By

Fareeha Shireen

Conservation of Exotic Lavender

EXOTIC PLANTS

A species occurring in a given place outside of its native range which has arrived there by human activity, either deliberate or accidental.

Cycad (Encephalartos woodii) Sweet clover Passiflora Lavender lady

MERITS AND DEMERITS OF EXOTIC PLANTS

Merits• Introduction sets an ideal stage

for evolutionary diversification among populations of exotic species

Demerits• Invasion of Exotic Species• A species outside of its native

range that threatens the survival or reproduction of native plants is known as invasive specie

CONSERVATION

The action of Conserving something, particularly preservation or restoration of natural environment and of wild life

In vitro conservation of plant germplasm is a technique used specifically for those plants which do not produce seed

In vitro Conservation is ideal method for conservation of plant germplasm because it require minimum space, Labour and cost

MEDICINAL PLANTS

Tansy Mexican butterfly Lady-fern Marijuana

California-poppyBlack-berriesRed-clover Wild quinine

2,50,000 Higher Plant Species on Earth > 80,000 are medicinal plants

Lavender (Lavendula-spp)

Latin Word “Lavere” that’s mean “To wash” or “To Bath”

Belongs to Family (Lamiaceae) Originated from North Africa, Europe

and India 39 species, numerous hybrids and

400 registered cultivars having aromatic and medicinal properties.

Lavender oil is used to treat acne Anxioxidant and Antiabcterial propertesoil is used in AromatherapySoothing effectRelieve in depression and tensionOrnamental

History

Origin

Significance

MORPHOLOGY CHARACTERS

Gray foliage & purple

flowers

Leaves are eight times as

Longer as Wider

leaves are 5cm long

Plants are 2 to 3 feet tall

but some cultivars are

shorter

Leaf shape is oblong.

Plant tissue culture can defined as growing and multiplication of cells, tissues and organs of plants on defined solid or liquid media under aseptic environment and controlled conditions of light, temperature and humidity.

TISSUE CULTURE

OBJECTIVES OF STUDY

To study the best sterilization method for in vitro propagation of lavender.

To study the effect of different concentration of hormones on the micropropagation of lavender

Multiplication of Lavender by vegetative means ( in vitro multiplication).

To evaluate the antioxidant activity of lavender.

MATERIAL AND

METHOD

MICRO PROPAGATION

Explant selection Explant preparation and its

disinfection Media preparation Explant culture and incubation Growth regulators in in vitro

culture

EXPLANT SELECTION

Shoot cutting from mature and Healthy

plant grown in field

Apical and lateral part was selected For in

vitro propagation

Cont…..

• Washing with Tween 20• Surface sterilized with 5%, 7%,

10%, 12% & 15% sodium hypochlorite

Surface Sterilization

• MS Media with Stocks, Myo, vitamin, Sugar and Agar

• PH : 5.8• Autoclave media at 121ºC for 7 minutes

Media Preparation

MS MEDIA COMPOSITIONSr. No Stocks 1000ml Functions

1 A 10ml Increase resistance to outside attack and play role in producing plant tissue

2 B 50ml Form cellulose, lignin and important role in reservation of food

3 C 5ml Improves growth of roots and callus4 D 5ml Promote root formation and maturity5 E 5ml Helps to develop enzyme and vitamin, and

chlorophyll6 F 5ml Stimulate growth and increase leaf Area7 Myo 5ml Influence growth and development8 Vitamin 1ml Essential compound for growth and plant

metabolism9 Sucrose 30g Provide neutrients

10 Agar 7g Aid in solidification of media11 The p H of media should be 5.8

Cont….

Explant Culture And incubation

• Cuttings are further asetically cut

• Implant vertically in test tubes

Growth Regulators

• Three hormonal medias were used, M1 MS media, M2 BAP(0.1, 0.2, 0.3)+NAA(0.1) and M3 BAP(0.1, 0.2, 0.3)+IAA(0.1)

Hormonal MediaM1

MS MediaM2

MS mediaM3

MS Media

BAP mglˉ¹ NAA mglˉ¹ BAP mglˉ¹ IAA mglˉ¹

( control )

100 % MS Media

0.1 0.1 0.1 0.1

0.2 0.1 0.2 0.1

0.3 0.1 0.3 0.1

Antioxidant Activity

Take Dry leaves powder of 0.5g and add 5ml of different solvents (Methanol, Acetone, Ethnol, Methyl Accetate,Petrolium Ether, Hexane etc)

Take different concentration (5,10,15, 20, 25µl) of extract in test tubes.

Dissolve each concentration in 1000µl methanol and take 25µl in each test tube

Dissolve DPPH in methnol and add 2.7ml per test tube

Cont…..

Keep in Dryer at 37º C for half an Hour.Check the sample on

spectrophotometer at 517nm keeping methnol as blank in opposite cavette

Compare values by formulae Control-Test samples × 100 control (Control: 1.092)

RESULTS

PARAMETERSOptimization For Disinfectant Optimization For Growth Hormones Antioxidant Activity of lavender Multiplication Of Lavender

Effect Of Sodium HypochloriteOn survival of Lavender Cutting

Survival of Plants as result of surface sterilization

Percentage data of plants that survive in different treatment of hormonal media

Effect of Hormonal treatment on number of leaves

Number of shoots observed in Different hormonal media

Shoot Length obtained as result of different treatment

Anti Oxidant Activity

MULTIPLICATION

CONCLUSION

Surface sterilization (5% NaOCl) and micropropagation of lavender by cutting in media containing BAP(0.3mglˉ¹)+ NAA(0.1mglˉ¹) is optimum for acquired plants

• Different combinations of MS media as well as different hydroponics media can also be tested.

• Other than stem cuttings, leaf and root cuttings can also be experimented

• Due to antioxidant activity of lavender it should be used in drug development for cancer patients

• Acquired plants should be investigated for their other medicinal properties

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