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Transcript of In The Name Of God Precipitation. Antigen/Antibody Reaction Primary reaction : Immunoassay :Like...
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In The Name Of God
Precipitation
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Antigen/Antibody ReactionPrimary reaction:
Immunoassay :Like Elisa , RIA , IFA
Secondary reaction : Lattice formation like Agglutination , Hemagglutination Complement Fixation, Neutralization ,
Precipitation : soluble antibody reacts with soluble antigen
Tertiary reaction : Detected by affect on tissues or cells.
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Secondary Phenomenon
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precipitation: proteinsSeveral types:Protein precipitationSRID DID
ImmunoElectherophoresis :Counter current IEP Rocket IEP
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SRID plate preparation: (Agarose �+ BUFFER (Ab (Anti IgG ) Punch SamplingIncubation in RT for 24hmeasuring of diameter making of curve Calculation of sample concentration
SRID:Single Radial Immuno diffusion or Mancini
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SRID Plate
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Single Radial Immuno diffusion :
In SRID :34 GAM = Is useful for measuring of IgG , IgA, IgM,C3, C4
Gel is mixed with Ab
Ag is in punch In Single Radial Diffusion: one component is fixed
(e.g. antigen-in this case it is IgG)
As the Ag diffuses into the gel it reacts with the Ab and when the equivalence point is reached a ring of precipitation is formed as
illustrated in Figure
The diameter of the ring is proportional to the concentration of Ag .
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(Double Immuno Diffusion =)DID or
Ouchterlony ImmunodiffusionQualitative2 DiffusionApplication in research laboratoryIs not as a routin method now
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Ouchterlony - Identity
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Ouchterlony – Partial Identity
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Ouchterlony Non-Identity
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Turbidimetery
measuring the amount of light Passing Through the solution.
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Nephelometery
Measures SCATTERED light
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Immunoelectrophoresis
Counter current IEP : Qualitative Rocket IEP: Quantitative
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Thanks For your Attention