IMPLA N TABLE M ICRO F LUIDIC INTER FFA CE DE … · ion and PDM led with Tet permeable h y...
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I WIT Hiro ABSTRACT An impl newly develo and a perfusi established a could observe microscopy (T microchannel KEYWORD Implantable d INTRODUC The obser challenges in cell[2], but th tissues. To ad function using DEVICE PR The devic mm in diame height) is sea chemicals fro Tetra-PEG ge treatment. Th chemical bon Figure 1. Bdevice mouIMPLAN TH DRUG oaki Takeha 2 Resea 3 Center for T antable micro oped for the in ion membran device manu e neural cells TPLSM). Fur ls in the devic S device, neural CTION rvation of neu n neuroscience here is still roo ddress these p g a PDMS/Tet RINCIPLE ce, which was eter and 500 μ led with the n om the microc el was steriliz he microchan ds. rain interface nted on a mou a b NTABLE M G PERFU ara 1,2 , Akira 1 School o arch Fellow o Disease Biolo ofluidic device n vivo analysi ne made of T ufacturing pro s in a living m rthermore, we e. cell, two-pho ural cells in th e[1]. We repo om for improv problems, we tra-PEG hydro s designed to μm in thicknes newly designe channels into zed by filtrat nels were sea device with a use skull. (b) S MICROF USION FU MEM Nagaoka 2,3 , and T a f Engineering of the Japan So ogy and Integr e with a funct s of neural ce T etra-PEG gel ocedure fully mouse brain i demonstrated ton laser scan e intact brain orted an impla vement in the have develop ogel hybrid m be implantabl ss (Figure 1). ed high-streng brain tissues. tion and PDM aled with Tet a permeable h Schematic of a FLUIDIC UNCTION MBRANE , Jun Noguc akanori Ichi g, The Univers ociety for the rative Medicin tion of drug p ells. The devic l, which is a y compatible w in good physi d the local del nning microsco of a living an antable micro controllability ped an implan microchannel. le into a certa The bottom o gth biocompati The fabricati MS with micr tra-PEG gel hydrogel memb a microchannINTERF N THRO E chi 3 , Takano iki 1 sity of Tokyo, J Promotion of ne, The Unive perfusion thro ce has a hybri high-strength with the steri iological cond livery of mod opy, brain, tet nimal using T ofluidic devic y of the dose ntable microf ain part of the of the microc ible hydrogel ion procedure rochannel stru by the bindin brane. (a) Sch el in the devic FACE DEV UGH HY ri Akagi 1 , H Japan Science, Japa ersity of Tokyo ough a hydrog id structure of h biocompatib ilization proc dition by two- del chemicals i tra-PEG hydro PLSM is one e for the in v amount and d fluidic device e skull to clos hannels (100 called Tetra-P of the device uctures was s ng of Tetra-P hematic of the e. VICES YDROGE Haruo Kasai an o, Japan gel membrane f PDMS micro ble hydrogel. cess. Consequ -photon laser into brain tiss ogel of the most s vivo analysis delivered spac with a drug se a drilled ho μm width an PEG gel[3] to e is shown in sterilized by PEG gel to P microfluidic L i 3 e has been ochannels . We also uently, we scanning sues using significant of neural ce in brain perfusion ole, is 2.7 d 200 μm o transport Figure 2. autoclave PDMS via interface 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences October 28 - November 1, 2012, Okinawa, Japan 978-0-9798064-5-2/μTAS 2012/$20©12CBMS-0001 189
Transcript of IMPLA N TABLE M ICRO F LUIDIC INTER FFA CE DE … · ion and PDM led with Tet permeable h y...
IWIT
Hiro
ABSTRACT An implnewly develoand a perfusiestablished a could observemicroscopy (Tmicrochannel KEYWORDImplantable d
INTRODUC
The obserchallenges incell[2], but thtissues. To adfunction using
DEVICE PR
The devicmm in diameheight) is seachemicals froTetra-PEG getreatment. Thchemical bon
Figure 1. Brdevice moun
IMPLANTH DRUG
oaki Takeha
2Resea3Center for
T antable micro
oped for the inion membrandevice manu
e neural cellsTPLSM). Furls in the devic
S device, neural
CTION rvation of neun neurosciencehere is still rooddress these pg a PDMS/Tet
RINCIPLE ce, which waseter and 500 μled with the n
om the microcel was sterilizhe microchands.
rain interfacented on a mou
a
b
NTABLE MG PERFU
ara1,2, Akira
1School ofarch Fellow ofDisease Biolo
ofluidic devicen vivo analysine made of Tufacturing pros in a living mrthermore, we e.
cell, two-pho
ural cells in the[1]. We repoom for improvproblems, we tra-PEG hydro
s designed to μm in thicknesnewly designechannels into zed by filtratnels were sea
device with ause skull. (b) S
MICROFUSION FU
MEMNagaoka2,3,
and Taf Engineering
of the Japan Soogy and Integr
e with a functs of neural ce
Tetra-PEG gelocedure fully mouse brain i
demonstrated
ton laser scan
e intact brain orted an implavement in the
have developogel hybrid m
be implantablss (Figure 1).
ed high-strengbrain tissues. tion and PDMaled with Tet
a permeable hySchematic of a
FLUIDIC UNCTIONMBRANE, Jun Nogucakanori Ichig, The Universociety for the
grative Medicin
tion of drug pells. The devicl, which is a
y compatible win good physid the local del
nning microsco
of a living anantable microcontrollability
ped an implanmicrochannel.
le into a certaThe bottom o
gth biocompatiThe fabricati
MS with micrtra-PEG gel
hydrogel memba microchanne
INTERFN THROE chi3, Takanoiki1 sity of Tokyo, JPromotion of ne, The Unive
perfusion throce has a hybri
high-strengthwith the steriiological condlivery of mod
opy, brain, tet
nimal using Tofluidic devicy of the dose ntable microf
ain part of theof the microcible hydrogel ion procedurerochannel struby the bindin
brane. (a) Schel in the devic
FACE DEVUGH HY
ri Akagi1, H
Japan f Science, Japaersity of Tokyo
ough a hydrogid structure ofh biocompatibilization procdition by two-del chemicals i
tra-PEG hydro
PLSM is one e for the in vamount and d
fluidic device
e skull to closhannels (100 called Tetra-Pof the device
uctures was sng of Tetra-P
hematic of the e.
VICES YDROGE
Haruo Kasai
an o, Japan
gel membranef PDMS microble hydrogel.
cess. Consequ-photon laserinto brain tiss
ogel
of the most svivo analysis delivered spac
with a drug
se a drilled hoμm width an
PEG gel[3] toe is shown in sterilized by
PEG gel to P
microfluidic i
L
i3
e has been ochannels . We also uently, we r scanning sues using
significant of neural
ce in brain perfusion
ole, is 2.7 d 200 μm
o transport Figure 2. autoclave
PDMS via
interface
16th International Conference on Miniaturized Systems for Chemistry and Life Sciences
October 28 - November 1, 2012, Okinawa, Japan978-0-9798064-5-2/μTAS 2012/$20©12CBMS-0001 189
EXPERIMEPrior to th
numerical simsimulation resapplicable to
Figure 3. (cross sectiof fluorescExperimenconcentratdeviations.
Figure 2. (a)Photograph hydrogel. Th
a
a
NT he implantati
mulation of thsults showed the well-contr
(a) Schematic on of the micr
cein after the dntally measurtion (CFEM; so.
a) Fabricationof the microf
he device is 2.
on experimenhe flow and di
excellent agrerolled delivery
illustration ofrochannel aftedelivery of 5 μred fluorescenolid lines) at
n process of thfluidic interfac7 mm in diam
d
nts, transient iffusion in theeement, as shoy of chemicals
of Tetra-PEG ger the deliveryμM fluoresceinnce intensity various depth
he device. PDce device. The
meter and 500 μ
b
diffusion expe device usingown in Figures or drugs into
gel/PDMS hyby of 5 μM fluon for 10 min c(IExp.; symbo
hs (50 μm, 1
DMS with mice device is im μm in thickne
periments werg the CFD-ACe 3. Thus, theo the mouse b
brid microchaorescein for 10alculated by t
ols) and comp00 μm, 200 μ
crochannels wmersed in wa
ess.
b
re performed.CE+ software.e device develrain.
annels. (b) Flu0 min. (c) Conthe finite-elemputationally μm). Error ba
was sealed witter to avoid th
c
. We also con
. The experimloped was pro
uorescence imncentration disment method (F
calculated fluars show the
th Tetra-PEG he deformatio
nducted a mental and oved to be
mages of a stribution FEM). (d) uorescein standard
gel. (b) on of the
190
Then, in vwas observeddevice was ssolutions wersubstance in b
CONCLUSIO
We havemembrane ftissues of livin vivo analy
ACKNOWL
This resea REFERENC[1] Helmche[2] Takehara
scanning[3] Sakai, T.
from tetr CONTACT Hiroaki Takeh
Figure 4bars: (a)
Figure 5. (ainject solutifluorescent
a
b
vivo experimed by TPLSM, asufficiently stre injected intbrain tissues w
ON e presented tfor deliveringving animalsysis in the fie
LEDGEMENTarch was supp
CES en, F., et al., Da, H., et al., g microscopy,., et al., Desigrahedron-like
hara, tel: +81-3
4. (a) Bright ) 500 μm and
a) A living moions into the substance in b
a
ents were demand high-resoterilized and to the microchwas measured
the usefulnesg chemicals . Implantableeld of neuros
TS orted by Gran
Deep tissue twMicrofluidic , Proceedings gn and fabricat
macromonom
3-5841-1180; h
image of the (b) 100 μm.
ouse immobilimicrochannelbrain tissues a
monstrated as folution (<1 μm
the pulsatile hannels of theby TPLSM (F
ss of an impin a control
e microfluidiscience and f
nt-in-Aid for J
wo-photon micinterface devof MicroTAStion of a high-
mers. Macrom
h-takehara@bio
brain surface
ized with the ls. (b) Deviceat depths of 25
follows. The bm) microscopy
motion was e device, and Figure 5).
plantable milled manneric device tec
for the discov
JSPS Fellows
croscopy, Natuvices for in vi 2010 confere-strength hydr
molecules, vol.
onano.t.u-toky
e under the d
headgear. Tefe in the headg5 μm and 50 μ
c
b
brain of a liviy images of ne
successfully the diffusive
crofluidic deand for obs
chnology is every of drugs
(22-8063).
ure Methods vivo analysis oence, pp. 2111rogel with ide 41, pp. 5379-
yo.ac.jp
device. (b) Two
flon tubes wergear. Scale baμm. Error bar
ng mouse impeural cells wersuppressed (Ftransfer of th
evice with a erving fine
expected to bs for treating
vol. 2, pp. 932of neural cell-2113 (2010).
eally homogen-5384 (2008).
o-photon imag
re connected tar: 2 mm. (c)rs show the sta
planted with tre obtained be(Figure 4). Flhe delivered fl
a permeable structures of
be a powerfug brain diseas
2-940 (2005). ls using 2-pho. neous network
age of neural
to the device ) Diffusive traandard deviat
the device ecause the luorescent luorescent
hydrogel f cells in
ul tool for ses.
oton laser
k structure
cells. Scale
tubes to ansfer of tions.
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