Embryonic Development VARIATIONS IN EMBRYONIC GERM LAYERS AND BODY CAVITY.
In situ freezing of embryonic stem cells in multiwell plates
Transcript of In situ freezing of embryonic stem cells in multiwell plates
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unexpected; many homeobox pro- teins have similar DNA binding specificities, and ectopic expression suggests large overlap in target genes, The expression patterns of the genes give few indications to their likely ffmction. Genes regulated by homcotic genes are likcly to govern general morphogenetic pro- cesses such as mitotic rate, mitotic orientation, cell-cell interactions and cuticular differentiationS.
The real power of this approach lies in the combination of bio- chemical techniques and genetic analysis to assess whether candi- date genes isolated are likely to be functional targets in vivo. The method should be generally applicable to the investigation of
developmental systems in other species, and enable the isolation of binding sites of other types of regulatory proteins.
Acknowledgements l.J.J, is a Lister fellow.
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Contributed ~. S. E Chan and MJ. Evans, Department o f Genetics, University o f Camlm'dge. Cambn'dge CB2 3EI~. UK.
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