INDOOR CHAETOMIUM-LIKE ISOLATES: RESISTANCE TO … · Emmanuelle Castagnoli 4 *Mikkola et al....
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INDOOR CHAETOMIUM-LIKE ISOLATES: RESISTANCE TO CHEMICALS, FLUORESCENCE AND MYCOTOXIN PRODUCTION Emmanuelle Castagnoli Sisäilmastoseminaari 15.03.2017
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Background
• Chaetomium species
• Most common representative of Chaetomiaceae in indoor environments
• Produce >500 bioactive substances
• E.g. Chaetomium globosum
• Known human pathogen
• Linked to asthma in mould-infested buildings
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Chaetoviridin A
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Scope of the study
To investigate Chaetomium-like indoor isolates in Finland
• species diversity
• biocide/chemical resistance
• toxicity towards mammalian cells
• metabolite identification
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e.g. mammalian lung cell.
alive vs. dead
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Isolation
1. Sampling in urban and rural buildings where severe health
problems were observed among humans and piglets
2. Chaetomium-like isolates
1. Examination of ascomata**
2. Revealed toxic in a rapid toxicity screening*
3. Pure cultured on MEA
4. Ethanol-soluble compounds from biomasses were collected
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*Mikkola et al. (2015) Toxic indole alkaloids avrainvillamide and stephacidin B
produced by a biocide tolerant indoor mold Aspergillus westerdijkiae, Toxicon
**Wang et al. (2016) Diversity and taxonomy of Chaetomium and chaetomium-
like fungi from indoor environments
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Identification of the fungal strains
• Colony morphology
• Conidiophore morphology
• Size of conidia
• Fluorescence
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MO15
(chaetomium)
MTAV 35 (Ch.
Globosum)
chaetoglobosin
producer
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Characterisation of Chaetomium-like isolates
Isolate Isolated from Substratum Fluorescence Size of
ascospores
(μm)
MTAV 35 Public building,
Oulu
Settled dust green 10.6 × 9*
MTAV 37 Public building,
Oulu
Settled dust green 10.5 × 9*
MH1 Public building,
Espoo
Settled dust green 8.5 × 7.6*
RUK 10 Apartment, Helsinki Settled dust green 10.4 × 8.8*
ABCD Apartment, Helsinki Settled dust green 9 × 7.6*
MO9 Piggery, Orimattila Bedding green 10.3 × 7.6*
2c/MT Apartment, Vantaa Settled dust green 9.5 × 7.3*
MO15 Piggery, Orimattila Bedding yellow 12 × 8**
Ch1/tu Public building,
Espoo
Inlet air filter blue 5.7 × 4.1**
OT7 Office, Helsinki Settled dust none 8.9 × 7.8*
OT7b Office, Helsinki Settled dust none 9 × 7.7*
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Biocide resistance
• Tested: Borax, Boracol, PHMB, Fenoxy-ethanol,
genapol chloramine and triclosan
• inhibition of fungal spore germination
• inspected by phase contrast microscopy after 1 and 2 d of
incubation at 28ºC
• The tests:
• Run in triplicate
• Calibrated with triclosan (mean SD ±< 20 %)
• EC50 = inhibition of 50% of the conidia compared to the ethanol
control (1 %).
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germ tube
(bar 10µm)
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EC50 (µg.ml-1)
Borax Boracol PHMB Genapol Fenoxy-ethanol
Chloramine Triclosan
Indoor Chaetomium-like strains
Green >5000 100 4-8 <50 700-1500 1200-2500 2-4
Blue 1200 100 4 <50 1500 1200 2
Yellow 5000 100 4 50000 1500 1200 2
Mammalian cell lines (MSCT assay)
MNA 150 <50 4 25 400 80 4
PK-15 600 <50 15 25 1500 150 15
Reference strains
Indoor Trichoderma atroviride isolates d
1200 100 4 >50000 1500-3000 1200 16-30
Outdoor Trichoderma atroviride isolates e 1200 100 4-16 >50000 1500-3000 1200-2500 8-16
Indoor Aspergillus isolates f >5000 400-800 30-60 >50000 1500-3000 600-1200 8-16
Indoor Aspergillus isolates g 5000 100 30-60 >50000 800-400 600-1200 16
Indoor Paecilomyces isolates h >5000 400 30 >5000 3000 1200 4
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EC50 (µg.ml-1)
Borax Boracol PHMB Genapol Fenoxy-ethanol
Chloramine Triclosan
Indoor Chaetomium-like strains
Green >5000 100 4-8 <50 700-1500 1200-2500 2-4
Blue 1200 100 4 <50 1500 1200 2
Yellow 5000 100 4 50000 1500 1200 2
Mammalian cell lines (MSCT assay)
MNA 150 <50 4 25 400 80 4
PK-15 600 <50 15 25 1500 150 15
Reference strains
Indoor Trichoderma atroviride isolates d
1200 100 4 >50000 1500-3000 1200 16-30
Outdoor Trichoderma atroviride isolates e 1200 100 4-16 >50000 1500-3000 1200-2500 8-16
Indoor Aspergillus isolates f >5000 400-800 30-60 >50000 1500-3000 600-1200 8-16
Indoor Aspergillus isolates g 5000 100 30-60 >50000 800-400 600-1200 16
Indoor Paecilomyces isolates h >5000 400 30 >5000 3000 1200 4
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The fungal isolates were more resistant than the mammalian cell lines PK-15 and MNA
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EC50 (µg.ml-1)
Borax Boracol PHMB Genapol Fenoxy-ethanol
Chloramine Triclosan
Indoor Chaetomium-like strains
Green >5000 100 4-8 <50 700-1500 1200-2500 2-4
Blue 1200 100 4 <50 1500 1200 2
Yellow 5000 100 4 50000 1500 1200 2
Mammalian cell lines (MSCT assay)
MNA 150 <50 4 25 400 80 4
PK-15 600 <50 15 25 1500 150 15
Reference strains
Indoor Trichoderma atroviride isolates d
1200 100 4 >50000 1500-3000 1200 16-30
Outdoor Trichoderma atroviride isolates e 1200 100 4-16 >50000 1500-3000 1200-2500 8-16
Indoor Aspergillus isolates f >5000 400-800 30-60 >50000 1500-3000 600-1200 8-16
Indoor Aspergillus isolates g 5000 100 30-60 >50000 800-400 600-1200 16
Indoor Paecilomyces isolates h >5000 400 30 >5000 3000 1200 4
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• The green-fluorescent isolates: • 4-fold more resistant to borax than the Trichoderma atroviride • quite sensitive to the other biocides compared to the other
reference strains
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EC50 (µg.ml-1)
Borax Boracol PHMB Genapol Fenoxy-ethanol
Chloramine Triclosan
Indoor Chaetomium-like strains
Green >5000 100 4-8 <50 700-1500 1200-2500 2-4
Blue 1200 100 4 <50 1500 1200 2
Yellow 5000 100 4 50000 1500 1200 2
Mammalian cell lines (MSCT assay)
MNA 150 <50 4 25 400 80 4
PK-15 600 <50 15 25 1500 150 15
Reference strains
Indoor Trichoderma atroviride isolates d
1200 100 4 >50000 1500-3000 1200 16-30
Outdoor Trichoderma atroviride isolates e 1200 100 4-16 >50000 1500-3000 1200-2500 8-16
Indoor Aspergillus isolates f >5000 400-800 30-60 >50000 1500-3000 600-1200 8-16
Indoor Aspergillus isolates g 5000 100 30-60 >50000 800-400 600-1200 16
Indoor Paecilomyces isolates h >5000 400 30 >5000 3000 1200 4
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Green- and blue-fluorescent isolates were > 1 000 times more sensitive to genapol than the yellow-fluorescent isolate and the reference strains
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EC50 (µg.ml-1)
Borax Boracol PHMB Genapol Fenoxy-ethanol
Chloramine Triclosan
Indoor Chaetomium-like strains
Green >5000 100 4-8 <50 700-1500 1200-2500 2-4
Blue 1200 100 4 <50 1500 1200 2
Yellow 5000 100 4 50000 1500 1200 2
Mammalian cell lines (MSCT assay)
MNA 150 <50 4 25 400 80 4
PK-15 600 <50 15 25 1500 150 15
Reference strains
Indoor Trichoderma atroviride isolates d
1200 100 4 >50000 1500-3000 1200 16-30
Outdoor Trichoderma atroviride isolates e 1200 100 4-16 >50000 1500-3000 1200-2500 8-16
Indoor Aspergillus isolates f >5000 400-800 30-60 >50000 1500-3000 600-1200 8-16
Indoor Aspergillus isolates g 5000 100 30-60 >50000 800-400 600-1200 16
Indoor Paecilomyces isolates h >5000 400 30 >5000 3000 1200 4
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The blue-fluorescent isolate was sensitive to borax
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EC50 (µg.ml-1)
Borax Boracol PHMB Genapol Fenoxy-ethanol
Chloramine Triclosan
Indoor Chaetomium-like strains
Green >5000 100 4-8 <50 700-1500 1200-2500 2-4
Blue 1200 100 4 <50 1500 1200 2
Yellow 5000 100 4 50000 1500 1200 2
Mammalian cell lines (MSCT assay)
MNA 150 <50 4 25 400 80 4
PK-15 600 <50 15 25 1500 150 15
Reference strains
Indoor Trichoderma atroviride isolates d
1200 100 4 >50000 1500-3000 1200 16-30
Outdoor Trichoderma atroviride isolates e 1200 100 4-16 >50000 1500-3000 1200-2500 8-16
Indoor Aspergillus isolates f >5000 400-800 30-60 >50000 1500-3000 600-1200 8-16
Indoor Aspergillus isolates g 5000 100 30-60 >50000 800-400 600-1200 16
Indoor Paecilomyces isolates h >5000 400 30 >5000 3000 1200 4
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The yellow-fluorescent isolate was resistant to both genapol and borax, similarly to the Aspergillus and Paecilomyces strains
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Metabolite and toxicity profiling
• Ethanol-soluble compounds from biomasses
• boar sperm motility inhibition (BMSI)
• sperm membrane integrity disruption (SMID)
• mammalian somatic cell toxicity (MSCT)
• Continuous cell line PK-15 (porcine kidney cells)
• malignant cell line MNA (murine neuroblastoma cell line)
• as described by Bencsik et al. /3/, except that in BMSI the exposure was
20 min at 37°C.
• Mycotoxins identified with LC/MS
• Pure mycotoxins were purchased from Sigma-Aldrich Finland.
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Strain
EC50 (µg.ml-1) Identified metabolite
Estimated concentration mg/ml a BMSI
20 min
SMID 2h
MSCT 2d
Ch. globosum MTAV35 5 450 40 Chaetoglobosins 3.4
Chaetoviridin A 0.02
Chaetoviridin C 0.2
Ch. globosum MTAV37 10 350 30 No data MH1 5 310 50 Chaetoglobosins 3.9
Chaetoviridin A 0.5
Chaetoviridin C 0.2
RUK 10 5 300 20 Chaetoglobosins 4.2
Chaetoviridin A 0.04
Chaetoviridin C 0.05
ABCD 5 450 30 Chaetoglobosins 4.24
Chaetoviridin A 0.3
Chaetoviridin C 0.05
2c/MT Chaetoglobosinsb OT7 10 480 0.5 Chaetomin 1.3
Chaetoviridins A 0.13
Chaetoviridin C 0.02
OT7b 10 480 0.8 Chaetomin 1.2
Chaetoviridin A 0.3
Chaetoviridin C 0.2
Commercial pure mycotoxins * Biological activity
Alamethicin 5 1 8 K+ and Na + ion channel former Chaetoglobosin A 1 12 2 Inhibitor of glucose transport Citrinin >100 50 10 Cytotoxic, nephrotoxic
Sterigmatocystin >20 >20 0.5 Inhibitor of protein synthesis
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Strain
EC50 (µg.ml-1) Identified metabolite
Estimated concentration mg/ml a BMSI
20 min
SMID 2h
MSCT 2d
Ch. globosum MTAV35 5 450 40 Chaetoglobosins 3.4
Chaetoviridin A 0.02
Chaetoviridin C 0.2
Ch. globosum MTAV37 10 350 30 No data MH1 5 310 50 Chaetoglobosins 3.9
Chaetoviridin A 0.5
Chaetoviridin C 0.2
RUK 10 5 300 20 Chaetoglobosins 4.2
Chaetoviridin A 0.04
Chaetoviridin C 0.05
ABCD 5 450 30 Chaetoglobosins 4.24
Chaetoviridin A 0.3
Chaetoviridin C 0.05
2c/MT Chaetoglobosinsb OT7 10 480 0.5 Chaetomin 1.3
Chaetoviridins A 0.13
Chaetoviridin C 0.02
OT7b 10 480 0.8 Chaetomin 1.2
Chaetoviridin A 0.3
Chaetoviridin C 0.2
Commercial pure mycotoxins * Biological activity
Alamethicin 5 1 8 K+ and Na + ion channel former Chaetoglobosin A 1 12 2 Inhibitor of glucose transport Citrinin >100 50 10 Cytotoxic, nephrotoxic
Sterigmatocystin >20 >20 0.5 Inhibitor of protein synthesis
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Green-fluorescent: more toxic in the sperm assay than in the MSCT
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Strain
EC50 (µg.ml-1) Identified metabolite
Estimated concentration mg/ml a BMSI
20 min
SMID 2h
MSCT 2d
Ch. globosum MTAV35 5 450 40 Chaetoglobosins 3.4
Chaetoviridin A 0.02
Chaetoviridin C 0.2
Ch. globosum MTAV37 10 350 30 No data MH1 5 310 50 Chaetoglobosins 3.9
Chaetoviridin A 0.5
Chaetoviridin C 0.2
RUK 10 5 300 20 Chaetoglobosins 4.2
Chaetoviridin A 0.04
Chaetoviridin C 0.05
ABCD 5 450 30 Chaetoglobosins 4.24
Chaetoviridin A 0.3
Chaetoviridin C 0.05
2c/MT Chaetoglobosinsb OT7 10 480 0.5 Chaetomin 1.3
Chaetoviridins A 0.13
Chaetoviridin C 0.02
OT7b 10 480 0.8 Chaetomin 1.2
Chaetoviridin A 0.3
Chaetoviridin C 0.2
Commercial pure mycotoxins * Biological activity
Alamethicin 5 1 8 K+ and Na + ion channel former Chaetoglobosin A 1 12 2 Inhibitor of glucose transport Citrinin >100 50 10 Cytotoxic, nephrotoxic
Sterigmatocystin >20 >20 0.5 Inhibitor of protein synthesis
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Pure Chaetoglobosin A + chaetoglobosin-containing extracts had BSMI [EC50] < 10 * SMID [EC50] Ccl: immobilization of sperm cells but no plasma membrane disruption (e.g. alamethicin)
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Strain
EC50 (µg.ml-1) Identified metabolite
Estimated concentration mg/ml a BMSI
20 min
SMID 2h
MSCT 2d
Ch. globosum MTAV35 5 450 40 Chaetoglobosins 3.4
Chaetoviridin A 0.02
Chaetoviridin C 0.2
Ch. globosum MTAV37 10 350 30 No data MH1 5 310 50 Chaetoglobosins 3.9
Chaetoviridin A 0.5
Chaetoviridin C 0.2
RUK 10 5 300 20 Chaetoglobosins 4.2
Chaetoviridin A 0.04
Chaetoviridin C 0.05
ABCD 5 450 30 Chaetoglobosins 4.24
Chaetoviridin A 0.3
Chaetoviridin C 0.05
2c/MT Chaetoglobosinsb OT7 10 480 0.5 Chaetomin 1.3
Chaetoviridins A 0.13
Chaetoviridin C 0.02
OT7b 10 480 0.8 Chaetomin 1.2
Chaetoviridin A 0.3
Chaetoviridin C 0.2
Commercial pure mycotoxins * Biological activity
Alamethicin 5 1 8 K+ and Na + ion channel former Chaetoglobosin A 1 12 2 Inhibitor of glucose transport Citrinin >100 50 10 Cytotoxic, nephrotoxic
Sterigmatocystin >20 >20 0.5 Inhibitor of protein synthesis
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Non-fluorescent: • 100-fold more toxic in
MSCT than the chaetoglobosin-producing (green- fluorescent) same behavior as sterigmatocystin
• The inhibitor of protein synthesis chaetomin was present
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Conclusion
• Fluorescence, biocide/genapol resistance, as well as toxicity- and
metabolite profiling may be useful in preliminary tracking of diversity
in the case of indoor ascomata-producing Chaetomium-like isolates.
• The results demonstrate species variability in biocide/chemical
resistance among indoor fungal species and genera. The indoor use
of biocides and chemicals may influence proliferation and species
diversity of the indoor microbiota.
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Merci!
Department of Civil Engineering, Aalto University
Financial support from
TEKES (grant 4098/31/2015)
the Academy of Finland (TOXICPM 289161)
Project GINOP-2.2.3-15-2016-00012
The somatic cells from Riika Holopainen (Finnish Food
Safety Authority EVIRA/Virology)