The combined application of FTIR microspectroscopy and ToF ...
Innovative technologies for polymer...
Transcript of Innovative technologies for polymer...
Innovative technologies for polymeridentification
Jennifer M. Lynch
1 nm 1 um 1 mm 1 m 1 kmNanoplastics MegaplasticsMeso MacroplasticsMicroplastics
Confirm that particles are plastic polymers
Determine the composition of different polymers in a mixed sample
Importance of polymer identification
PVC
Chemistry
PVC
Uses
PVC
% of U.S. Recycling
PVC
Stratification in ocean
PVC
Stratification in ocean
1 nm 1 um 1 mm 1 m 1 kmNanoplastics MegaplasticsMeso MacroplasticsMicroplastics
By hand
Identify polymers
Chemical/PhysicalChallenging / Unknown
Extract & Purify
Polymer Science (balance, FT-IR, DSC)
Microspectroscopy(µFTIR, µRaman)
Analytical chemistry (pyrolysis GC/MS)
TBD, Promising: AFM-IR, Microfluidic fast Raman, or
pyrolysis GC/MS?
By eyeMicroscopeScanning electron microscope
Visualize
0 10 20 30 40 50
Density separationNile red fluorescence
spectroradiometryXPS
HT-SECDSC
Pyrolysis GC/MSRaman microscopy
FT-IR mode unknownTransmission FT-IR
ATR FT-IR
% of studies
0 50 100 150 200
Density separationNile red fluorescence
spectroradiometryXPS
HT-SECDSC
Pyrolysis GC/MSRaman microscopy
FT-IR microscopyTransmission FT-IR
ATR FT-IR
Instrument cost ($1000)
??
included with ATRRelatively cheap
0 100 200 300 400 500
Density separationNile red fluorescence
spectroradiometryXPS
HT-SECDSC
Pyrolysis GC/MSRaman microscopy
FT-IR microscopyTransmission FT-IR
ATR FT-IR
Analysis time (min)
??
Fast
Open Specy• Free, online• Open source • Spectral analysis tool
• For the microplastics research community
• A growing library of FT-IR and Raman spectra
• Allows users to view, clean, identify, and share their spectra
Website: www.openspecy.org
Website: www.openspecy.org
If interested, email [email protected]
HPU ACC polymer kit PROPOSAL
55
60
65
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75
80
85
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100
5001000150020002500300035004000
% T
Wavenumber (cm-1)
ATR FT-IR spectrum
DSC melt curve
+
1 nm 1 um 1 mm 1 m 1 kmNanoplastics MegaplasticsMeso MacroplasticsMicroplastics
By hand
Identify polymers
ChemicalChallenging / Unknown
Extract & Purify
Polymer Science (balance, FT-IR, DSC)
Microspectroscopy(µFTIR, µRaman)
Analytical chemistry (pyrolysis GC/MS)
TBD, Promising: AFM-IR, Microfluidic fast Raman, or
pyrolysis GC/MS?
By eyeMicroscopeScanning electron microscope
Visualize
1 nm 1 um 1 mm 1 m 1 kmNanoplastics MegaplasticsMeso MacroplasticsMicroplastics
By hand
Identify polymers
Extract & Purify
Polymer Science (balance, FT-IR, DSC)
By eye
Visualize
1 mm to megaDon’t trust 1377 cm-1 band to differentiate LDPE from HDPE
Benefits of Differential Scanning Calorimetry
LLDPE: 124 oCLDPE: 106 oC HDPE: 135 oC
Differentiate polyethylene densities
Even on particles as small as 0.03 mg (~100 µm)
LDPE vs. HDPE differentiation
• 16 debris pieces had the 1377 cm-1 band in ATR FT-IR spectra = LDPE
• ATR FT-IR is not reliable in differentiating weathered PE
• Weathering affects ATR FT-IR spectra
0
20
40
60
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Mild Moderate Severe%
corr
ect b
y AT
R FT
IR
Extent of Weathering
DSC Confirmation of ATR FT-IR's LDPE
N=4 N=7 N=5
Four-step workflow
1. ATR FT-IR
2. FT-IR total transmission of thin film
3. Differential Scanning Calorimetry
4. Optical or FT-IR microscopy of cross sections
12
4 3
example – Four-Step workflow
1. ATR FT-IR
2. FT-IR total transmission of thin film
3. Differential Scanning Calorimetry
4. Optical or FT-IR microscopy of cross sections
Inner layer is EVA; outer layer is PS
0.45 mils (EVA-PE) 0.16 mils (PE) 0.40 mils (EVOH) 0.28 mils (EVA-tie)
100x
1 mm to mega
Four-Step workflow revealed…
•63 % of HI debris items were made of multiple polymers
•73% of those were not revealed by ATR FT-IR alone
•Multiple methods are required to reveal multi-layer, multi-polymer items, & differentiate PEs
1 mm to mega
1 nm 1 um 1 mm 1 m 1 kmNanoplastics MegaplasticsMeso MacroplasticsMicroplastics
Identify polymers
Physical/Chemical
Extract & Purify
Microspectroscopy
Microscope
Visualize
AbioticBeach Sand
Collection
Extraction
Purification
Separation
Analysis
micro
Quadrat and shovel
Collection
Extraction
Purification
Separation
Analysis
Buoyancy Separation Device (BSD)
2 buckets of sand2 buckets of seawater
micro
www.seed.world
Collection
Extraction
Purification
Separation
Analysis
Buoyancy Separation Device (BSD)micro
Clean Sand
Plastic & Plant
Collection
Extraction
Purification
Separation
Analysis
Trash Time Machine (TTM) aka Debris Separation Vacuum Chamber
micro
Plastic & Plant Plastic
Plant
www.seed.world
Collection
Extraction
Purification
Separation
Analysis
Sieves
>4 mm
> 4 mm 2 - 4 mm
1 -2 mm 0.5 – 1 mm
0.25 – 0.45 mm 0.125 – 0.25mm
0.063 – 0.125 mm <63 µm
Analysis- Tiny (63 µm)Collection
Extraction
Purification
Separation
Analysis
micro
Analysis- Tiny (63 µm)Collection
Extraction
Purification
Separation
Analysis
Plastic
micro
Collection
Extraction
Purification
Separation
Analysis
Analysis- Tiny (63 µm)
PPPENylonnatural polyamides
p ycelluloserubberCarbonatesOther
Plastic
Plant
Sand
micro
1 nm 1 um 1 mm 1 m 1 kmNanoplastics MegaplasticsMeso MacroplasticsMicroplastics
Identify polymers
Microdissection/Chemical
Extract & Purify
Microspectroscopy(µFTIR, µRaman)
Microscope
Visualize
BioticLarval fish
Photos: Jon Whitney, Frank Baensch, Cedric Guigand Gove Whitney et al. 2019 PNAS 116:24143-24149
micro
Polyester
Raman
FT-IR
Gove Whitney et al. 2019 PNAS 116:24143-24149
micro
Jenna Karr
Chemical storage and digestion methods: Are they compatible with polymers?
Jenna Karr
micro
Experimental Design: After Plastic + FilterIn tin tray
Vacuum Pump
Sample Set
Before & AfterMassCountSizeFT-IRPhoto
Experimental Design
Storage95% EtOH1 week1 month
DigestionHNO3KOHFenton’sBleachSonication
micro
microCompatibility Chart
1 nm 1 um 1 mm 1 m 1 kmNanoplastics MegaplasticsMeso MacroplasticsMicroplastics
Identify polymers
Challenging / Unknown
Extract & Purify
TBD, Promising: AFM-IR, Microfluidic fast Raman, or
pyrolysis GC/MS?
Scanning electron microscope
Visualize
NIST Nanoplastics PROPOSAL1. Produce nanoplastic materials of different polymers
• NanoFAB• SPHERE• Possibly with fluorescent or mass-labeled tags
2. Test methods to detect, characterize, or quantify nanoplastics• BCARS• Pyrolysis-GC/MS
3. Test methods to separate, purify, or concentrate nanoplastics• Drinking water• Beach sand
4. Perform in vitro toxicology studies
nano
1. Produce nanoplastic materials of different polymers• NanoFAB• SPHERE• Possibly with fluorescent or mass-labeled tags
2. Test methods to detect, characterize, or quantify nanoplastics• Vector-scan coherent-Raman confocal microspectroscopy• Pyrolysis-GC/MS
3. Test methods to separate, purify, or concentrate nanoplastics• Drinking water• Beach sand
4. Perform in vitro toxicology studies
NIST Nanoplastics PROPOSALnano
1. Produce nanoplastic materials of different polymers• NanoFAB• SPHERE• Possibly with fluorescent or mass-labeled tags
2. Test methods to detect, characterize, or quantify nanoplastics• BCARS• Pyrolysis-GC/MS
3. Test methods to separate, purify, or concentrate nanoplastics• Drinking water• Beach sand
4. Perform in vitro toxicology studies
NIST Nanoplastics PROPOSALnano
1. Produce nanoplastic materials of different polymers• NanoFAB• SPHERE• Possibly with fluorescent or mass-labeled tags
2. Test methods to detect, characterize, or quantify nanoplastics• BCARS• Pyrolysis-GC/MS
3. Test methods to separate, purify, or concentrate nanoplastics• Drinking water• Beach sand
4. Perform in vitro toxicology studies
NIST Nanoplastics PROPOSALnano
Thank you!
• John Kucklick, Kate Beers, Sara Orski, Katy Shaw, Sam Stavis, Li Pinn Sung, A. Pintar, B. Toman, Charlie Camp, Elijah Peterson
• Melissa Jung, Kayla Brignac, Jenna Karr, Brenda Jensen, David Hyrenbach, Keith Korsmeyer
• Rachel Sandquist, Jon Whitney, Jamie Gove, Jana Phipps
• Wanda Weatherford, David Springer, Taanya Ramirez, Ron Abbott, Rick Wagner
• Bridget O’Donnell
• Suja Sukumaran, Matt Cerutti, Brian Bertsch
• Ray Aivazian, Jim Potemra, Anupam Misra, Lloyd Hihara, Jan Kealoha
• Dipak Mainali, Darren Robey
• Brett Howard
• Win Cowger, Andrew Gray, H. Hapich, Chelsea Rochman, Sebastian Primpke, O. Herodotou
Follow us
Website: www.hpu.edu/cncs/cmdr
CENTER FOR MARINE DEBRIS RESEARCH
@MarineDebrisResearch
@debrisresearch
@company/center-for-marine-debris-research