INFLUENCE OF POLYBAG SIZE AND WATERING REGIMES … · Hevea brasiliensis or rubber planting has...

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UNIVERSITI PUTRA MALAYSIA INFLUENCE OF POLYBAG SIZE AND WATERING REGIMES ON GROWTH OF HEVEA BRASILIENSIS MÜLL. ARG. SEEDLINGS IN THREE SOIL SERIES NOORSUHAILA BINTI ABU BAKAR FP 2015 19

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UNIVERSITI PUTRA MALAYSIA

INFLUENCE OF POLYBAG SIZE AND WATERING REGIMES ON GROWTH OF HEVEA BRASILIENSIS MÜLL. ARG. SEEDLINGS IN

THREE SOIL SERIES

NOORSUHAILA BINTI ABU BAKAR

FP 2015 19

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INFLUENCE OF POLYBAG SIZE AND WATERING REGIMES ON

GROWTH OF HEVEA BRASILIENSIS MÜLL. ARG. SEEDLINGS IN THREE

SOIL SERIES

By

NOORSUHAILA BINTI ABU BAKAR

Thesis Submitted to the School of Graduate Studies, Universiti Putra Malaysia, in

Fulfillment of the Requirements for the Degree of Master of Science

May 2015 © COPYRIG

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All material contained within the thesis, including without limitation text, logos, icons, photographs

and all other artwork, is copyright material of Universiti Putra Malaysia unless otherwise stated. Use

may be made of any material contained within the thesis for non-commercial purposes from the

copyright holder. Commercial use of material may only be made with the express, prior, written

permission of Universiti Putra Malaysia.

Copyright © Universiti Putra Malaysia

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Hevea brasiliensis or rubber planting has shifted to the dry marginal land. This can give rise to a higher plant

death or damage during field transplanting. By adopting high quality planting material and high yielding clones

could reduce mortality, immaturity period of Hevea tree and replace dead seedlings during transplanting.

Appropriate polybag size should be determined to be used as planting material for establishment of an advanced

planting material (APM) in nursery. An experiment was conducted to investigate the growth performance and

suitability of two clones, RRIM 2025 and RRIM 3001 in different polybags sizes for commercial cultivation. Reduction in polybag sizes reduced growth performance as reflected by the growth rate of H. brasiliensis at the

nursery stage. A large polybag results in vigorous performance for rubber seedlings and would be ideal for use

as advanced planting material. Clone RRIM 3001 was recommended for planting purpose due to its vigor during

establishment stage.

In experiment 2, the response of latest clone of Hevea brasiliensis towards water stress on different soil series

was investigated. The shoot and stem dry weight of Hevea clone decreased significantly with decrease watering

frequencies. Fine texture encourages root development within soil. Water use efficiency was greater for clone

RRIM 30001 and has ability of plants to conserve water during stress. Water stress strongly affected growth and

physiological characteristics of RRIM 3001 grown but did not affect soil series. Clay and sandy clay soil soils

provide good conditions for crop establishment.

The objective in experiment 3 is to study the effects of different field capacity and soil series on growth and

physiological response of Hevea brasiliensis, RRIM 3001. Plants grown on Munchong Series soil recorded the

highest value for plant growth parameters rather than Serdang and Sungai Buloh Series soil series. Water

requirement for each soil series was differed between each soil series.

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Abstract of thesis presented to the Senate of Universiti Putra Malaysia in fulfillment of the requirements for

the degree of Master of Science

INFLUENCE OF POLYBAG SIZE AND WATERING REGIMES ON GRWTH OF HEVEA

BRASILIENSIS MÜLL. ARG SEEDLINGS IN THREE SOIL SERIES

By

NOORSUHAILA BINTI ABU BAKAR

May 2015

Chairman :Assoc. Prof. Wan Mohamed Noordin Wan Daud, PhD

Faculty : Agriculture

Hevea brasiliensis or rubber planting has shifted to the dry marginal land. This can give rise to a higher

plant death or damage during field transplanting, requiring immediate replacement of dead plants especially

young Hevea seedlings. By adopting high quality planting material and high yielding clones could reduce

mortality, reduce immaturity period of Hevea tree and replace dead seedlings during transplanting.

Appropriate polybag size should be determined to be used as planting material for establishment of an

advanced planting material (APM) in nursery. Thus, an experiment was conducted to investigate the growth

performance and suitability of two clones, RRIM 2025 and RRIM 3001 in different polybags sizes for

commercial cultivation. Small polybag sizes inhibit greatly plant height and girth circumferences for both clones. Meanwhile, large polybags encouraged vigorous root growth as more elongate root was developed

to enhance water and nutrient uptake. Root development was closely associated with the volume of soil

within two polybags size as shown in root:shoot ratio. Root: shoot ratio increased linearly to increase in size

of polybags. Plant shoot growth was inhibited more in small polybags than the roots despite the restricted

rooting space. Root length and root volume were also significantly influenced by polybag size. This

suggests that a reduction in polybag sizes reduced growth performance as reflected by the growth rate of H.

brasiliensis at the nursery stage. It was concluded that a large polybag results in vigorous performance for

rubber seedlings, and that it would be ideal for use in raising young-budded Hevea tree as advanced

planting material before establishment in the field. Comparison between clones, clone RRIM 3001 was

recommended for planting purpose due to its vigor during establishment stage and its capability to produce

more roots which lead to search water and nutrient effectively on dry marginal land. However, study on

watering regimes is needed for new LTC which is lacking in literature review.

In experiment 2, the response of latest clone of Hevea brasiliensis towards water stress on different soil

series was investigated. The shoot and stem dry weight of Hevea clone decreased significantly with

decrease watering frequencies. The highest values for shoot and stem dry weight were found in well-

watered treatment. However, no significant difference was observed between soil series. Root:shoot ratio

was significantly greater under treatment withholding water for eight days especially plants when grown on

Munchong Series compared to Serdang and Sungai Buloh Series soil due to fine texture which encourages

root development within soil. Photosynthesis rate was significantly lower in treatment of watering intervals

every eight days. This accompanied with reduction in stomatal conductance and transpiration rate.

However, water use efficiency was greater under treatment of watering intervals every eight days. This indicates the ability of plants to conserve water during stress. Total leaf area significantly decreased as

watering intervals increased and affected Fv/FM ratio. In conclusion, water stress strongly affected growth

and physiological characteristics of RRIM 3001 grown in different soils in Malaysia. Different soil series

did not affected by water stress. However, in case of planting clone RRIM 3001 for rubber production, both

clay and sandy clay soil like Munchong and Serdang Series soils provide good conditions for crop

establishment and not sandy soil like Sungai Buloh Series soil.

The objective in experiment 3 is to study the effects of different field capacity and soil series on growth and

physiological response of Hevea brasiliensis, RRIM 3001. Plants grown on Munchong Series soil recorded

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the highest value for plant height, plant girth, leaves dry weight, total leaf area and chlorophyll content

followed by Serdang, then Sungai Buloh Series soil series. Overall plant receiving higher level of field

capacity recorded significant higher in plant height, plant girth, leaves dry weight, stem dry weight, total

leaf area, chlorophyll content, photosynthesis rate and stomatal conductance as compared with plants

receiving lower level of field capacity. The root to shoot ratio of clone RRIM 3001 increased when

subjected to decrease level of field capacity. Photosynthesis rate and stomatal conductance was reduced when applying excess water. Thus, water requirement for each soil series was differed and full capacity

level is not enough to sustain plant growth especially in dry marginal land and facing environmental stress.

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Abstrak tesis yang dikemukakan kepada Senat Universiti Putra Malaysia sebagai memenuhi keperluan

ijazah Master Sains

PENGARUH SAIZ POLIBAG DAN REJIM PENGAIRAN PADA PERTUMBUHAN ANAK

POKOK HEVEA BRASILIENSIS MÜELL. ARG. DALAM TIGA SIRI TANAH

Oleh

NOORSUHAILA BINTI ABU BAKAR

Mei 2015

Pengerusi : Assoc. Prof. Wan Mohamed Noordin Wan Daud, PhD

Fakulti : Pertanian

Penanaman getah telah beralih ke tanah marginal yang lebih kering. Hal yang demikian akan menyebabkan ketahanan pokok berkurang dan menyebabkan kematian disebabkan penanaman di

tanah yang marginal semasa penanaman di peringkat ladang dan memerlukan pokok gantian yang

lain. Penyediaan bahan tanaman lanjut (APM) di tapak semaian akan menjadi penyelesaian yang

baik. Berat kering akar untuk kedua-dua klon getah, RRIM 2025 dan RRIM 3001 terjejas disebabkan

oleh polibeg bersaiz lebih kecil. Panjang akar dan jumlah akar juga dipengaruhi oleh saiz polibeg. Ini

menunjukkan bahawa pengurangan dalam saiz polibeg menyebabkan penurunan kepada kadar

pertumbuhan H. brasiliensis pada peringkat tapak semaian. Kesimpulannya, polibeg besar sangat

sesuai untuk pertumbuhan anak pokok getah, dan ia akan menjadi pendekatan yang berguna dalam

meningkatkan anak pokok Hevea sebagai bahan tanaman lanjut sebelum dipindahkan ke ladang. Klon

RRIM 3001 dicadangkan untuk tujuan penanaman dan merujuk kepada kerintangan klon ini semasa

pertumbuhan di nurseri.

Dalam eksperimen 2, respon klon terbaru Hevea brasiliensis terhadap kekurangan air pada siri tanah

yang berbeza telah dijalankan. Berat kering bagi daun dan batang Hevea klon menurun dengan ketara

dengan pengurangan kekerapan siraman. Nilai tertinggi untuk berat kering daun dan batang diperoleh

daripada kekerapan siraman yang dilakukan setiap hari.Walau bagaimanapun, tiada perbezaan antara

siri tanah. Nisbah akar kepada daun didapati lebuh tinggi untuk kekerapan siraman setiap lapan hari di

atas tanah Siri Serdang dan Sungai Buloh. Luas permukaan daun berkurangan pada rawatan kekerapan

siraman selama lapan hari dan ini menjejaskan nisbah FV/FM. Kesimpulannya, kekurangan air

menjejaskan pertumbuhan dan ciri-ciri fisiologi RRIM 3001 yang berada di atas tanah di Malaysia

seperti yang ditunjukkan dalam rawatan siraman setiap 8 hari sekali.

Objektif eksperimen 3 adalah untuk mengkaji kesan perbezaan kapasiti ketepuan air pada siri tanah

yang berbeza kepada pertumbuhan dan tindak balas fisiologi Hevea brasiliensis, RRIM 3001. Pokok

yang ditanam di tanah Siri Munchong mencatatkan nilai tertinggi untuk ketinggian tumbuhan, ukur

lilit pokok, berat kering daun, luas permukann daun dan kandungan klorofil diikuti oleh Siri Serdang,

kemudian tanah Siri Sungai Buloh. Pokok yang menerima kadar kapasiti ketepuan air yang tinggi

mencatatkan pertumbuhan yang tinggi untuk tinggi pokok, ukur lilit pokok, berat kering daun, berat

kering batang, luas permukaan daun, kandungan klorofil, kadar fotosintesis dan stomata konduktans

berbanding dengan pokok yang menerima kapasiti ketepuan air pada tahap yang rendah. Nisbah akar

kepada daun bagi klon RRIM 3001 meningkat apabila tahap ketepuan air yang rendah dan

menunjukkan peralihan asimilasi daripada daun ke akar lebih tinggi berbanding dengan tahap

ketepuan air yang tinggi. Kadar fotosintesis dan stomata konduktans berkurang apabila lebihan air diberikan. Oleh itu, keperluan air bagi setiap siri tanah adalah berbeza dan tahap ketepuan air yang

normal tidak mencukupi untuk mengekalkan pertumbuhan pokok terutama di tanah yang kering dan

tanah yang bermasalah.

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ACKNOWLEDGEMENTS

Bismillahirrahmaanirrahim. First of all, a special appreciation to my supervisory committee member,

Associate Professor Dr. Wan Noordin Wan Daud who always gives me guidance, advice, and comments. I am also sincerely thankful to other committee member, Professor Dr. Che Fauziah Ishak,

who gave me valuable comment and constructive criticisms.

I wish to express my deep appreciation to my parents, Hj Abu Bakar Bin Ahmad and Hjh Zainab Binti

Said for gives me motivation, guidance and also financial support throughout my study. I would like

to express my special appreciation to my husband, Mr Mohd Hadi Akbar bin Basri who always help

and guide me in completing this thesis.

I would like to thank the Malaysian Rubber Board (MRB) for helping in providing seedling and permission to access their library.

Sincere thanks and appreciation are extended to head of laboratory and staff of soil chemistry, soil

physics and physiology laboratory for giving me permission to access to the laboratory and help

during the analysis.

Last but not least, I would like to thank all my friends, Mr Shafar Jefri, Mr Aizul Azfar, Mr

Shawaludin Anis, Mr Faizal Kamaruddin, and Mr Syafiq Hamzah for their help and idea for this

study.

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I certify that a Thesis Examination Committee has met on 29th May 2015 to conduct the final

examination of Noorsuhaila Binti Abu Bakar on her thesis entitled "Influence of Polybag Size and

Watering Regimes on Growth of Hevea brasiliensis Müll. Arg. Seedlings in Three Soil Series" in

accordance with the Universities and University Colleges Act 1971 and the Constitution of the

Universiti Putra Malaysia [P.U.(A) 106] 15 March 1998. The Committee recommends that the student

be awarded the Doctor of Philosophy.

Members of the Thesis Examination Committee were as follows:

Mohd Ridzwan A. Halim, PhD

Associate Professor

Faculty of Agriculture

Universiti Putra Malaysia

(Chairman)

Mohd Razi Ismail, PhD

Professor Institute of Tropical Agriculture

Universiti Putra Malaysia

(Internal Examiner)

Wan Rashidah A. Kadir, PhD

Senior Lecturer

Forest Research Institute Malaysia (FRIM)

Malaysia

(External Examiner)

ZULKARNAIN ZAINAL, PhD

Professor and Deputy Dean

School of Graduate Studies

Universiti Putra Malaysia

Date: 12 August 2015

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This thesis was submitted to the Senate of Universiti Putra Malaysia and has been accepted as

fulfillment of the requirements for the degree of Master of Science. The members of the Supervisory

Committee were as follows:

Wan Mohamed Noordin Wan Daud, PhD

Associate Professor Faculty of Agriculture

Universiti Putra Malaysia

(Chairman)

Che Fauziah Ishak, PhD Professor

Faculty of Agriculture

Universiti Putra Malaysia

(Member)

__________________________

BUJANG BIN KIM HUAT, PhD Professor and Dean School of Graduate Studies

Universiti Putra Malaysia

Date:

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Declaration by graduate student

I hereby confirm that:

this thesis is my original work;

quotations, illustrations and citations have been duly referenced;

this thesis has not been submitted previously or concurrently for any other degree at any other

institutions.

intellectual property from the thesis and copyright of thesis are fully-owned by Universiti Putra

Malaysia, as according to the Universiti Putra Malaysia (Research) Rules 2012;

written permission must be obtained from supervisor and the office of Deputy Vice-Chancellor

(Research and Innovation) before thesis is published (in the form of written, printed or in

electronic form) including books, journals, modules, proceedings, popular writings, seminar

papers, manuscripts, posters, reports, lecture notes, learning modules or any other materials as

stated in the Universiti Putra Malaysia (Research) Rules 2012;

there are no plagiarism or data falsification/ fabrication in the thesis, and scholarly integrity is

upheld as according to the Universiti Putra Malaysia (Graduate Studies) Rules 2003 (Revision

2012-2013) and the Universiti Putra Malaysia (Research) Rules 2012. The thesis has undergone

plagiarism detection software.

Signature: ____________________ Date: _________________

Name and Matric No.: Noorsuhaila Binti Abu Bakar (GS29496)

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Declaration by Member of Supervisory Committee

This is confirm that:

the research conducted and the writing of this thesis was under our supervision;

supervision responsibilities as stated in the to the Universiti Putra Malaysia (Graduate Studies)

Rules 2003 (Revision 2012-2013) are adhered to.

Signature: ____________________

Name of Chairman of Supervisory Committee: Wan Mohamed Noordin Bin Wan Daud, PhD

Signature: ____________________

Name of Member of Supervisory Committee: Che Fauziah Binti Ishak, PhD

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TABLE OF CONTENTS

Page

ABSTRACT i

ABSTRAK iii

ACKNOWLEDGEMENTS iv

APPROVAL v

DECLARATION viii

LIST OF TABLES xii

LIST OF FIGURES xiii

LIST OF ABBREVIATIONS xiv

CHAPTER

1 INTRODUCTION 1

1.1 Objectives

2

2 LITERATURE REVIEW 3

2.1 Morphology of rubber tree, Hevea brasiliensis

2.1.1 Physiology of H. brasiliensis

2.2 Latex Timber Clones

2.3 Nursey

2.3.1 Polybag nursey

2.3.1.1 Effects of polybag size on soil properties

2.3.1.2 Effects of polybag size on plant growth

2.4 Manuring of rubber 2.5 Texture classification

2.5.1 Soil texture

2.5.2 Soil series

2.5.2.1 Munchong Soil Series

2.5.2.2 Serdang Soil Series

2.5.2.3 Sungai Buloh Soil Series

2.6 Soil limitation

2.7 Effect of soil texture on plant growth

2.8 Soil, water and plant relationship

2.9 Soil moisture properties

2.9.1 Field capacity

2.9.2 Permanent wilting point 2.9.3 Available water

2.10 Water deficit

2.10.1 Effect of water deficit on growth response of H. brasiliensis

2.10.2 Effect of water deficit on physiological response of H.

brasiliensis

2.11 Excess water

2.11.1 Effect of excess water on growth response of H. brasiliensis

2.11.2 Effect of excess water on physiological response of H.

brasiliensis

2.11.3 Effect of excess water on soil properties

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3 MATERIALS AND METHODS

3.1 Experimental site

3.2 Planting materials

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3.2.1 Seedlings

3.2.2 Polybag

3.2.3 Planting medium

3.3 Watering

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3.3.1 Water retention method

3.3.2 Water requirement determination

3.4 Fertilization

3.5 Control of pest and disease

3.6 Data collection

3.6.1 Growth analysis 3.6.1.1 Plant height

3.6.1.2 Plant girth

3.6.1.3 Dry weight

3.6.1.4 Root to shoot ratio

3.6.1.5 Root length and root volume

3.6.1.6 Leaf area

3.6.1.7 Chlorophyll content

3.6.2 Physiology analysis

3.6.2.1 Leaf gas exchange

3.6.2.2 Maximum quantum efficiency of photosystem II

(Fv/FM ratio) 3.7 Plant nutrient analysis

3.8 Soil analysis

3.9 Data analysis

18

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20

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20 20

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4 GROWTH PERFORMANCE AND LEAF, STEM AND ROOT

CHARACTERISTICS OF YOUNG IMMATURE RUBBER

(HEVEA BRASILIENSIS) CLONES IN RELATION TO SIZE OF

POLYBAG IN THE NURSERY

24

4.1 Introduction

4.2 Materials and Methods

4.2.1 Experimental site and plant materials 4.2.2 Treatments and experimental design

4.2.3 Measurement of growth performance of Hevea brasiliensis

clones

4.2.4 Measurement of leaf chlorophyll content of Hevea brasiliensis

clones

4.2.5 Sample leaf analysis for nutrient content

4.2.6 Statistical analysis

4.3 Results

4.4 Discussion

4.5 Conclusion

24

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25 25

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5 INFLUENCE OF WATER STRESS ON THE GROWTH AND

PHYSIOLOGICAL PERFORMANCE OF HEVEA

BRASILIENSIS, RRIM 3001 GROWN ON THREE SOIL SERIES

5.1 Introduction

5.2 Materials and methods

35

35

36

5.2.1 Experimental site

5.2.2 Planting materials and experimental design

5.2.3 Soil analysis

5.2.4 Water requirement determination

5.2.5 Plant data collection

5.2.6 Statistical data analysis

5.3 Results 5.3.1 Soil analysis

5.3.2 Water requirement determination

5.3.3 Effect of water stress on growth responses of H. brasiliensis

5.3.4 Effect of water stress on physiological response of H.

brasiliensis

5.4 Discussion

5.5 Conclusion

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6

RESPONSE OF HEVEA BRASILIENSIS CLONE, RRIM 3001 IN

RELATION TO MOISTURE AT DIFFERENT FIELD

CAPACITY IN THREE SOIL SERIES 6.1 Introduction

6.2 Materials and methods 6.3 Results

6.4 Discussion

6.5 Conclusion

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58 59

65

66

7 GENERAL CONCLUSIONS AND RECOMMENDATIONS 67

6.1 General Conclusions

6.2 Recommendation for future research

67

68

REFERENCES

APPENDICES

BIODATA OF STUDENT

LIST OF PUBLICATIONS

69

86

88 89

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LIST OF TABLES

Table

Page

1 Soil series limitation

9

2

Parental crossing of two clones 17

3 Analysis of variance for growth response of latex timber clones 28

4 Effect of polybag size on plant growth of clones RRIM 3001 and RRIM

2025

28

5 Analysis of variance for root length, root volume and chlorophyll content

30

6 Effect of polybag size on root length, root volume and chlorophyll content

of clones RRIM 3001 and RRIM 2025

32

7 Leaf nutrient contents of Hevea clones in polybag of different sizes

33

8 Textural class of three soil series used

38

9 Selected chemical characteristics of three soil series

38

10 Analysis of variance for height and girth increment 40

11 Analysis of variance for growth performance of clone RRIM 3001

43

12 Effect of soil series and watering intervals on height, girth, dry weight, root

to shoot ratio and total leaf area of clone RRIM 3001

44

13 Analysis of variance for photosynthesis rate during experiment period 49

14 Analysis of variance for stomatal conductance, transpiration rate and water

use efficiency during experiment period

50

15 The effect of water treatment on transpiration rate and water use efficiency

53

16 Analysis of variance for chlorophyll content and chlorophyll fluorescence of

clone RRIM 3001

54

17 Selected soil chemical properties of three soil series used after the

experiment

60

18 ANOVA of main effects different water treatment (W), soil series (S) and

their interaction (W×S) for height, girth, leaf dry weight, stem dry weight,

root dry weight, root length, total leaf area, root to shoot ratio, chlorophyll content, photosynthesis rate and stomatal conductance

61

19 The effect of water treatment on height, girth, leaf dry weight, stem dry

weight, root dry weight, root length, total leaf area and root to shoot ratio

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LIST OF FIGURES

Figure

Page

1 USDA Soil Texture Triangle

7

2 Relationship water holding capacity and soil texture 8

3 Water requirement determination for three soil series

19

4 Plant height increment of clone RRIM 3001 and clone RRIM 2025

over time in polybags of different sizes

29

5 Linear relationship between root elongation and time in clone RRIM

3001 and RRIM 2025 grown in polybags of different sizes

31

6 Magnesium deficiency symtoms observed in both polybag sizes

32

7 Clones RRIM 2025 and RRIM 3001 in different size of polybag

33

8 Height increment over time for each soil series

41

9 Girth increment over time for each soil series

42

10 Effect of water stress on root dry weight for three soil series

45

11 Effect of water stress on root length for three soil series

45

12 Effect of water stress on root volume for three soil series

46

13 Root: shoot ratio under water stress treatment

46

14 Photosynthesis rate over month for three soil series

48

15 Stomatal conductance over month for three soil series

51

16 Effect of water stress on chlorophyll content for three soil series

54

17 Effect of water stress on chlorophyll fluorescence for three soil series

55

18 Height of Hevea brasiliensis clone, RRIM 3001 after imposition of

water treatment on different soil series

63

19 Girth of Hevea brasiliensis clone, RRIM 3001 after imposition of

water treatment on different soil series

63

20 Root length of Hevea brasiliensis clone, RRIM 3001 after

imposition of water treatment on different soil series

64

21 Chlorophyll content of Heveabrasiliensis clone, RRIM 3001

after imposition of water stress treatment on different soil

series

65

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LIST OF ABBREVIATIONS

AAS Atomic Absorption Spectrophotometer

ANOVA Analysis of Variance

CEC Cation Exchange Capacity

cmol/kg Centimol per kilogram

Meq

MRB

Miliequivalent

Malaysian Rubber Board

ppm

LSD

RISDA

parts per million

Least Significant Difference

Rubber Industry Smallholders Development Authority

SAS

Sg Buloh

Statistical Analysis System

Sungai Buloh

USDA United States Department of Agriculture

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CHAPTER

INTRODUCTION

Rubber-tree areas are being replaced by more oil palm areas to meet the increasing

demand for food production. The tightness of raw material supply, latex or natural

rubber and shrinkage area for further expansion forced the search for alternate

areas in non-traditional regions of Malaysia. Rubber cultivation is being promoted

in non-traditional areas to meet the demand of rubber production. An unfavourable

growing condition in non-tropical countries prevents the expansion of Hevea

brasiliensis which limit its cultivation to specific environments. The optimal

growing conditions of rubber tree are the following: to mm rainfall

without a dry season; a mean annual temperature of ± ˚C; and sunshine hours

of ~ h year-

, distributed fairly equally in all months. The production and

quality of latex affected when d dry periods more two to three months. However,

rainfall distribution becomes challenge on marginal land due to having stressful

conditions which high temperature or near to drought conditions.

Immature period of rubber tree at plantation is one of the major concerns to most

rubber planter. By adopting high quality planting material and high yielding new

clones are used to reduce unproductive immaturity period since during

establishment stage at nursery. Thus, various aspects of efficient resource

management of a nursery stage needs to be considered to obtain the maximum

economic benefits besides vigorous seedling such as soil fertility, water

requirement and pest and disease management. Therefore, nursery management is

vital to obtain vigour and healthy rubber seedling.

In the culture of rubber, the raising of the small rubber tree using polybags in

nursery stage or polybag nursery is a recommended practise to minimize problems

of transplanting shock after transplanting into the field. Besides that, it can be used

as an approach for preparing advance planting material (APM) to replace dead

seedling during establishment at the field. However, volume of soil available to

the root system has been responsible for reliance on growth performance of the

plant. Therefore, it is important to determine the appropriate polybag size which

occupy soil volume as media to obtain uniform vigour Hevea seedlings and more

resistant to drought and wind at nursery stage. The vigour seedlings reduce

seedlings mortality during transplanting into the field.

Another factor to be considered is soil moisture deficit since it hinders the growth

and crop productivity. Deficient of soil moisture in soil can contribute to decline

of tree growth, growth performance and yield. Numerous studies have been

conducted whereby water deficit reduces growth and yield in many stage of

growth and development of plants. Youngg rubber plants suffer from moisture

stress during dry periods which is a common characteristic in marginal dry area

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and cause drastic plant death. Therefore, it is important to understand the effect of

soil moisture stress on the performance of Hevea seedling at nursery stage.

Another environmental stress is excessive soil moisture or waterlogging which

could results in reduction of plant growth rate, wilting, and eventual death of plant

due to depletion of soil oxygen. Most of Hevea seedling cannot withstand under

excessive water stress like flooding conditions which cause root dieback then

plant death. Therefore, it is important to investigate the effects of excessive water

on growth and physiology of Hevea clone at nursery stage which pertinent to soil

texture. Specific water requirement at nursery stage is required to obtain vigour

Hevea seedlings before transplanting into the field. Therefore, a research on the

water relation and requirement is needed when rubber cultivation was extended to

dry regions with emphasis on agronomy aspect.

Malaysian Rubber Board has recommended the latest high-yielding rubber clone

which is clone RRIM or ‘Clone Malaysia’ for used for traditional region or

rubber zone area which has proven to increase yield. However information regard

to latest clones planted in non-traditional areas is lacking. Besides that, a few

abiotic constraints are unfavourable to rubber cultivation especially on Malaysia

soils such as acid sulphate soil and poorly drained soil. Therefore, assessing the

suitability of soils under rubber is required for agronomic recommendation and

future expansion planning. Thus, the information on the suitability and

adaptability of the latest Hevea clones is required to adapt to new environments.

The study of environment stress like drought and waterlogging are needed to

interpret adaptation of latest clone in saturated and dry soil moisture regimes.

Objectives

The main objectives of this study are to evaluate new latex timber clone of Hevea

brasiliensis, for nursery establishment and plantation. The specific objectives are:

. To investigate the growth performance and suitability of two Hevea

clones, RRIM and RRIM in different polybag sizes for

commercial cultivation.

. To evaluate the effect of water stress on growth performance and

physiological response of new latex timber clone, RRIM grown in

different soil series.

. To examine growth performance and physiological response of new latex

timber clone, RRIM at different levels field capacity as influenced

by these soil series.

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