INNOVIVE PERFORMANCE REPORT Innorack 3.0 Acoustic Noise Levels Report · 2020. 3. 13. · Innovive...
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Innorack ® 3.0 Acoustic Noise Levels Report (page 1 of 6) Innovive Inc Test Location I. INTRODUCTION In an animal laboratory environment, noise generated by equipment may have adverse effects on laboratory mice and can ultimately compromise the validity of an experiment. Hearing sensitivity at a range of frequencies is different across species. Mouse hearing ranges from 1 to 100 kHz while human hearing ranges 20 Hz to about 20 kHz. FIGURE 1 shows the human and mouse audiograms with decibels plotted against increasing frequencies. 1 The audiogram shows low hearing sensitivity at low frequencies, with sensitivity increasing as frequency increases until hearing again becomes less sensitive with further frequency increase. Hearing is most sensitive for humans at approximately 1 to 4 kHz and16 kHz for mice. This study was performed to evaluate the sound levels produced by the Innorack ® 3.0 IVC Mouse and to determine if mice are exposed to prolonged high-decibel sound within their hearing range. II. EXPERIMENTAL PROCEDURE Test Location and Equipment This test was performed at the Brüel & Kjær Acoustic Testing Facility in Canton, MI inside a hemi-anechoic chamber. Sound measurements were taken with a B.K.S.V. ¼ inch high frequency microphone. B.K.S.V. Pulse Analyzer software was used to capture and process test data. The test parameters are shown in FIGURE 2. A single-sided, 8-column, 96-cage position Innorack ® 3.0 served as the test IVC rack (see IMAGE 1). The rack was fully populated with cages. Each test cage contained ½ inch of corn cob bedding and a water bottle. Sampling Rate Lines dF Frequency Range Weighting 3.815 us 800 128 Hz 0 – 100 kHz linear FIGURE 1. Human and Mouse Audiograms HUMAN AND MOUSE AUDIOGRAMS Sound Pressure Level (dB/20μPa) 0 10,000 20,000 30,000 40,000 Hz 50,000 60,000 70,000 80,000 90,000 160 140 120 80 100 60 20 40 0 Human Mouse FIGURE 2. Signal Analyzer Test Settings IMAGE 1. Innorack ® 3.0 Mouse in a hemi-anechoic chamber at the Brüel & Kjær Acoustic Teting Facility. INNOVIVE PERFORMANCE REPORT Innorack ® 3.0 Acoustic Noise Levels Report
Transcript of INNOVIVE PERFORMANCE REPORT Innorack 3.0 Acoustic Noise Levels Report · 2020. 3. 13. · Innovive...
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Innorack® 3.0 Acoustic Noise Levels Report (page 1 of 6)Innovive Inc
Test Location
I. INTRODUCTION
In an animal laboratory environment, noise generated by equipment may have adverse effects on laboratory mice and can ultimately
compromise the validity of an experiment. Hearing sensitivity at a range of frequencies is different across species. Mouse hearing ranges
from 1 to 100 kHz while human hearing ranges 20 Hz to about 20 kHz. FIGURE 1 shows the human and mouse audiograms with decibels
plotted against increasing frequencies.1
The audiogram shows low hearing sensitivity at low frequencies, with sensitivity increasing as frequency increases until hearing again
becomes less sensitive with further frequency increase. Hearing is most sensitive for humans at approximately 1 to 4 kHz and16 kHz
for mice. This study was performed to evaluate the sound levels produced by the Innorack® 3.0 IVC Mouse and to determine if mice are
exposed to prolonged high-decibel sound within their hearing range.
II. EXPERIMENTAL PROCEDURE
Test Location and Equipment
This test was performed at the Brüel & Kjær Acoustic Testing
Facility in Canton, MI inside a hemi-anechoic chamber.
Sound measurements were taken with a B.K.S.V. ¼ inch high
frequency microphone. B.K.S.V. Pulse Analyzer software was
used to capture and process test data. The test parameters are
shown in FIGURE 2. A single-sided, 8-column, 96-cage position
Innorack® 3.0 served as the test IVC rack (see IMAGE 1). The rack
was fully populated with cages. Each test cage contained ½ inch
of corn cob bedding and a water bottle.
Sampling Rate
Lines dFFrequency
RangeWeighting
3.815 us 800 128 Hz 0 – 100 kHz linear
FIGURE 1. Human and Mouse Audiograms
HUMAN AND MOUSE AUDIOGRAMS
So
und
Pre
ssur
e Le
vel (
dB
/20µ
Pa)
0 10,000 20,000 30,000 40,000
Hz
50,000 60,000 70,000 80,000 90,000
160
140
120
80
100
60
20
40
0
Human
Mouse
FIGURE 2. Signal Analyzer Test Settings
IMAGE 1. Innorack® 3.0 Mouse in a hemi-anechoic chamber at the Brüel &
Kjær Acoustic Teting Facility.
INNOVIVE PERFORMANCE REPORT
Innorack® 3.0Acoustic Noise Levels Report
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Innorack® 3.0 Acoustic Noise Levels Report (page 2 of 6)Innovive Inc
III. RESULTS
FIGURES 4-10 show test results for each ACH/DP setting used in the test method above.
Test Method
1. Place the microphone inside a disposable mouse cage.
Locate the tip of the microphone in the center of the
cage approximately 1” above the bedding material.
2. Acquire a sound reading with the system off for 30
seconds to get a baseline reading.
3. Insert the cage to rack position A1 (see FIGURE 3).
4. Set the Innorack system to 50 ACH, POS differential
pressure
5. Acquire a sound reading for 30 seconds.
6. Set the Innorack system to 60 ACH, POS differential
pressure.
7. Acquire a sound reading for 30 seconds.
8. Set the Innorack system to 70 ACH, POS differential
pressure.
9. Acquire a sound reading for 30 seconds.
10. Repeat steps (4) through (9) with the cage at positions
A12, D6, D7, E6, E7, H1, and H12 (see FIGURE 3).
11. Repeat steps (3) through (9) with the Innorack system
at 50 ACH, 60 ACH, and 70 ACH at NEG differential
pressure.
1k 1.5k 2k 2.5k 3k 3.5k 4k 4.5k 5k[Hz]
0
4
8
12
16
20
24
28
[dB(A)/200µ Pa]
Background Noise, Rack Interior
A1 - BLUE
A12 - PINK
D6 - GREEN
D7 - YELLOW
E6 - RED
E7 - ORANGE
H1 - LIGHT GREEN
H12 - BLACK
RO
WS
SUPPLY
BLOWER
EXHAUST
BLOWER
COLUMN A B C D E F G H
1 A1 H1
2
3
4
5
6 D6 E6
7 D7 E7
8
9
10
11
12 A12 H12
FIGURE 3. Test Shelf Positions
A1, A12, D6, D7, E6, E7, H1, and H12 represent cage positions
used for testing.
FIGURE 4. Background Noise, Rack Interior
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1k 1.5k 2k 2.5k 3k 3.5k 4k 4.5k 5k[Hz]
0
4
8
12
16
20
24
28
[dB/200µ Pa]
50 ACH, NEG D.P., Rack Interior
1k 1.5k 2k 2.5k 3k 3.5k 4k 4.5k 5k[Hz]
0
4
8
12
16
20
24
28
[dB/200µ Pa]
50 ACH, POS D.P., Rack Interior
A1 - BLUE
A12 - PINK
D6 - GREEN
D7 - YELLOW
E6 - RED
E7 - ORANGE
H1 - LIGHT GREEN
H12 - BLACK
A1 - BLUE
A12 - PINK
D6 - GREEN
D7 - YELLOW
E6 - RED
E7 - ORANGE
H1 - LIGHT GREEN
H12 - BLACK
FIGURE 5. 50 ACH, POS DP, Rack Interior
FIGURE 6. 50 ACH, NEG DP, Rack Interior
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Innorack® 3.0 Acoustic Noise Levels Report (page 4 of 6)Innovive Inc
1k 1.5k 2k 2.5k 3k 3.5k 4k 4.5k 5k[Hz]
0
4
8
12
16
20
24
28
[dB/200µ Pa]
60 ACH, POS D.P., Rack Interior
1k 1.5k 2k 2.5k 3k 3.5k 4k 4.5k 5k[Hz]
0
4
8
12
16
20
24
28
[dB/200µ Pa]
60 ACH, NEG D.P., Rack Interior
A1 - BLUE
A12 - PINK
D6 - GREEN
D7 - YELLOW
E6 - RED
E7 - ORANGE
H1 - LIGHT GREEN
H12 - BLACK
A1 - BLUE
A12 - PINK
D6 - GREEN
D7 - YELLOW
E6 - RED
E7 - ORANGE
H1 - LIGHT GREEN
H12 - BLACK
FIGURE 7. 60 ACH, POS DP, Rack Interior
FIGURE 8. 60 ACH, NEG DP, Rack Interior
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Innorack® 3.0 Acoustic Noise Levels Report (page 5 of 6)Innovive Inc
1k 1.5k 2k 2.5k 3k 3.5k 4k 4.5k 5k[Hz]
0
4
8
12
16
20
24
28
32
3640
[dB/200µ Pa]
70 ACH, POS D.P., Rack Interior
1k 1.5k 2k 2.5k 3k 3.5k 4k 4.5k 5k[Hz]
0
4
8
12
16
20
24
28
[dB/200µ Pa]
70 ACH, NEG D.P., Rack Interior
A1 - BLUE
A12 - PINK
D6 - GREEN
D7 - YELLOW
E6 - RED
E7 - ORANGE
H1 - LIGHT GREEN
H12 - BLACK
A1 - BLUE
A12 - PINK
D6 - GREEN
D7 - YELLOW
E6 - RED
E7 - ORANGE
H1 - LIGHT GREEN
H12 - BLACK
FIGURE 10. 70 ACH, NEG DP, Rack Interior
FIGURE 9. 70 ACH, POS DP, Rack Interior
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Innorack® 3.0 Acoustic Noise Levels Report (page 6 of 6)Innovive Inc
IV. DISCUSSION
Plots were scaled to account for noise occurring only within the mouse hearing range, 1kHz – 100kHz. Cages docked in the top row
recorded highest noise levels, though none exceeded 40dB and occurred at the bottom end of the mouse hearing range. Cages docked
in all other positions recorded 12dB less on average than those docked in the top row.
For all test positions, less than 15dB was present past 2000 Hz with no noise present past 5000 Hz. It can be concluded that acoustic
noise produced by the Innorack® IVC Mouse 3.0 system is not harmful to the well-being of mice inside the cages.
REFERENCES
1. Randall R, Kinard W, Degraff J, Leverage N, Norton J. 2010. Noise in a Laboratory Animal Facility from Human and Mouse
Perspectives. Journal of the American Association for Laboratory Animal Science.
Test ParameterHighest dB
Level*
Frequency
at Highest dB
Frequency Range
of Noise Content
Mic Position
at Highest dB Level
50 ACH, POS DP 23 1000 Hz 1000 – 4600 Hz A1
50 ACH, NEG DP 29 1150 Hz 1000 – 3800 Hz A1
60 ACH, POS DP 27 1150 Hz 1000 – 4750 Hz A1
60 ACH, NEG DP 23 1150 Hz 1000 – 4750 Hz A1
70 ACH, POS DP 38 1024 Hz 1000 – 5000 Hz H1
70 ACH, NEG DP 29 1408 Hz 1000 – 4750 Hz A1
*dB Reference - Background Noise - dB/200µ Pa
FIGURE 11. Summary of Acoustic Data Source: Brüel & Kjær North America Inc.
