In-vitro evaluation of Antioxidant and Anti-diabetic activity of leaf extract of Kigelia africana
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Transcript of In-vitro evaluation of Antioxidant and Anti-diabetic activity of leaf extract of Kigelia africana
1
IN-VITRO EVALUATION OF ANTIOXIDANT AND ANTI-DIABETIC ACTIVITY OF KIGELIA AFRICANA
Sreejith P S (15MSB0045), Majesh Mathew (15MSM0070).
Research Guide: Dr. Devi Rajeswari V
Assistant Professor (Sr),School of Biosciences and Technology,
VIT University, Vellore-14.
13SETMSB1300180
ABSTRACT
• Aqueous leaf extract of medicinal plant Kigelia africana were screened for :
Phytochemical analysis
DPPH radical scavenging activity
Total Antioxidant activity
Hydrogen peroxide scavenging activity
In-vitro Anti-diabetic acitivity
Non enzymatic glycosylation hemoglobin assay
Inhibition of alpha amylase assay
Glucose uptake by yeast cells
INTRODUCTION• Diabetes – A gathering of metabolic illnesses described by hyperglycemia coming about because
of imperfections in insulin discharge, insulin activity, or both.
Causes: Diabetic ketoacidosis, coronary illness, stroke, unending kidney disappointment, foot
ulcers and harm to eyes.
• Antioxidants are involved in prevention of cellular damage- Cancer, aging and variety of
diseases.
• Medicinal plants assume a vital part in the administration of diabetes mellitus particularly in
creating nations where assets are pitiful.
• The antioxidant activity of Kigelia africana was evaluated by measuring reducing ability, free
radical scavenging activity by DPPH and hydrogen peroxide methods.
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METHODOLOGY
Sample collection Extraction process Phytochemical analysis
Antioxidant activityAnti –diabetic activity
Sample collection
Kigelia africana leaves
Extraction process
RESULTS AND DISCUSSION:
1. Phytochemical analysis
• Qualitative analysis
S.no Phytochemical tests
Qualitative analysis
Results
1. Alkaloids Wagner’s test - 2. Carbohydrates Benedict,s test - 3. Proteins and
aminoacid Millions test -
4. Glycoside Borntrager’s test ++ 5. Saponins Foam test +++ 6. Steroids and sterols Salkowski test ++ 7. Phenolic Lead acetate test +++ 8. Tannins Ferric chloride test +++
9. Flavanoids Sodium hydroxide test
++
2. Antioxidant activity:
DPPH radical scavenging activity
3. Total Antioxidant activity:
4. Hydrogen peroxide scavenging activity:
25 50 75 1000
10203040506070
Hydrogen Peroxide
Aqueous extractAscorbic acid
Concentration in µg/mlAb
sorb
ance
25 50 75 10001020304050607080
Total antioxidant activity
Aqueous extractAscorbic acid
Concentration in µg/ml
Abso
rban
ce
• 6. In-vitro anti diabetic activity• Non enzymatic glycosylation hemoglobin assay
200 400 600 800 10000
1020304050607080
Non Enzymatic Glycosylation of Haemoglobin Assay
tocopherolaqueous
concentration (µg/ml)
%in
hibi
tion
• Inhibition of alpha amylase assay
200 400 600 800 1000010203040506070
Alpha Amylase Inhibition
acarboseaqueous
Concentration (µg/ml)%
inhi
bitio
n
• Glucose uptake by yeast cells
40 80 120 160 20001020304050607080
Glucose uptake by yeast cell (10mM)
acarboseaqueous
Concentration (µg/ml)
% o
f upt
ake
40 80 120 160 2000
1020304050607080
Glucose uptake by yeast cells (5mM)
acarboseaqueous
Concentration (µg/ml)
% o
f upt
ake
Conclusion:
As indicated by all tried method, the cell reinforcement action of Kigelia africana was contrasted and that of Ascorbic
acid, which is an actually happening natural compound with cancer prevention agent movement. All in all, the present
study reports that Kigelia africana concentrate can be a decent premise of cancer prevention agent movement and it
likewise can hinder glucose dispersion, α–amylase action and to enhance glucose take-up action by yeast cells utilizing
as a part of vitro models. The confirmation recommend that the plant might be helpful in dynamic administration of
Type2 Diabetes mellitus. A great deal more examinations are required to clarify whether in-vitro impacts speak to
remedial probability in-vivo models for refining glycemic control in Type 2 Diabetes.
Reference
1. Diagnosis and Classification of Diabetes Mellitus, American Diabetes Association. Diabetes Care 2010
Jan; 33(Supplement 1): S62-S69.
2. Medicinal plants in therapy, Norman R. Farnsworth, Olayiwola Akerele, Audrey S. Bingel, Djaja D. Soejarto, and
Zhengang Guo. Bull World Health Organ. 1985; 63(6): 965–981.
3. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity, Molyneux, P.
Songklanakarin J. Sci. Technol., 2004, 26(2) : 211-219
4. Medicinal plants with potential antidiabetic activity - A review of ten years of herbal medicine research (1990-
2000), Mohamed Bnouham*, Abderrahim Ziyyat, Hassane Mekhfi, Abdelhafid Tahri, Abdelkhaleq Legssyer. Int J
Diabetes & Metabolism (2006) 14: 1-25
5. Alfa-glucosidase-inhibiting activity of some Mexican plants used in the treatment of type 2 diabetes, Adolfo
Andrade-Cetto a,∗, Jaime Becerra-Jimenez ´ a, Rene C´ ardenas-V ´ azquez. Journal of Ethnopharmacology 116
(2008) 27–32
6. R. Sicree, J. Shaw and P. Zimmet., The Global Burden of Diabetes: Diabetes and Impaired Glucose Tolerance:
Prevalence and Projections, Diabetes Atlas, International Diabetes Federation, Belgium, 2003, pp. 27-6.