Instructions for Use - Glyco Kit MB-LAC WGA

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The CARE product range is specifically optimized and certifiedfor all Bruker Daltonics systems.

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Instructions for Use

Glyco Kit MB-LAC WGAAs part of Bruker Daltonics’ CLINPROT™ system the Glyco Kits are especially designed forsophisticated glycoprotein studies and clinical proteomics research. Super-paramagnetic glyco-beads are functionalized with either boronic acids (BA) or lectins like concanavalin A (Con A),wheat germ agglutinin (WGA), Lens Culinaris Agglutinin (LCA), artocarpus integrifolia (AIA orJacalin). With a combination of different optimized beads, intelligent enrichment strategies forglycosylated peptides and proteins prior to mass spectrometry enable advanced glycomicanalyses. With the Bruker Daltonics’ mass spectrometry workflow, glycoproteins from complexsamples like human serum can be reliably and reproducibly captured and identified within adynamic range of eight orders of magnitudes. Besides glycoprotein identification andcharacterization as well as glyco-structure predictions, CLINPROT™ glyco-beads are suitable forsample fractionation prior to profiling analyses.

LC-MALDI

Identification ofglycoproteins

LC-MALDI

Identification ofN-glycosylation sites

PNGase F digestion

LC-MALDI

Structural characterizationof N-glycosylated peptides

Sample

Bruker Daltonics CLINPROT™ magnetic glyco-beadsSeparation on protein level

(e.g. ConA, LCA, WGA, AIA or BA)

Tryptic digestion

Bruker Daltonics CLINPROT™ magnetic glyco-beadsSeparation on peptide level

(ConA)

CLINPROTTM beadpurification

(e.g. C8, WCX, IMAC-Cu)

Glyco-Profiling

LC-MALDI

Identification ofglycoproteins

LC-MALDI

Identification ofN-glycosylation sites

PNGase F digestion

LC-MALDI

Structural characterizationof N-glycosylated peptides

Sample

Bruker Daltonics CLINPROT™ magnetic glyco-beadsSeparation on protein level

(e.g. ConA, LCA, WGA, AIA or BA)

Tryptic digestion

Bruker Daltonics CLINPROT™ magnetic glyco-beadsSeparation on peptide level

(ConA)

CLINPROTTM beadpurification

(e.g. C8, WCX, IMAC-Cu)

Glyco-Profiling

Fig. 1: Range of possible experimental strategies for the application of Bruker Daltonics’ CLINPROTTM

magnetic glyco-beads.

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Figure 1 exemplarily depictured possible experimental strategies to facilitate the identification ofglyco-proteins, to enable the detection of N-glycosylation sites, to support the structuralcharacterization of N-glycosylated peptides and to perform glyco-profiling studies in the clinicalcontext.

General Information

Glycosylation is the most common post-translational modification of proteins, playing a key role inprotein folding, stability, solubility, activity and molecular recognition. Altered glycosylation is knownto act as an indicator or effector in pathologic mechanisms with potential impact on diagnosis andtherapy of human diseases. Immunity to certain infectious diseases, prion diseases and cancercan all be associated with abnormal glycosylation patterns. As an example, increased branchingand sialylation of N-linked glycans on cancer cells are potential tumor markers in moleculardiagnostics.

Bruker Daltonics’ CLINPROT™ magnetic glyco-beads, specifically functionalized with lectins orboronic acids that bind to various structural motifs, facilitate the selective isolation of differentglycoproteins out of complex biological sources (Fig. 1). Bruker Daltonics has developed workflowscombining the specific pre-fractionation employing glyco-specific magnetic particles, e.g. ConA,WGA, LCA or boronic acids coated beads, and LC-MALDI analysis to identify glycoproteins, tolocate N-glycosylation sites and to get structural information about N-glycosylated peptides.

ManGlcNAc

FucGal

NeuAc

N-linked glycans

O-linked glycans

High-mannose type Hybrid type Complex type

GalNAc

Glycoprotein GlycoproteinGlycoprotein

Glycorotein Glycoprotein

C-linked glycans

Glycoprotein

MB-LACCon A

MB-LACWGA

MB-LACLCA

MB-LACAIA

MB-CovACboronic

ManManGlcNAcGlcNAc

FucFucGalGal

NeuAcNeuAc

N-linked glycans

O-linked glycans

High-mannose type Hybrid type Complex type

GalNAcGalNAc

GlycoproteinGlycoprotein GlycoproteinGlycoproteinGlycoproteinGlycoprotein

Glycorotein GlycoproteinGlycoroteinGlycorotein GlycoproteinGlycoprotein

C-linked glycans

GlycoproteinGlycoprotein

MB-LACCon A

MB-LACWGA

MB-LACLCA

MB-LACAIA

MB-CovACboronic

MB-LACCon A

MB-LACWGA

MB-LACLCA

MB-LACAIA

MB-CovACboronic

Fig. 2: Binding motifs of Bruker Daltonics’ CLINPROTTM magnetic glyco-beads. The different beadsrepresent overlapping but individual binding profiles.

Successful applications and reproducibility of Bruker Daltonics’ CLINPROTTM magnetic glyco-beads using model systems or complex samples like serum, plasma or urine were demonstrated invarious studies1,2,3. Further, long term stability and storage conditions were extensivelyinvestigated to assure the high quality of Bruker Daltonics’ CLINPROTTM magnetic glyco-beads4.The application of glyco-beads in the context of glyco-profiling and biomarker discoverywas closely described by Drake et al.5

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Table of Contents

General Information........................................................................................................................... 2

Intended Use ..................................................................................................................................... 4

Product description............................................................................................................................ 4

Kit Components................................................................................................................................. 5

Storage and lifetime information........................................................................................................ 5

Risk and safety information ............................................................................................................... 5

General remarks for sample handling and MALDI measurements ................................................... 6

Recommended sample preparation procedure ................................................................................. 7

Comments ......................................................................................................................................... 9

MALDI-TOF MS Target Preparation................................................................................................ 10

Cleaning of MALDI-MS targets.................................................................................................... 10Cleaning of AnchorChipTM targets ........................................................................................... 10Cleaning of polished steel targets ........................................................................................... 11

Matrix preparation on the MALDI-MS targets.............................................................................. 12Analysis of glycoproteins using 2,5-DHAP .............................................................................. 12Analysis of glycopeptides using 2,5-DHB................................................................................ 13Analysis of deglycosylated peptides using PAC targets.......................................................... 15

Example .......................................................................................................................................... 16

Isolation of Lactoferrin from a protein mixture ............................................................................. 16

References ...................................................................................................................................... 17

Trouble shooting.............................................................................................................................. 18

Ordering Information ....................................................................................................................... 19

Support............................................................................................................................................ 19

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Intended Use

The Glyco Kit MB-LAC WGA (Magnetic Bead-Lectin Affinity Chromatography with Wheat GermAgglutinin) is developed for specific capturing of N-glycosylated proteins derived from biologicalsamples based on lectin affinity prior to MALDI-TOF mass spectrometry analysis by means of e.g.Bruker´s microflex®, autoflex® or ultraflex® MALDI-TOF.

This kit is for research use only. It is not for use in diagnostic procedures.

Product description

The MB-LAC WGA kit is designed for enrichment and purification of N-glycosylated proteins priorto MALDI-TOF MS analysis. The MB-LAC WGA kit is based on super-paramagnetic microparticlesfunctionalized with Wheat Germ Agglutinin.WGA is a lectin which specifically binds to sialic acids and N-acetylglucosaminyl residues (seeFig. 3). Dimers and trimers of N-acetylglucosamine are preferentially bound. WGA can also bindoligosaccharides containing terminal N-acetylglucosamine or chitobiose, which are commonstructures of many serum and membrane glycoproteins6,7.

Glycoprotein

ManGlcNAc

FucGal

NeuAc

MB-LACWGA

GlycoproteinGlycoproteinGlycoprotein

ManGlcNAc

FucGal

NeuAc

ManGlcNAc

FucGal

NeuAc

MB-LACWGA

Fig. 3: Scheme of an N-glycan of the complex type. WGA-interacting residues are highlighted in orange.

Due to the binding requirements WGA binds N-glycans of the hybrid type and complex type. Highmannose type glycans are not bound because the required binding sites are not present.According to this, WGA beads can be applied as a tool for the capturing of N-glycosylated proteinswith broad specificity.

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Kit Components

Materials provided in the kits Abbreviation Remarks

Magnetic Beads: MB-LAC WGA Containing 0.02 % NaN3

MB-LAC WGA Binding Buffer BBMB-LAC WGA Wash Buffer 1 WB 1MB-LAC WGA Wash Buffer 2 WB 2MB-LAC WGA Elution Buffer EBDeionized water

Storage and lifetime information

Kit components should be stored and handled carefully to avoid contaminations.The kit is shipped at ambient temperature. We recommend storing the kit at 2-8°C after arrival. Donot freeze!If stored unopened under above conditions, the product is stable until expiration date mentioned onthe label.

Please note:After the first opening, period of storage should not exceed three months. For yourconvenience, we provide the possibility to mark the respective expiry date on the vial’s label.After usage, keep the vials closed. A drying up of the beads will damage the productirreversible!

Risk and safety information

The product does not have to be labeled due to the calculation procedure of the "GeneralClassification guideline for preparations of the EU" in the latest valid version. When used andhandled according to specifications, the product does not have any harmful effects according toour experience. All materials may present unknown hazards and should be used with caution.Observe the general safety regulations when handling chemicals.

Beside the kit components, we recommend further chemicals within these Instructions for Use.Please read and observe the respective Material Safety Data Sheet to be provided by yoursupplier. Observe the general safety regulations when handling chemicals.

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General remarks for sample handling and MALDI measurements

MALDI-TOF mass spectrometry is a very sensitive analysis method requiring special operationprocedures. Therefore, we recommend considering the following aspects before starting ananalysis:

The quality of the starting material will have an enormous impact on the outcome of theexperiments. Especially, collection and storage conditions have to be controlled carefully.When working with clinical samples, like serum or urine, please refer to the recentpublications of Baumann et al.8 and Fiedler et al.9 for standardized sample handlingprocedures.

To avoid contaminations all solvents used should be of HPLC grade or better

The plastic materials used for sample purification and matrix preparation should beexplicitly recommended for mass spectrometric measurements. Many common plasticsmay cause polymer contamination of the sample. This will interfere with the subsequentmatrix preparation/crystallization and produce polymer signals during spectra acquisition.

Sample purification and MALDI target preparation should be performed under cleanconditions. Dust and air pollution will decrease the spectra quality.

For optimal sample/matrix crystallization on the MALDI target the following environmentalconditions are recommended:

Operating temperature: 5 - 40°C (41 - 104°F)Operating humidity: 25 – 65 % at 22°CAtmospheric pressure: 75 - 105 kPa

For high resolution measurements and low signal to noise values a cleaning of the ionsource at regular intervals is absolutely mandatory. This procedure can be performed withthe Bruker Daltonics’ source shower target (see ordering information). For a completecleaning of the ion source please ask Bruker Daltonics’ service team([email protected]).

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Recommended sample preparation procedure

Binding of samples

1. Re-suspend the Magnetic Beads by shakingfor at least 20 times from top to the bottomand reversed or careful vortexing (do notsonificate!) to get a homogenous suspension.Repeat shaking between pipetting steps ifnecessary.

Re-suspend the beads

2. Transfer 20 µl of resuspended MagneticBeads to a standard thin wall PCR-tube andadd 100 µl WB 1.

20 µl beads

100 µl WB1

3. Place the tube in a Magnetic Separator andmove the tubes back and forth betweenadjacent wells 20 times. Wait for 20 secondsto separate the beads from the supernatant.

Move back and forth

Collect beads

4. Remove the supernatant carefully using apipette. Avoid contact of pipette tips with themagnetic beads and take care not to removethe beads.

Remove supernatant

5. Resuspend the beads in 100 µl WB 1 100 µl WB1

6. Repeat steps 3 and 4 to wash the beads andremove the supernatant Repeat steps 3 and 4

7. Re-suspend the beads in 10 µl BB and add upto 10 µl of the sample. If you have less samplevolume adjust to 10 µl by adding deionizedwater.For the analysis of serum, use 10 - 20 µlserum. Firstly, pipette an equal amount of BB(10 - 20 µl) to 20 µl magnetic beads andsubsequently add the serum.

10 µl BB

10 µl sample

8. Incubate the magnetic beads for 1 h at roomtemperature while swirling the tube from timeto time to mix the beads.

1 h incubation

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Washing of beads

9. Place the tube on the Magnetic Separator andwait for 20 seconds to separate the beadsfrom the supernatant. (Note the movement ofthe magnetic beads in the tube.)

Collect beads

10. Remove the supernatant carefully. Avoidcontact of pipette tips with the magnetic beadsand take care not to remove the beads.

Remove supernatant

11. Add 100 µl WB 1 100 µl WB1

12. Move the tubes back and forth betweenadjacent wells at least 20 times to wash thebeads.

Move back and forth

13. Wait 20 seconds for collecting magnetic beadsat the wall of the tubes and remove thesupernatant carefully.

Collect beads

14. Transfer the tube from the Magnetic Separatorto an alternative device. Add 100 µl WB2 andplace the tube back on the MagneticSeparator. Move the tubes back and forthbetween adjacent wells 20 times.

100 µl WB2

Move back and forth

15. Wait 20 seconds for collecting magnetic beadsat the wall of the tubes and remove thesupernatant carefully.

Collect beads

Remove supernatant

16. Repeat steps 14 and 15 once. Repeat steps 14 and 15

Elution of isolated proteins

17. For the elution of captured proteins add 10 µlEB and mix thoroughly. 10 µl EB

18. Incubate for 25 minutes while mixing from timeto time. 25 min incubation

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19. Place the tube on the Magnetic Separator andwait for 30 seconds for collecting the beads atthe wall of the tube.

Collect beads

20. Finally, transfer the elution buffer containingpurified glycoproteins into a fresh tube. Transfer eluate

Comments

Fast and successful collection of the magnetic beads is dependent on a high quality magneticseparation device. We strongly recommend the use of a Bruker Magnetic Separator (8-well,# 65554 or 96-well, # 207151).

The beads can also be applied for non-MS approaches. Depending on the subsequent techniquethe acidic elution solution may interfere with the following protocol steps. To remove the elutionsolution we recommend drying the eluate in a vacuum centrifuge. Afterwards, the dried sample canbe re-dissolved in an appropriate buffer or solution and processed according to the subsequentprotocol. This procedure is also recommended when different glyco-capturing particles are used insuccession.

Different glyco-beads can also be combined in one experiment to capture glyco-proteins withdifferent binding motifs at once. To do so, the buffer system of either a ConA or LCA kit has to beused since these buffer systems are compatible with the WGA and/or AIA beads, whereas thebuffers in the WGA and AIA kits are not suitable for the ConA or LCA functionality.

In principle, the amount of beads can be scaled up and down according to the experimentalrequirements. When doing this, it is important to keep the ratio of sample volume and WGA-BBvolume constant.

The provided buffer system has not been tested for the capturing of glycopeptides. Respectivebuffers will have to be optimized by the customer himself.

Please note:After elution using the provided EB, glyco-proteins are denatured. This will have an impact onfolding and functional activity.

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MALDI-TOF MS Target Preparation

MALDI-TOF MS target preparation is one of the most critical steps for high quality and reproducibleresults. Depending on the application, different target types and MALDI matrix preparations arefavored. For all applications it is mandatory to use a thoroughly cleaned target plate. Independence on the type of target Bruker Daltonic recommends different cleaning procedures.

Cleaning of MALDI-MS targets

Cleaning of AnchorChipTM targetsThis is an advanced cleaning protocol differing from the standard cleaning procedurerecommended in the product description.

1. Rinse the target intensively underflowing hot tap water Hot tap water

2. Wipe the target intensively with acetoneusing a kimwipe.

: Acetone is highly flammable andirritant (R: 11-36-66-67; S: (2)-9-16-26)

Acetone

3. Rinse the target with distilled water(Milli-Q). Distilled water

4. Rinse the target with methanol and let itdry.

: Methanol is highly flammable andtoxic (R: 11-23/24/25-39/23/24/25; S:(1/2)-7-16-36/37-45)

Methanol

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Cleaning of polished steel targetsThis is an advanced cleaning protocol differing from the standard cleaning procedurerecommended in the respective target’s product description.

1. Rinse the target intensively underflowing hot tap water. Hot tap water

2. Wipe the target intensively with acetoneusing a kimwipe.

: Acetone is highly flammable andirritant (R: 11-36-66-67; S: (2)-9-16-26)

Acetone


4. Wipe the target intensively with 80%TFA using a kimwipe.

: 80% TFA is corrosive (R: 20-35-52/53; S: (1/2)-9-26-27-28-45-61)

80 % TFA


6. Rinse the target with distilled water(Milli-Q). Distilled water

7. Rinse the target with methanol and let itdry.

: Methanol is highly flammable andtoxic (R: 11-23/24/25-39/23/24/25; S:(1/2)-7-16-36/37-45)

Methanol

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Matrix preparation on the MALDI-MS targets

Analysis of glycoproteins using 2,5-DHAP

For preparation of glycoproteins within the mass range of 6 – 100 kDa on AnchorChipTM 600targets (#209513) we recommend the use of 2,5-dihydroxyacetophenone (2,5-DHAP, #231829 or#231830) as matrix solution.

2,5-DHAP preparation on AnchorChip™600 targets

1. Dissolve 7.6 mg DHAP in 375 µlethanol.

: DHAP is irritant (R: 36/37/38; S:26-28-36/37-60), ethanol is highlyflammable (R: 11; S: (2)-7-16)

7.6 mg DHAP

375 µl ethanol

2. Add 125 µl (18 mg/ml in Milli-Q water)of a di-ammonium hydrogencitratesolution (= 10 µmol).

: Diammonium hydrogencitrate isirritant (R: 36/37; S: 26)

125 µl Diammonium

Hydrogen Citrate

3. Vortex for 1 min at RT. Vortex

4. Sonicate for 15 min. Sonificate

5. Vortex for 1 min at RT. The matrixsolution is stable for at least one weekwhen kept in the dark

Vortex

6. Mix 2 µl of the sample with 2 µl 2 %TFA and subsequently add 2 µl matrixsolution.

: 2% TFA is irritant (R: 36/38; S: 26-28-37-60)

2 µl Sample

2 µl 2% TFA

2 µl DHAP Matrix Solution

7. The mixture of sample, TFA and DHAPhas to be vigorously mixed by pipettingup and down. It is important that thesolution becomes turbid before spotting

Vigorously mixing

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8. 1 µl of the turbid suspension has to bespotted on an AnchorChipTM600 targetwithout generating air bubbles.

Spot 1 µl

9. After drying of the spots the target canbe transferred to the MALDI-TOF MSfor measurements.

Dry the spot

Analysis of glycopeptides using 2,5-DHB

For preparation of glycopeptides (e.g. after proteolytic digest of the glycoprotein) within the massrange of 1 – 10 kDa we recommend the use of AnchorChip™ 600 targets (#209513) or polishedsteel targets (#209520) and 2,5-Dihydroxybenzoic acid (DHB, #201346 or #203074) as matrix.

2,5-DHB preparation on AnchorChip™600 targets

1. Dissolve 5 mg DHB in 300 µlacetonitrile

: DHB is harmful (R: 22-36/37/38;S: 26-28-36/37-60), acetonitrile ishighly flammable and harmful (R: 11-20/21/22-36; S: (1/2)-16-36/37)

5 mg DHB

300 µl acetonitrile

2. Add 700 µl TFA (0.1 %) 700 µl 0.1 %TFA

3. Vortex for 1 min at RT Vortex

4. Sonificate for 15 min Sonificate

5. 1 µl of the freshly prepared matrix has tobe spotted onto an AnchorChip™ 600target

Spot 1 µl matrix

6. After drying spot 1 µl of sample onto thematrix Spot 1 µl sample

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7. After drying of the spots the target canbe transferred to the MALDI-TOF MS formeasurements.

Dry the spot

2,5-DHB preparation on polished steel targets

1. Dissolve 10 mg DHB in 300 µlacetonitrile

: DHB is harmful (R: 22-36/37/38; S:26-28-36/37-60), acetonitrile is highlyflammable and harmful (R: 11-20/21/22-36; S: (1/2)-16-36/37)

10 mg DHB

300 µl acetonitrile

2. Add 700 µl TFA (0.1 %) 700 µl 0.1 %TFA

3. Vortex for 1 min at RT Vortex

4. Sonificate for 15 min Sonificate

5. 1 µl of the freshly prepared matrix has tobe spotted onto a polished steel target Spot 1 µl matrix

6. After drying spot 1 µl of sample onto thematrix Spot 1 µl sample

7. After drying of the spots the target can betransferred to the MALDI-TOF MS formeasurements.

Dry the spot

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Analysis of deglycosylated peptides using PAC targets

For the analysis of deglycosylated peptides (e.g. after PNGase F treatment) the use of PAC (Pre-spotted AnchorChip) targets prespotted with α-Cyano-4-hydroxycinnamic acid (HCCA) isrecommended (#231968 or #227463).

Sample preparation onto pre-spotted disposable AnchorChip targets

1. Deposit 0.3 – 3 µl sample solution ontothe matrix spot. The acetonitrileconcentration of the sample must notexceed 40 %

Spot 0.3 – 3 µl sample

2. Incubate the sample for 3 min onto thespot, but do not let the droplets dry 3 min incubation

3. Remove the residual solvent Remove droplet

4. Add 7 – 10 µl of washing buffer (10mMAmmonium phosphate, monobasic, in0.1 % TFA)

Wash with 7 – 10 µl

5. Incubate for a few second andsubsequently remove the wash buffer

Remove wash buffer

after a few seconds

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Example

Isolation of Lactoferrin from a protein mixture

Sample: Mixture of Bruker Protein Standard II (36 pmol),RNase B (30 pmol) and Lactoferrin (30 pmol)

Beads: MB-LAC WGA (20 µl)Target: AnchorChip™ 600/384Matrix: 2,5-DHAPMALDI mode: Linear

66475

0

200

400

600

[RNaseB+H]+14847

0

200

400

600

800

1000

Inte

ns.[

a.u.

]

[WGA+H]+17228

[LF+H]+84212

0

250

500

750

1000

1250

20000 30000 40000 50000 60000 70000 80000 90000m/z

[BSA+H]+66404

[Prot.A+H]+44521

[Trypsinogen+H]+23944

[BSA+2H]2+

[RNaseB+H]+14887


[LF + H]+82796

[Prot.A+H]+44561

Original mixture

Supernatant

Eluate

66475

0

200

400

600

[RNaseB+H]+14847

0

200

400

600

800

1000

Inte

ns.[

a.u.

]

[WGA+H]+17228

[LF+H]+84212

0

250

500

750

1000

1250

20000 30000 40000 50000 60000 70000 80000 90000m/z

[BSA+H]+66404

[Prot.A+H]+44521


[BSA+2H]2+

[RNaseB+H]+14887


[LF + H]+82796

[Prot.A+H]+44561

Original mixture

Supernatant

Eluate

Lactoferrin ([M+H]+ 84 kDa; [M+2H]2+ 42 kDa) was successfully captured from a mixture of ProteinStandard II (BSA: [M+H]+ 66 kDa; [M+2H]2+ 33 kDa; Protein A: [M+H]+ 44 kDa; Trypsinogen:[M+H]+ 24 kDa), RNase B ([M+H]+ 15 kDa) and Lactoferrin. Only lactoferrin representing thecorresponding binding motif for WGA was enriched but not the high mannose type glycoproteinRNase B. The peak at 17 kDa in the eluate represents WGA which is also partially released duringthe elution process..

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References

1 Sparbier K, Koch S, Kessler I, Wenzel T, and Kostrzewa M.(2005). Selective Isolation ofGlycoproteins and Glycopeptides for MALDITOFMS Detection Supported by Magnetic Particles. JBiomol Tech, 16: 407 – 413.

2 Sparbier K, Wenzel T, Kostrzewa M. (2006). Exploring the binding profiles of ConA, boronic acidand WGA by MALDI-TOF/TOF MS and magnetic particles. J ChromB, 840: 29–36.

3 Sparbier K, Asperger A, Resemann A, Kessler I, Koch S, Wenzel T, Stein G, Vorwerg L, SuckauD, Kostrzewa M. (2007). Analysis of Glycoproteins in Human Serum by Means of Glyco-specificMagnetic Bead Separation and LC-MALDI-TOF/TOF Analysis with Automated GlycopeptideDetection. J Biomol Tech, 18: 252–258.

4 Sparbier K; Kessler I, Koch S, Mix G, Kostrzewa M. (2007). CLINPROT Glyco-beads: Verificationof Long-Term Stability and Batch-to-Batch Reproducibility. Technote Bruker Daltonic (tn-20).

5 Drake RR, Schwegler EE, Malik G, Diaz J, Block T, Mehta A, and Semmes OJ. (2006). LectinCapture Strategies Combined with Mass Spectrometry for the Discovery of Serum GlycoproteinBiomarkers. Mol&CellProt, 5.10:1957 – 1967.

6 Rueben, L., et al., (1977). Activities of lectins and their immobilized derivatives in detergentsolutions. Implications on the use of lectin affinity chromatography for the purification of membraneglycoproteins. Biochemistry, 16, 1787-1794

7 Allen, A. K., et al., (1973).The purification, composition and specificity of wheat-germ agglutinin.Biochem. J., 131, 155-162.

8 Baumann S, Ceglarek U, Fiedler GM, Lembcke J, Leichtle A, Thiery J. (2005): Standardizedapproach to proteome profiling of human serum based on magnetic bead separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Clin Chem., 51, 973-80.

9 Fiedler GM, Baumann S, Leichtle A, Oltmann A, Kase J, Thiery J, Ceglarek U. (2007):Standardized peptidome profiling of human urine by magnetic bead separation and matrix-assistedlaser desorption/ionization time-of-flight mass spectrometry. Clin Chem. 53, 421-8.

Trouble shooting

Problem Reason What to do

Sample does not crys-tallize, crystallization looksstrange

Plastics (tips, tubes, flasks)are not MS-compatible

Solvents are not of HPLCgrade or better

Environmental conditionsare not compatible with MSpreparations

Change plastics (referr tothe ording information forMS compatible plastics

Exchange the solvents anduse higher quality solvents

Prepare under a cleanbench, check humidity,maybe humidify the room,switch off the air condition

Polymer contaminations Plastics (tips, tubes, flasks)are not MS-compatible

Solvents are not of HPLCgrade or better

Change plastics (refer tothe ordering information forMS compatible plastics)

Exchange the solvents anduse higher quality solvents

Low signal/noise values Environmental conditionsare not compatible with MSpreparations

Air pollution and sub-sequent contamination withsodium or potassium

Quality of the startingmaterial

Prepare under a cleanbench, check humidity,maybe humidify the room,switch off the air condition

See above

No detection of glyco-proteins/-peptides

Glyco-proteins/-peptides donot represent the requiredbinding motifs

Try other glyco-beadsfunctionalized with anotherlectins or boronic acidsbeads

Ordering Information

Product Size (purifications orapplications per kit) Part No.

Glyco Kit 20 MB-LAC WGA 20 244669

Glyco Kit 20 MB-CovAC boronic 20 241104Glyco Kit 20 MB-LAC Con A 20 252665Glyco Kit 20 MB-LAC LCA 20 246534Glyco Kit 20 MB-LAC AIA 20 250359

Magnetseparator 016 8-well 65554Magnetseparator 104 II 96-well 207151

2,5-Dihydroxyacetophenone, 1g 2318292,5-Dihydroxybenzoic acid, 1 g 2013462,5-Dihydroxybenzoic acid, 5 g 203074α-Cyano-4-hydroxycinnamic acid, 1g 201344α-Cyano-4-hydroxycinnamic acid, 5g 203072

MTP AnchorChip™ 600/384 T F 209513PAC96 Set for Proteomics 231968PAC384 Set for Proteomics 227463MTP PAC Frame 221598MTP 384 target plate polished steel T F 209520MTP Ion Source Shower Target 226054

8-Well Strips 60550CapStrips 6055196-Well TubePlates 61565

Bruker Daltonics’ Recommendations

Pipette tips Eppendorf standard tipsVials Eppendorf safe-lockBottles Nalgene PP

SupportEmail: [email protected]: +49 (421) 2205-0Web: www.care-bdal.de

For research use only. Not for use in diagnostic procedures.

Revision 1, January 2012Copyright 2012 Bruker Daltonik GmbHDescriptions and specifications supersede all previous information and are subject to change without notice.

Instructions for Use - Glyco Kit MB-LAC WGA

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