INTEGRAL UNIVERSITY LUCKNOW

SYLLABUS

of

B.TECH. BIOTECHNOLOGY

2016-17

SYLLABUS & EVALUATION SCHEME FOR

B. TECH. BIOTECHNOLOGY

STUDY & EVALUATION SCHEME

B. TECH. Biotechnology

(w.e.f.- July 2016)

2nd

Year III Semester

Sl.

No

Course

Category

Subject

Code

Name of the Subject

Periods Evaluation Scheme

Subject

Total Sessional (CA) End Sem.

Exam.

(ESE) L T P C CT TA Total

1. DC BE-201 Biochemistry 3 1 0 4 25 15 40 60 100

2. DC BE-202 Microbiology 2 1 0 3 25 15 40 60 100

3. ESA ME -221 Fluid Flow and Solid

Handling 2 1 0 3 25 15 40 60 100

4. ESA MT-203 Numerical Analysis &

Statistical Analysis 2 1 0 3 25 15 40 60 100

5. DE

CS-228 Concepts of web

development

2 1 0 3 25 15 40 60 100 BE-205 Plant Physiology

BE-206 Animal Physiology

6. ESA ES -202/

CS-203

Disaster Management/

Cyber Law & Information

Security

2 1 0 3 25 15 30 60 100

7. HM

BM-226 Human Values &

Professional Ethics 3 0 0 ** 0 0 0 50* 50*

8. DC BE -203 Biochemistry lab 0 0 6 3 30 30 60 40 100

9. DC BE -204 Microbiology lab 0 0 6 3 30 30 60 40 100

10. ESA ME -223 Fluid Mechanics lab 0 0 4 2 30 30 60 40 100

Total 16 6 16 27 240 180 410 480 900

L – Lecture T – Tutorial P – Practical C – Credits CT – Class Test TA – Teacher

Assessment

Sessional Total (CA) = Class Test + Teacher Assessment

Subject Total = Sessional Total (CA) + End Semester Examination (ESE)

BS – Basic Sciences DC – Departmental Core

HM – Humanities OE – Open Elective

DE – Departmental Elective ESA – Engineering Sciences & Arts (Foundation Course &

Engineering Courses)

BIOCHEMISTRY

BE-201

(w.e.f.- July 2016)

L T P C

3 1 0 4 Unit I [08] Role of water in biological processes: Solvent, solute, solutions, water and its properties,

hydrogen ion concentration, Buffers system. General structure, function and classification of

carbohydrates (mono, di and poly sacharride.), lipids (Fatty acids, Phospholipids, Glycolipids,

Waxes), proteins (primary, secondary and tertiary) and nucleic acid (Purines, pyrimidines,

nucleosides, nucleotides.). Structure and functions of vitamins and hormones.

Unit II [08] Carbohydrates Metabolism: Introduction, Glycolysis and its regulation, Oxidative and

reductive decarboxylation, TCA cycle, Amphibolic role of TCA cycle, Anaplerotic reactions,

glyoxylate cycle, pentose phosphate pathway, Oxidative phosphorylation (energy

metabolism). Biosynthesis of polysaccharides: Photosynthesis, gluconeogenesis.

Unit III [08] Lipid metabolism: Oxidation of fatty acids- saturated and unsaturated, beta, gamma, omega

oxidations and regulations. Biosynthesis of fatty acids, cholesterol and phospholipids.

Unit IV [08] Amino acid metabolism: Biosynthesis and oxidation of glutamate, serine and aspartate family

amino acids and their regulation. Urea cycle, Nitrogen fixation, (Biological, nif gene).

Unit V [08] Nucleic acid metabolism: Biosynthesis of purines & pyrimidines by de novo & salvage

pathway and their regulations. Biodegradation of purines and pyrimidines and regulation.

Books recommended: 1. Hames and Hooper; Instant notes on biochemistry; Viba Books PVT. Ltd.

2. Nelson, Cox; Lehninger's, Principle Biochemistry; Macmillan Worth Publication

3. L.Stryer; Biochemistry;

4. Voet and Voet; Biochemistry; Freeman WH and Co., NewYork

5. Mathews et al; Biochemistry; Pearson education.

6. S.C Rastogi; Biochemistry; Tata Macgraw.

MICROBIOLOGY

BE-202

(w.e.f. July 2016)

L T P C

2 1 0 3

UNIT-1 [8]

Introduction to microbiology: definition, history of microbiology (from1684 to modern era of

microbiology), Biogenesis and Abiogenesis, Koch’s postulates. Basics knowledge of

microorganisms: Bacteria, fungi, viruses, actinomycetes, protozoa etc. prokaryotic and

eukaryotic cells, Structure of bacterial cell wall: External and Internal, Gram positive and Gram

negative bacteria.

UNIT-2 [8]

Principles of microscopy: light microscope (magnification and resolution), bright field

microscope, phase contrast, dark field, TEM and SEM microscopy, Gram staining and endospore

staining.

UNIT-3 [8]

Microbial nutrition and growth: microbial requirements of macro and micronutrients, microbial

media and types, microbial isolation techniques: dilution, pour plate and streak plate, Microbial

growth curve and growth measurements, pure culture, starter culture, cultural characteristics of

bacteria, classical, nutrional and modern bacterial taxonomy, Phylogenetic tree of bacteria.

UNIT-4 [8]

Extremophiles: thermophiles, psychrophiles, acidophiles, alkaliphiles etc. Nitrogen fixing

bacteria, Coliform bacteria: Microbiological examination of fecal contaminated water, Food and

water borne infectious diseases: (botulism and salmonellosis), Control of microorganisms:

Physical (autocalve, filteration, radiation and pasteurization) and chemical methods (antibiotics,

alcohol and halogens).

BOOKS RECOMMENDED:

1. Prescott, Harley and Klevin: Microbiology; 2nd

ed

2. Brock, Micheal and Clark: Microbiology of Microorganisms; 12th

edition

3. Introduction to Microbiology: Pelczar

FLUID FLOW AND SOLID HANDLING

ME -221

(w.e.f.- July 2016)

L T P C

2 1 0 3

Unit I [08]

Solid and their handling Properties of solids, screening, industrial screening equipment, determination of particle size,

screen analysis, size reduction of solids, stages of reduction, operating variables, intermediate

and fine size reduction, power requirement and mechanism, Power driven machines: Crushers,

grinders and conveyers.

Unit II [08]

Fluid flow Properties of fluids, Fluid Statics: Fluid pressure, Hydrostatic Law and Manometers, Fluid

dynamics: Euler’s equation of motion, Bernoulli’s equation from Euler’s equation, Applications

of Bernoulli’s equation. Minor losses in pipe flow.

Unit III [08]

Flow measurements Construction, working and application of flow measuring devices: Orifice meter, Nozzle flow

meter, Venturimeter, Rota meter and Pitot tube. Simple calculation on them.

Unit IV [08]

Pumping and compressing Centrifugal Pump: Classifications, working, Introduction to vector diagram and work done by

impeller. Reciprocating pump: Reciprocating pump theory, Slip and coefficient of discharges,

Indicator diagram, Effect and acceleration, Work saved by fitting air vessels. Introduction of

positive displacement pump, blowers and fluidization.

Books recommended: 1. Introduction of Fluid Mech'anics by Robert W. Fox and Slan T. Mcdonald, John urley & sons,

Ny. Fourth Ed.

2. Unit Operation in Chemical Engg., Mccable Smith., 5th edition

3. Bedger W.L. and Bancharo J. T. "Introduction to Chemical Engineering" Tata McGraw

publication House.

4. Hydraulic Machines: Jagdish Lal, Metropolitan Book Co.

5. Hydraulics and Fluid Mechanics: Modi and Seth, Standard Book House

Numerical and Statistical Analysis

MT-203

(w.e.f. July 2016)

L T P C

2 1 0 3

Unit-I [7]

Errors in numerical computations, numerical solution of algebraic and transcendental

equations by Bisection method, Iteration method, Regula false method, Newton-

Raphson’s method.

Unit-II [9]

Finite differences: forward and backward differences, properties of operators, factorial

polynomials, Interpolation: Newton Gregory forward and backward interpolation formula.

Interpolation formula for unequal intervals, divided differences formula, central

interpolation formula: Bessel, Stirling and Laplace Everett's interpolation formulae.

Unit-III [7]

Numerical differentiation, Numerical integration by Trapezoidal rule, Simpson’s 1/3 rule,

3/8 rule, Boole's rule, Weddle’s rule.

UNIT-IV [8]

Probability: Classical and axiomatic definition of probability. Addition and Multiplication

theorem of probability. Conditional probability. Probability distributions: Binomial

distribution, Poisson distribution and Normal distribution.

Unit -V [9]

Testing of Hypothesis: Statistical hypothesis, null and alternative hypothesis, simple and

composite hypothesis, critical region, type I and II error, power of a test. Test of significance

based on t, F and chi-square distribution. Basic concepts of Simple random sampling and

Stratified random sampling. Analysis of variance (ANOVA): One-way and two way

classification.

Reference Books:

1. Text book of Numerical Analysis: H.C. Saxena (S. Chand Publication)

2. Numerical Analysis: S. S., Shastri

3. Numerical Analysis: B.S. Grewal.

4. Numerical Analysis: A.R. Vashistha.

5. Q.S Ahmad, V. Ismail and S.A Khan: Biostatistics, laxmi Publications Pvt.Ltd.

6. S.C. Gupta and VK. Kapoor: Fundamental of Mathematical Statistics, Sultan Chand

publication.

DISASTER MANAGEMENT

ES-202 (w.e.f.- July 2016)

L T P C

2 1 0 3 Objective:

The objective of this course is to familiarize the student with basic management principles

relating to disaster management and mitigation techniques.

Units I [8] Concept of disaster management. Types of disaster and their impact: Natural and Man- Made,

Like- Earthquakes, Floods, Tsunami, Droughts, Cyclones, Avalanches, Forest Fire, Terrorism

related Disaster etc. Assessment of human and Economic losses.

Units II [8] Impact of extensive Industrialization. Impact of Global Warming and Environmental

Degradation. National and global disasters.

Units III [8] National policy for disaster Management, Elementary Knowledge of the Disaster Management

Act 2005. Types of Responses: Central, State, District Level, Peoples Community participation

in Disaster Management. Post-Disaster Management and Rehabilitation measures.

Unit IV [8] Capacity Building for meeting disasters. Long term measures for preventions of disasters.

Mitigation technique/strategies: Early warning systems, Data sharing at national and

international level.

References: 1. Disaster management by Dr. V.K Sethi

2. The great Sumatra Earthquakes and Indian Ocean tsunami of December 2004- the effects of

main land India and in the Andaman and Nicobar Island, published by IIT Kanpur.

3. Environmental managaenet by Dr. Shakeel Ahmad

4. Hazards, disasters and your community, ministry of home affairs.

CYBER LAW AND INFORMATION SECURITY

CS-203 (w.e.f.- January 2017)

L T P C

2 1 0 3 Unit1 [8] Fundamentals of Cyber Law: Jurisprudence of Cyber Law, Object and Scope of the IT Act 2000,

Introduction to Indian Cyber Law, Unicitral Model Law, ISP Guideline. Intellectual property

issues and cyber space, Indian perspective, Overview of Intellectual property related legislation

in India, Patent, Copy Right, Trademark law, Law related to semiconductor layout & design.

Unit 2 [8] E - Commerce: Security Threats to E - Commerce, Virtual Organization, Business Transactions

on Web, E-Governance and EDI, Concepts in Electronics payment systems, E-Cash,

Credit/Debit Cards, E- Agreement, Legal recognition of electronic and digital records, E-

Commerce Issues of privacy, Wireless Computing- Security challenges in Mobile devices.

Digital Signatures - Technical issues, legal issues, Electronic Records, Digital Contracts,

Requirements of Digital Signature System.

Unit 3 [8] Investigation and Ethics: Cyber Crime, Cyber jurisdiction, Cyber crime and evidence act,

Treatment of different countries of cyber crime, Ethical issues in data and software privacy,

Plagiarism, Pornography, Tampering computer documents, Data privacy and protection, Domain

Name System, Software piracy, Issues in ethical hacking. Internet security treats: Hacking,

Cracking, Sneaking, Viruses, Trojan horse, Malicious Code & logic bombs. Introduction to

biometric security and its challenges, Finger prints. Cyber crime forensic: CASE STUDY in

Cyber Crime.

Unit 4 [8] Information security- Information Systems and its Importance, Role of Security in Internet and

Web Services, Principles of Information Security, Classification of Threats and attacks, Security

Challenges, Security Implication for organizations, Security services - Authentication,

Confidentiality, Integrity, Availability and other terms in Information Security, Information

Classification and their Roles. Introduction to Cryptography, Issues in Documents Security,

Keys: Public Key, Private Key, Firewalls, Basic Concepts of Network Security, Perimeters of

Network protection & Network attack, Need of Intrusion Monitoring and Detection.

References:

1. Harish Chander “Cyber Law and IT Protection” , PHI Publication, New Delhi

2. Merkov, Breithaupt “ Information Security”, Pearson Education

3. “Cyber Law in India” - Farooq Ahmad-Pioneer books.

4. K. K. Singh, Akansha Singh “Information Security and Cyber law”, Umesh Publication, Delhi

HUMAN VALUES & PROFESSIONAL ETHICS

BM-226

Maximum Marks: 50

External Assessment: 50

L T P C

3 0 0 0

Unit-1 [6]

Human Value Education: Understanding the need, basic guidelines, content and process for Value Education, Self

Exploration - Its content and process; ‘Natural Acceptance’ and Experiential Validation- as the mechanism for self

exploration, Continuous Happiness and Prosperity- A look at basic Human Aspirations, Right understanding,

Relationship and Physical Facilities- the basic requirements for fulfillment of aspirations of every human being with

their correct priority, Understanding Happiness and Prosperity correctly.

Unit-2 [6]

Introduction to Ethical Concept: Definition of industrial ethics and values, Ethical rules of industrial worker.

Values and Value Judgments. Moral Rights and Moral rules, Moral character and responsibilities. Privacy,

Confidentiality, Intellectual Property and the Law. Ethics as Law

Unit-3 [6]

Professional Responsibility: The basis and scope of Professional Responsibility, Professions and Norms of

Professional Conduct, Ethical Standards versus Profession, Culpable mistakes, the Autonomy of professions and

codes of ethics. Employee status and Professionalism. Central Professional Responsibilities of Engineers: The

emerging consensus on the Responsibility for safety among engineers, hazards and risks.

Unit-4 [6]

Engineers Ethics: Senses of 'Engineering Ethics' - variety of moral issues - types of inquiry - moral dilemmas -

moral autonomy - Kohlberg's theory - Gilligan's theory - consensus and controversy – Models of Professional Roles

- theories about right action - Self-interest - customs and religion - uses of ethical theories. Valuing Time – Co-

operation – Commitment.

Unit-5 [6]

Global Issues: A Glimpse of Life Stories: Life story of Prophet Mohammad, Mahatma Gandhi, Swami

Vivekanand, Marie Curie and Steve Jobs.

Multinational corporations - Environmental ethics - computer ethics - weapons development - engineers as

managers-consulting engineers-engineers as expert witnesses and advisors -moral leadership

Reference Readings:

Text Book

1. R R Gaur, R Sangal, G P Bagaria, 2009, A Foundation Course in Value Education.

2. Mike Martin and Roland Schinzinger, “Ethics in Engineering”, McGraw-Hill, New York 1996.

3. Govindarajan M, Natarajan S, Senthil Kumar V. S, “Engineering Ethics”, Prentice Hall of India, New Delhi, 2004.

Relevant CDs, Movies, Documentaries & Other Literature:

1. Value Education website, http://www.uptu.ac.in

2. Story of Stuff, http://www.storyofstuff.com

3. Al Gore, An Inconvenient Truth, Paramount Classics, USA

4. Charlie Chaplin, Modern Times, United Artists, USA

5. IIT Delhi, Modern Technology – the Untold Story

6. The Hundred, Michael Hart

BIOCHEMISTRY LAB

BE-203 (w.e.f.- July 2016)

L T P C

0 0 6 3 1. Estimation of carbohydrates

2. Estimation of proteins

3. Estimation of nucleic acids

4. Isoelectric precipitation

5. Separation of amino acids by paper chromatography

6. Extraction of lipids

7. Thin layer chromatography

8. Gel electrophoresis

9. Assay of enzyme activity and enzyme kinetics

Books recommended: 1. S.K Sawhney; Introductory Practical biochemistry; Narrosa Pub.

2. J. Jayaraman; Lab. Manual in Biochemistry; New Age Int. Pub.

3. Wilson and Walker; Practical Biochemistry; Cambridge publication. David T

Plummer; An introduction to practical biochemistry; Tata McGraw Hill.

MICROBIOLOGY LAB

BE-204 (w.e.f.- July 2016)

L T P C

0 0 6 3

1. Preparation of nutrient agar slants, plates and nutrient broth and their

sterilization. (Microwave Oven, Heating mantles, Fridge, Heating Oven, Tube

racks)

2. Inoculation of agar slants, agar plate and nutrient broth (Incubators, Water bath,

Laminar hood, dry heat sterilizer i.e. bead sterilizer)

3. Culture of microorganisms using various techniques. (Shakers i.e. Cooling and

Open shaker).

4. Simple and differential staining procedures, endospore staining, flageller

staining,

5. Cell wall staining, capsular staining, negative staining. (Moist chambers, spirit

lamps, slides, loops & microscopes, haemocytometer)

6. Bacterial colony counting. (Moist chambers, spirit lamps, slides, loops &

microscopes, haemocytometer)

9. Observation of different vegetative, capsular and spore forms of bacteria

&Fungus (under various microscopes).

10. Isolation of microbes from soil samples and determination of the number of

colony forming units. (UV spectrophotometer, Colony counters etc.)

11. Study of growth curve of E. coli

Books recommended: 1. Lab Manual in microbiology by P Gunasekaran (New Age Int. Pub.)

FLUID MECHANICS LAB

ME -223

(Say minimum 8 experiments out of the following) (w.e.f.- July 2016)

L T P C

0 0 4 2

1. To verify Bernoulli’s equation experimentally.

2. To calibrate a venturimeter meter and study the variation of coefficient of

discharge with Reynolds number.

3. To calibrate an orifice and study the variation of coefficient of discharge with

Reynolds number.

4. To study the velocity distribution in a pipe.

5. To study the transition, from laminar to turbulent flow and determine the lower

critical Reynolds number.

6. To study the variation of friction factor for turbulent flow in smooth commercial

pipes.

7. To verify impulse momentum equation experimentally and compare the force

exerted by the jet on different shapes of vanes. (flat, hemispherical).

8. To determine experimentally the metacentric height of a ship model.

9. To plot the flow net for a given model with the help of Heleshwaw apparatus.

10. To determine the coefficient of discharge of an orifice of a given shape. Also

determine the coefficient of velocity and coefficient of contraction of the orifice.

11. To calibrate a given v-notch or a rectangular notch and determine the

coefficient of discharge

Concepts of Web Development

CS-228

(w.e.f. July 2016)

L T P C

2 1 0 3

Unit 1

Introduction and Web Development Strategies

History of Web, Protocols governing Web, Creating Websites for individual and Corporate

World, Cyber Laws, Web Applications, Writing Web Projects, Identification of Objects, Target

Users, Web Team, Planning and Process Development. [6]

Unit 2

HTML/DHTML

Introduction, Objectives, Introduction to Universal Resource Identifier (URI) – Fragment

Identifiers and Relative URI’s, History of HTML, SGML, Structure of HTML/DHML

Document, Switching between opened Windows and browser (Container tag, Empty tag,

Attribute), Basic Tags of HTML, Heading tag, FONT tag and Attributes, Formatting Text,

Ordered List, Unordered List, Creating Links, Tables, Frames. [6]

Unit 3

CSS (Cascading Style Sheet)

Introduction, Syntax, Inclusion, Measurement Units, Colors, Backgrounds, Fonts, Text, Images,

Links, Tables, Borders, Margins, Lists, Padding, Cursors Outlines, Dimension, Scrollbars,

Introduction of XML. [7]

Unit 4

JavaScript

Overview, Syntax, Enabling, Placement, Variables, Operators, If…Else, Switch Case, While

Loop, For loop, For…In, Loop Control, Functions, Events, Cookies, Page Redirect, Dialog

Boxes, Void Key Word, Page Printing. [6]

Unit 5

PHP Hypertext Preprocessor (PHP)

Introduction of PHP, Common Uses of PHP, Characteristics of PHP, “Hello World” script in

PHP, Environment Setup, Syntax Overview, Variables, Constants, Operators, Decision Making,

Loop Arrays, String Web Concept. [7]

References: 1. Burdman, “Collaborative Web Development”, Addison Wesley.

2. Jeffrey C. Jackson, “Web Technologies – A Computer Science Perspective”, Pearson Education.

3. XML & Related Technologies by Atul Kahate, Pearson Education India.

4. PHP Reference: Beginner to Intermediate PHP5 By Mario Lurig

Plant Physiology

BE-205

(w.e.f. July 2016)

L T P C

2 1 0 3

UNIT I 8

Plant cells, tissues, and organs; cell organelles and their physiological functions; Structure and

physiological functions of cell walls; Growth and development in plants

UNIT II 8

Transport in plants: Movement of water, gases and nutrients; Cell to cell transport-Diffusion,

facilitated diffusion, active transport; Plant – water relations – Imbibition, water potential,

osmosis, plasmolysis; Long distance transport of water – Absorption, apoplast, symplast,

transpiration pull, root pressure and guttation; Transpiration – Opening and closing of stomata;

Uptake and translocation of mineral nutrients, Transport of food, phloem transport

UNIT III 8

Mineral nutrition: Essential minerals, macro and micronutrients and their role; Mineral

deficiency and their symptoms; Mineral toxicity; Elementary idea of Hydroponics as a method to

study mineral nutrition

UNIT IV 8

Role of growth regulators in plants-auxin, gibberellin, cytokinin, ethylene, ABA; Seed

dormancy; Vernalization; Phytochromes- mode of action; photoperiodism- short day, long day

and day neutral plants; photoperiodic induction; photoperiod and plant flowering

Reference Books

1. Plant Physiology by Lincoln Taiz and Eduardo Zeiger

2. Plant Physiology by Frank Salisbury and Cleon Ross

3. Text Book of Plant Physiology, Biochemistry, and Biotechnology by V.K. Varma and Mohit

Varma, S. Chand Ltd.

ANIMAL PHYSIOLOGY

BE-206

(w.e.f. July 2016)

L T P C

2 1 0 3

Unit I Tissues: Types of tissue. Epithelium- organization and types. The basement membrane. Bone and

cartilage. The extracellular matrix of epithelial and nonepithelial tissues. Elementary details of ECM

components. Blood and circulation - Composition and functions of blood. Separation of plasma and

serum. Plasma proteins in health and disease. Red blood cells- formation and destruction. The RBC

membrane- principal proteins (spectrin, ankyrin, glycophorins). Composition and functions of WBCs.

Blood coagulation- mechanism and regulation. Fibrinolysis. Anticoagulants. [8]

Unit II Cardiovascular and Respiratory System: Comparative anatomy of heart structure, myogenic

heart, specialized tissue, ECG – its principle and significance, cardiac cycle, heart as a pump, blood

pressure, neural and chemical regulation. Respiratory system - transport of gases, exchange of gases,

waste elimination, neural and chemical regulation of respiration. [8]

Unit III Digestive and Excretory system: Digestion, absorption, energy balance, BMR. Digestion and

absorption of carbohydrates, lipids, proteins and nucleic acids. Excretory system - Comparative

physiology of excretion, kidney, urine formation, urine concentration, waste elimination, micturition,

regulation of water balance, electrolyte balance. . [8]

Unit IV Neuromuscular system and Endocrinology: Structure of neuron. Propagation of action

potential: The neuromuscular junction. The acetylcholine receptor. Gross neuroanatomy of the brain and

spinal cord, central and peripheral nervous system. Structure of skeletal muscle. Sequence of events in

contraction and relaxation of muscle. Endocrine glands, basic mechanism of hormone action, hormones

and diseases. [8]

Books for Reference:

1. Lodish et al. Molecular Cell Biology 6th ed. 2007, WH Freeman

2. Murray et al. Harper’s Illustrated Biochemistry 27th ed. McGraw Hill 2006

3. Smith et al. Principles of Biochemistry. Mammalian Biochemistry. McGraw Hill 7th ed.

4. De Robertis and De Robertis. Cell and Molecular Biology.Lippincott 2001

5. Alberts et al. Molecular Biology of the cell 5th ed. Garland Sci. 2007.

6.Guyton and Hall.Textbook of Medical Physiology 12 ed 2011