction with tropolone. Biochemistry, 50(24), 5477-5486.

2. Ismaya, W. T., Rozeboom, H. J., Schurink, M., Boeriu, C. G., Wichers, H., & Dijkstra, B. W. (2011). Crystallization and preliminary X-ray crystallographic analysis of tyrosinase from the mushroom Agaricus bisporus. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 67(5), 575-578.

3. Ismaya, W. T., Hasan, K., Kardi, I., Zainuri, A., Rahmawaty, R. I., Permanahadi, S., ... & Soemitro, S. (2013). Chemical modification of Saccharomycopsis fibuligera R64 α-Amylase to improve its stability against thermal, chelator, and proteolytic inactivation. Applied biochemistry and biotechnology, 170(1), 44-57.

4. Natalia, D., Vidilaseris, K., Ismaya, W. T., Puspasari, F., Prawira, I., Hasan, K., ... & Soemitro, S. (2015). Effect of introducing a disulphide bond between the A and C domains on the activity and stability of Saccharomycopsis fibuligera R64 α-amylase. Journal of biotechnology, 195, 8-14.

5. Etc

40 | P a g e



LIST OF COMMITTEES

Host Organizer : Pharmacy Faculty University of Muhammadiyah Surakarta

Steering Committee : Rectorate board of UMS, Dean of Faculty of Pharmacy UMS

Organizing Committee Chair : Dr. Azis Saifudin, Apt

Email : [email protected] Mobile : +62 82135670601

Secretary : 1. Dr. Anita Sukmawati, Apt 2. Alfian Mahardika F., Apt 3. Rahmadhani Tyas, Apt

Treasurer : 1. Ika Trisharyanti, M.Pharm., Apt

2. Dyah Susilawati 3. Sri Rahayu

Conference programme

: 1. Dr. Erindyah Retno Wikantyasning, Apt 2. Arini Fadhilah, Apt 3. Juwita Rahmawati, Apt 4. Aan Wahyu Widodo 5. Muchamad Zein Arif 6. Lusiana Putri 7. Mahardika Putri Bestari 8. Sita Sofiana 9. Chinthia Devientasari

Registration and Administration

1. Hidayah Karuniawati., M.Sc., Apt 2. Titis Putri I, Apt 3. Cita Hanif M, Apt 4. Avanilla Fany S., Apt 5. Fa’is Ayu Febrina 6. Umul Salamah 7. Siti Susilowati 8. Anisa Widyaratna 9. Vindhy Mulya Gustina

Moderator : 1. Dr. Zakky Cholisoh, Apt (plenary session) 2. Dr. Anita Sukmawati, Apt 3. Dr. Haryoto, M.Sc 4. Dr. Maryati., Apt 5. RatnaYuliani, M.Biotech,St 6. Broto Santoso, M.Sc., Apt 7. Gunawan Setiyadi., Apt 8. Suprapto, M.Sc., Apt

Scientific committee : 1. Dr. Erindyah R Wikantyasning, Apt 2. Tanti Azizah, M.Sc., Apt 3. Dr. M. Da’i, Apt 4. Dr. Muhtadi, M.Si. 5. Dr. Anita Sukmawati., Apt 6. Dr. Zakky Cholisoh, Apt 7. Dr. Azis Saifudin, Apt 8. Dr. Maryati, Apt



41 | P a g e

9. Dra. Nurul Mutmainah, Apt 10. Arifah S Wahyuni, M.Sc., Apt 11. Dedi Hanwar, M.Si., Apt 12. Suprapto, M.Sc., Apt 13. Rima Munawaroh, M.Sc., Apt 14. RatnaYuliani, M.Biotech.St 15. Agus Purnomo, M.Biotech

Proceeding : 1. Tanti Azizah, M.Sc., Apt

2. Normaidah, S.Farm. 3. Ana Amalia 4. Ilmi Nurul Fatkhi 5. Devy Anwar Zhelsiana 6. Martha Nadhira 7. Pratiwi Widowati

Equipment and Room : 1. Suprapto, M.Sc., Apt

2. Rahmat Partono 3. Y Daru Prabowo 4. Hadi Cahyo, Apt 5. Subhan Rosyad Abidi 6. Akhmad Nafarin 7. Tifan Adji Hutama 8. Andriyanto Saputro

Transport and Accomodation

1. Abdul Shomad 2. Zaenal Mustakim 3. Wisnu Adi Nugroho 4. Ongki Arbiyanto 5. Muhammad Arif Maulia Husna 6. Agung Beny Santosa

Photograph and Documentation

1. Al Wathony 2. Awang Pribudi 3. Iwan Setiawan, Apt 4. Bagas Aji Kusuma 5. Muhammad Nur Prasetyo

42 | P a g e



MAP OF FACULTY OF PHARMACY

Garden

Garden

STO

RE

STO

RE

N


43 | P a g e

LIST OF TAXI AND HOTELS

LIST OF TAXI

Kosti Solo Ph. +62-0271-856300 Solo Sentral Ph. +62-0271-728728 Bengawan Ph. +62-0271-734666 Gelora Ph. +62-0271-7004999 Sakura Ph. +62-0271-644194 Angkasa Ph. +62-0271-781315 LIST OF HOTELS

Stars Hotel Alamat

*****

Kusuma Sahid Prince Hotel Solo

Jl. Sugiyopranoto 20 Solo 57111 Telp: +62 (271) 646356 Fax: +62 (271) 644788

*****

Hotel Sahid Jaya Solo

Jl. Gajah Mada 82, Solo 57132 Telp: +62 (271) 644144 Fax: +62 (271) 644133

*****

The Royal Surakarta Heritage

Jl. Slamet Riyadi No. 6 Solo 57111 Telp: +62 (271) 666111 Fax: +62 (271) 666530 Email: reservation @theroyalsurakartaheritagesolo.com

*****

Lorin Solo Hotel

Jalan Adisucipto No 47 Solo 57174 Telp: +62 (271) 724500 Fax: +62 (271) 724400

****

Solo Paragon Hotel & Residences

Jl. Dr. Soetomo Solo 57125 Telp: +62 (271) 7655888 Fax: +62 (271) 7655700 Email: [email protected]

****

The Sunan Hotel Solo

Jl. Ahmad Yani 40 Solo 57143 Telp: +62 (271) 731312 Fax: +62 (271) 738677

****

Hotel Novotel Solo

Jl. Slamet Riyadi 272 Solo 57131 Telp: +62 (271) 724555 Fax: +62 (271) 724666 Email: [email protected]

http://www.kusumasahid.com/



http://www.sahidjayasolo.com/

http://www.sahidjayasolo.com/

http://theroyalsurakartaheritagesolo.com/



http://lorinhotel.com/index.php/our-hotels/loringroups/lorin-hotel-solo

http://lorinhotel.com/index.php/our-hotels/loringroups/lorin-hotel-solo

http://www.soloparagonhotel.com/



http://thesunanhotelsolo.com/

http://thesunanhotelsolo.com/

http://www.novotel.com/gb/hotel-0545-novotel-solo/index.shtml

http://www.novotel.com/gb/hotel-0545-novotel-solo/index.shtml

44 | P a g e



Stars Hotel Alamat

***

Hotel Indah Palace Solo

Jl. Veteran 284 Solo Telp: +62 (271) 711011 Fax: +62 (271) 724368 Email: [email protected]

***

Syariah Hotel Solo

Jalan Adisucipto No 47 Solo 57175 Telp: +62 (271) 711000 Fax: +62 (271) 736969

Al Madina Syariah Hotel Solo

Jl. Duwet Raya No. 37 Pabelan Kartasura Solo Telp: +62 (271) 765 2975 Email: [email protected]

http://hotelindahpalace.com/welcome.php?lokasi=Solo



http://www.syariahhotelsolo.com/

http://www.syariahhotelsolo.com/

http://www.hotelsyariahsolo.com/



INTERNATIONAL CONFERENCE - UMS

Documents

Transcript of INTERNATIONAL CONFERENCE - UMS