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Influenza Branch VACCINES AND RELATED BIOLOGICAL PRODUCTS ADVISORY COMMITTEE CBER, FDA February...
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Transcript of Influenza Branch VACCINES AND RELATED BIOLOGICAL PRODUCTS ADVISORY COMMITTEE CBER, FDA February...
Influenza BranchInfluenza Branch
VACCINES AND RELATED BIOLOGICAL PRODUCTS ADVISORY COMMITTEE
CBER, FDA
February 16-17, 2005, Bethesda, MD
Nancy COX, Ph.D.
WHO COLLABORATING CENTER FOR SURVEILLANCE,EPIDEMIOLOGY AND CONTROL OF INFLUENZA
Influenza BranchDivision of Viral and Rickettsial DiseasesNational Center for Infectious Diseases
Centers for Disease Control and PreventionAtlanta, GA 30333
Influenza BranchInfluenza Branch
Influenza A(H5N1)December 2003 – February 2005
H5N1 –outbreaks in birds reported
H5N1 – outbreaks in birds and humans
H5N1 –outbreaks in birds , controlled and final report submitted to OIE
Influenza BranchInfluenza Branch
Human H5N1 Cases, 2004-Human H5N1 Cases, 2004-20052005
CountryCountry H5N1 CasesH5N1 Cases DeathsDeaths Case Case FatalityFatality
CambodiaCambodia 11 11 100%100%
ThailandThailand 1717 1212 71%71%
VietnamVietnam 3737 2929 78%78%
TotalTotal 5555 4242 76%76%
High case fatality regardless of age Clinical symptoms similar to earlier cases, including lymphopenia Diarrhea prominent in some casesSecond wave of infections in Aug 2004-presentThai family cluster; probable H-to-H transmission
Influenza BranchInfluenza Branch
320320160160
80802020
6406408080
808012801280
4040640640
4040640640
HK/213/03HK/213/03
CK/KOR/ES/03CK/KOR/ES/03
HK/97HK/97
TH/353/04TH/353/04
VN/1203/04VN/1203/04
VN/1194/04VN/1194/04
51205120
2020
320320
NDND
2020
<10<10
HK/213/03HK/213/03CK/KO/03CK/KO/03
320320
4040
320320
<10<10
<10<10
<10<10
HK/97HK/97VN/1203/03VN/1203/03
Antigenic Characterization of H5N1 Antigenic Characterization of H5N1 Influenza VirusesInfluenza Viruses
VIRUSES FERRET H5N1 ANTISERA
Influenza BranchInfluenza Branch
Avian influenza viruses can pose a major risk to global public Avian influenza viruses can pose a major risk to global public health (H5N1, H7N7, H9N2, etc.)health (H5N1, H7N7, H9N2, etc.)
Early detection H-2-H transmission of novel influenza viruses Early detection H-2-H transmission of novel influenza viruses is essentialis essential
2003/05 Asian viruses are heterogeneous in AV susceptibility 2003/05 Asian viruses are heterogeneous in AV susceptibility and pathogenicity; more lethal in mammals than 1997 strainsand pathogenicity; more lethal in mammals than 1997 strains
Ongoing vaccine development, antiviral stockpiling and Ongoing vaccine development, antiviral stockpiling and pandemic preparednesspandemic preparedness
Surveillance in animals (birds, swine and other susceptible Surveillance in animals (birds, swine and other susceptible hosts) is critical hosts) is critical
Research agenda needed to enhance our understanding of Research agenda needed to enhance our understanding of the genesis of pandemic influenza virusesthe genesis of pandemic influenza viruses
Global recognition of need for better communication Global recognition of need for better communication between human and veterinary health authoritiesbetween human and veterinary health authorities
ConclusionsConclusions
Influenza BranchInfluenza Branch
Influenza: An Emerging Disease that Influenza: An Emerging Disease that Rolls OnRolls On
Influenza BranchInfluenza Branch
Influenza Virus Isolates Characterized by CDC and Other WHO Collaborating Centers and Laboratories *
(October 2004 – February 2005)
North
America Europe Asia
Cent/So America
Africa, Oceania
Total (%)
A(H1N1) 2 115 74 2 193 (8%)
A(H3N2) 616 402 212 4 458 1692 (75%)
B/Yamagata 98 62 30 1 112 303 (14%)
B/Victoria 18 28 16 3 9 74 (3%)
Total 734 607 332 8 581 2262 Preliminary Data 02/14/2005
* Data based on cumulative influenza virus isolates antigenic characterization data provided by WHO Collaborating Centers in Atlanta, London, Melbourne, Tokyo, reports from European Influenza Surveillance Scheme and National Influenza Center, Canada
Influenza BranchInfluenza Branch
Influenza A(H1N1)
Influenza BranchInfluenza Branch
HEMAGGLUTINATION INHIBITION REACTIONS OF INFLUENZA H1 VIRUSESREFERENCE FERRET ANTISERA
DateREFERENCE ANTIGENS BJ/262 NC/20 FUJ/156 NEI/52 CI/8885 PE/3135 collected Passage
1 A/BEIJING/262/95 640 640 160 320 320 40 01/09/95 E3/E32 A/N. CALEDONIA/20/99 * 160 640 320 320 320 160 06/09/99 E4/E23 A/FUJIAN/156/2000 80 640 320 320 160 80 08/25/00 E2/E24 A/NEIMENGGU/52/2002 40 320 160 320 160 40 01/25/02 E2/E45 A/CHILE/8885/2002 40 320 160 160 320 20 07/03/02 E1/E46
7
A/PERU/3135/2003 160 40 80 20 10 640 04/09/03 X/C3
TEST ANTIGENS
8A/FLORIDA/04/2004 * 320 1280 1280 1280 640 320 12/20/04 X1/C1
9A/FLORIDA/03/2004 320 1280 640 640 320 320 12/09/04 X2/C1
10A/PRACHINBURI/1749/2004 320 1280 1280 1280 320 640 09/18/04 C1/C1
11A/BANGKOK/1757/2004 320 1280 1280 1280 640 640 09/20/04 C1/C1
12A/PRACHINBURI/1817/2004 320 1280 1280 1280 640 640 09/23/04 C1/C1
13A/BANGKOK/1876/2004 320 1280 1280 1280 640 320 09/27/04 C1/C1
14A/SUPHANBURI/2131/2004 320 1280 1280 1280 640 320 10/04/04 C1/C1
15A/BANGKOK/1752/2004 320 1280 640 1280 320 320 09/20/04 C1/C1
16A/SUPHANBURI/2130/2004 160 1280 640 1280 320 320 10/04/04 C1/C1
17A/BANGKOK/1822/2004 160 1280 640 1280 320 320 09/23/04 C1/C1
18A/RATCHABURI/1847/2004 320 1280 640 640 320 320 09/24/04 C1/C1
19A/BANGKOK/1544/2004 * 160 640 320 640 320 160 08/31/04 E3/E1
20A/SUPHANBURI/2129/2004 160 640 640 640 320 160 10/04/04 C1/C1
21A/SAKAEW/2132/2004 160 640 320 640 320 320 10/04/04 C1/C1
22A/SINGAPORE/14/2004 160 1280 640 640 320 160 03/31/04 E4/E1
23A/N. CALEDONIA/9/2004 160 1280 640 640 320 320 06/11/04 E2/E1
24A/N. CALEDONIA/3/2004 160 640 640 640 320 160 04/19/04 E2/E1
25A/MALAYSIA/1513/2004 160 640 320 640 160 320 07/10/04 E2/E1
26A/HONG KONG/4555/2004 160 640 640 640 320 160 09/25/04 C2/C1
27A/HONG KONG/4580/2004 160 640 640 640 320 160 10/08/04 C2/C1A/HONG KONG/4596/2004 160 1280 1280 1280 640 320 10/25/04 C2/C1* Serology antigens
Influenza BranchInfluenza Branch
Influenza A(H1) Isolates Characterized by CDC
U.S.A. North America
Europe Asia Cent/So America
Africa, Australia,
New Zealand
Total (%)
October 2003 – March 2004
A/N.Caledonia/20/99- like 3 2 1 16 1 2 25 (9%)
A/N.Caledon./20/99 (low) 2 1 3 (11%)
Total 28
April 2004 – September 2004
A/N.Caledonia/20/99- like 1 4 36 2 43 (98%)
A/N.Caledon./20/99 (low) 1 1 (2%)
44
October 2004 – January 2005
A/N.Caledonia/20/99- like 2 12 14(100%)
A/N.Caledon./20/99 (low)
14
Total 5 3 7 65 2 4 86
Influenza BranchInfluenza Branch
Evolutionary Relationships Among Influenza A(H1) HA Genes
0 5nucleotides
(LR) Low reactor in HI# - Egg Isolate
* Serology Antigen
Hong Kong/2637/04nimrHong Kong/2637/04
nimrHong Kong/2675/04Taiwan/1559/04niidTaiwan/1559/04
ausChrist Church/106/04ausPhilippines/987/04
Taiwan/1571/04ausPhilippines/936/04
niidOkinawa/42/04ausAyathaya/2111/04
Ayathaya/1720/04Bangkok/1544/04#*ausTaiwan/1559/04
ausBangkok/1940/04Canada/591/04
Singapore/11/2004Massachusetts/1/04
Singapore/5/04ausNew Caledonia/3/04#
ausNew Caledonia/4/04#ausNew Caledonia/9/04#
Indonesia/5840/04Indonesia/5841/04
Singapore/14/04#*ausSingapore/14/04#*
Florida/3/04nimrIreland/15680/04
Florida/4/04*nimrNetherlands/128/04*
twTaiwan/1574/04twTaiwan/1573/04
niidOkayama/53/04niidNagano/1328/03
ausPrachinburi/1686/04Sakaew/2132/04nimrIreland/9728/04Hong Kong/4596/04
Prachinburi/1817/04Nonthaburi/1739/04
ausMalaysia/1513/04#nimrLyon/Chu28824/04
nimrLyon/Gi346/04Saudi Arabia/2526/04
Suphanburi/2129/04Ratchaburi/1847/04New Caledonia/20/99#*V169A, N190D, W255R
V317AY256F
F256YA317V
V317A
R149K
Q285H
T90K, R212KT269N
Y101H
R192K
Jan-Feb-MarApr-May-JunJul-Aug-SepOct-Nov-Dec
Date Collected
Influenza BranchInfluenza Branch
Evolutionary Relationships Among Influenza N1 NA Genes
Nucleotides
*Serology antigen(LR) Low reaction to HI
# Egg isolate
0 Scale 3
A/Suphanburi/2129/2004 A/Hong Kong/4555/2004 A/Bangkok/1724/2004 A/Bangkok/1752/2004
A/Bangkok/406/2004# A/Bangkok/1876/2004
A/Bangkok/1507/2004# A/Bangkok/1759/2004
A/Bangkok/1498/2004# A/Bangkok/1757/2004 A/Prachinburi/1817/2004 A/Prachinburi/1749/2004 A/Bangkok/1822/2004
A/Bangkok/1467/2004# A/Nonthaburi/1738/2004
A/Bangkok/1410/2004# A/Nonthaburi/1739/2004 A/Florida/03/2004
A/Florida/04/2004* A/Suphanburi/2130/2004 A/Suphanburi/2131/2004
A/Bangkok/1755/2004 A/Hong Kong/4580/2004
A/Nagano/1328/2003 A/Argentina/678/2003
A/New Mexico/7/2003 aus-A/Malaysia/1003/2003 A/Malaysia/1513/2004# A/Mexico/268/2003
A/Romania/955/2003 nimr-A/Moscow/7/2003 aus-A/Chrischur/1/2003
nimr-A/Israel/2/2003 A/Michigan/5/2003
A/Michigan/4/2003 A/Michigan/6/2003 A/Michigan/1/2003
nimr-A/Barcelona/7891v31/2003 A/Peru/2223/2003 A/Peru/3192/2003
A/Peru/3135/2003 A/Minnesota/18/2003
A/Gansu/185/2003 aus-A/Darwin/1/2003
A/New Caledonia/3/2004# A/Singapore/14/2004
A/Sakaew/2132/2004 A/Singapore/14/2004#*
A/Singapore/23/2004 A/Ayatthaya/2111/2004
A/Bangkok/1544/2004#* A/Bangkok/1699/2004
A/Bangkok/1741/2004 A/Bangkok/1742/2004
A/Hong Kong/2637/2004 A/Ratchaburi/1847/2004
A/Bangkok/1468/2004# A/Ayutthaya/1720/2004
A/Guangzhou/78/2003 A/Sichuan/646/2002
A/Argentina/6/2003 A/New Caledonia/20/99
E332KN450D
V234M
E214G, R222QD382N
Influenza BranchInfluenza Branch
HI Antibody Response to 2004-2005 Vaccine(adults)
% w/HI titer >40
Sera N Antigen %
Rise Pre GMT
Post GMT
Pre Vaccine
Post Vaccine
30 A/New Caledonia/20/99 23 21 44 43 67 30 A/Bangkok/1544/2004 17 26 54 53 77
30 A/Florida/04/2004 17 27 54 53 73
Japan
30 A/Netherlands/128/2004 27 27 59 57 77
25 A/New Caledonia/20/99 32 19 54 44 76 25 A/Bangkok/1544/2004 32 23 57 40 76
25 A/Florida/04/2004 24 24 53 48 80
USA
25 A/Netherlands/128/2004 24 26 66 52 80
Influenza BranchInfluenza Branch
H1 Summary: October 2004 to February 2005H1 Summary: October 2004 to February 2005
Relatively few H1 viruses have been detected worldwideRelatively few H1 viruses have been detected worldwide
The majority of H1N1 viruses were closely related The majority of H1N1 viruses were closely related antigenically to the A/New Caledonia/20/99 vaccine strain and antigenically to the A/New Caledonia/20/99 vaccine strain and no significant variants of H1N1 viruses were detectedno significant variants of H1N1 viruses were detected
No H1N2 viruses were detectedNo H1N2 viruses were detected
N1 neuraminidase genes of recent H1 viruses were similar to N1 neuraminidase genes of recent H1 viruses were similar to those of viruses isolated prior to October 2004those of viruses isolated prior to October 2004
Influenza BranchInfluenza Branch
Influenza A(H3N2)
Influenza BranchInfluenza Branch
HEMAGGLUTINATION INHIBITION REACTIONS OF INFLUENZA H3 VIRUSESSTRAIN DESIGNATION REFERENCE FERRET ANTISERA
DateREFERENCE ANTIGENS WY/3 WEL/01 ND/01 CA/7 SN/37 collected Passage
1 A/WYOMING/03/2003 X-147 * 640 80 160 160 40 02/13/03 X/E12 A/WELLINGTON/01/2004 * 160 320 320 320 320 01/26/04 E3/E23 A/NORTH DAKOTA/01/2004 80 160 640 320 160 10/20/04 SpfCK1E44 A/CALIFORNIA/07/2004 * 160 80 320 320 160 09/16/04 SpfCK1E3
6
7
5 A/SINGAPORE/37/2004 160 160 640 640 640 6/7/2004 E4/E1
TEST ANTIGENS
8
A/KENTUCKY/6e/2004 320 320 640 1280 1280 11/27/04 SpfC1KE3
9
A/NEW YORK/57/2004 320 160 640 640 160 12/28/04 X2/C1
10
A/VIRGINIA/02/2005 320 320 1280 640 640 01/06/05 M1/C1
11
A/WISCONSIN/21e/2004 160 160 320 320 160 11/29/04 SpfCK3E3
12
A/NEW YORK/39e/2004 160 160 640 640 320 11/09/04 SpfCK2E4
13
A/NEW YORK/02/2005 160 80 320 320 160 01/05/05 X1/C1
14
A/NEW YORK/03/2005 160 40 320 320 80 01/03/05 X1/C1
15
A/TEXAS/12/2004 160 80 320 320 160 12/29/04 M1/C1
16
A/MASSACHUSETTS/01/2005 160 80 320 640 160 01/05/05 M1/C1
17
A/ALASKA/16/2004 160 80 320 160 80 12/12/04 X/C1
18
A/NEW YORK/55e/2004 80 80 320 320 160 11/18/04 SpfCK2E2
19
A/N. HAMPSHIRE/04/2004 80 40 160 160 160 12/27/04 X/C1
20
A/WISCONSIN/16e/2004 40 80 320 160 80 11/22/04 SpfCK3E2
21
A/WEST VIRGINIA/01/2005 80 40 160 160 40 01/05/05 C1/C1
22
A/VIRGINIA/03/2005 80 80 320 160 80 01/07/05 M1/C1
23
A/ARIZONA/03/2005 80 40 160 160 80 01/05/05 M2/C1
24
A/GEORGIA/01/2005 80 40 160 160 80 01/06/05 M1/C1
25
A/CANADA/707/2004 160 80 320 320 80 11/16/04 X1/C1
26
A/VICTORIA/523/2004 80 160 320 320 640 11/03/04 E5/E1
27
A/MADRID/RR1734/2004 320 160 640 640 320 12/01/04 C1/C1
28
A/UNITED KINGDOM/305/2005 160 80 320 320 80 01/05/05 X/C1
29
A/NORWAY/1103/2004 80 20 80 160 40 11/22/04 C2/C1
30
A/IRAQ/01/2004 80 40 160 160 80 12/29/04 X/C1A/SINGAPORE/68/2004 160 160 160 320 nd 11/09/04 C1/C2A/HONG KONG/4465/2004 80 20 160 160 40 09/15/04 C2/C1
* Serology antigens
Influenza BranchInfluenza Branch
(guinea pig red blood cells)HEMAGGLUTINATION INHIBITION REACTIONS OF INFLUENZA H3 VIRUSES
STRAIN DESIGNATION REFERENCE FERRET ANTISERADate
REFERENCE ANTIGENS WY/3 WEL/01 VIC/500 CA/07 collected Passage
1 A/WYOMING/03/2003 X-147 * 640 40 160 80 02/13/03 X/E12 A/WELLINGTON/01/2004 * 320 640 1280 640 01/26/04 E3/E23 A/VICTORIA/500/2004 40 40 160 160 08/13/04 E6/E24 A/CALIFORNIA/07/2004 * 80 80 160 320 09/16/04 SPpfCK3E3
TEST ANTIGENS
5 A/VERMONT/10/2004 320 160 640 640 12/13/04 X/C26 A/NEW YORK/63/2004 320 80 640 640 12/06/04 X1/C27 A/NEW HAMPSHIRE/09/2004 320 160 640 640 12/13/04 MX/C28 A/NEW HAMPSHIRE/10/2004 320 80 320 160 12/13/04 MX/C29 A/NEW HAMPSHIRE/11/2004 160 160 320 640 12/26/04 MX/C2
10 A/NEW HAMPSHIRE/07/2004 320 80 640 320 12/12/04 MX/C211 A/NEW YORK/62/2004 160 40 320 320 12/06/04 X2/C212 A/VERMONT/11/2004 160 80 320 320 12/08/04 X/C213 A/NEW HAMPSHIRE/05/2004 160 40 320 320 12/09/04 MX/C214 A/NEW HAMPSHIRE/08/2004 160 40 320 320 12/10/04 MX/C215 A/CALIFORNIA/08/2004 80 40 160 160 10/12/04 M2/C216 A/LOUISIANA/02/2004 80 40 160 160 12/15/04 X/C217 A/COLORADO/08/2004 160 80 320 320 12/20/04 MX/C218 A/LOUISIANA/03/2004 160 80 320 320 12/21/04 X/C219 A/WASHINGTON/13/2004 160 80 320 320 12/14/04 X/C220 A/NEW JERSEY/09/2004 160 80 320 640 12/03/04 X/C221 A/PERU/218/2004 80 20 160 160 12/20/04 X/C222 A/PERU/225/2004 80 40 160 160 12/28/04 X/C223 A/IRAQ/29/2004 160 40 160 320 12/21/04 X/C224 A/KOREA/298/2004 160 20 160 320 12/29/04 X/C225 A/HUNAN/L198-5/2004 160 40 320 320 09/06/04 C3/C2
* Serology antigens
Influenza BranchInfluenza Branch
Influenza A(H3N2) Isolates Characterized by CDC
U.S.A. North America
Europe Asia Cent/So America
Africa, Australia,
New Zealand
Total (%)
April 2004 – September 2004
A/Fujian/411/2002-like 29 17 4 143 151 4 348 (82.3%)
A/Fujian/411/2002 (low) 7 45 8 1 61 (14.4%)
A/California/7/2004-like * 11 2 13* (3.1%)
A/Wellington/1/2004-like 1 1 (0.2%)
Total 423
October 2004 – January 2005
A/Fujian/411/2002-like 119 12 2 14 1 1 149 (57%)
A/Fujian/411/2002 (low) 27 4 1 1 33 (12.5%)
A/California/7/2004-like * 59 3 3 9 2 2 78* (30%)
A/Wellington/1/2004-like 1 1 (0.5%)
Total 261
Total 241 36 9 224 162 12 684 * A/California/7/2004 ferret antiserum was used starting from January 5, 2005 Preliminary data 02/09/2005
Influenza BranchInfluenza Branch
Evolutionary Relationships Among Influenza A(H3) HA Genes
0.01
NIID-Kobe/189/2004 Aus-Malaysia/25/2004 LR
Wisconsin/16/2004 # Wisconsin/19/2004 # North Dakota/1/2004 # LR
Georgia/1/2004 LR Texas/6/2004 LR
New York/39/2004 # New York/55/2004
New York/40/2004 # Nepal/1646/2004 LR
NIMR-Latvia/15743/2004
Singapore/36/2004 # LR * Singapore/37/2004 # LR
Aus-Victoria/512/2004 # Maryland/2/2004 LR
Washington/1/2004 # LR NIMR-Lisbon/4/2004
California/7/2004 # LR * Alaska/3/2004 # LR
Aus-Wellington/1/2004 # * El Salvador/43/2004 LR
Japan/120/2004 # Wisconsin/21/2004 #
Guangdong/596/2004 LR Canada/578/2004 LR *
Hong Kong/2874/2004 LR Hunan/L200-3/2004 *
Tennessee/6/2004 LR * NIID-Mie/1/2005
Ningbo/1032/2004 * Aus-Victoria/500/2004 #
NIID-Sendai/67/2004 Hong Kong/1186/2003 # LR
Fujian/411/2002 Korea/770/2002
Texas/40/2003 # Wyoming/3/2003 (X-147) # *
Peru/1296/2004 LR Panama/2007/99 #
LR - Low reactor to A/Wyoming/3/2003
Vaccine Strain# - egg isolate
* - Reference antigen
K 145 N
D 188 N
N 278 K
K 145 NK 326 T
S 227 P
22
Influenza BranchInfluenza Branch
Evolutionary Relationships Among Influenza N2 NA GenesNingbo/982/2004
Ningbo/1043/2004 Ningbo/1042/2004 Ningbo/1032/2004(LR)* Ningbo/1114/2004
Ningbo/1018/2004 Hong Kong/2874/2004 HongKong/2976/2004 HongKong/2982/2004
Hong Kong/2874/2004 Ningbo/1119/2004
Hunan/L200-3/2004(LR)* Ningbo/1049/2004 Tennessee/6/2004(LR)*
N. Dakota/01/2004# nimr-Lisbon/3/2004
NewYork/55/2004# nimr-Baleares/720/2004
nimr-Ireland/9195/2004 Texas/6/2004
Ningbo/997/2004 Ningbo/1001/2004
NewYork/40/2004# Washington/1/2004 California/7/2004(LR)#*
Wellington/1/2004(LR)#* Singapore/15/2004 NewCaledonia/7/2004#
Argentina/126/2004 Brazil/1759/2004
nimr-Reunion/581/2004 Hunan/L201-2/2004
Japan/120/2004# Singapore/21/2004
Singapore/36/2004(LR)#* Nepal/1646/2004
Korea/770/2002 Wyoming/3e/2003#*
Fujian/411/2002 nihj-Nagasaki/52/2003
Canada/578/2004(LR)* Ningbo/1005/2004
Wuhan/269/2004 FrenchGuyana/12/2004
Uruguay/3173/2004 Peru/1296/2004
Victoria/505/2004# Panama/2007/99
Sydney/5/97
Nucleotides
*Serology antigen(LR) Low reactor in HI
# egg isolate
0 Scale 7
K221EI392T
N92D, EI99KQ432E
S18A, F23L, I30V, Y40H, F42CV143G, K172R, V216G, T265I, I307V, N385K, D399E, L437W
Influenza BranchInfluenza Branch
H3 Summary: October 2004 to February 2005 Influenza A (H3N2) viruses circulated in many countries in the Influenza A (H3N2) viruses circulated in many countries in the
Americas, Asia, Europe and Oceania.Americas, Asia, Europe and Oceania.
In HI tests, A(H3N2) viruses were antigenically heterogeneous. In HI tests, A(H3N2) viruses were antigenically heterogeneous. Viruses isolated early in the season were often more closely related Viruses isolated early in the season were often more closely related to A/Fujian/411/02 and A/Wyoming/3/03.to A/Fujian/411/02 and A/Wyoming/3/03.
An increasing proportion of recent isolates were antigenically An increasing proportion of recent isolates were antigenically
distinguishable from the A/Wyoming/3/03 and A/Wellington/1/04 distinguishable from the A/Wyoming/3/03 and A/Wellington/1/04 vaccine strains and were most closely related to the A/California/7/04 vaccine strains and were most closely related to the A/California/7/04 reference virus.reference virus.
Sequence analysis of N2 neuraminidase genes of recent H3N2 Sequence analysis of N2 neuraminidase genes of recent H3N2 viruses indicates that NAs of recent viruses were genetically viruses indicates that NAs of recent viruses were genetically distinguishable from that of the Wyoming/3/03 virus (N93D, E199K, distinguishable from that of the Wyoming/3/03 virus (N93D, E199K, Q432E) but were similar to the NAs of A/Wellington/1/04 and Q432E) but were similar to the NAs of A/Wellington/1/04 and A/California/7/04. A/California/7/04.
Influenza BranchInfluenza Branch
Influenza B
Influenza BranchInfluenza Branch
STRAIN DESIGNATION REFERENCE FERRET ANTISERADate
REFERENCE ANTIGENS SIC/379 SHA/361 JIL/20 JIA/10 FL/7 HK/1434 BRIS/32 HI/13 collected Passage
1 B/SICHUAN/379/99 640 320 80 160 640 20 10 5 12/02/99 E2/E12 B/SHANGHAI/361/2002 640 320 160 160 640 40 5 5 06/12/02 E2/E23 B/JILIN/20/2003 * 320 160 160 160 640 20 5 5 02/21/03 E2/E44 B/JIANGSU/10/2003 80 80 80 1280 320 5 5 5 09/11/03 E3/E25 B/EGYPT/2040/2004 640 320 160 320 1280 20 5 5 04/27/04 C2/C26 B/FLORIDA/07e/2004 * 320 160 80 160 640 20 10 5 10/01/04 E37 B/SHANDONG/07/1997 10 5 5 5 5 640 320 640 11/03/97 E4/E38 B/HONG KONG/1434/2002 10 5 5 5 5 1280 640 640 01/15/02 E59 B/BRISBANE/32/2002 10 5 5 5 10 640 640 640 07/02/02 E2/E4
10 B/HAWAII/13/2004 * 5 5 5 5 5 320 160 320 06/21/04 E2
TEST ANTIGENS
11 B/COLORADO/04e/2004 * 320 320 80 160 640 20 5 5 11/09/04 E212 B/OREGON/01/2005 160 160 80 320 640 20 5 5 01/03/05 R1/C113 B/WEST VIRGINIA/01/2004 160 160 80 320 640 10 5 5 12/28/04 C1/C114 B/WASHINGTON/03/2004 320 320 160 640 640 10 5 5 12/30/04 M1/C115 B/VIRGINIA/01/2005 160 160 80 320 640 20 5 5 01/03/05 M1/C116 B/ARIZONA/01/2005 80 160 80 320 640 10 5 5 01/05/05 M1/C117 B/NEW YORK/27/2004 160 160 80 320 640 20 5 5 12/29/04 M1/C118 B/MISSISSIPPI/02/2005 80 160 80 320 640 10 5 5 01/10/05 X/C119 B/NEVADA/02/2005 160 160 80 640 640 10 5 5 01/16/05 M1/C120 B/TEXAS/06/2004 160 160 80 320 320 20 5 5 12/30/04 M1/C121 B/NORTH DAKOTA/02/2004 160 160 80 640 640 20 5 5 12/29/04 M2/C122 B/NORWAY/1102/204 80 160 80 320 640 10 5 5 11/22/04 C2/C123 B/TURKEY/187/2004 320 160 40 40 320 20 10 5 12/27/04 X/C124 B/FUJIAN/430/2004 * 160 160 80 80 320 10 5 5 09/04/04 E1C2/C1E225 B/NEVADA/01/2005 5 5 5 5 5 160 80 40 01/04/05 X/C126 B/TEXAS/01/2005 5 5 5 5 5 80 40 20 01/10/05 M1/C127 B/HONG KONG/310e/2004 20 20 5 5 20 640 320 640 11/24/04 E228 B/HONG KONG/312/2004 5 5 5 5 5 80 40 40 11/29/04 C2/C129 B/HONG KONG/318/2004 5 5 5 5 5 160 80 40 12/15/04 C2/C130 B/SINGAPORE/15/2004 5 5 5 5 5 320 640 640 11/08/04 C2/C131 B/SINGAPORE/14/2004 5 5 5 5 5 320 320 320 11/02/04 C2/C132 B/SINGAPORE/18/2004 5 5 5 5 5 160 80 40 11/22/04 C2/C133 B/SINGAPORE/08/2004 5 5 5 5 5 80 40 40 08/16/04 C2/C134 B/SINGAPORE/11/2004 5 5 5 5 5 160 40 40 10/08/04 C2/C135 B/SINGAPORE/16/2004 5 5 5 5 5 80 40 40 11/10/04 C2/C136 B/SINGAPORE/17/2004 5 5 5 5 5 80 40 40 11/19/04 C2/C1
HEMAGGLUTINATION INHIBITION REACTIONS OF INFLUENZA B VIRUSES
Influenza BranchInfluenza Branch
Influenza B Isolates Characterized by CDC
U.S.A. North America
Europe Asia Cent/So America
Africa, Australia,
New Zealand
Total (%)
April 2004 – September 2004 B/Hong Kong/330/2001-like 4 1 2 7 (5%)
%% B/HK/330/2001 (low) 10 1 8 19 (13%) B/Shanghai/361/2002-like 36 6 1 30 24 12 109 (74%) B/Shanghai/361/2002 (low) 4 2 3 3 12 (8%) Total 147 October 2004 – February 2005 B/Hong Kong/330/2001-like 2 2 4 (3%) B/HK/330/2001 (low) 16 9 4 29 (25%) B/Shanghai/361/2002-like 61 3 11 1 76 (64%) B/Shanghai/361/2002 (low) 7 1 1 9 (8%) Total 118
Total 140 6 5 57 40 17 265 Preliminary Data 02/14/2005
Influenza BranchInfluenza Branch
Evolutionary Relationships Among Influenza B/Yamagata HA Genes
0.01
North Carolina/5/2004 LR New Jersey/1/2005 LR
NIMR-Madrid/g1695/2004 Hawaii/18/2004 # LR
Hawaii/19/2004 # LR Egypt/2040/2004
NIMR-Norway/1102/2004 NIID-Yokohama/5/2004
Aus-Victoria/501/2004 # Aus-Brisbane/5/2004 #
California/3/2004 LR Colorado/4/2004 # LR *
Massachusetts/3/2004 Saint Lucia/1689/2004 LR
Washington/2/2004 Peru/1322/2004 LR
Pennsylvania/4/2004 LR Florida/7/2004 # LR * Canada/580/2004
NIID-Hyogo/6/2004 Argentina/584/2004
Concepcion/8881/2004 # Montana/1/2004 # LR
Aus-Christchurch/27/2004 # Aus-Christchurch/33/2004 #
Ulan-Ude/6/2003 # Fujian/437/2004 # LR
Wuhan/281/2004 LR Aus-Victoria/508/2004 #
Jilin/20/2003 # Jiangsu/10/2003 # *
Shandong/22/2003 # Hong Kong/307/2004
Chanthaburi/218/2003 Aus-Brisbane/4/2004 #
NIID-Shizuoka/144/2004 # Shanghai/361/2002 #
Harbin/7/94 # Sichuan/379/99 #
LR - Low reactor to B/Shanghai/361/2002
Vaccine Strain# - egg isolate
* - Reference antigen
V 252 M
N 123 SM 252 I
H 40 YL 131 P
K 129 ND 233 AG 256 R
I 180 V
Influenza BranchInfluenza Branch
Evolutionary Relationships Among Influenza B/Victoria HA Genes
0.01
Hawaii/20/2004 # LR Nevada/1/2005 LR
Washington/1/2004 Hawaii/33/2004 #
Hawaii/32/2004 # Hawaii/31/2004 #
Hawaii/11/2004 # Hawaii/13/2004 # *
Aus-Phitsanulok/2053/2004 * Aus-Surin/1597/2004
Aus-Perth/33/2004 Aus-Perth/12/2004
Kyonggi/501/2004 LR Brazil/1921/2004 LR
New York/9/2004 Saint Lucia/2185/2004 LR
Florida/1/2004 NIID-Sichuan/12/2003 #
Taiwan/1558/2004 Talcahuano/6830/2004 # LR
Wulumuqi/26/2004 # Hong Kong/318/2004 LR
Aus-Malaysia/2276/2004 Hong Kong/312/2004 LR Hong Kong/310/2004 LR
Aus-Malaysia/20/2004 NIMR-Israel/12/2004 Paraguay/636/2003 LR
Aus-Brisbane/32/2002 # Kazakhstan/222/2004 NIID-Akita/9/2003 #
NIMR-Madagascar/72134/2004 Shanghai/47/2003 #
Beijing/243/97 # Shandong/7/97 #
Fujian/203/2003 # NIID-Longyan/11/2003 #
Hong Kong/1434/2002 # Hong Kong/330/2001 #
Taiwan/217/97
LR - Low reactor to B/Hong Kong/330/2001
Vaccine Strain# - egg isolate
* - Reference antigen
K 299 R
A 217 S
K 80 E
K 48 EK 80 RK 129 N
I 121 T
H 116 RI 121 ND 164 E
K 129 E
Influenza BranchInfluenza Branch
Evolutionary Relationships Among Influenza B NA Genes
Sh
d/S
ic Re
asso
rtan
ts
Taiwan/1547/2004 Jiangsu/10/2003#*
Jilin/20/2003 nihj-Shizuoka/2/2004 Shandong/22/2003# Fujian/437/2004# Fujian/393/04#
HongKong/64/2004 Fujian/430/2004(LR)*
Shanghai/361/2002 Shizuoka/58/2004# nihj-shizuoka/58/2004
Sichuan/379/99 aus-Brisbane/32/2002
Madagascar/72134/2004 Paraguyy/6362003 Wulumuqi/26/2004
Talcahuano/6830/2004# Peru/658/2004
Phitsanulok/2053/2004(LR)* Okinawa/1/2004
Hawaii/10/2004 Hawaii/09/2004# Hawaii/20/2004# Hawai/13/2004(LR)#*
UlanUde/6/2003 Brazil/658/2004 Florida/7/2004(LR)#*
Concepcion/8881/2004# Canada/508/2004 Victoria/509/2004#
Christchurch/33/2004# Victoria/50/2004
Oslo/586/2004 Brisbane/05/2004# nimr-Norway/110-2/2004
Massachusetts/3/2004 NewYork/17/2004 Colorado/03/2004# Colorado/05/2004# Colorado/4/2004#*
Harbin/7/94 Yamagata/16/88
Victoria/2/87 Shandong/7/97 HongKong/330/2001
Singapore/222/79
nucleotides
*Serology antigen(LR) Low reaction in HI# egg isolate vaccine
0 Scale 7
K125N, S397ID463N
T42P, E148G,S198NI248V, N342D, K373ET389A
T42Q, T49I, K186RN219K, N235D, S244PD329N, N324D, D392E E436T
Influenza BranchInfluenza Branch
B Summary: October 2004 to February 2005
Influenza B viruses continued to circulate in many countries
The majority of analyzed B viruses belonged to the B/Yamagata/16/88 lineage and were closely related antigenically to the B/Shanghai/361/2002 vaccine strain
Most B/Victoria/2/87 lineage viruses analyzed were reassortants, bearing a B/Hong Kong/330/2001-like HA gene and a B/Sichuan/379/99-like NA gene.
B/Victoria/2/87 lineage viruses were antigenically distinguishable from B/Hong Kong/330/2001, the previous vaccine strain from this lineage
Influenza BranchInfluenza Branch
Influenza Virus Isolates Characterized by CDC and Other WHO Collaborating Centers and Laboratories *
(October 2004 – February 2005)
North
America Europe Asia
Cent/So America
Africa, Oceania
Total (%)
A(H1N1) 2 115 74 2 193 (8%)
A(H3N2) 616 402 212 4 458 1692 (75%)
B/Yamagata 98 62 30 1 112 303 (14%)
B/Victoria 18 28 16 3 9 74 (3%)
Total 734 607 332 8 581 2262 Preliminary Data 02/14/2005
* Data based on cumulative influenza virus isolates antigenic characterization data provided by WHO Collaborating Centers in Atlanta, London, Melbourne, Tokyo, reports from European Influenza Surveillance Scheme and National Influenza Center, Canada