8/19/2019 Instructions for MICRO99

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Fera

Sand Hutton

York

YO41 1LZ

Tel: +44 (0)1904 462100Fax: +44 (0)1904 500440

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LEAP® Scheme Potable Water MicrobiologyDistribution MICRO99 ests !"#99$ !"%99$ !"&'9$ an( !")*'

#+ March %"#'

Please note that safety information relating to the handlingof LEAP® Scheme Micro samples can be downloaded fromour website www.fapas.com/pdfpub/LeapMicro_safetysheet.pdf

Simulate( Potable or ,ottle( Water

 !ou ha-e been sent.

ne or more of the following test materials! as appropriate to your order"

est Material A for !"#99 #otal $oliforms A%& Escherichia coli  

est Material , for !"%99 $olony $ounts after ' days at (()$ A%& $olony $ounts after ( days at '*)$

est Material C for !"&'9 Enterococci and $lostridium perfringens

est Material D for !")*' rganism +dentification

/orm o0 test materials.

est Materials A $, an( C.#he samples are presented as a small plastic ,ial containing aninoculum tablet together with (- ml .-ml sterile diluent in aplastic bottle.

est Material D" +s a nutrient agar slope which has been inoculated with anorganism that may routinely be reco,ered from water. Pleasenote that the organism may be 0ram 1,e or 0ram 2,e.

Storage o0 est Materials

3.  Store the SMALL plastic ,ial4s5 at 61()$. 

(.  Store the bottle4s5 at 7M #EMPE7A#87E.

'.  Store test material & at 29)$4()$5. 

Instructions 0or analysis an( re1ort o0 results3.  Start the analyses on Mon(ay %# March or ues(ay %% March %"#'2 

(.  +# +S ESSE%#+AL that you prepare test materials A! : and $ as specified in the  ;+%S#78$#+%S document attached beforeyou start the analyses.

'. /or !"#99. a0ter 1re1aration o0  test material A analyse it for #otal $oliforms A%&Escherichia coli and report the result for each in cfu/3ml.

9. /or !"%99. a0ter 1re1aration o0 test material , carry out a $olony $ount after ' days

at (()$ and a $olony $ount after ( days at '*)$ and report the result for each in cfu/ml. 

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-. /or !"&'9. a0ter 1re1aration o0 test material C analyse it for Enterococci and$lostridium perfringens and report the result for each in cfu/3ml. 

?. /or !")*'. Sample & re@uires no preparation. +dentify the organism growing on thesurface of the agar and report the identification to species le,el in full if this is what yourlaboratory would usually do! if not report to the genus le,el.

*.  +nstructions on how to enter your results and methods ,ia the secure web page can bedownloaded from our website at www.fapas.com/pdfpub/es_instructions.pdf . Please ensureyou submit your data no later than"

closing date - April (3?

 ou are reminded that the ability to report results in the specified units and within the gi,en timescale are part of the proficiency test.

Please note that collusion between participants is contrary to professional scientific conduct and! as

indicated in our Protocol 4see www.fapas.com/pdfpub/

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37O99 Te$t$ Y0199 Y0299 Y0469 *'? 

re#araton n$tru&ton$ 8 a-e 1 o! 1 

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