Insect Body Plan and Hox genes - home.sandiego.eduhome.sandiego.edu › ... ›...
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Insect Body Plan and Hox genes Lyn Harder and Claire Kuelbs
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What are Hox genes?
● Discovered in the 1980s in Drosophila ● Homeobox-containing genes
● Play a big role in segment identity
➔ Encode transcription factors that activate or repress different genes
● If changed in any way, can cause homeotic
transformation
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What are homeotic transformations? Replacement of one body part with another
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What are features of Hox genes? ● Clustered ● Colinear
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Do other organisms have Hox genes?
What would happen if Hox genes never mutated?
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How are Hox genes studied? ● Often studied in insects
➔ Why are insects ideal for studying Hox genes?
● RNA interference
➔ Uses double stranded RNA to target a gene product causing degradation
➔ Creates a silencing of the gene
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Insect body plan ● What are the most defining features of the
insect body plan?
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HOX genes and the insect body plan ● Ultrabithorax (Ubx)
➔ Expressed in the abdominal segments
➔ Suppresses thoracic leg development
➔ What happens when ubx is ectopically expressed in the thoracic segments?
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How did Ronshaugen et al. (2002) study the function of Ubx? ● Comparisons between Drosophila and the
crustacean Artemia franciscana ➔ Why did they choose A. franciscana?
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Is there a difference in function between the two species? ● Artemia Ubx was only able to suppress 15%
of thoracic limb development when ectopically expressed in Drosophila
➔ Drosophila suppressed all limbs
● Artemia Ubx transformed thoracic denticles towards an abdominal identity
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Why is there a difference in the ability to repress thoracic limbs?
● Two hypotheses
➔ The Drosophila Ubx C-terminal has a limb repressing domain while the Artemia Ubx C-terminal is not sufficient for limb repression
➔ The Artemia C-terminal sequences regulate a limb
repression domain elsewhere in both Ubx proteins
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Figure 3: Repression of thoracic limbs by hybrid proteins
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Artemia Ubx inhibits a limb repression domain ● When the Artemia C-terminus was deleted,
80% of thoracic limbs were repressed
➔ Big shift from 15%
● When the Drosophila C-terminus was deleted, 80% of thoracic limbs were repressed
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Discussion Question What environmental or ecological constraints might have caused the need for limb repression within hexapods and therefore cause the divergence of the taxa?
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Discussion Question What are the benefits of evolving, as insects have from other crustaceans, to having your limbs restricted to developing on one segment of the body, while repressing growth on other segments?
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What contributes to the further diversity of insect body plans?
● Changes in gene function
● Changes in gene sequence
● Changes in gene expression
➔ Location ➔ Timing
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Bicoid ● Required for head development in
Drosophila and other higher dipterans
Anterior Posterior
➔ Anterior-to-posterior concentration gradient ➔ Maternal effect gene → mRNA comes from
mother
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Bicoid ● Many other insects use other genes for
anterior patterning
Rosenberg, M., Lynch, J., & Desplan, C. (2009). Heads and Tails: Evolution of Antero-Posterior Patterning in Insects. Biochimica et Biophysica Acta, 1789(4), 333–342.
➔ bcd gene is absent from these insects ➔ Bcd has a role in higher flies that was
previously accomplished by other genes
● Then, how did bcd come about?
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Bicoid ● Comes from duplication of Hox3/zen in
cyclorrhaphan flies in the past 140 million years and gained a new function ➔ Neofunctionalization: one copy of a gene takes on an
entirely new function after a duplication event
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Hox3 ● Lost the original function of specifying
segmental identity ➔ Required for extraembryonic tissue development in
winged insects
● Duplicated in higher dipterans
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Hox3 ● D. melanogaster has
two types of Hox3
Stauber, M., Prell, A., & Schmidt-Ott, U. (2002). A single Hox3 gene with composite bicoid and zerknüllt expression characteristics in non-Cyclorrhaphan flies. Proceedings of the National Academy of Sciences of the United States of America, 99(1), 274–279.
➔ zerknüllt (zen): still produces extra- embryonic tissue
➔ bicoid: required for
embryonic patterning
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Discussion Question How could the bcd gene take over the function of another protein and still allow for a healthy animal to exist and function?
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Discussion Question How might the studies of Hox genes in insects such as Drosophila affect our knowledge of vertebrate development or even modern medicine?
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Conclusion ● Hox genes are important for development
➔ Changes in Hox gene expression and function underlie morphological diversity in all of nature
● Ubx facilitates one of the most basic features of the insect body plan: no abdominal appendages
● Many other Hox genes create further diversity among insect body plans