Instructions For Use

FA361.5Fixed-Angle Rotor

For Use in Beckman CoulterMicrofuge 20 Series Centrifuges

June 2013

Beckman Coulter, Inc.250 S. Kraemer Blvd.Brea, CA 92821 U.S.A.

PN B30182AA

FA361.5 Fixed-Angle RotorB30182AA (June 2013)

Copyright 2013 Beckman Coulter, Inc.

All rights reserved. No part of this document may be reproduced or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without prior written permission from Beckman Coulter, Inc.

Beckman Coulter and the stylized logo are trademarks of Beckman Coulter, Inc. and are registered in the USPTO.

All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.

Find us on the World Wide Web at: www.beckmancoulter.com

Made in GermanyPrinted in U.S.A.

Safety Notice

Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to use this equipment. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative.

Alerts for Warning, Caution, and Note

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury.

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices.

NOTE NOTE is used to call attention to notable information that should be followed during installation, use,

or servicing of this equipment.

Safety Information for the FA361.5 Rotor

Handle body fluids with care because they can transmit disease. No known test offers complete assurance that such fluids are free of micro-organisms. Some of the most virulent—Hepatitis (B and C) viruses, HIV (I–V), atypical mycobacteria, and certain systemic fungi—further emphasize the need for aerosol protection. Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this rotor without taking appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection.

The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors, or a hazardous chemical reaction. Do not centrifuge such materials in nor handle or store them near the centrifuge.

These rotors were developed, manufactured, and tested for safety and reliability as part of a Beckman Coulter centrifuge/rotor system. Their safety or reliability cannot be assured if used in a non-Beckman Coulter centrifuge, in a Beckman Coulter centrifuge not specified for use with the rotors, or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter’s approval.

PN B30182AA iii

Safety NoticeSafety Information for the FA361.5 Rotor

Although rotor components and accessories made by other manufacturers may fit in the FA361.5 rotor, their safety in these rotors cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the FA361.5 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer. Only the components and accessories listed in this publication should be used in these rotors.

Make sure that filled containers are loaded symmetrically into the rotor and that opposing tubes are filled to the same level with liquid of the same density.

If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories.

Never exceed the maximum rated speed of the rotor and labware in use. Refer to the section on Run Speeds.

Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins in scratches and may open fissures in the rotor with continued use.

PN B30182AAiv

Contents

Safety Notice, iii

Alerts for Warning, Caution, and Note, iii

Safety Information for the FA361.5 Rotor, iii

FA361.5Fixed-Angle Rotor, 1

Specifications for the FA361.5 Rotor, 1

Description, 2

Preparation and Use, 2Prerun Safety Checks, 3

Rotor Preparation, 3

Operation, 4

Removal and Sample Recovery, 4Tubes, 5

Temperature Limits, 5

Run Speeds, 6

Care and Maintenance, 6Maintenance, 6Cleaning, 7Decontamination, 8Sterilization and Disinfection, 9Storage, 9

Returning a Rotor, 10

Supply List, 10Replacement Rotor Parts, 10Supplies, 11

Beckman Coulter, Inc.Benchtop Rotor Warranty

Related Documents

v

vi

Tables

Tables

1 Available Tubes for the FA361.5 Rotor, 5

FA361.5Fixed-Angle Rotor

Specifications for the FA361.5 Rotor

1. Axis of Rotation

Maximum speed 15,000 RPM

Density rating at maximum speed 1.2 g/mL

Critical speed rangea 800 to 1200 RPM

Relative Centrifugal Fieldb at maximum speed At rmax (80.0 mm) 20,124 g

Conditions requiring speed reductions see Run Speeds

Maximum imbalance of opposing loads 6 grams

Number of tube cavities 36

Available bottles and tubes see Table 1

Nominal tube dimensions 10 31 mm

Nominal tube capacity (largest tube) 2 mL

Nominal rotor capacity 72 mL

Approximate acceleration time to maximum speed (fully loaded) 36 sec

Approximate deceleration time from maximum speed (fully loaded) 20 sec

Weight of fully loaded rotor 1.24 kg (2.73 lb)

Rotor material aluminum

a. The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass. Passing through the critical

speed range is characterized by some vibration.

b. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (r2) to the standard acceleration of

gravity (g) according to the following formula: RCF = r2/g — where r is the radius in millimeters, is the angular velocity in radians per

second (2 RPM /60), and g is the standard acceleration of gravity (9807 mm/s2). After substitution: RCF = 1.12r (RPM/1000)2

PN B30182AA 1

FA361.5 Fixed-Angle RotorDescription

Description

The Beckman Coulter FA361.5 rotor has been manufactured in a facility that maintains certifications to both ISO 9001:2008 and ISO 13485.2003. It is for use with the specified Beckman Coulter centrifuges.

The FA361.5 is a fixed-angle rotor with two rows of cells holes. The inner row has a tube angle of 50 degrees, and the outer row has a tube angle of 30 degrees from the axis of rotation. This rotor can centrifuge up to thirty-six 1.5 to 2.0-mL reaction-vials, as well as 250 to 750-L vials. This rotor develops centrifugal forces that can efficiently pellet subcellular organelles, viruses, bacteria, mitochondria, chloroplasts, or algae.

The FA361.5 rotor is made of aluminum and is black-anodized for corrosion resistance. A screw-on lid (B31098), made of anodized aluminum, ships with the rotor.

The FA361.5 rotor, with lid B31098, was tested* to demonstrate containment of microbiological aerosols under normal operating conditions of the associated Beckman Coulter centrifuge, when used and maintained as instructed.

Refer to the Warranty at the back of this manual for warranty information

Preparation and Use

Specific information about the FA361.5 rotor is given here. Information about the centrifuges is contained in the Microfuge 20 Series centrifuge manual, which should be used together with this manual for complete centrifuge, rotor, and accessory operation.

NOTE Although rotor components and accessories made by other manufacturers may fit in the FA361.5

rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in this rotor may void the rotor warranty and should be prohibited by your laboratory safety officer. Only the components and accessories listed in this publication should be used in this rotor.

1. Screw-on Lid (B31098)

2. Rotor O-ring (368990)

3. Rotor Body

* Validation of microbiological containment was done at an independent third-party testing facility (CAMR, Porton Down,

UK, or USAMRIID, Ft. Detrick, MD, U.S.A.). Improper use or maintenance may affect seal integrity and thus

containment.

PN B30182AA2

FA361.5 Fixed-Angle RotorRotor Preparation

Prerun Safety Checks

Read the Safety Notice section at the front of this manual before using the rotor.

1 Make sure that the rotor, lid, and all tubes and accessories are clean and show no signs of

corrosion or cracking.

2 If fluid containment is required, use capped tubes.

All containers carrying physiological fluids should be capped, and not overfilled, to prevent leakage.

Verify that the bottles or tubes being used are listed in Table 1.

3 Check the chemical compatibilities of all materials used (refer to Chemical Resistances,

publication IN-175).

Rotor Preparation

For runs at other than room temperature, refrigerate or warm the rotor before the run for fast equilibration.

1 Be sure that the metal threads in the rotor are clean and lightly but evenly coated with Spinkote

lubricant (306812).

2 Load the filled containers symmetrically into the rotor (see Tubes, page 5 for tube information).

If fewer than 36 tubes are being run, they must be arranged symmetrically in the rotor.

Each row must have tubes that have been loaded symmetrically in the rotor.

Opposing tubes must be filled to the same level with liquid of the same density.

3 Put the lid in place and tighten it by hand.

PN B30182AA 3

FA361.5 Fixed-Angle RotorOperation

Operation

Precool the rotor in the centrifuge or in a refrigerator before use—especially before short runs—to ensure that the rotor reaches the set temperature. A suggested precooling cycle is a minimum of 30 minutes at 2000 RPM with the temperature set to 0°C.

NOTE Temperatures may vary slightly between centrifuges. If sample temperature is crucial, test

temperature settings on your instrument using water samples. For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration.

1 Ensure that the rotor tie-down screw (369554) is in good condition and the threads are free of

foreign matter.

CAUTION

Do not drop the rotor onto the drive shaft. The shaft can be bent or damaged if the rotor is forced sideways or dropped onto the shaft.

2 Center the rotor over the drive shaft and carefully lower it straight down.

3 Secure the rotor to the drive shaft with the tie-down screw.

Tighten the screw by turning it to the right (clockwise) with the T-handle rotor wrench (361371).

4 Refer to the instrument instruction manual for centrifuge operation.

See Run Speeds, page 6, for information about speed limitations.

Removal and Sample Recovery

CAUTION

If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories.

1 Using the T-handle rotor wrench, turn the tie-down screw to the left (counterclockwise) to

release the rotor.

2 Remove the rotor by lifting it straight up and off the drive shaft.

PN B30182AA4

FA361.5 Fixed-Angle RotorRemoval and Sample Recovery

3 Remove the rotor lid by unscrewing it counterclockwise and remove the tubes.

Tubes

The FA361.5 rotor holds up to thirty-six 1.5 to 2.0 -mL reaction-vials, listed in Table 1. The rotors also hold 250 to 750-L vials in adapters

Refer to Chemical Resistances (IN-175) for information on the chemical compatibilities of labware materials.

Temperature Limits

• Plastic containers have been centrifuge tested for use at temperatures between 2 and 25°C. For centrifugation at other temperatures, pretest containers under anticipated run conditions.

Table 1 Available Tubes for the FA361.5 Rotor

Description Dimensions Volume PartNumber

Adapter Max Speed(RPM)

polyethylene tube with attached cap

11 45 mm 1.8 mL 340196(pkg/500)

364701(pkg/12)

10,000

polypropylene tube with attached cap (natural)

11 40 mm 1.5 mL 357448(pkg/500)

364701(pkg/12)

15,000

polypropylene tube with attached cap

11 40 mm 1.5 mL 343169(pkg/500)

364701(pkg/12)

15,000

polyethylene tube, plain

7 40 mm 400 L 314326(pkg/1000)

361247(pkg/24)

11,300

polyethylene tube, Heparin-Lithium Fluoride coated

7 40 mm 400 L 652824(pkg/1000)

361247(pkg/24)

11,300

polyethylene tube, Heparin-Lithium coated

7 40 mm 400 L 652825(pkg/1000)

361247(pkg/24)

11,300

polyethylene tube, Heparin-Lithium Fluoride coated

5 45 mm 250 L 652821(pkg/1000)

361247(pkg/24)

11,300

polyethylene tube, Heparin-Lithium coated

5 45 mm 250 L 652822(pkg/1000)

361247(pkg/24)

11,300

polyethylene tube, plain

5 45 mm 250 L 652823(pkg/1000)

361247(pkg/24)

11,300

PN B30182AA 5

FA361.5 Fixed-Angle RotorRun Speeds

• If plastic containers are frozen before use, make sure that they are thawed to at least 2°C before centrifugation.

Run Speeds

The centrifugal force at a given radius in a rotor is a function of speed. Comparisons of forces between different rotors are made by comparing the rotors’ relative centrifugal fields (RCF). When rotational speed is adjusted so that identical samples are subjected to the same RCF in two different rotors, the samples are subjected to the same force. The RCF at each speed is automatically calculated by the centrifuge software; if the RCF is entered, the centrifuge calculates the equivalent revolutions per minute (RPM).

The maximum run speed listed in the rotor specifications is for operation when all conditions are within the standard specifications. Speeds must be reduced under the following circumstances:

1. If nonprecipitating solutions more dense than 1.2 g/mL are centrifuged, the maximum allowable run speed must be reduced according to the following equation:

where is the density of the tube contents. This speed reduction will protect the rotor from excessive stresses due to the added tube load. Note, however, that the use of this formula may still produce maximum speed figures that are higher than the limitations imposed by the use of certain tubes or adapters. In such cases, use the lower of the two figures.

2. Further speed limits must be imposed when self-forming-gradient salts are centrifuged, as the equation does not predict concentration limits/speeds that are required to avoid precipitation of salt crystals.

Care and Maintenance

Maintenance

Do not use sharp tools on the rotor, as they can scratch the rotor surface. Corrosion begins in scratches and may open fissures in the rotor with continued use.

reduced maximum speed = (15,000 RPM) 1.2 g/mL

---------------------

1. Screw-on Lid

2. Rotor Body

3. Check for Corrosion

4. Rotor O-ring

5. Rotor lid O-ring

PN B30182AA6

FA361.5 Fixed-Angle RotorCare and Maintenance

1 Periodically (at least monthly) inspect the rotor, especially inside cavities, for rough spots,

cracks, pitting, white powder deposits (frequently aluminum oxide), or heavy discoloration.

If any of these signs are evident, do not run the rotor.

2 Before using the tie-down screw (369554), check it for damage such as stripped threads.

Replace it if it is damaged.

3 Check the rotor O-ring (368890) and lid O-ring (368896) for nicks, abrasions, or other damage

and replace damaged components before using the rotor.

Do not use a rotor with damaged components.

Apply a thin, even coat of silicone vacuum grease (335148) to the O-ring, then replace it in the groove in the outer rim of the rotor.

4 Regularly apply a thin, even coat of Spinkote lubricant (306812) to the rotor drive hole to

prevent rotor sticking.

Refer to Chemical Resistances for the chemical compatibilities of rotor and accessory materials. Contact your Beckman Coulter Representative for information about the Field Rotor Inspection Program and the rotor repair center.

Cleaning

Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred. Do not allow corrosive materials to dry on the rotor.

Under normal use, wash the rotor frequently (at least weekly) to prevent buildup of residues. If the rotor is left in the centrifuge for long periods of time, remove it at least once a month for cleaning and lubrication.

1 Remove the O-ring and wash the rotor, lid, and O-ring in a mild detergent, such as Solution 555

(339555), that won’t damage the rotor.

Dilute the detergent with water (10 parts water to 1 part detergent).

The Rotor Cleaning Kit (339558) contains two plastic-coated brushes and two quarts of Solution 555 for use with rotors and accessories.

PN B30182AA 7

FA361.5 Fixed-Angle RotorCare and Maintenance

NOTE Do not wash the rotor components or accessories in a dishwasher. Do not soak components in

detergent solution for long periods of time, such as overnight.

2 Thoroughly rinse the cleaned rotor and components with distilled water.

3 Air-dry the rotor and lid upside down.

Do not use acetone to dry the rotor.

4 Lightly but evenly lubricate the rotor drive-hole threads with Spinkote (306812).

5 Apply a thin, even coat of silicone vacuum grease (335148) to the O-ring, then replace it in the

groove in the outer rim of the rotor.

6 Clean metal threads every 6 months, or as necessary.

• Use a brush and concentrated Solution 555.

• Rinse and dry thoroughly, then lubricate lightly but evenly with Spinkote to coat all threads.

Decontamination

If the rotor (and/or accessories) becomes contaminated with radioactive material, decontaminate it using a solution that will not damage the anodized surfaces. Beckman Coulter has tested a number

1. O-ring

2. Rotor Body

PN B30182AA8

FA361.5 Fixed-Angle RotorCare and Maintenance

of solutions and found two that do not harm anodized aluminum: RadCon Surface Spray or IsoClean

Solution (for soaking),* and Radiacwash.†

NOTE IsoClean can cause fading of colored anodized surfaces. Use it only when necessary and remove it

promptly from surfaces.

While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of decontamination is expressed or implied. Consult your laboratory safety officer regarding the proper decontamination methods to use.

If the rotor or accessories are contaminated with toxic or pathogenic solutions, follow appropriate decontamination procedures. Check Chemical Resistances (IN-175) to be sure the decontamination method will not damage any part of the rotor.

Sterilization and Disinfection

• The rotor and metal rotor components can be autoclaved at 121°C for up to one hour. Remove the lid from the rotor and place the rotor, lid, and O-ring in the autoclave upside down.

• The rotor lid can be autoclaved at 115°C to 118°C for up to 40 minutes. Remove the O-ring from the lid and place the lid and O-ring in the autoclave.

• Ethanol (70%) or hydrogen peroxide (6%) may be used on all rotor components, including those made of plastic. Bleach (sodium hypochlorite) may be used on the rotor body, but may cause discoloration of anodized surfaces. Do not use bleach on plastic rotor components. Use the minimum immersion time for each solution, per laboratory standards.

CAUTION

Ethanol is a flammability hazard. Do not use it in or near operating centrifuges.

While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

Refer to publication IN-192, included in each box of tubes or bottles, for tube sterilization and disinfection procedures.

Storage

When the rotor is not in use, store it in a dry environment (not in the centrifuge) with the rotor lid removed to allow air circulation so moisture will not collect in the tube cavities.

* In U.S., contact Nuclear Associates (New York); in Eastern Europe and Commonwealth States, contact Victoreen GmbH

(Munich); in South Pacific, contact Gammasonics Pty. Ltd. (Australia); in Japan, contact Toyo Medic Co. Ltd. (Tokyo).

† In U.S., contact Biodex Medical Systems (Shirley, New York); internationally, contact the U.S. office to find the dealer

closest to you.

PN B30182AA 9

FA361.5 Fixed-Angle RotorReturning a Rotor

Returning a Rotor

Before returning a rotor or accessory for any reason, prior permission must be obtained from Beckman Coulter, Inc. The authorization form may be obtained from your local Beckman Coulter sales office. The form, entitled Returned Material Authorization (RMA) for United States returns or Returned Goods Authorization (RGA) for international returns, should contain the following information:

• rotor type and serial number,

• history of use (approximate frequency of use),

• reason for the return,

• original purchase order number, billing number, and shipping number, if possible,

• name and email address of the person to be notified upon receipt of the rotor or accessory at the factory,

• name and email address of the person to be notified about repair costs, etc.

To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from pathogens and/or radioactivity. Sterilization and decontamination must be done before returning the parts. Smaller items (such as tubes, bottles, etc.) should be enclosed in a sealed plastic bag.

All parts must be accompanied by a note, plainly visible on the outside of the box or bag, stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach this notification will result in return or disposal of the items without review of the reported problem.

Use the address label printed on the RGA/RMA form when mailing the rotor and/or accessories.

Customers located outside the United States should contact their local Beckman Coulter office.

Supply List

Call Beckman Coulter Customer Service at 1-800-742-2345 (U.S.A. or Canada) or contact your local Beckman Coulter Representative for detailed information on ordering parts and supplies. For your convenience, a partial list is given below.

Replacement Rotor Parts

Description Part Number

FA361.5 rotor assembly B30157

Lid assembly, BioSafe B31098

Rotor O-ring 368890

Lid O-ring 368896

Tie-down screw 369554

T-handle wrench 361371

PN B30182AA10

FA361.5 Fixed-Angle RotorSupply List

Supplies

NOTE For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com.

Description Part Number

Tubes and accessories see Table 1

Adapter for 750 L vials (pkg/12) 364690

Spinkote lubricant (2 oz) 306812

Silicone vacuum grease (1 oz) 335148

Rotor Cleaning Kit 339558

Solution 555 (1 qt) 339555

PN B30182AA 11

FA361.5 Fixed-Angle RotorSupply List

PN B30182AA12

Beckman Coulter, Inc.Benchtop Rotor Warranty

Subject to the conditions specified below and the warranty clause of the Beckman Coulter, Inc., terms and conditions in effect at the time of sale, Beckman Coulter agrees to correct either by repair or, at its election, by replacement, any defects of material or workmanship which develop within seven (7) years after delivery of a benchtop centrifuge rotor to the original buyer by Beckman Coulter or by an authorized representative, provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use. Should a Beckman Coulter centrifuge be damaged due to a failure of a rotor covered by this warranty, Beckman Coulter will supply free of charge all centrifuge parts required for repair.

ConditionsExcept as otherwise specifically provided herein, this warranty covers the rotor only and Beckman Coulter shall not be liable for damage to accessories or ancillary supplies including but not limited to (i) tubes, (ii) tube caps, (iii) tube adapters, or (iv) tube contents.

This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance contrary to the instructions in the Beckman Coulter rotor or centrifuge manual.

This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge unmatched to the rotor characteristics or operated in a Beckman Coulter centrifuge that has been improperly disassembled, repaired, or modified.

Thermoplastic rotors or components used in some benchtop centrifuges are warranted for one (1) year from date of purchase.

DisclaimerIT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND THAT NEITHER BECKMAN COULTER, INC. NOR ITS SUPPLIERS SHALL HAVE ANY LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR, MAINTENANCE, OR REPLACEMENT OF THE PRODUCT.

PN B30182AA Warranty-1

Beckman Coulter, Inc. Benchtop Rotor Warranty

PN B30182AAWarranty-2

© 2013 Beckman Coulter, Inc.

All Rights Reserved

www.beckmancoulter.com

Related Documents

Microfuge 20 Series Centrifuges (B29070)

• Safety

• Introduction

• Description

• Operation

• Troubleshooting

• Care and Maintenance

• Installation

Available in hard copy or electronic pdf by request.

Chemical Resistances for Beckman Coulter Centrifugation Products (IN-175)

Use and Care of Centrifuge Tubes and Bottles (IN-192)

Available in hard copy or electronic pdf by request.

Available at www.beckmancoulter.com