Immunogenetics Sadegh Babashah, PhD Department of Molecular Genetics, Faculty of Biological...
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Immunogenetics Sadegh Babashah, PhD Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University
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Transcript of Immunogenetics Sadegh Babashah, PhD Department of Molecular Genetics, Faculty of Biological...
ImmunogeneticsImmunogenetics
Sadegh Babashah, PhDDepartment of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University
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History
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Infection begins inflammatory response
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Clonal selection hypothesis
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Schematic representation of Ag receptors
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Isolation of TCR molecule
1. Production of Mab against various T-cell clones
2. Screening these antibodies against different T-cell clones
3. Finding clone-specific Mab
4. Isolation of TCR by clone-specific Mab
A. Monoclonal antibody (Mab)
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B. Cloning TCR genes
1. Isolation of mRNA from T-cell
2. Synthesis of cDNA
3. Hybridization of cDNA with B-cell mRNA for Isolation of T-cell specific cDNA by
4. Southern blotting with genomic DNA for finding rearranged cDNA
5. Rearranged cDNA is TCR gene
Isolation of TCR molecule
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Production ofLymphocyte Ag receptors
(BCR, TCR)
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Recombinase complex involved in V(D)J recombination:
- Lymphocyte-specific recombinase (RAG-1 & RAG-2)
- General enzymes (e.g. Ku heterodimer)
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The Non-homologous End-Joining repair Process (NHEJ)
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T-cell receptor
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α and β locuses in T-cell receptor gene
TCRα:
TCRβ:
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Rearrangement in T-cell receptor gene
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تنوع ترکیبی + تنوع تلفیقی
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TCRα:
TCRβ:
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TCRα:
TCRβ:
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TCRα (containing TCRδ):
TCRγ:
δ-chain locus………..…..
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TCRα (containing TCRδ):
δ-chain locus………..…..
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Pantameric IgM
Dimeric IgA
x 900’000
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Thank you for listening, any comments?
