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ImmunoassayResource Guide

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Table of Contents1. Introduction 1

2. Immunoassay technology 2

3. Immunoassay platforms 3

4. Coat-it-yourself 4

Ready-SET-Go!® ELISA . . . . . . . . . . . . . 4

5. Pre-coated 6

Platinum ELISA . . . . . . . . . . . . . . . . . 6

High Sensitivity ELISA. . . . . . . . . . . . . . 8

6. One-wash ELISA 10

Instant ELISA® kits . . . . . . . . . . . . . . . 10

InstantOne ELISA™ kits . . . . . . . . . . . . . 12

7. Multiplex 14

ProcartaPlex® Immunoassays . . . . . . . . . . 14

High Sensitivity ProcartaPlex® Immunoassays . . 23

Platinum ProcartaPlex® Immunoassays . . . . . 24

8. Affymetrix Services 25

9. ELISA troubleshooting 26

10. Immunoassay products 29

1

Introduction

The accurate assessment of disease risk potential is significantly improved by disease-

defined biomarker profiling. Research to gain greater insight into these biomarker

profiles will ultimately produce a better understanding of disease, its risk potential, and

possible treatments. eBioscience produces multiple platforms for analyte assessment and

biomarker profiling—from coat-it-yourself Ready-SET-Go!® ELISAs and traditional Platinum

ELISAs, to ProcartaPlex® Multiplex Immunoassays, using Luminex xMAP® technology.

Plate- and bead-based solutions for your research needs across six species Human Mouse Rat Non-human Primate Canine Porcine

eBioscience immunoassays support the evolution of research projects by offering multiple

platforms that, in most cases, use the same antibody content. This provides freedom to

move from one platform to another or from a multiplex assay to an ELISA, producing truly

comparable data.

�Keep your research relevant by incorporating analytes pertinent to your area of research

at any point within your study.

�Conserve resources by using existing data from previous experiments.

�Revalidate at your discretion with identical antibody content across multiple platforms.

Immunoassays within these areas of research assess cellular mechanisms and disease states in basic discovery to drug development

Adaptive immunity

Adhesion and migration

Angiogenesis

Apoptosis

Bone biology

Cancer biology

Cell biology

Cell cycle and proliferation

Cell signaling

Cell structure

Cytokines, chemokines, and growth factors

Immunology

Innate immunity

Metabolism

Stem cell biology

Transcription factors

2

Immunoassay technologyWhat are ELISAs and multiplex immunoassays?

Both ELISAs and multiplex immunoassays are routinely used for the qualitative and quantitative assessment of cytokines,

chemokines, growth factors, phosphorylated targets, immunoglobulins, and other immunological markers. Detection

and quantitation of protein analytes from various biological samples, including serum, plasma, and other bodily fluids

(e.g., sputum, urine, etc.) as well as cell culture supernatants and cell lysates, indicate a multitude of biological and

pathological events. Immunoassays are used in areas of basic research for studying cell transformation and activation.

They have also proven to be valuable tools in drug development as they are commonly used for biomarker profiling of

disease versus normal states, as well as toxicity profiling.

eBioscience ELISAs and multiplex immunoassays use the sandwich immunoassay technique of two highly specific

antibodies that recognize two different epitopes on the same protein. This provides an extra layer of specificity

over competitive ELISA formats that employ only the use of a single antibody. To understand the advantages of

sandwich immunoassays, it is important to understand the basics of the assay.

(1) (2) (3) (4) (5)

Capture Ab

Biotin Streptavidin

TMBSubstrateHRP

Target Protein

DetectionAb

Sandwich ELISA workflow diagram

1. The first antibody, specified as the capture antibody, is bound to either the well of the ELISA plate or to a capture bead in a ProcartaPlex Multiplex Immunoassay.

2. The sample of choice is incubated in a pre-coated ELISA well, or in the case of a ProcartaPlex assay, in a plate well with the capture bead mixture. During this incubation step, the specific antigen is captured on the solid surface (plate well or bead) through its specific binding to the capture antibody. The remaining sample is washed away.

3. A second biotinylated or direct horseradish peroxidase (HRP)-labeled, analyte-specific antibody is used for analyte detection. The detection antibody adds a layer of specificity for the sandwich immunoassays. Any unbound detection antibody is washed away.

4. A detection reagent used in the sandwich immunoassays allows for the detection and quantitation of the analyte. For ELISAs, the use of a horseradish peroxidase (HRP)-labeled, analyte-specific antibody triggers the colormetric conversion of the substrate and, hence, the development of color. For the ProcartaPlex assays, the detection reagent is a streptavidin-phycoerythrin (streptavidin-PE) conjugate, which allows the instrument to quantitate the analyte levels based on fluorescence intensity.

5. High-antigen concentration causes more substrate to be converted, which produces a stronger colorimetric signal for the ELISA and higher fluorescent readings for ProcartaPlex assays. Correlation of the color development or fluorescence intensity can then be used to calculate the concentration of an antigen in a sample when compared to a standard curve of known concentration.

3

Immunoassay platformsTailored to meet your needs

Immunoassay components Ready-SET-Go!® ELISA

Platinum ELISA

High sensitivity

ELISA

Instant ELISA® kit

InstantOne ELISA™ kit

ProcartaPlex® assays

(all platforms)

96-well Plate optional § § § InstantOne plate Flat bottom

Pre-titrated Capture Antibody § Pre-coated Pre-coated Pre-coated §Pre-coated on capture beads

Pre-titrated Detection Antibody § § § § § §

Recombinant Protein Standard § § §Additional well strips included

Control cell lysate §

Plate Sealers § § § § §

Manual § § § § § §

Stop Solution § § § § §

Substrate Solution § § § § § SA-PE

Assay Buffer § § §Sample diluent

lyophilizedCell lysis buffer & enhancer solution

§

Wash Buffer § § § § §

Coating Buffer § §

Note: eBioscience immunoassays are available in various kit sizes. Kits are calibrated to NIBSC/WHO Standards (if available). SA= streptavidin.

Customized immunoassay development In addition to our immunoassay sets and kits, eBioscience offers contract assay development services and the expertise to provide the comprehensive solutions your research requires.

Ready-SET-Go!® ELISA – Keep costs low. Each affordable ELISA set for cost-it-yourself plates includes the reagents required to prepare and run the ELISA, including: ELISA-optimized antibody matched pairs, standards, detection reagents, coating buffers, sample diluent, and TMB substrate solution. Plates are optional.

Platinum ELISA – Quantitate with confidence. Highly validated ELISA kits with pre-coated plates provide lower inter- and intra-assay variability with ready-to-use reagents that ensure consistent data throughout your research.

High Sensitivity ELISA – Detect low-abundant cytokines. Amplified sandwich ELISAs allow for detection limits below 1 pg/mL without loss of resolution or increase in background. The addition of sample is all that is required to start the assay.

Instant ELISA® kits – Reduce steps and hands-on time to increase productivity. The one-wash ELISA introduces fewer handling steps, leaving more time for your research.

InstantOne ELISA™ kits – Save time and reduce steps. Our one-hour, one-wash cell signaling assay detects total and phosphorylated targets with sensitivity exceeding industry standards.

ProcartaPlex® Multiplex Immunoassays – Quantitate more with less sample. ProcartaPlex Multiplex Immunoassays utilize Luminex® xMAP® technology for the biomarker profiling of up to 80 analytes in a single 25–50 μL sample.

ProcartaPlex® High Sensitivity Immunoassays – Take a closer look into your sample and detect concentrations in femtogram range. High-Sensitivity ProcartaPlex Immunoassays offer accurate quantification of key cytokines significantly below the detection limit of conventional Luminex® assays.

Platinum ProcartaPlex® Immunoassays – Overcome matrix effects. Platinum ProcartaPlex Immunoassays are our most validated bead-based multiplexing assay system.

4

Capture AntibodyDetection Antibody

Coating Bu�er Assay Diluent

Detection Enzyme Substrate Solution

ELISA Plate (optional)

Ready-SET-Go!® ELISA kit components

Ready-SET-Go!® ELISAs offer 2-, 10-, and 20-plate (96-well) kit sizes. Kits include all reagents necessary to perform the assay (with the exception of acid stop solution and wash buffer). Plates (96-well) are optional with the 10- and 20-plate kit sizes.

Coat-it-yourself

Ready-SET-Go! ELISA Optimized reagents–all in one box

Keep costs low. Affordable ELISA kits using coat-it-yourself plates also include the

necessary reagents to prepare and run an ELISA, including ELISA-optimized antibody

matched pairs, standards, detection reagents, wash buffers, and coating buffers (plates

are optional).

Ready-SET-Go! ELISAs are ideal for experienced ELISA users on a budget, who have less

stringent requirements for inter- and intra-assay variance.

Standard curve for human CD163 Ready-SET-Go!® ELISA.

Human CD163 Ready-SET-Go!® ELISA

Concentration ng/mL

Ab

sorp

tio

n 4

50 n

m

5

Advantages of Ready-SET-Go! ELISA

�Flexible: Purchase based on the volume of your research through a choice of package sizes, including 2-, 10-, and 20-plate kits.

�Complete and easy to use: Includes optimized antibody pairs and recombinant standards. Unlike ELISA sets from other suppliers, Ready-SET-Go! sets also include TMB substrate and all the essential reagents and buffers to perform your assay.

�Affordable: Priced to accommodate the most demanding budgets and to maximize your research.

Ready-SET-Go!® ELISA sample protocol

§ Wash unbound capture antibody from well.

2

§ Add HRP-conjugated Streptavidin.

§ Incubate 30 minutes.

8

§ Add colorimetric detection reagent.

§ Let develop (~10–30 minutes.).

10

§ Wash unbound, non-specific material from well.

5

§ Wash unbound detection antibody from well.

7

§ Wash unbound HRP-conjugated Streptavidin from wells.

9§ Stop the colorimetric reaction

with addition of Stop Solution.

§ Read in plate reader at 450/650 nm.

11

§ Add biotinylated detection antibody.

§ Incubate for one hour.

6

Refer to individual product manuals for performance data.

§ Add sample.

§ Incubate for two hours.

4

§ Capture antibody diluted in coating buffer overnight at 40C.

1

Coat-it-yourself

3

§ Block with blocking buffer.

§ Incubate at room temperature for two hours or overnight at 40C.

6

Platinum ELISA kit components

DetectionAntibody

StandardProtein

Ready-to-useassay reagents

Pre-coated ELISA Plate

Platinum ELISA kits are available in 1- and 10-plate (96-well) kit sizes. Kits include all reagents necessary to perform the assay.

Platinum ELISA A complete, validated ELISA solution

Quantitate with confidence. Platinum ELISA kits for the quantitation of individual

analytes from a variety of sample types provide lower interand intra-assay variability.

Ready-to-use reagents including pre-coated plates ensure consistent data throughout

your research. Developed and manufactured for labs that require validated and

comprehensively tested kits, Platinum ELISA kits guarantee lot-to-lot consistency and

ensure the highest possible ELISA performance.

Pre-coated

Standard curve for human IgG1 Platinum ELISA.

Concentration ng/mL

Human IgG1 Platinum ELISA

Ab

sorp

tio

n 4

50 n

m

7

§ Add HRP-conjugated Streptavidin.

§ Incubate one hour.

3

§ Add colorimetric detection reagent to each used assay well in the microplate.

§ Let develop (~10–30 minutes).

5

§ Wash unbound detection antibody from well.

2

§ Wash unbound HRP-conjugated Streptavidin from wells.

4

§ Stop the colorimetric reaction with addition of stop solution.


6

§ Add sample and biotinylated detection antibody.

§ Incubate for one hour.

1

Platinum ELISA assay workflow

Refer to individual product manuals for performance data.

Advantages of Platinum ELISA

�Robust: Low inter- and intra-assay coefficient of variation (CV)

�Easy-to-use: Includes all required (color-coded) assay buffers and reagents and a

straightforward protocol

�Highly validated: Optimized for serum, plasma, and cell culture supernatants

Pre-coated

8

High Sensitivity ELISA Kits for human and mouse species

Detect low abundant cytokines. The amplified sandwich ELISA offers detection limits

below 1 pg/mL without loss of resolution or increase in background.

Look beyond the conventional boundaries of detection

High Sensitivity ELISA Kits offer accurate quantification below conventional ELISA limits.

This is made possible through patented signal amplification technology, with negligible

effects on background signal. The amplification signal builds upon the traditional HRP

enzymatic colorimetric detection to allow for an additional log of sensitivity.

Pre-coated

High Sensitivity ELISA kit components

Standard Detection

Wash Bu�erSubstrate Solution

Sample Diluent Assay Bu�er

Amplifying Reagents

Pre-coated Plate

Concentration pg/mL

0.5

1.0

1.5

2.0

2.5

0.0

0 10080604020

Ab

sorp

tio

n 4

50 n

m

Standard Curve IL-6

High Sensitivity ELISA

Traditional ELISA

Human IL-6 quantitation comparison using both the

conventional approach (Platinum ELISA) and the High Sensitivity

ELISA assay.

High Sensitivity ELISA (1 x 96-well plate) kit includes all reagents necessary to perform the assay.

9

Advantages of High Sensitivity ELISA

�Sensitive: Detect low cytokine concentrations (≤1.0 pg/mL) for reliable quantification.

�Minimal sample required: Uses 50 µL sample volume, making it ideal for limited resources.

�Easy-to-use: Includes all required reagents and a straightforward protocol.

�Compatible instrumentation: Uses standard colorimetric plate reader (absorption measurement at 450 nm).

High-sensitivity technology

eBioscience High Sensitivity ELISAs detect low expressing proteins by enhancing the

signal obtained from conventional colorimetric ELISAs. A conventional sandwich ELISA

signal development is accomplished only via the biotin/streptavidin-HRP amplification

system. eBioscience High Sensitivity ELISAs further enhance this signal with the additional

signal amplification step using a tyramide amplification reagent to generate multiple

biotin binding sites for additional streptavidin-HRP molecules. The amount of reacted

biotinyl-tyramide is proportional to the amount of HRP in the well. This amplification step

effectively multiplies the quantity of HRP molecules for the final TMB substrate reaction.

DetectionSubstrate

Streptavidin

Biotinyl Tyramide

HRP

Biotinylated Detection Antibody

Antigen

Absorbance Reading

Key

Capture Antibody

High Sensitivity ELISAStandard ELISA

Accurately measure low abundance targets. Robust and sensitive assays for human and mouse targets. eBioscience has successfully applied the Tyramide Signal Amplification technology, which enhances the sensitivity of a conventional pre-coated, plate-based ELISA to reach concentrations ≤1.0 pg/mL.

High Sensitivity ELISA is based on a patent from PerkinElmer LAS, patent number 5,731,158;5,583,001;5,196,306.

Pre-coated

10

Instant ELISA kits The one-wash ELISA

Reduce steps and hands-on time to increase productivity. The one-wash Instant

ELISA requires fewer handling steps. Less hands-on time increases productivity, which

leaves more time for your research.

Instant ELISA® (1 x 96-well plate) kit includes all reagents necessary to perform the assay. Two separately packed, double-standard curves come with kits, which enables use of all 96-wells for your samples.

Instant ELISA® kit components

Wash Bu�erSubstrate Solution

Assay Bu�erStop Solution

Ready-to-useStandard Curve

Capture & DetectionAntibodies Lyophilized

to the 96-strip Well

Standard Samples

17 Steps for Conventional ELISA 7 Steps for Instant ELISA® kits

01 Washing of coated plate

02 Reconstitution of standard protein

03 Addition of sample diluent to

standard wells

04 Titration of standard curve 01 Rehydration of plate

05 Addition of sample diluent 02 Sample addition

06 Sample addition 03 Incubation

07 Dilution of biotin conjugate

08 Addition of biotin conjugate

09 Incubation

10 Preparation of streptavidin-HRP

11 Washing step

12 Addition of streptavidin-HRP

13 Incubation

14 Washing step 04 Washing step

15 Addition of TMB substrate 05 Addition of TMB substrate

16 Addition of stop solution 06 Addition of stop solution

17 Calculation of results 07 Calculation of results

One-wash ELISA

11

One-wash ELISA

Switching to Instant ELISA kits

eBioscience immunoassays utilize the same antibody pairs for most analytes across all

assay formats. This enables cross-platform performance and comparable data using any

of our Instant ELISA kits, Platinum ELISA and ProcartaPlex® multiplex assays. For example,

if you are currently using a sCD44var Platinum ELISA, you can be confident in switching

to the sCD44var Instant ELISA assay, knowing that the data generated across the two kits

will remain consistent.

Advantages of Instant ELISA Kits

�Time saving: Only requires 15 minutes for setup.

�Maximum accuracy: No need to add antibody or do serial dilution of standards; reduced handling means less error and more consistent results.

�Better value: Generate standard curve data in parallel with additional well strips provided to enable use of all 96 wells for your samples.

Performance comparison of human sCD44var Instant ELISA® to

Platinum ELISA

Human sCD44var Instant ELISA® kit was tested against a human sCD44var Platinum ELISA kit. Sixteen serum samples were applied with the coefficient of determination of 0.98, demonstrating comparable performance of the Instant ELISA® kits against Platinum ELISA kits.

sCD44var Instant ELISA® kits

sCD

44va

r Pl

atin

um

ELI

SA

0 10 20 30 40 50 60 70 80

0 10 20 30 40 50 60 70

R2=0.98

12

InstantOne ELISA kits Phospho cell signaling assay

Easy one-hour protocol. InstantOne ELISA, the one-hour, one-wash ELISA for cell

signaling, detects total and phosphorylated targets with an easy-to-use assay that results

in sensitivity that exceeds industry standards.

The one-hour, one-wash phospho ELISA

InstantOne ELISAs use the traditional sandwich ELISA format with some significant

differences that allow for greater flexibility, ease of use, reduced assay time, and reduced

hands-on assay time. Unlike a typical sandwich ELISA, the target analyte binds to the two

sandwich ELISA antibodies in solution. As the capture antibody binds to the plate through

a proprietary mechanism, both the sample and the assay reagents may be added to the

InstantOne ELISA assay plate at the same time. Unbound assay reagents and non-specific

sample components are washed away (just as in a traditional sandwich ELISA), while the

specific analyte is detected through a colorimetric detection. The entire process takes a

little more than one hour to complete. Analysis of both total and phosphorylated targets

across family members or down pathways on the same plate has never been easier.

In addition to the simplicity the InstantOne ELISA provides, it also adds a layer of flexibility

not readily accessible with traditional sandwich ELISAs. Since the antibodies are not pre-

coated in the wells, several different targets can be analyzed simultaneously on the same

plate in different wells. Simply add the sample lysate to the plate wells and add different

antibody reagent cocktails to the different wells.

InstantOne ELISA™ kit components

CaptureAntibody

PositiveControl

Cell Lysis Bu�erEnhancer Solution

Wash Bu�erTMB SubstrateStop Solution

InstantOne 96-well Strip

One-wash ELISA

InstantOne™ ELISA (1 x 96-well plate) kit includes all reagents necessary to perform the assay.

13

ELISA and Western blot using the same antigen-specific antibodies (EKR 1/2)

InstantOne™ ELISA one-hour protocol

A431 cells were treated with 10 μM AG1478 (an EGFR kinase inhibitor) for three hours (–) or treated with 10 ng/mL EGF for 10 minutes (+). Analysis: 10 μg/analysis Western blot, 5 μg/analysis InstantOne ELISA™ kit.

[A431 lysate] ng protein/lane

phospho-ERK 1/2 (Thr202/Tyr204) Titration

[A431 lysate] ng protein/well35

0017

50 880440

220

110 55 3400 1700 875 440 220 110 55 27.5 13.8

+ + +

p-ERK1 Total ERK1Total ERK2p-ERK2

– – –

phospho-ERK 1/2 (Thr202/Tyr204) ERK 1/2 (total)

+ –

Advantages of InstantOne™ ELISA

�Fast: Less than a third of the time of a traditional ELISA

�Easy: Only one wash step, eliminating hands-on time

�Flexible: Enabled detection of both total and phosphorylation protein targets up and

down a pathway or across family members on a single plate

�Sensitive: Exceeding industry standards, with only 2,000 to 3,000 cells needed

�Compatible: Conventional colorimetric readout

§ Wash microplate with included wash buffer.

§ Add lysates to the InstantOne assay microplate.

§ Add Antibody Cocktail containing the capture, detection, and enhancer solution to the microwell.

§ Incubate wells for one hour.

Sample in-cell lysis mix

Antibody Cocktail mix

1 2

§ Stop the colorimetric reaction with addition of stop solution.


4

§ Add colorimetric detection reagent to each of the used assay wells in the microplate.

§ Let develop (~10–30 minutes).

3

One-wash ELISA

14

ProcartaPlex Multiplex Immunoassays

Profile more biomarkers with less sample.

ProcartaPlex Multiplex Immunoassays use the Luminex xMAP (multi-analyte profiling)

technology that enables the simultaneous detection and quantitation of up to 80 protein

targets in a single 25–50 µL sample of plasma, serum, cell culture supernatants and other

bodily fluids. The technology employs the use of differentially dyed capture beads for

each target in a multiplex “ELISA-like” assay. ProcartaPlex immunoassays can profile up

to 80 times more analytes using significantly less sample in the same time that it takes to

perform a traditional sandwich ELISA.

ProcartaPlex Multiplex Immunoassays provide the ability to create your unique panel and the

assurance that you will achieve reproducible results throughout the course of your study.

�More than 90% of ProcartaPlex assays can be combined with one another

�High correlation to our Platinum ELISA assays (R2>0.9)

�Scalable and reproducible performance regardless of plex size

�Largest published panel for quantitation of up to 63 analytes1,2,3,4

ProcartaPlex Multiplex Immunoassays are available in multiple formats across six species

(human, mouse, rat, non-human primate, porcine and canine) to meet the needs

of your research.

�ProcartaPlex® Panels – Pre-configured, biologically relevant and disease-defined panels

using magnetic beads for the quantitative multiplex analysis of up to 80 analytes in a

single sample.

�ProcartaPlex® Simplex Kits – Magnetic bead sets for the detection of individual analytes

designed to be added to ProcartaPlex Panels for increased customization. Alternatively,

multiple Simplex Bead Sets can be combined and run using the ProcartaPlex® Basic Kit,

which includes all non-target specific reagents needed to perform the assay.

�ProcartaPlex® Mix&Match Panels – Custom-blended and optimized panels deliver

results tailored to the panel design of YOUR choice. Simply provide your desired species,

sample type and instrument for use. Then select your desired analytes, and a custom

assay kit will be built and optimized to your specifications.

Multiplex

Visit ebioscience.com/ProcartaPlex for information regarding characteristic assay details and individual analytes.

15

Multiplex

ProcartaPlex® assay workflow

Read & Analyze

Figure 4

MFI

Concentration (pg/mL)

IL-6 (25)

IL-10/CSIF (28)

IL-5 (21)

IL-12/IL-23p40 (33)

MIG/CXCL9 (57)

1

1

10

10

100

100

1000

1000

10000

10000

100000

100000

Secreted proteins simultaneously measured using ProcartaPlex® Cytokine Assay.

Read with Luminex instrument

ProcartaPlex® Analyst Software

For analyte quantitation, the immuno (sandwich) complex is incubated with a fluorescent detection label (SA-PE)

The sample is then washed to remove excess SA-PE

Multiplex sample is ready for quantitation

Label detection

Figure 3

Incubate 30 min and wash

Capture target analytes

Figure 1

Incubate 1–2 hours and wash

Analyte-specific capture beads are added to prepared sample. The unique, differentially dyed capture beads are coated with target-specific capture antibodies. This allows for the unique identification of the analyte.

Detect captured analyte

Figure 2

Incubate 30 min and wash

Captured bead/analytes are incubated with biotinylated analyte-specific detection antibody to form an immuno (sandwich) complex.

Capture Beads

Analyte

Detection Antibody

Incubation / Wash

SA-PE

16

Why is the assay Luminex-based?

Profile more biomarkers with less sample amount

Quantitate up to 80 analytes simultaneously with the Luminex 100/200™ or FLEXMAP 3D®

and up to 50 analytes with the MAGPIX®. All of our assays are compatible with as little as

25 µL of plasma or serum samples or 50 µL of cell culture supernatant sample.

High-throughput analysis

Measure several analytes simultaneously in the same time it takes to run a single ELISA assay.

ProcartaPlex protocols are available in 96-well and 384-well formats for high-throughput analysis.

Trusted platform and partnership

Luminex has been supplying instruments to the life science research market for almost 20 years

and has over 13,000 units installed worldwide. As a certified partner of Luminex, Affymetrix

has been supplying multiplexed reagents for nearly 10 years and has been a preferred supplier

of high quality assays to many customers in medical and drug discovery research.

ProcartaPlex® technology overview

xMAP technology ProcartaPlex assays use xMAP magnetic bead technology that uses the Magplex™

superparamagnetic 6.5-micron microsphere beads with a magnetic core. The beads

are internally dyed with precise proportions of red and infrared fluorophores. Varying

proportions of the red and infrared fluorophores result in 100 spectrally unique

microspheres, which are identified by the Luminex xMAP detection systems. The

conjugation of a distinct monoclonal antibody to a distinct bead allows for analysis of

multiple analytes in a single well.

Luminex instrumentation Luminex instrumentation is comprised of three instrument models: MAGPIX,

Luminex100/200 and FLEXMAP 3D. These instruments are based on either the principles

of quantitative fluorescent microscopy or fluorescent flow cytometry. The multiplex

quantitation capability of an assay is dependent upon instrument model and ranges from

50 analytes with the use of the MAGPIX, to 500 analytes with the FLEXMAP 3D. Used in

combination with ProcartaPlex assays, the Luminex platform delivers fast and cost-effective

immunoassay profiling analysis. To learn more detailed information about the different

instrument types, visit our website under Luminex Instruments.

The bead is impregnated with the dye mixture

Multiplex

17

Why ProcartaPlex assays?

Flexibility

Get exactly the panel you want because more than than

90% of ProcartaPlex assays are combinable with one

another. ProcartaPlex assays allow the creation of highly

individual and high-plex panels. Affymetrix currently offers

the largest published panel with a 63-plex.1,2,3,4

Reproducible results

ProcartaPlex assays are based on the principles of sandwich

ELISA where two highly specific antibodies recognize

different epitopes on the same protein. Reproducible

results come from reliable manufacturing. Affymetrix

owns and operates a world-class hybridoma facility for the

manufacturing of antibodies, and these antibodies are used

in ProcartaPlex assays, enabling the delivery of consistent,

reproducible, and high-quality assays.

Correlation to ELISA

Switch easily from ProcartaPlex assays to ELISA and vice-

versa with reliable results. A majority of our ProcartaPlex

assays use the same antibody pairs as our Platinum

ELISA, resulting in high correlation (R2>0.9) between

the two assays. As shown in Figure 1, equivalent results

(R2>0.9) were obtained using the ProcartaPlex Multiplex

Immunoassays and traditional plate-based ELISAs for

human and mouse IFN gamma. ProcartaPlex assays achieve

quantitative results comparable to traditional ELISA results

with the added benefits of reduced sample volume, shorter

assay time, and lower cost.

Results of an experiment measuring IFN gamma and TNF alpha in stimulated human and mouse PBMCs. Supernatants were serially diluted two-fold in normal human or mouse serum, to allow for differential cytokine levels in a serum matrix. Additional cytokines for human and mouse analytes were compared including IL-beta and IL-17A, and similar performance was obtained (data not shown).

Multiplex

R² = 0.96576

Human TNF alpha ProcartaPlex®/ Platinum ELISA Correlation

Pro

cart

aPle

x® a

ssay

[p

g/m

L]

Platinum ELISA [pg/mL]

R² = 0.9733

Human IFN gamma ProcartaPlex®/ Platinum ELISA Correlation

Pro

cart

aPle

x® a

ssay

[p

g/m

L]

Platinum ELISA [pg/mL]

Pro

cart

aPle

x® a

ssay

[p

g/m

L]

Mouse IFN gamma ProcartaPlex®/ Platinum ELISA Correlation

Platinum [pg/ml]

R² = 0.98201

Pro

cart

aPle

x® a

ssay

[p

g/m

l]

Platinum [pg/mL]

Mouse TNF alpha ProcartaPlex®/ Platinum ELISA Correlation

R² = 0.99311

18

ScalabilityThe ability to scale the number of analytes investigated in multiplex experiments is critical for the progression of many projects. For example, it is not uncommon to begin a project by analyzing a large, comprehensive 35-plex cytokine panel on fewer samples (i.e., 100) to determine which analytes are affected by a particular disease or drug treatment. The resulting list of analytes can then be investigated further, for instance, using an 11-plex panel, on a larger set of samples. Thus, there is a great need for scalability in multiplex panel assays in order to enable researchers to correlate all their data across the different stages of their investigation. Many commercially available multiplex assays have not been developed with this level of consistency in mind. However, this specification is a key design requirement incorporated during the ProcartaPlex assay development. Data in Figures 2 and 3 illustrate the high correlation between similar analytes in a large and small panel and the scalability when using ProcartaPlex assays. Similar results are obtained when investigating other analytes.

Scalability with Consistency

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

Mouse IFN gamma ProcartaPlex® 26plex vs Th1/Th2 11plex

Pro

cart

aPle

x® T

h1/

Th2

11p

lex

[pg

/mL]

ProcartaPlex® 26plex [pg/mL]

R² = 0.9995

Figure 2: Data illustrates the high correlation between large- and small-scale multiplex scaling when using the ProcartaPlex® assays. Regression analysis comparing the ProcartaPlex® Mouse Cytokine & Chemokine Panel 1 (26 plex) and the ProcartaPlex® Mouse Th1/Th2 Cytokine Panel (11 plex) revealed an R2 value >0.9995.

Figure 3: To illustrate the scalability of ProcartaPlex® Multiplex Immunoassays, diseased human serum samples were run in parallel using both the ProcartaPlex® Human Cytokine and Chemokine Panel 1A (34 plex) and the Human Th1/Th2 Cytokine Panel (11 plex). Experiments demonstrate consistency achieved in both large- and small-scale multiplex panels.

Free analysis softwareProcartaPlex® Analyst Software is easy-to-use yet powerful. It is compatible with *.csv file output from the Luminex instrument and can be downloaded at www.ebioscience.com/resources

ProcartaPlex Analyst Software provides

n 4PL or 5PL standard curve evaluationn Linear, logarithmic, and point-to-point fitn Pre-defined dilution factor and standard rangen Export settings for fast and easy re-analysisn Export graph images for publishingn At-a-glance sample pattern with a heat map

Five-parameter curve fitHeat map analysis for sample concentration

pg

/mL

5,000

4,500

4,000

3,500

3,000

2,500

2,000

1,500

1,000

500

0

14.0

12.0

10.0

8.0

6.0

4.0

2.0

0.0

0 2,000 4,000 6,000

Multiplex

19

Create your own custom panelBuild the panel of your choice with our online Panel Configurator at www.ebioscience.com. Select your analytes of interest and immediately receive recommendations for the most effective solutions for your panel needs. ProcartaPlex Multiplex Immunoassays represent the most flexible Luminex assay system on the market. More than 90% of our ProcartaPlex assays can be combined with one another.

* Analytes only available as part of a panel

ProcartaPlex® AnalytesH

um

an

Mo

use

Rat

NH

P

Can

ine

Porc

ine

4-1BB (CD137) n

4-1BBL (CD137L) n

Albumin* n

Adiponectin n n n

Antithrombin* n

APRIL n

BAFF n n

BLC (CXCL13) n n

BDNF n n

Betacellulin (BTC) n

BTLA (CD272 n

C-peptide n

CD27 n

CD28 n

CD80 (B7-1) n

Calcitonin n

Caspase-3 (total) n

CD30 n

CD30L n

CD40 n

CD40L n n

CD44var (6var) n

CRP n n n

Clusterin* n

CTACK (CCL27) n

CTLA-4 (CD152) n

Cortisol n

Cysistin C* n

D-Dimer n

DR6 n

EDA-1 n

EGF n

ENA-78 (CXCL5) n n

Endoglin (CD105) n

Eotaxin (CCL11) n n n n

Eotaxin-2 (CCL24) n

Eotaxin-3 (CCL26) n

E-selectin (CD62E) n

Factor V* n

Factor VII* n

Factor VIII* n

Factor IX* n

Factor X* n

Factor XI* n

Factor XII* n

Factor XIII* n

Fas (APO) n

Fas-L n

FGF-2 n n

FGF-23 n

Fibrinogen n

Fractalkine (CX3CL1) n

Galectin-3 n

GAPDH n

G-CSF (CSF-3) n n n n

GITRL n

GITR (CD357) n

GM-CSF n n n n

Granzyme B n n

GRO alpha (KC/CXCL1) n n n

GLP-1 n

Ghrelin n

HER-2 n

HGF n

HVEM (CD270) n

Insulin n

ICAM-1 n n n

ProcartaPlex® Analytes

Hu

man

Mo

use

Rat

NH

P

Can

ine

Porc

ine

IFN alpha n n n n

IFN beta n n n

IFN gamma n n n n n n

IFN omega n n

IgA* n n n

IgE* n n

IgG1* n n n

IgG2* n

IgG2a* n n

IgG2b* n n

IgG2c* n n

IgG3* n n

IgG4* n

IgM* n n n

IDO n

IL-1 alpha n n n

IL-1 beta n n n n n

IL-1 RA n n

IL-2 n n n n n

IL-2RA (CD25) n n

IL-3 n n

IL-4 n n n n n n

IL-5 n n n n

IL-6 n n n n n n

IL-7 n n n

IL-7RA (CD127) n

IL-8 (CXCL8) n n n n

IL-9 n n

IL-10 n n n n n n

IL-12/IL-23p40 n n n n n n

IL-12 p70 n n n n

IL-13 n n n n

IL-15 n n

IL-15/IL-15R n

IL-16 n

IL-17A (CTLA-8) n n n n

IL17AF n

IL-17F n n n

IL-18 n n n

IL-19 n

IL-20 n

IL-21 n n

IL-22 n n

IL-23 n n n

IL-25 n

IL-27 n n

IL-29 (IFN lambda 1) n

IL-31 n n

IL-33 n

IL-37 n

IP-10 (CXCL10) n n n n

I-TAC (CXCL11) n n

KIM-1* n

LAG-3 n

LAP (TGF beta 1) n

Leptin n n n

Light n

LIF n n

L-selectin n

MCP-1 (CCL2) n n n n n

MCP-2 (CCL8) n

MCP-3 (CCL7) n n n n

M-CSF n n

MDC (CCL22) n

MIF n n

MIG (CXCL9) n n

MIP-1 alpha (CCL3) n n n n

ProcartaPlex® Analytes

Hu

man

Mo

use

Rat

NH

P

Can

ine

Porc

ine

MIP-1 beta (CCL4) n n n

MIP-2 alpha (CXCL2) n n

MIP-3 alpha (CCL20) n

MMP-1 n

MMP-2 n

MMP-3 n

MMP-7 n

MMP-8 n

MMP-9 n

MMP-12 n

MMP-13 n

Myeloperoxidase (MPO) n

NGAL n n*

NGF beta n n n n

Oncostatin M (OSM) n

Osteopontin n n*

Osteoprotegerin (OPG) n

PAI-1 (Serpin) n n

PDGF-BB n n

PD-1 (CD279) n

PD-L1 (CD274) n

PD-L2 n

PECAM-1 n

PIGF n

Protein C* n

Protein S* n

Prothrombin* n

P-selectin n

PYY n

RBP4 n

RANKL n n n

RANTES (CCL5) n n n n

Resistin (ADSF) n n

SAA n

SAP (Pentraxin 2) n

SCF n n n

SCGF beta n

SDF-1 alpha (CXCL12a) n n

ST2 (IL-33R) n

Survivin (BIRC5) n

TGF alpha n n

TGF beta 1 n n n n n n

Thrombopoietin (TPO) n

TIM-3 (CD366) n

TIMP-1 n n*

TFF-3* n

TNF alpha n n n n n n

TNF beta n n

TNF R1 n

TNF R2 n

Tissue Plasmon Activator n

TRAIL n n

TRAIL-R1 n

TRAIL-R2 n

TSLP n n

Tweak n

VCAM-1 n n n

VEGF-A n n n n n

VEGF-D n n

VEGF-R1 n

VEGF-R2 n

VEGF-R3 n

vWF* n

ZAG n

Multiplex

20

ProcartaPlex® Panels & Bead SetsHuman ProcartaPlex® Panels

Th1/Th2 Cytokine Panel (11 plex)Cat. No. EPX110-10810-901

GM-CSF IL-2 IL-6 IL-18IFN gamma IL-4 IL-12 p70 TNF alphaIL-1 beta IL-5 IL-13

Th9/Th17/Th22/Treg Cytokine Panel (7 plex)Cat. No. EPX070-10817-901

IL-9 IL-17A IL-22 IL-27IL-10 IL-21 IL-23

Cytokine Panel 1C (7 plex)Cat. No. EPX070-10010-901

IFN alpha IL-1RA IL-15 TNF beta (LTA)IL-1 alpha IL-7 IL-31

Chemokine Panel 1 (8 plex)Cat. No. EPX080-10007-901

Eotaxin (CCL11) MIP-1 alpha (CCL3)GRO alpha (CXCL1)

MIP-1 beta (CCL4)

IL-8 (CXCL8) SDF-1 alpha (CXCL12)IP-10 (CXCL10) RANTES (CCL5) MCP-1 (CCL2) RANTES

Growth Factor Panel 1 (11 plex)Cat. No. EPX110-12170-901

BDNF HGF SCFNGF beta LIF VEGF-A

EGF PDGF-BB VEGF-DFGF-2 PlGF

Human Cytokine/Chemokine/Growth Factor ProcartaPlex® PanelsEssential Th1/Th2 Cytokine Panel (6 plex)

Cat. No. EPX060-20831-901IFN gamma IL-5 IL-12 p70

IL-4 IL-6 TNF alpha

Essential Panel 2 (4 plex)Cat. No. EPX040-10008-901

IL-10 beta IL-8 (CXCL8) IL-10 IL-17A (CTLA-8)

Inflammation Panel (20 plex)Cat. No. EPX200-12185-901

E-selectin IL-1 alpha IL-10 MCP-1 (CCL2)GM-CSF IL-1 beta IL-12p70 MIP-1 beta

(CCL4)ICAM-1 IL-4 IL-13 MIP-1 alpha

(CCL3)IFN alpha IL-6 IL-17A (CTLA-8) P-selectin

IFN gamma IL-8 (CXCL8) IP-10 (CXCL10) TNF alpha

Myokine Panel (8 plex)Cat. No. EPX080-12186-901

BDNF IL-6 IL-10 LIFIL-1RA IL-8 (CXCL8) IL-15 TNF alpha

Human Antibody Isotyping ProcartaPlex® PanelAntibody Isotyping Panel (7 plex)*

Cat. No. EPX070-10818-901IgG1 IgG2 IgG3 IgG4IgA IgE IgM -

Human Metabolism/Obesity PanelsMetabolism/Obesity Panel 1 (5 plex)*

Cat. No. EPX050-15805-901RBP4 ICAM-1 NGAL AdiponectinZAG

Metabolism/Obesity Panel 2 (9 plex) *Cat. No. EPX090-15804-901

C-peptide Cortisol FGF-23 GLP-1Ghrelin Insulin PYY LeptinResisitin

*Panel cannot be combined with other ProcartaPlex Panels or Simplex kits.

Human Coagulation ProcartaPlex® PanelsCoagulation Panel 1 (6 plex)*

Cat. No. EPX060-10824-901Factor XI Factor XII Factor XIII

Antithrombin Prothrombin CRP

Coagulation Panel 2 (3 plex)*Cat. No. EPX030-10823-901

Factor V Factor VII Factor VIII

Coagulation Panel 3 (4plex)*Cat. No. EPX040-10825-901

Factor IX Protein S Protein C vWF

Human MMP ProcartaPlex® PanelsMMP-Panel 1 (5 plex)

Cat. No. EPX050-10015-901MMP-1 MMP-8 MMP-12 MMP-13MMP-7

MMP-Panel 2 (3 plex)*Cat. No. EPX030-10829-901

MMP-2 MMP-3 MMP-9

Human Immuno-Oncology ProcartaPlex® PanelsImmuno-Oncology Panel (14 plex)*

Cat. No. EPX140-15803-901BTLA LAG-3 TIM-3 CD27GITR PD1 CD28 CD152 (CTLA4)

HVEM PD-L1 CD80IDO PD-L2 4-1BB (CD137)

Human TNFSF ProcartaPlex® PanelsTNF Ligand Panel (15 plex)*

EPX150-15801-901APRIL EDA-1 NGF beta TNF betaBAFF FasL RANKL TRAIL

CD30L GlTRL TL-1 alpha TweakCD40L Light TNF alpha 4-1BBL (CD137L)

TNF Receptor Panel (12 plex)*EPX120-15802-901

CD30 DR6 TNF-R1 TRAIL-R1CD40 Fas (APO) TNF-R2 TRAIL-R2GITR HVEM 4-1BB (CD137) CD27

Th9/Th17/Th22/Treg & Cytokine Panel 1C (14 plex) Cat. No. EPX140-12174-901

Cytokine 1C & Chemokine Panel 1 (15 plex) Cat. No. EPX150-12176-901

Th9/Th17/Th22/Treg & Chemokine Panel 1 (15 plex) Cat. No. EPX150-12175-901

Th1/Th2 & Cytokine Panel 1C (18 plex)Cat. No. EPX180-12172-901

Th1/Th2/Th9/Th17/Th22/Treg Cytokine Panel (18 plex) Cat. No. EPX180-12165-901

Th1/Th2 & Chemokine Panel 1 (19 plex)Cat. No. EPX190-12173-901

Cytokine Panel 1B (25 plex)Cat. No. EPX250-12166-901

Cytokine & Chemokine Panel 1A (34 plex)Cat. No. EPX340-12167-901

Cytokine/Chemokine/Growth Factor Panel 1 (45 plex) Cat. No. EPX450-12171-901

A

B

C

B C

B D

A B

C D

A C

A D

A B C D

A B C

C DA B E

D

E

Human ProcartaPlex® Panel Combinations

Multiplex

21

ProcartaPlex® Panels & Bead Sets

A

A

B

C

D

B

Mouse ProcartaPlex® PanelsTh1/Th2 Cytokine Panel (11 plex)

Cat. No. EPX110-20820-901GM-CSF IL-4 IL-13

IFN gamma IL-5 IL-18IL-1 beta IL-6 TNF alpha

IL-2 IL-12 p70

Th9/Th17/Th22/Treg Cytokine Panel (6 plex)Cat. No. EPX060-20822-901

IL-9 IL-17A IL-23IL-10 IL-22 IL-27

Cytokine Panel 1B (10 plex)Cat. No. EPX100-26091-901

G-CSF (CSF-3) IL-31 ENA-78 (CXCL5)IFN alpha IL-1 alpha M-CSF (CSF-1)

IL-15 (IL-15R) IL-3IL-28 LIF


Eotaxin (CCL11) MCP-1 (CCL2) MIP-1 beta (CCL4)GRO alpha (CXCL1) MCP-3 (CCL7) MIP-2 (CXCL2)

IP-10 (CXCL10) MIP-1 alpha

(CCL3)

RANTES (CCL5)

Mouse Cytokine/Chemokine/Growth Factor ProcartaPlex® PanelsEssential Th1/Th2 Cytokine Panel 1 (6 plex)

Cat. No. EPX060-20831-901IFN gamma IL-5 IL-12 p70

IL-4 IL-6 TNF alpha

Myokine Panel (5 plex)Cat. No. EPX070-20815-901

IL-10 IL-15R/IL15R IL-6LIF TNF alpha

IFN alpha/IFN beta (2 plex)*Cat. No. EPX020-22187-901

IFN alpha IFN beta

Rat ProcartaPlex® PanelsTh Complete Panel (14 plex)Cat. No. EPX140-30120-901

G-CSF (CSF-3) IL-2 IL-12p70GM-CSF IL-4 IL-13

IFN gamma IL-5 IL-17A (CTLA-8)IL-1 alpha IL-6 TNF alphaIL-1 beta IL-10


Eotaxin (CCL11) MCP-1 (CCL2) MIP-2 (CXCL2)GRO alpha (KC/

CXCL1)MCP-3 (CCL7) Rantes (CCL5)

IP-10 (CXCL10) MIP-1 alpha (CCL3)

Rat Antibody Isotyping ProcartaPlex® PanelAntibody Isotyping Panel (6 plex)*

Cat. No. EPX060-30123-901IgG1 IgG2a IgG2bIgG2c IgA IgM


B D Th9/Th17/Th22/Treg & Chemokine Panel (15 plex)Cat. No. EPX150-26089-901

Th1/Th2/Th9/Th17/Th22/Treg Cytokine Panel (17 plex)Cat. No. EPX170-26087-901

Th1/Th2 & Chemokine Panel I (20 plex)Cat. No. EPX200-26090-901

Cytokine & Chemokine Panel 1 (26 plex)Cat. No. EPX260-26088-901

Cytokine & Chemokine Panel 1A (36 plex)Cat. No. EPX360-26092-901

A B

A D

A B C D

A B D

Mouse ProcartaPlex® Panel Combinations

Mouse Antibody Isotyping ProcartaPlex® PanelsAntibody Isotyping Panel (7 plex)*

Cat. No. EPX070-20815-901IgG1 IgG2a IgG2bIgG3 IgA IgEIgM

Antibody Isotyping Panel 2 (7 plex)*Cat. No. EPX070-20816-901

IgG1 IgG2b IgG2cIgG3 IgA IgEIgM


Rat Kidney Toxicity ProcartaPlex® PanelsKidney Toxicity Panel 1 (5 plex)*

EPX050-30124-901KIM-1 VEGF-A TFF-3

Osteopontin Calbindin

Kidney Toxicity Panel 2 (5 plex)*EPX050-30125-901

Cystatin C TIMP-1 ClusterinAlbumin NGAL

A B Cytokine & Chemokine Panel (22 plex)Cat. No. EPX220-30122-90

Rat ProcartaPlex® Panel Combination

Multiplex

22

Canine ProcartaPlex® PanelCytokine/Chemokine/Growth Factor Panel 1 (11 plex)

Cat. No. EPX110-50511-901IFN gamma IL-8 (CXCL8) SCF

IL-2 IL-10 TNF alphaIL-4 IL-12/IL-23 p40 VEGF-AIL-6 MCP-1 (CCL2)

A

B

C

D

Non Human Primate ProcartaPlex® PanelsTh Cytokine Panel (14 plex)Cat. No. EPX140-40040-901

GM-CSF IL-5 IL-17AIFN gamma IL-6 IL-18

IL-1 beta IL-10 IL-23IL-2 IL-12p70 TNF alphaIL-4 IL-13

Cytokine Panel 1b (6 plex)Cat. No. EPX060-40042-901

CD40L IFN alpha IL-15G-CSF IL-1 RA IL-7

Chemokine Panel (10 plex)Cat. No. EPX100-40041-901

BLC (CXCL13) I-TAC (CXCL11) MIP-1 beta (CCL4)Eotaxin (CCL11) MCP-1 (CCL2) SDF-1 alpha (CXCL12)

IL-8 (CXCL8) MIG (CXCL9)IP-10 (CXCL10) MIP-1 alpha (CCL3)

Growth Factor Panel (7 plex)Cat. No. EPX070-40043-901

BDNF PDGF-BB VEGF-DNGF beta SCF -FGF-basic VEGF-A -

Porcine ProcartaPlex® PanelCytokine Panel (9 plex)

Cat. No. EPX090-60829-901IFN alpha IL-4 IL-10

IFN gamma IL-6 IL-12/IL-23 p40IL-1 beta IL-8 (CXCL8) TNF alpha

Cytokine & Chemokine Panel (30 plex)Cat. No. EPX300-40044-901

Cytokine/Chemokine/Growth Factor Panel (37 plex)Cat. No. EPX370-40045-901

A B C D

A B C

Non Human Primate ProcartaPlex® Combinations

ProcartaPlex® Panels & Bead Sets

Multiplex

1. Hornig M., et al. Distinct plasma immune signatures in me/cfs are present early in the course of illness. Science Advances B(1):e1400121 (2015).2. Horing M., et al. Cytokine network analysis of cerebrospinal fluid in myalgic encephalomyelitis/chronic fatigue syndrome. Molecular Psychiatry 10:1038 (2015).3. DiCarlo J., et al. Cytokine and Chemokine Patterns Across 100 Days after Hematopoietic Stem Cell Transplantation in Children. Biology of Blood and Marrow Transplantation 20(3):361–369 (2014).4. Siebert J. C., et al. The Stanford Data Miner: a novel approach for integrating and exploring heterogeneous immunological data. Journal of Translational Medicine 10:62 (2012).

References

23

High Sensitivity ProcartaPlex Immunoassays High Sensitivity ProcartaPlex Immunoassays offer accurate quantification of key cytokines significantly below the detection limit of conventional Luminex assays.

High Sensitivity ProcartaPlex Immunoassays enable the multiplexed analysis of human and mouse analytes in very low

concentrations in various matrices including serum, plasma, bodily fluids, and cell culture supernatant samples. These high-

sensitivity multiplex assays are ideal when sample input is limited, and protein expression is in the low pg/mL range.

Accurate quantification below conventional limits is achieved through a proprietary signal amplification technology. The signal

amplification has negligible effects on the assay background, thereby increasing the signal-to-noise ratio of the cytokine

standards on the lower end of the standard curve. This also allows for more accurate and reproducible measurement of

cytokines with low expression levels.

All analytes listed above are also available as Simplex Kits. Additional ProcartaPlex® High Sensitivity Basic Kit that includes all non-target specific reagents is needed to perform assay.

Assay specifications and data

Measure low-abundant cytokines with our High Sensitivity ProcartaPlex® Immunoassays. We offer two high-sensitivity panels

for the following human and mouse cytokines. Please visit www.ebioscience.com to find the most current offering of our

available high sensitivity assays.

High Sensitivity ProcartaPlex assays feature

Highest sensitivity detection of cytokine concentrations; in fg/mL range

Inter- and intra-assay coefficients of variation (CV) <10%

Small sample input (25 μL) for serum or plasma samples; ideal for precious and limited samples

Easy-to-use, simple ELISA-like protocol completed in under four hours

Convenient, ready-to-use kits for quantification of up to nine cytokines

Trusted platform based on the principles of a sandwich ELISA and utilizing Luminex xMAP (multi-analyte profiling) technology

High Sensitivity ProcartaPlex® PanelsHuman High Sensitivity Panel (9 plex)

Cat. No. EPXS090-12199-901IFN gamma IL-4 IL-12 p70IL-1 beta IL-6 IL-17A (CTLA-8)

IL-2 IL-10 TNF alpha

Mouse High Sensitivity Panel (5 plex)Cat. No. EPXS050-22199-901

IFN gamma IL-4 TNF alphaIL-2 IL-6

Human High Sensitivity ProcartaPlex® Panels (9 plex)

Cat. No. EPXS090-12199-901

Analyte ULOQ* (pg/mL)

LLOQ** (pg/mL)

Inter-assay CV

Intra-assay CV

IFN gamma 1,250 0.31 <10 <10

IL-1 beta 1,330 0.32 <10 <10

IL-2 1,820 0.44 <10 <10

IL-4 3,530 0.86 <10 <10

IL-6 4,230 1.03 <10 <10

IL-10 750 0.18 <10 <10

IL-12p40 3,090 0.75 <10 <10

IL-17A 1,030 0.25 <10 <10

TNF alpha 1,830 0.45 <10 <10

Mouse High Sensitivity ProcartaPlex® Panels (5 plex)

Cat. No. EPXS050-22199-901

Analyte ULOQ* (pg/mL)

LLOQ** (pg/mL)

Inter-assay CV

Intra-assay CV

IFN gamma 260 0.06 <10 <10

IL-2 490 0.12 <10 <10

IL-4 560 0.14 <10 <10

IL-6 2,850 0.70 <10 <10

TNF alpha 1,390 0.34 <10 <10

* upper limit of quantitation** lower limit of quantitation

Multiplex

24

Platinum ProcartaPlex® Immunoassays

Platinum ProcartaPlex Immunoassays are our most validated bead-based multiplexing assay system. Similar to our Platinum ELISAs, Platinum ProcartaPlex assays have to pass more than 30 qualification criteria to guarantee reproducible high performance of the kits.

Overcome matrix effects

Serum and plasma samples are complex matrixes with many components. This complexity can cause the “matrix effect” which

can impact the readout of many cytokines (low spike and dilution recovery). Affymetrix developed matrix-type specific diluents

to overcome these matrix and multiplex effects, assuring high-performance specifications comparable to those of traditional

ELISA. Spike and dilution recovery in a range between 70–130% are obtainable with Platinum ProcartaPlex assays.

100% combinable

Platinum ProcartaPlex Immunoassays are available in premixed panels and simplex kits. All Platinum ProcartaPlex Immunoassays are combinable with one another, eliminating any limitations on composition and size of your ProcartaPlex panel. You can select between two off the shelf panels or compile your own custom panel.

Platinum ProcartaPlex Assays feature

Matrix-specific diluents: Optimized diluents for the use with serum and plasma samples

High performance: Spike recovery and dilution linearity between 70–130%

Sample friendly: Only 20 μL serum/plasma samples needed

100% combinability: All ProcartaPlex Simplex kits can be combined with one another

All in one: 100% of needed reagents are included in our panels

Dilution and spike recovery were evaluated in a minimum of five individual donor samples per matrix by performing 4-fold serial dilutions, from 1:4 to 1:256. Mean recoveries for each matrix were calculated across the complete portfolio of Platinum ProcartaPlex® assays, with a target specification of 100% +/–30%.

140%

130%

120%

110%

100%

90%

80%

70%

60%

50%

40%

30%

20%

10%

0%

IFN gamma IL-12p70 IL-13 IL-1 beta IL-2 IL-4 IL-6 IL-7 GRO alpha IL-8 PDGFbb

92% 96% 90% 95% 84% 90% 95% 92% 90% 94% 101%

91% 99% 90% 95% 92% 90% 97% 93% 84% 89% 101%

94% 99% 93% 100% 92% 96% 94% 97% 87% 90% 103%

TNF alpha GM-CSF IL-10 IP-10 VEGF-D BDNF LIF IL-16 TSLP IL-20 TNF R II

99% 90% 79% 99% 88% 82% 98% 84% 94% 94% 84%

112% 89% 91% 86% 83% 86% 100% 91% 102% 99% 93%

111% 94% 91% 97% 95% 85% 110% 95% 102% 95% 79%

n Serum n EDTA n Citrat n Serum n EDTA n Citrat

Dilution recovery Spike recovery

Platinum ProcartaPlex® Human Panels

Platinum Human Panel 1 (42 plex)Cat. No. EPXP420-10200-901

BDNF IL-15 IL-7 PECAM-1Eotaxin IL-16 IL-8 P-SelectinGM CSF IL-17A IL-9 RANTES

GRO alpha IL-1 beta IP-10 SCFHGF IL-1RA LIF TNF alpha

IFN alpha IL-2 MCP-2 TNFRII IFN gamma IL-20 MIP-1 alpha tPA IL 1 alpha IL-21 MIP-1 beta TSLP

IL-10 IL-4 OPG VEGFAIL12p70 IL-5 PDGF BB VEGFD

IL13 IL-6

Platinum Human Panel 2 (13 plex)Cat. No EPX130-10100-901

IFN gamma IL-2 IL-8 GM CSF IL12p70 IL-4 IL-10

IL13 IL-5 IL-17A IL-1 beta IL-6 TNF alpha

All analytes listed above are also available as Platinum Simplex Kits. Additional Platinum ProcartaPlex® Basic Kit for serum or plasma samples is needed to perform assay.

Multiplex

25

Affymetrix Services

Fast track to results with Affymetrix Services

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26

ELISA Troubleshooting

Problem Solutions

High Background Improper washing

Insufficient washing or omission of one of the wash steps can lead to high background. Check volume of ELISA wash buffer and make sure all recommended washing steps are performed. Make sure the eBioscience ELISA wash buffer is used and diluted correctly.

Leave the ELISA wash buffer in the wells for at least 15–30 seconds.

Contaminated substrate

Make sure there is no contamination of the substrate before using with metal ions or oxidizing reagents. The substrate solution should not develop color by itself.

Contaminated diluent

Most of the ELISA diluents are based on a protein matrix. When first opening, contamination of the ELISA diluent may occur. Use only sterilized pipettes to pipette ELISA diluent from the bottle.

Substrate exposed to light

Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until it is ready to be dispensed on the plate.

Wrong conjugate dilution

Make sure the conjugate is diluted according to the instruction in the manual. Too high concentration of the conjugate may result in high background.

Wrong incubation times/temperatures

Adhere to the instructions in the ELISA test protocol regarding incubation times and temperature.

High Intra-CV Coating removed by scratching during washing/pipetting

Take care not to scratch the inner surface of the microwells when washing the plate or pipetting standards and samples.

Inhomogeneous samples

Make sure the samples you use for the test are homogeneous. Mix before adding to the ELISA plate.

Edge effect

Avoid incubating plates in areas where environmental conditions vary (e.g., under a vent or window sill). Seal the ELISA plate during incubation and incubate on a rotator set at 400 rpm, if possible.

Do not stack the plates during incubation.

Pipettes not properly calibrated

Make sure the pipettes used are properly calibrated.

Improper washing

Insufficient washing or omission of one of the wash steps can lead to high CV values.

Do not reduce wash volume or the number of wash steps. Leave the ELISA wash buffer in the wells for 15–30 seconds.

ELISA troubleshooting

27


Problem Solutions

Poor Standard Curve Wrong reconstitution volume of lyophilized standard

Make sure the lyophilized powder is reconstituted in the correct volume of the correct reconstitution media. Reconstitution volumes are given in the manual, on the vial label, or both.

Reconstitution time too short

Make sure the reconstitution process is given enough time to completely solubilize the lyophilized powder.

Wrong dilution of concentrated stock standard

Make sure the concentrated stock standard provided is diluted properly according the manual.

Wrong storage of stock material/pre-dilutions

Storage conditions of stock standard solution as well as pre-dilutions are given in the manual. Follow these recommended guidelines.

Improper mixing at standard dilution steps

Prepare serial dilutions of the concentrated standard and mix properly at each step of dilution.

Wrong measurement wave length

Make sure the photometer used is adjusted to the correct wavelength. Please check for the reference wavelength.

Standard from a different lot used

Only use the standard(s) provided in the kit to perform assay. Do not substitute the standard from another kit.

Low OD Values Incubation time with the TMB substrate is too short

The time indicated in the test protocol may vary due to temperature or other assay conditions (temperature, kit age, humidity).

Observe the color development on the plate and use your own judgment to stop the substrate reaction. We recommend adding the ELISA stop solution when the highest standard has developed a dark blue color.

Alternatively you may monitor the color development with the ELISA reader at 620 nm after adding the TMB substrate solution. The substrate reaction should be stopped (with ELISA stop solution) as soon as an OD of 0.9–0.95 is reached.

Wrong reconstitution volume of lyophilized ELISA standard

Reconstitute with the media and volume specified in manual and/or on vial label.

Make sure the reconstitution process is given enough time to allow complete solubilizing of the lyophilized powder.

Shaking

Make sure that assay plate(s) are shaken at the indicated speed on a plate shaker during designated incubation periods. Not shaking the assay plate(s) during these noted periods result in reduced OD values.

28


Problem Solutions

No Color Development Incorrect storage of ELISA reagents

Storage conditions of all ELISA kit components are given in the manual—always follow these guidelines.

Omission of any incubation step

Make sure each step of the ELISA test protocol is performed as described. Addition of the colored reagents (green-/blue-/red-dye) in the Platinum ELISA Kits helps ensure that the assay protocol is followed as designed.

Microwells dried out after washing or during storage

After washing, tap ELISA microwell strips on absorbent pad or paper towel to remove excess ELISA wash buffer. Use the microwell strips immediately after washing or place upside down on a wet, absorbent paper towel for no longer than 15 minutes.

Samples not suitable for the ELISA test

A list of samples suitable for the ELISA test is provided in the manual. Other samples may not be suitable for the ELISA test.

ELISA Samples or Standard in the Overflow

Wrong dilution of sample/standard

Follow the test protocol for dilution of standard and samples.

Wrong diluent used

Only use the diluent delivered with the kit to dilute samples.

Wrong measurement wavelength

Make sure the photometer used is adjusted to the correct wavelength. Please check for the reference wavelength in the manual.

Development time of TMB substrate too long

The time indicated in the test protocol may vary due to temperature or other assay conditions (temperature, kit age, humidity).

Observe the color development on the plate and use your own judgment to stop the substrate reaction. We recommend adding the stop solution when the highest standard has developed a dark blue color.

Alternatively, you may monitor the color development with the ELISA reader at 620 nm after adding the TMB substrate solution. The substrate reaction should be stopped (with stop solution) as soon as an OD of 0.6–0.65 is reached.

Sample not suitable for the test

A list of samples suitable for the test is given in the manual. Any other sample may not be suitable for the test.

Wrong incubation time/temperature

Follow the test protocol regarding incubation time and temperature.

Plate covers were not applied during incubation

Application of plate covers during incubation prevents evaporation of liquid as well as contamination during incubation. Plate covers are supplied with each kit. Use a new plate sealer for each incubation step.

29

Immunoassay productsTargets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*4-1BB (CD137) Human ProcartaPlex® Serum, Plasma: 25 μL; CCS:

50 μL12.21-50000 pg/mL

EPX01A-10289-901

4-1BBL (CD137L) Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

168.95-692000 pg/mL

EPX010-15901-901

Adiponectin Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

4.6 pg/mL 56.15–230,000 pg/mL

EPX01A-12032-901

Platinum 50 µL (1:5,000 prediluted) 0.012 ng/mL 3.10% 4.20% 0.23–15 ng/mL BMS2032/2

Mouse ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

6.3 pg/mL 82.42–337,600 pg/mL

EPX01A-26038-901

NHP ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

2.3 pg/mL EPX01A-42032-901

Annexin V Human Platinum 50 µL 0.33 ng/mL 11% 10% 0.78–50 ng/mL BMS252

Anti-IFN alpha Human Platinum 20 µL 1.4 ng/mL 4.10% 3.30% 3.15–200 ng/mL BMS217

Anti-Annexin V Human Platinum 50 µL 1.2 ng/mL 3.80% 2.00% 6.3–400 ng/mL BMS247

APRIL Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

3.78 pg/mL 7.90% 6.90% 90.70–371,500 pg/mL

EPX01A-12008-901

Platinum 50 µL 0.4 ng/mL 7.10% 8.10% 0.78–50 ng/mL BMS2008

BAFF Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.6 pg/mL 2.9–11,800 pg/mL

EPX01A-12007-901

Instant 50 µL 0.13 ng/mL 9.80% 8.20% 0.31–20 ng/mL BMS2007INST


0.2 pg/mL 2.88-11,800 pg/mL

EPX01A-26096-901

Bcl-2 Human Platinum 20 µL 0.5 ng/mL 12% 8.60% 0.50–32 ng/mL BMS244/3

BDNF Human Platinum ProcartaPlex®

Serum, Plasma: 25 µL 1.94–7,950 pg/mL

EPXP010-12116-901

ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

1.02 pg/mL 7.40% 7.40% 2.44–10,000 pg/mL

EPX01A-12116-901


1.02 pg/mL 7.40% 7.40% 2.44-10,000 pg/mL

EPX01A-42116-901

Betacellulin (BTC) Mouse ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

6.4 pg/mL 30-122,900 pg/mL

EPX01A-26042-901

BLC (CXCL13) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

5 pg/mL 6.3–25,800 pg/mL

EPX01A-12147-901


60 pg/mL 37.89–155,200 pg/mL

EPX01A-42147-901

BTLA (CD272) Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

122.7-500000 pg/mL

EPX01A-12217-901

C1q Human Platinum Serum, Plasma: 10 µL (1:100 prediluted) CCS 50µL

0.08 ng/mL 4.20% 4.70% 0.9–60 ng/mL BMS2099

C3a Human Platinum Plasma, Serum: 10 µL (prediluted 1:500 for Plasma, 1:30 for Serum) CCS: 50 µL

0.07 ng/mL 5.90% 5.90% 0.3–20 ng/mL BMS2089

C5a Human Platinum Serum: 5 µL; Plasma: 10 µL; CCS: 50 µL

0.005 ng/mL 4.00% 3.60% 0.078–5.0 ng/mL BMS2088

Calcitonin Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

1.74 pg/mL 2.23–9,150 pg/mL

EPX01A-12067-901

Caspase-3 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.59 pg/mL 4.88–20,000 pg/mL

EPX01A-12012-901


Caspase-8 Human Platinum 50 µL 0.1 ng/mL 8.50% 6.70% 0.16–10 ng/mL BMS2024

Caspase-9 Human Platinum 50 µL 0.4 ng/mL 9% 6.60% 1.6–100 ng/mL BMS2025

Cathepsin L Human Platinum 50 µL 1.7 ng/mL 6% 6.20% 3.1–50 ng/mL BMS257

CCL2 (MCP-1) Human Ready-SET-Go!® 7 pg/mL 7–1,000 pg/mL 88-7399

Mouse Ready-SET-Go!® 15 pg/mL 15–2,000 pg/mL 88-7391

CCL3 (MIP-1 alpha)

Human Ready-SET-Go!® 16 pg/mL 16–2,000 pg/mL 88-7035

CCL4 (MIP-1 beta) Human Ready-SET-Go!® 4 pg/mL 4–500 pg/mL 88-7034

CCL8 (MCP-2) Human Ready-SET-Go!® 0.16 pg/mL 0.16–10 pg/mL 88-52169-22

CCL21 (SLC) Human Ready-SET-Go!® 40.6 pg/mL 40.6–2,600 pg/ml

88-58214-22

CD23 Human Platinum 100 µL 6.8 U/mL 6.30% 4.00% 12.5–400 U/mL BMS227/2**Also available as CE-marked ELISA Kit

30

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*CD26 Human Platinum 20 µL 7.3 ng/mL 9.10% 4.60% 15.6–500 ng/mL BMS235*

CD27 Human Instant 50 µL (1:25 prediluted) 0.264 U/mL 5.90% 7.90% 0.31–20 U/mL BMS286INST

Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

4.88-20000 pg/mL

EPX01A-10286-901

CD28 Human Platinum 50 µL 0.18 ng/mL 7.20% 5.90% 0.78–50 ng/mL BMS290


488.28-2000000 pg/mL

EPX01A-10290-901

CD30 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

9.89–40,500 pg/mL

EPX01A-10240-901

CD30 Human Platinum 25 µL 0.3 ng/mL 5.60% 4.10% 1.6–100 ng/mL BMS240*

CD31 Human Instant 25 µL 0.33 ng/mL 12.90% 9.20% 1.6–100 ng/mL BMS240INST*

CD40 Human Platinum 50 µL 1.3 pg/mL 7.00% 5.50% 7.8–500 pg/mL BMS265


2.98-12200 pg/mL

EPX01A-10265-901

CD40L Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

1 pg/mL 5.7–23,400 pg/mL

EPX01A-10239-901


6 pg/mL 5.3–21,800 pg/mL

EPX01A-40239-901

Human Platinum 20 µL 0.01 ng/mL 6.60% 5.50% 0.8–5 ng/mL BMS293*/BMS239*

Human Instant 15 µL 0.062 ng/mL 14.20% 6.80% 0.16–10 ng/mL BMS239INST*

Mouse Platinum 50 µL 0.14 ng/mL 11.10% 6.50% 0.31–20 ng/mL BMS6010

CD44std Human Platinum 20 µL (1:60 prediluted) 0.02 ng/mL 4.10% 4.80% 0.13–4 ng/mL BMS209/2*

Human Instant 20 µL (1:60 prediluted) 0.016 ng/mL 4.10% 9.50% 62.5–4,000 pg/mL

BMS209INST

CD44var (v5) Human Platinum 20 µL (1:6 prediluted) 0.14 ng/mL 5.80% 3.60% 0.31–10 ng/mL BMS220

Human Platinum 20 µL (1:20 prediluted) 0.05 ng/mL 4.20% 3.00% 0.32–10 ng/mL BMS210

Human Instant 20 µL 0.01 ng/mL 7.10% 7.20% 0.16–0 ng/mL BMS220INST

CD44var (v6) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

1.13 pg/mL 5.90% 5.80% 24.4–100,000 pg/mL

EPX01A-10210-901

Human Instant 20 µL (1:20 prediluted) 0.06 ng/mL 10.00% 10.00% 0.16–10 ng/mL BMS210INST

CD80 Human Instant 50 µL 0.1 ng/mL 9.20% 7.20% 0.31–20 ng/mL BMS291INST

CD80 (B7-1) Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

30.52-125000 pg/mL

EPX01A-10291-901

CD93 Human Ready-SET-Go!® 20 µL 3.3 ng/mL 3.3–400 ng/mL 88-50340

CD134 (OX40) Human Platinum 25 µL 1.8 pg/mL 12.00% 8.00% 78–5,000 pg/mL BMS296

CD137 (s4-1BB) Human Platinum 50 µL 0.2 ng/mL 9.90% 7.10% 0.78–50 ng/mL BMS289

CD152 (CTLA-4) Human Platinum 10 µL 0.13 ng/mL 10.40% 4.80% 0.16–10 ng/mL BMS276


12.21-50000 pg/mL

EPX01A-10276-901

CD276 Human Ready-SET-Go!® 0.16 ng/mL 0.16–10 ng/mL 88-50370-22

Cortisol Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

4882.8-20000000 pg/m

EPX010-12190-901

C-peptide Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

48.83-200000 pg/mL

EPX010-12191-901

CRP Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.29 pg/mL 8.60% 6.40% 4–18,000 pg/mL EPX01A-10288-901

Ready-SET-Go!® 150 pg/mL 0.15–10 ng/mL 88-7502-28

Instant 100 µL (1:500 prediluted) 3 pg/mL 13.10% 6.90% 78–5,000 pg/mL BMS288INST*


0.4 pg/mL 40.62–166,400 pg/mL

EPX01A-26045-901

Rat ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.1 pg/mL 2.44–10,000 pg/mL

EPX01A-36047-901

Ready-SET-Go!® 2 ng/mL 2–133 ng/mL 88-7501-28

CTACK (CCL27) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

4.3 pg/mL 7.23–29,600 pg/mL

EPX01A-12188-901

Cu (ZnSOD) Human Platinum 10 µL (1:20 prediluted) 0.04 ng/mL 5.80% 5.10% 0.8–5 ng/mL BMS222*

Cytochrome c Human Platinum 100 µL (prediluted) 0.05 ng/mL 4% 6% 0.8–5 ng/mL BMS263

D-Dimer Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

132 pg/mL 317.99–130,2500 pg/mL

EPX01A-12149-901

DR6 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

17.41–71,300 pg/mL

EPX01A-15904-901

EDA-1 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

12.55–51,400 pg/mL

EPX01A-15903-901

*Also available as CE-marked ELISA Kit

31

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*EGF Human ProcartaPlex® Serum, Plasma: 25 µL; CCS:

50 µL0.85 pg/mL 4.90% 6.00% 2.93–12,000

pg/mLEPX01A-12070-901

Instant 50 µL 0.26 pg/mL 4.10% 3.50% 3.91–250 pg/mL BMS2070INST

Platinum 50 µL 3.6 pg/mL 9.40% 5.60% 31.3–2,000 pg/mL

BMS6008

ENA-78 (CXCL5) Mouse ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

5.67 pg/mL 6.60% 6.80% 11.1–45,400 pg/mL

EPX01A-26043-901

Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.2 pg/mL 8.06–33,000 pg/mL

EPX01A-12119-901

Endoglin (CD105) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.09 pg/ml 3.70% 6.10% 0.61–2,500 pg/mL

EPX01A-12105-901

Instant 50 µL (1:50 prediluted) 2.6 pg/mL 3.30% 4.60% 15.6–1,000 pg/mL

BMS2105INST

Eotaxin (CCL11) Human Platinum ProcartaPlex®


EPXP010-12120-901


0.01 pg/mL 10.20% 9.80% 0.5–2,000 pg/mL EPX01A-26008-901


1.1 pg/mL 11.20% 6.80% 1.9–7,700 pg/mL EPX01A-36008-901


"1.4 pg/mL 7.56–31,000 pg/mL

EPX01A-42120-901


1.4 pg/mL 5.30% 6.00% 0.61–2,500 pg/mL

EPX01A-12120-901

Eotaxin-2 (CCL24) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

2.9 pg/mL 3.42–14,000 pg/mL

EPX01A-12150-901

Eotaxin-3 (CCL26) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.01 pg/mL 0.3–1,400 pg/mL EPX01A-12140-901

Erythropoietin (EPO)

Human Platinum 50 µL 0.17 mIU/mL 8.50% 7.80% 1.6–100 mIU/mL BMS2035*

E-selectin (CD62E) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

292.97–1,200,000 pg/mL

EPX01A-10205-901

Platinum 20 µL 0.3 ng/mL 6.00% 5.40% 0.8–50 ng/mL BMS205*

Instant 20 µL 0.33 ng/mL 7.50% 6.90% 1.56–50 ng/mL BMS205INST*

Monkey Platinum 20 µL 0.4 U/mL 10% 5% 1.9–60 U/mL BMS649

Instant 20 µL 0.6 U/mL 15% 5% 1.88–60 U/mL BMS649INST

Factor X Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

EPX01A-12152-901

Fas (APO) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

26.61 pg/mL 61.035–250,000 pg/mL

EPX01A-10245-901

Fas-L Human Platinum 50 µL 0.07 ng/mL 7.00% 6.10% 0.16–10 ng/mL BMS260/2*


0.7 pg/mL 2.2–9,000 pg/mL EPX01A-10260-901

Fc epsilon RI Human Platinum 0.14 ng/mL 6.10% 6.80% 0.078–5.00 ng/mL

BMS2101

FGF-2 NHP ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

2.47 pg/mL 5.10% 8.20% 4.9–20,000 pg/mL

EPX01A-42074-901


2.47 pg/mL 5.10% 8.20% 4.9–20,000 pg/mL

EPX01A-12074-901

FGF-23 Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

48.83-200000 pg/mL

EPX010-12204-901

Fibrinogen Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

350 pg/mL 1,220.7-5,000,000 pg/mL

EPX01A-12153-901

Fibronectin Human Platinum 50 µL (1:10,000 prediluted) 0.1 ng/mL 6.70% 5.30% 0.31–20 ng/mL BMS2028

Fractalkine (CX3CL1)


0.5 pg/mL 2.08–8,500 pg/mL

EPX01A-12121-901

Galectin-3 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

213.9 pg/mL 439.45–1,800,000 pg/mL

EPX01A-10279-901

Platinum 50 µL 0.29 ng/mL 5.40% 7.50% 0.47–30.0 ng/mL BMS279/4*

GAPDH Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

120 pg/mL 244.14–1,000,000 pg/mL

EPX01A-12154-901


32

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*G-CSF (CSF-3) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS:

50 µL"6.4 pg/mL 16.46–67,400

pg/mLEPX01A-12001-901

Instant 25 µL 11 pg/mL 7.70% 8.60% 39.1–2,500 pg/mL

BMS2001INST*


0.19 pg/mL 4.40% 6.20% 1.4–5,850 pg/mL EPX01A-26034-901


0.3 pg/mL 1.70% 5.10% 3.7–15,000 pg/mL

EPX01A-36034-901


"3.8 pg/mL 4.44–18,200 pg/mL

EPX01A-42001-901

GITR (CD357) Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

24.41-100000 pg/mL

EPX01A-12210-901

GITRL Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

12.41 pg/mL 8.90% 8.90% 54.74–224,200 pg/mL

EPX01A-15902-901

GM-CSF Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

1.2 pg/mL 4.20% 4.20% 17.09–70,000 pg/mL

EPX01A-10283-901

Platinum ProcartaPlex®


EPXP010-10283-901

Ready-SET-Go!® 6 pg/mL 6–750 pg/mL 88- 8337

Platinum 50 µL 2.9 pg/mL 4.40% 6% 7.8–500 pg/mL BMS283

Instant 50 µL 0.6 pg/mL 5.90% 13.50% 7.8–500 pg/mL BMS283INST*

Mouse Platinum 50 µL 2.0 pg/mL 10.00% 5.00% 3.9–250 pg/mL BMS612

Ready-SET-Go!® 4 pg/mL 4–500 pg/mL 88-7334


0.19 pg/mL 4.00% 7.30% 2.4–10,000 pg/mL

EPX01A-20612-901

Rat Platinum 50 µL 3.5 pg/mL 10.00% 5.00% 7.8–500 pg/mL BMS633


1.3 pg/mL 5.90% 7.30% 9.8–40,000 pg/mL

EPX01A-30612-901


1.2 pg/mL 6.37–26,100 pg/mL

EPX01A-40283-901

Granzyme B Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.4 pg/mL 117.4–120,200 pg/mL

EPX01A-12027-901

Platinum 50 µL 0.2 pg/mL 10.40% 8.50% 0.66–480 pg/mL BMS2027




0.6 pg/mL 117.4–120,200 pg/mL

EPX01A-42027-901

Ghrelin Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

195.31-800000 pg/mL

EPX010-12192-901

GLP-1 Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

9.76-40000 pg/mL

EPX010-12194-901

GRO alpha (KC/CXCL1)


2.8 pg/mL 5.00% 5.10% 2.44–10,000 pg/mL

EPX01A-12122-901



EPXP010-12122-901


0.05 pg/mL 6.10% 7.30% 1.7–7,000 pg/mL EPX01A-26031-901


1.4 pg/mL 6.90% 7.10% 11.4–46,500 pg/mL

EPX01A-36031-901

HER-2 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.8 pg/mL 10.81–44,300 pg/mL

EPX01A-10207-901

Platinum 100 µL (1:20 prediluted) 0.06 ng/mL 5.80% 1.90% 0.16–10 ng/mL BMS207*

Instant 100 µL (1:20 prediluted) 0.06 ng/mL 7.20% 7.60% 0.16–10 ng/mL BMS207INST*

HGF Human Platinum ProcartaPlex®


EPXP010-12069-901


3.1 pg/mL 2.70% 7.90% 8.79–36,000 pg/mL

EPX01A-12069-901

Instant 50 µL 5.5 pg/mL 8.30% 7.60% 63–4,000 pg/mL BMS2069INST

HSP27 Human Platinum 10 µL 10.9 pg/mL 4.30% 5.80% 15.6–1,000 pg/mL

BMS2086

HSP70 Human Platinum 50 µL 0.052 ng/mL 4.50% 5.30% 0.156–10 ng/mL BMS2087*Also available as CE-marked ELISA Kit

33

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*HSP90 alpha Human Platinum 100 µL (1:25 prediluted) 0.03 ng/mL 10.00% 8.60% 0.156–10 ng/mL BMS2090

HVEM (CD270) Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

24.41-100000 pg/mL

EPX01A-12218-901

ICAM-1 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

76.3 pg/mL 151.85–622,000 pg/mL

EPX01A-10201-901


Instant 10 µL 2.17 ng/mL 12.90% 9.50% 1.56–100 ng/mL BMS201INST*


0.2 pg/mL 3.98–16,300 pg/mL

EPX01A-36048-901

Monkey Platinum 10 µL 1.1 U/mL 10% 5% 6.3–100 U/mL BMS648



4.4 pg/mL 83.23–340,900 pg/mL

EPX01A-40201-901

ICAM-2 Human Platinum 100 µL (1:20 prediluted) 0.3 U/mL 7.00% 6.70% 0.75–24 U/mL BMS236



IDO Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

4.88-20000 pg/mL

EPX01A-12213-901

IFN alpha Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.2 pg/mL 3.60% 5.20% 0.61-2,500 pg/mL

EPX01A-10216-901



EPXP010-10216-901


Platinum 20 µL 3.2 pg/mL 7.20% 4% 7.8–500 pg/mL BMS216*

Instant 20 µL 3.3 pg/mL 7.40% 6% 39–2,500 pg/mL BMS216INST*


3.03 pg/mL 5.10% 4.10% 5.6–22,800 pg/mL

EPX01A-26027-901

Ready-SET-Go!® 0.7 pg/mL 0.7–100 pg/mL 88-8314


BMS6027

Porcine ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.008 pg/mL 0.8–3,300 pg/mL EPX01A-66046-901


0.2 pg/mL 1.41–5,800 pg/mL

EPX01A-40216-901

IFN beta Mouse ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.3 pg/mL 3.9–16,000 pg/mL

EPX01A-26044-901


0.68 pg/mL 8.54–35,000 pg/mL

EPX01A-12088-901


34

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*IFN gamma Human Platinum

ProcartaPlex®


EPXP010-10228-901

ProcartaPlex® High Sensitivity

Serum, Plasma: 25 µL; CCS: 50 µL

0.075 pg/mL 6.04% 6.79% 0.31–1,250 pg/mL

EPXS010-10228-901


0.2 pg/mL 5.50% 3.60% 12.21–50,000 pg/mL

EPX01A-10228-901

Platinum 50 µL 0.99 pg/mL 5.70% 4.50% 1.6–100 pg/mL BMS228*

High Sensitivity

50 µL 0.06 pg/mL 7.10% 6.80% 0.16–10 pg/mL BMS228HS


0.09 pg/mL 5.40% 9.30% 1.2–5,000 pg/mL EPX01A-20606-901



0.024 pg/mL 0.06–260 pg/mL EPXS010-20606-901

Platinum 50 µL 5.3 pg/mL 10.00% 5.00% 15.6 - 1,000 pg/mL

BMS606

Platinum (extra sensitive)

20 µL 0.09 pg/mL 2.70% 3.50% 0.63–40 pg/mL BMS609

Instant 50 µL 4 pg/mL 10% 5% 15.6–1,000 pg/mL

BMS606INST


0.6 pg/mL 3.90% 5.40% 9.8–40,000 pg/mL

EPX01A-30621-901

Platinum 50 µL 9.9 pg/mL 10.00% 5% 31.3–2,000 pg/mL

BMS621


5.6 pg/mL 1.02–4,200 pg/mL

EPX01A-40228-901

Canine ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

4.1 pg/mL 6.37–26,100 pg/mL

EPX01A-50501-901


1.245 pg/mL 4.98–20,400 pg/mL

EPX01A-66047-901

IFN omega Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.6 pg/mL 5.49–22,500 pg/mL

EPX01A-10233-901


IgA Human Ready-SET-Go!® 20 µL (1:10,000 prediluted) 1.6 ng/mL 1.6–100 ng/mL 88-50600

Platinum 20 µL (1:10,000 prediluted) 0.03 ng/mL 4.80% 3.90% 1.6–100 ng/mL BMS2096*

Mouse Ready-SET-Go!® 10 µL (1:10,000 prediluted) 0.39 ng/mL 0.39–25 ng/mL 88-50450

Rat Ready-SET-Go!® 50 µL (1:1,000 prediluted) 16 ng/mL 16–1,000 ng/mL 88-50480

IgE Human Ready-SET-Go!® 10 µL 7.8 ng/mL 7.8–500 ng/mL 88-50610


Mouse Ready-SET-Go!® 50 µL (1:10 prediluted) 4 ng/mL 4–250 ng/mL 88-50610


IgG total Human Ready-SET-Go!® 100 µL (1:500,000 prediluted ) 1.6 ng/mL 1.6–100 ng/mL 88-50550

Platinum 100 µL (1:500,000 prediluted) 0.24 ng/mL 2.60% 5.30% 1.6–100 ng/mL BMS2091


Rat Ready-SET-Go!® 25 µL (1:106 prediluted) 1.6 ng/mL 1.6–100 ng/mL 88-50400

IgG1 Human Ready-SET-Go!® 50 µL (1:1,000 prediluted) 0.16 ng/mL 0.16–10 ng/mL 88-50490

Platinum 50 µL (1:10,000 prediluted) 0.32 ng/mL 3.80% 2.70% 15.6–1,000 ng/mL

BMS2092


Rat Ready-SET-Go!® 20 µL (1:10,000 prediluted) 6.25 ng/mL 6.25–400 ng/mL 88-50500



IgG2a Mouse Ready-SET-Go!® 50 µL (1:10,000 prediluted) 3.9 ng/mL 3.90–250 ng/mL 88-50420


IgG2b Mouse Ready-SET-Go!® 50 µL (1:10,000 prediluted) 3.9 ng/mL 3.90–250 ng/mL 88-50430

Rat Ready-SET-Go!® 10 µL 2 ng/mL 2–125 ng/mL 88-50520

IgG2c Mouse Ready-SET-Go!® 20 µL 0.39 ng/mL 0.39–25 ng/mL 88-50670

Rat Ready-SET-Go!® 10 µL 4 ng/mL 4–250 ng/mL 88-50530


Platinum 25 µL (1:10,000 prediluted) 0.21 ng/mL 3% 5.60% 4.7–300 ng/mL BMS2094

Mouse Ready-SET-Go!® 50 µL (1:10,000 prediluted) 1.875 ng/mL 1.875–125 ng/mL 88-50440*Also available as CE-marked ELISA Kit

35

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*IgG4 Human Ready-SET-Go!® 10 µL (1:100 prediluted) 46.9 ng/mL 46.9–3,000 ng/

mL 88-50590


IgM Human Ready-SET-Go!® 10 µL (1:10,000 prediluted) 15.6 ng/mL 15.6–1,000 ng/mL

88-50620

Platinum 50 µL (1:10,000 prediluted) 1 ng/mL 3.20% 5.10% 15.6–1,000 ng/mL

BMS2098*



Platinum 50 µL 1.1 pg/mL 10% 5.40% 1.6–100 pg/mL BMS243/2*


Platinum 50 µL 4 pg/mL 10% 5% 7.8–500 pg/mL BMS611

Platinum 50 µL 4 pg/mL 10% 5% 15.6–1,000 pg/mL

BMS627

IL- 2R (CD25) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

14 pg/mL 122.07–500,000 pg/mL

EPX01A-10212-901


0.3 pg/mL 5.2–21,300 pg/mL

EPX01A-20212-901

IL-1 alpha Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.1 pg/mL 2.90% 3.30% 0.61–2,500 pg/mL

EPX01A-10243-901



EPXP010-10243-901


0.32 pg/mL 3.80% 5.90% 3.5–14,300 pg/mL

EPX01A-20611-901


3.5 pg/mL 3.10% 4.50% 12.2–50,000 pg/mL

EPX01A-30611-901

IL-1 beta Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.2 pg/mL 3.90% 5.60% 2.44–10,000 pg/mL

EPX01A-10224-901



EPXP010-10224-901



0.145 pg/mL 6.27% 7.69% 0.32–1,330 pg/mL

EPXS010-10224-901


Platinum 50 µL 0.3 pg/mL 8.60% 5.10% 3.9–250 pg/mL BMS224/2*


High Sensitivity



0.14 pg/mL 4.60% 8.30% 1.2–5,000 pg/mL EPX01A-26002-901

Ready-SET-Go!® 25 pg/mL 25–3,000 pg/mL 88-8014


Instant 50 µL 3 pg/mL 5% 7.30% 3.9–250 pg/mL BMS6002INST

Rat Ready-SET-Go!® 39 pg/mL 39–5,000 pg/mL 88-6010


BMS630


1.1 pg/mL 4.60% 4.70% 15.9–65,000 pg/mL

EPX01A-36002-901


0.2 pg/mL 0.95–3,900 pg/mL

EPX01A-40224-901


0.092 pg/mL 3.73–15,300 pg/mL

EPX01A-66048-901

Monkey Platinum 50 µL 0.7 U/mL 10% 10% 6.3–400 U/mL BMS645*Also available as CE-marked ELISA Kit

36

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*IL-1RA Human Platinum

ProcartaPlex®


EPXP010-12080-901


17.8 pg/mL 6.10% 7.00% 34.18–140,000 pg/mL

EPX01A-12080-901

Platinum 50 µL 30 pg/mL 7.40% 7.20% 109–7,000 pg/mL

BMS2080


Platinum 50 µL 9.1 pg/mL 5% 7% 18.8–1,200 pg/mL

BMS221/2*

Instant 50 µL 2.3 pg/mL 7.40% 5.80% 18.8–1,200 pg/mL

BMS221INST*

High Sensitivity


Mouse Ready-SET-Go!® 2 pg/mL 2–200 pg/mL 88-7025


BMS601

High Sensitivity


Rat Platinum 50 µL 11 pg/mL 10% 5% 62.5–2,000 pg/mL

BMS634


17.8 pg/mL 114.8–470,200 pg/mL

EPX01A-42080-901

Monkey Platinum 50 µL 9.1 pg/mL 5% 7% 18.8–1,200 pg/mL

BMS643/2

IL-2 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.8 pg/mL 5.10% 6.20% 4.88–20,000 pg/mL

EPX01A-10221-901



EPXP010-10221-901



0.199 pg/mL 4.59% 6.65% 0.44–1,820 pg/mL

EPXS010-10221-901

Mouse ProcartaPlex® High Sensitivity


0.168 pg/mL 0.12–490 pg/mL EPXS010-20601-901


0.10 pg/mL 4.10% 6.40% 1.2–5,000 pg/mL EPX01A-20601-901


1.9 pg/mL 3.40% 5.20% 4.9–20,000 pg/mL

EPX01A-36052-901


0.05 pg/mL 0.46–1,900 pg/mL

EPX01A-40221-901


1 pg/mL "7.32–30,000 pg/mL

EPX01A-50504-901

IL-2R Human Platinum 50 µL 0.27 ng/mL 9.80% 7.20% 0.31–20 ng/mL BMS212/2*

Instant 10 µL 0.21 ng/mL 10% 7.70% 0.31–20 ng/mL BMS212INST*


10.1 pg/mL 25.76–105,500 pg/mL

EPX01A-12123-901


0.11 pg/mL 4.50% 5.50% 0.4–1,700 pg/mL EPX01A-26035-901


37

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*IL-4 Human ProcartaPlex®

High Sensitivity


0.237 pg/mL 9.10% 8.40% 0.86–3,530 pg/mL

EPXS010-10225-901



EPXP010-10225-901


1.5 pg/mL 8.30% 9.90% 12.21–50,000 pg/mL

EPX01A-10225-901




High Sensitivity

50 µL 0.1 pg/mL 11% 8.80% 0.25–16 pg/mL BMS225HS


0.03 pg/mL 3.50% 6.60% 1.2–5,000 pg/mL EPX01A-20613-901



0.055 pg/mL 0.14–560 pg/mL EPXS010-20613-901


Platinum 50 µL 2 pg/mL 10% 5% 3.9–250 pg/mL BMS613

Instant 50 µL 0.6 pg/mL 11.10% 6.60% 3.9–250 pg/mL BMS613INST

High Sensitivity



0.1 pg/mL 1.50% 3.00% 1.2–5,000 pg/mL EPX01A-30613-901

Platinum 50 µL 0.2 pg/mL 10% 5% 1.6–100 pg/mL BMS628


3.8 pg/mL 8.76–35,900 pg/mL

EPX01A-40225-901


0.072 pg/mL 1.44–5,900 pg/mL

EPX01A-66050-901


0.3 pg/mL 6.30% 4.40% 7.32–30,000 pg/mL

EPX01A-10278-901



EPXP010-10278-901





0.32 pg/mL 6.00% 8.00% 2.4–10,000 pg/mL

EPX01A-20610-901


Platinum 50 µL 3.3 pg/mL 10% 5% 7.8–500 pg/mL BMS610


0.3 pg/mL 5.10% 6.70% 2.4–10,000 pg/mL

EPX01A-30610-901


0.3 pg/mL 10.35–42,400 pg/mL

EPX01A-40278-901


38

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*IL-6 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS:

50 µL0.4 pg/mL 4.50% 6.20% 9.77–40,000

pg/mLEPX01A-10213-901



EPXP010-10213-901



0.038 pg/mL 9.53% 8.52% 1.03–4,230 pg/mL

EPXS010-10213-901



Instant 50 µL 0.92 pg/mL 7% 6.20% 3.1–200 pg/mL BMS213INST*

High Sensitivity

50 µL 0.03 pg/mL 6% 4.90% 0.8–5 pg/mL BMS213HS


0.21 pg/mL 6.90% 9.10% 4.9–20,000 pg/mL

EPX01A-20603-901



0.066 pg/mL 0.70–2,850 pg/mL

EPXS010-20603-901


Platinum 50 µL 6.5 pg/mL 8.90% 5% 31.3–2,000 pg/mL

BMS603/2

High Sensitivity



1.9 pg/mL 3.20% 4.70% 4.9–20,000 pg/mL

EPX01A-36053-901

Platinum 50 µL 12 pg/mL 10% 5% 31–2,000 pg/mL BMS625

Monkey Platinum 50 µL 1.3 U/mL 10% 5% 1.9–120 U/mL BMS641/2



0.4 pg/mL 4.028–16,500 pg/mL

EPX01A-40213-901


1 pg/mL 12.13–49,700 pg/mL

EPX01A-50505-901


0.428 pg/mL 7.6–31,400 pg/mL

EPX01A-66051-901

IL-6R (CD126) Human Platinum 20 µL (1:50 prediluted) 0.01 ng/mL 2.20% 1.70% 0.8–5 ng/mL BMS214

Instant 20 µL (1:50 prediluted) 0.01 ng/mL 8.40% 6.80% 78–5,000 pg/mL BMS214INST*


0.2 pg/mL 5.60% 4.80% 0.61–2,500 pg/mL

EPX01A-10237-901



EPXP010-10237-901


0.3 pg/mL 7.59–31,100 pg/mL

EPX01A-26094-901


0.2 pg/mL 0.89–3,650 pg/mL

EPX01A-40237-901

IL-7R alpha Mouse ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

3.2 pg/mL 8–32,800 pg/mL EPX01A-26093-901

IL-8 Human Ready-SET-Go!® 2 pg/mL 2–250 pg/mL 88-8086

Platinum 50 µL 2 pg/mL 8.70% 6.30% 15.6–1,000 pg/mL

BMS204/3*


BMS204/3INST*

Monkey Platinum 50 µL 3.0 U/mL 8.70% 6.30% 19.5–1,250 U/mL BMS640/3

Instant 50 µL 1.5 U/mL 10% 5% 19.5–1,250 U/mL BMS640/3INST

IL-8 (CXCL8) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

1.2 pg/mL 4.60% 8.50% 2.44–10,000 pg/mL

EPX01A-10204-901



EPXP010-10204-901


1.2 pg/mL 3.19–13,100 pg/mL

EPX01A-40204-901


1.43 pg/mL 15.77–64,600 pg/mL

EPX01A-66052-901


6.9 pg/mL 10.08–41,300 pg/mL

EPX01A-50506-901


39


50 µL0.5 pg/mL 2.30% 2.60% 9.77–40,000

pg/mLEPX01A-12081-901



EPXP010-12081-901




0.28 pg/mL 8.90% 9.70% 18.3–75,000 pg/mL

EPX01A-26041-901



0.1 pg/mL 2.30% 3.10% 2.44–10,000 pg/mL

EPX01A-10215-901



EPXP010-10215-901



0.038 pg/mL 6.08% 7.58% 0.18–750 pg/mL EPXS010-10215-901


Platinum 50 µL 1 pg/mL 5.60% 3.20% 3.15–200 pg/mL BMS215/2*


High Sensitivity



0.69 pg/mL 4.50% 6.90% 2.4–10,000 pg/mL

EPX01A-20614-901



BMS614/2


BMS614INST


1.6 pg/mL 3.10% 5.00% 9.8–40,000 pg/mL

EPX01A-36049-901


BMS629




0.1 pg/mL 0.75–3,100 pg/mL

EPX01A-40642-901


0.7 pg/mL 8.59–35,200 pg/mL

EPX01A-50502-901


1.25 pg/mL 3.75–15,400 pg/mL

EPX01A-66049-901

IL-12/IL-23 p40 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.2 pg/mL 1.81–7,400 pg/mL

EPX01A-12090-901

Platinum 50 µL <10 pg/mL 6.40% 4.70% 31.3–2,000 pg/mL

BMS2013



BMS616


0.5 pg/mL 4–16,200 pg/mL EPX01A-26033-901


0.6 pg/mL 4.05–16,600 pg/mL

EPX01A-36050-901


0.2 pg/mL 1.81–7,400 pg/mL

EPX01A-42090-901


4.8 pg/mL "6.62–27,100 pg/mL

EPX01A-50503-901


1.46 pg/mL 45.14–184,900 pg/mL

EPX01A-62090-901


40

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*IL-12 p70 Human Platinum

ProcartaPlex®


EPXP010-10238-901


0.04 pg/mL 5.00% 5.60% 6.84–28,000 pg/mL

EPX01A-10238-901



0.044 pg/mL 7.40% 7.16% 0.75–3,090 pg/mL

EPXS010-10238-901


Platinum 50 µL 2.1 pg/mL 4.80% 3% 3.1–200 pg/mL BMS238*

Instant 50 µL 0.99 pg/mL 3.13–200 pg/mL BMS231INST

High Sensitivity



0.21 pg/mL 6.90% 9.70% 2.4–10,000 pg/mL

EPX01A-26004-901


Platinum 50 µL 10.0 pg/mL 11% 8.30% 15.6–1,000 pg/mL

BMS6004

Monkey Platinum 50 µL 2.6 U/mL 4.80% 5% 3.9–250 U/mL BMS646


5.9 pg/mL 4.90% 9.70% 9.8–40,000 pg/mL

EPX01A-36004-901


0.04 pg/mL 1.5–6,150 pg/mL EPX01A-40238-901

IL-13 Human Platinum ProcartaPlex®


EPXP010-10231-901


0.1 pg/mL 2.80% 2.70% 2.44–10,000 pg/mL

EPX01A-10231-901


Platinum 50 µL 2.8 pg/mL 5% 4.60% 7.8–500 pg/mL BMS231/3

Instant 50 µL 0.99 pg/mL 3.13–200 pg/mL BMS231INST


Platinum 50 µL 2.8 pg/mL 5% 4.60% 7.8–500 pg/mL BMS6015


0.16 pg/mL 6.00% 8.60% 2.4–10,000 pg/mL

EPX01A-26015-901


0.3 pg/mL 4.40% 8.90% 4.9–20,000 pg/mL

EPX01A-36015-901


0.1 pg/mL 1.57–6,450 pg/mL

EPX01A-40231-901



EPXP010-12089-901


1.1 pg/mL 3.70% 7.60% 3.05–12,500 pg/mL

EPX01A-12089-901



1.1 pg/mL 3.54–14,500 pg/mL

EPX01A-42089-901

IL-15/IL-15R Mouse ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.42 pg/mL 4.60% 6.10% 2.0–8,050 pg/mL EPX01A-26023-901


Platinum 50 µL 2 pg/mL 6.30% 5.30% 6.3–400 pg/mL BMS6023



EPXP010-12162-901


BMS2106


4.7 pg/mL 15.45–63,300 pg/mL

EPX01A-12162-901

IL-17A Human Ready-SET-Go!® 4 pg/mL 4–500 pg/mL 88-7176


High Sensitivity




Rat Ready-SET-Go!® 2 pg/mL 2–200 pg/mL 88-7170

Platinum 50 µL 1 pg/mL 7.60% 8.50% 1.6–100 pg/mL BMS635*Also available as CE-marked ELISA Kit

41

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*IL-17A (CTLA-8) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS:

50 µL0.1 pg/mL 6.00% 6.50% 2.44–10,000

pg/mLEPX01A-12017-901



0.016 pg/mL 6.50% 8.50% 0.25–1,030 pg/mL

EPXS010-12017-901



EPXP010-12017-901


0.08 pg/mL 4.20% 6.90% 1.2–5,000 pg/mL EPX01A-26001-901


0.2 pg/mL 2.80% 3.40% 3.7–15,000 pg/mL

EPX01A-30635-901


3.4 pg/mL 6.98–28,600 pg/mL

EPX01A-42017-901

IL-17AF Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

2.55 pg/mL 6.1–25,000 pg/mL

EPX01A-12082-901



BMS2082



IL-17E (IL-25) Mouse Ready-SET-Go!® 16 pg/mL 16–2,000 pg/mL 88-7002


IL-17F Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.1 pg/mL 2.05–8,400 pg/mL

EPX01A-12160-901



BMS2037/2


0.13 pg/mL 2.4–10,000 pg/mL

EPX01A-26020-901



BMS6020


0.1 pg/mL 2.05–8,400 pg/mL

EPX01A-42160-901


0.4 pg/mL 4.60% 3.90% 9.77–40,000 pg/mL

EPX01A-10267-901

Platinum 50 µL 9 pg/mL 8.10% 6.50% 78–5,000 pg/mL BMS267/2*

Instant 50 µL 9.2 pg/mL 8.10% 6.50% 78–5,000 pg/mL BMS267INST*

Mouse Platinum 50 µL 10 pg/mL 2.20% 2.20% 31.3–2,000 pg/mL

BMS618/2


9.95 pg/mL 13.70% 10.50% 36.6–150,000 pg/mL

EPX01A-20618-901


0.4 pg/mL 1.95–8,000 pg/mL

EPX01A-40267-901

Porcine Platinum 50 µL 23 pg/mL 8.10% 6.80% 39.1–2,500 pg/mL

BMS672

IL-19 Mouse ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

32.6-133500 pg/mL

EPX01A-26048-901

Mouse Ready-SET-Go!®

50 μL 62.5 pg/mL 62.5–4,000 pg/mL

88-50320


Serum, Plasma: 25 µL 1.9–7,800 pg/mL EPXP010-12168-901


1.6 pg/mL 12.26–50,200 pg/mL

EPX01A-12168-901


42

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*IL-21 Human Platinum

ProcartaPlex®


EPXP010-12043-901


0.6 pg/mL 5.50% 5.40% 9.77–40,000 pg/mL

EPX01A-12043-901


Platinum 50 µL 20 pg/mL 7.70% 5.80% 78–5,000 pg/mL BMS2043


0.5 pg/mL 8.81–36,100 pg/mL

EPX01A-26021-901



BMS6021


8.2 pg/mL 3.60% 4.70% 31.74–130,000 pg/mL

EPX01A-12047-901



BMS2047




0.24 pg/mL 8.20% 9.20% 12.2–50,000 pg/mL

EPX01A-26022-901


0.9 pg/mL 2.90% 4.70% 14.65–60,000 pg/mL

EPX01A-12023-901



BMS2023/3


2.21 pg/mL 8.80% 10.40% 12.2–50,000 pg/mL

EPX01A-26017-901



BMS6017


0.9 pg/mL 6.71–27,500 pg/mL

EPX01A-42023-901

IL-25 (IL-17E) Mouse ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

4.6-19100 pg/mL EPX01A-26046-901


5.1 pg/mL 3.00% 5.40% 24.41–100,000 pg/mL

EPX01A-12085-901



BMS2085



BMS6024


0.34 pg/mL 8.70% 9.90% 2.4–10,000 pg/mL

EPX01A-26024-901

IL-28 Human Ready-SET-Go!® 50 µL 15.6 pg/mL 15.6–1,000 pg/mL

88-52102


20.31 pg/mL 5.60% 4.10% 41–168,000 pg/mL

EPX01A-26028-901


BMS6028

IL-28B (IFN lambda 3)


IL-29 (IFN lambda 1)

Human Ready-SET-Go!® 8 pg/mL 8–1,000 pg/mL 88-7296


BMS2049


28.3 pg/mL 51.83–212,300 pg/mL

EPX01A-12049-901


3.3 pg/mL 3.30% 4.80% 19.53–80,000 pg/mL

EPX01A-12041-901



0.45 pg/mL 4.60% 5.20% 7.6–31,200 pg/mL

EPX01A-26030-901


BMS6030


43


50 µL0.24 pg/mL 2.29–9,400 pg/

mLEPX01A-12048-901




BMS6025

IL-35 Human Ready-SET-Go!® 1–100 Relative Units

88-7357

IL-37 Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

1.99 pg/mL 4.22-17300 pg/mL

EPX010-12268-901

Insulin Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

195.31-800000 pg/mL

EPX010-12003-901

IP-10 (CXCL10) Human Platinum ProcartaPlex®


EPXP010-10284-901


0.3 pg/mL 5.50% 6.60% 1.95–8,000 pg/mL

EPX01A-10284-901

Instant 10 µL 1 pg/mL 10.40% 6.70% 3.1–200 pg/mL BMS284INST

Mouse Platinum 50 µL 6.5 pg/mL 7.20% 8.40% 7.8–500 pg/mL BMS6018


0.26 pg/mL 8.10% 9.50% 0.5–2,000pg/mL EPX01A-26018-901


0.9 pg/mL 7.50% 8.50% 4.9–20,100 pg/mL

EPX01A-30636-901


0.3 pg/mL 10.54–43,200 pg/mL

EPX01A-40284-901

Iso-Insulin Human Instant 50 µL 16.6 pg/mL 5.00% 4.50% 31.3–2,000 pg/mL

BMS2003INST

I-TAC (CXCL11) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

2.6 pg/mL 5.37–22,000 pg/mL

EPX01A-12124-901


2.8 pg/mL 5.63–23,100 pg/mL

EPX01A-42124-901

LAG-3 (CD223) Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

12.21-50000 pg/mL

EPX01A-12211-901

LAP (TGF beta 1) Human Ready-SET-Go!® 10 µL 156.3 pg/mL 156.3–10,000 pg/mL

88-50390

Platinum 10 µL 0.098 ng/mL 6% 7.60% 0.16–10 ng/mL BMS2065


0.96 ng/mL 3.052-12500 ng/mL

EPX010-12065-901

Mouse Ready-SET-Go!® 20 µL 0.2 ng/mL 0.2–12.5 ng/mL 88-50690

Leptin Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

2.0 pg/mL 19.31–79,100 pg/mL

EPX01A-12039-901

Instant 50 µL (1:25 prediluted) 20.0 pg/mL 6.90% 5.70% 63–4,000 pg/mL BMS2039INST


1 pg/mL 7.15–29,300 pg/mL

EPX01A-26036-901


0.4 pg/mL 16.92–69,300 pg/mL

EPX01A-32039-901

LIF Human Platinum ProcartaPlex®


EPXP010-10242-901


0.83 pg/mL 5.40% 6.40% 6.1–25,000 pg/mL

EPX01A-10242-901




BMS281*


BMS281INST*


0.28 pg/mL 5.40% 4.90% 2.4–9,850 pg/mL EPX01A-26040-901



BMS6005

Rat Instant 50 µL (1:25 prediluted) 4.7 pg/mL 11.10% 6.70% 7.8–500 pg/mL BMS631INST

LIGHT (CD258) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

2.09–8,550 pg/mL

EPX01A-12009-901


44

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*L-selectin Human ProcartaPlex® Serum, Plasma: 25 µL; CCS:

50 µL10.5 pg/mL 6.62–150,000

pg/mLEPX01A-10206-901


Instant 50 µL (1:100 prediluted) 0.19 ng/mL 10% 9.20% 0.39–25 ng/mL BMS206INST*

Mast Cell protease-1 (MCPT-1)

Mouse Ready-SET-Go!® 120 pg/mL 120–15,000 pg/mL

88-7503

MCP-1 (CCL2) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.6 pg/mL 5.80% 6.70% 1.22–5,000 pg/mL

EPX01A-10281-901


3.43 pg/mL 8.50% 12.70% 7.3–30,000 pg/mL

EPX01A-26005-901


10.9 pg/mL 6.80% 4.50% 18.3–75,000 pg/mL

EPX01A-36005-901


0.6 pg/mL 3.76–15,400 pg/mL

EPX01A-40281-901


0.7 pg/mL 6.23–25,500 pg/mL

EPX01A-50507-901

MCP-2 (CCL8) Human Platinum ProcartaPlex®


EPXP010-12169-901


0.2 pg/mL 0.49–2,000 pg/mL

EPX01A-12169-901

MCP-3 (CCL7) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

1 pg/mL 3.9–15,800 pg/mL

EPX01A-12125-901

Ready-SET-Go!® 50 µL (perdiluted 1:2) 0.3125 pg/mL 0.3125–20 pg/mL

88-50700

Platinum 50 µL 0.1 pg/mL 0.3–20 pg/mL BMS2125


0.15 pg/mL 5.10% 9.40% 0.2–1,000 pg/mL EPX01A-26006-901


BMS6006INST


1.1 pg/mL 4.60% 7.90% 3.5–14,200 pg/mL

EPX01A-36006-901


1 pg/mL 3.9–15,800 pg/mL

EPX01A-42125-901

MCP-5 (CCL12) Mouse Instant 50 µL 2.3 pg/mL 10.70% 6.70% 15.6–1,000 pg/mL

BMS6007INST

M-CSF Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

7 pg/mL 16.72–68,500 pg/mL

EPX01A-12126-901


0.02 pg/mL 4.80% 4.80% 0.3–1,100 pg/mL EPX01A-26039-901

MDC (CCL22) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.28 pg/mL 12.42–50,900 pg/mL

EPX01A-10282-901

MIF Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.06 pg/mL 0.4–1,600 pg/mL EPX01A-12127-901


0.06 pg/mL 0.4–1,600 pg/mL EPX01A-42127-901

MIG (CXCL9) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.36 pg/mL 22.2–91,100 pg/mL

EPX01A-10285-901

Instant 50 µL 4 pg/mL 11% 5.40% 31.3–2,000 pg/mL

BMS285INST


0.36 pg/mL 6.12–25,100 pg/mL

EPX01A-40285-901

MIP-1 alpha (CCL3)

Human Platinum ProcartaPlex®


EPXP010-12029-901


1.1 pg/mL 4.30% 4.20% 2.14–8,750 pg/mL

EPX01A-12029-901


BMS2029INST


0.13 pg/mL 4.80% 8.10% 0.5–2,000 pg/mL EPX01A-26013-901

Ready-SET-Go!® 50 uL 0.8 pg/mL 0.8–50 pg/mL 88-56013


1 pg/mL 6.10% 7.10% 2.3–9,500 pg/mL EPX01A-36013-901


1.1 pg/mL 6.03–24,700 pg/mL

EPX01A-42029-901


45

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*MIP-1 beta (CCL4) Human Platinum

ProcartaPlex®


EPXP010-12030-901


4.7 pg/m 4.40% 5.20% 6.1–25,000 pg/mL

EPX01A-12030-901

Instant 50 µL 3 pg/mL 4.90% 7.20% 7.8–500 pg/mL BMS2030INST


1.16 pg/mL 5.20% 9.60% 1.2–5,000 pg/mL EPX01A-26014-901


4.7 pg/mL 22.045–90,300 pg/mL

EPX01A-42030-901

MIP-2 alpha (CXCL2)


0.37 pg/mL 4.70% 4.30% 0.7–3,000 pg/mL EPX01A-26032-901


0.1 pg/mL 7.80% 6.90% 0.6–2,400 pg/mL EPX01A-36054-901

MIP-3 alpha (CCL20)


2.2 pg/mL 1.95–8,000 pg/mL

EPX01A-12128-901

MMP-1 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.04 pg/mL 7.84–32,100 pg/mL

EPX01A-12129-901


5.3 pg/mL 34.30–140,500 pg/mL

EPX01A-12132-901

MMP-3 Human Platinum 10 µL 0.008 ng/mL 3% 5.80% 0.6–4 ng/mL BMS2014/2


4.6 pg/mL 17.97–73,600 pg/mL

EPX01A-12014-901


0.04 pg/mL 7.37–30,200 pg/mL

EPX01A-12133-901


8 pg/mL 25.17–103,100 pg/mL

EPX01A-12134-901

MMP-9 Human Platinum 10 µL (1:25 prediluted) 0.05 ng/mL 10.20% 7.30% 0.23–15 ng/mL BMS2016/2*


0.2 pg/mL 0.83–3,400 pg/mL

EPX01A-12016-901


1.4 pg/mL 4.30–17,600 pg/mL

EPX01A-12130-901


2.1 pg/mL 5.76–23,600 pg/mL

EPX01A-12131-901

Myeloperoxidase (MPO)

Human Instant 50 µL (1:50 prediluted) 0.03 ng/mL 9.50% 6.20% 0.16–10 ng/mL BMS2038INST


11.9 pg/mL 122.07–500,000 pg/mL

EPX01A-12038-901

NGAL Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

4.98-20400 pg/mL

EPX01A-12202-901

NGF beta Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

6.19 pg/mL 4.30% 7.10% 7.32–30,000 pg/mL

EPX01A-12117-901


11.5 pg/mL 5.05–20,700 pg/mL

EPX01A-32117-901


6.19 pg/mL 4.30% 7.10% 7.32–30,000 pg/mL

EPX01A-42117-901


1.9 pg/mL 3.91–16,000 pg/mL

EPX01A-52117-901

Oncostatin M (OSM)


0.8 pg/mL 9.77–40,000 pg/mL

EPX01A-12163-901

Oncostatin M receptor (OSMR)

Human Ready-SET-Go!® 100 µL (1:100 prediluted) 312.5 pg/mL 312.5–20,000 pg/mL

88-50330

Osteocalcin Human Instant 25 µL 0.2 ng/mL 8.10% 8.30% 1.2–75 ng/mL BMS2020INST

Osteopontin Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

2.3 pg/ml 6.50% 9.30% 12.2–50,000 pg/mL

EPX01A-12066-901


Osteoprotegerin (OPG)

Human Platinum ProcartaPlex®


EPXP010-12021-901


3.2 pg/mL 7.3–30,000 pg/mL

EPX01A-12021-901


BMS2021INST

p53 Human Platinum 50 µL 0.33 U/mL 8.90% 5.50% 0.78–50 U/mL BMS256*Also available as CE-marked ELISA Kit

46

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*PAI-1 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS:

50 µL2.4 pg/mL 26.17–107,200

pg/mLEPX01A-12033-901

Human Platinum 50 µL (1:50 prediluted) 29.0 pg/mL 5.00% 4.70% 78–5,000 pg/mL BMS2033


0.03 pg/mL will be determinated with the first lot

EPX01A-42033-901

PD-1 (CD279) Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

6.1-25000 pg/mL EPX01A-12214-901

PD-L1 (CD274) Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

4.88-20000 pg/mL

EPX01A-12212-901

PD-L2 Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

48.83-200000 pg/mL

EPX01A-12215-901

PDGF-BB Human Platinum ProcartaPlex®


EPXP010-12071-901


6.01 pg/mL 8.10% 7.50% 7.32–30,000 pg/mL

EPX01A-12071-901

Platinum 50 µL (1:10 prediluted) 4.6 pg/mL 8.40% 4.00% 31.3–2,000 pg/mL

BMS2071


6.01 pg/mL 8.10% 7.50% 7.32–30,000 pg/mL

EPX01A-42071-901

PECAM-1 Human Platinum ProcartaPlex®

Serum, Plasma: 25 µL 126–516,100 pg/mL

EPXP010-10229-901


162.5 pg/mL 488.28–2,000,000 pg/mL

EPX01A-10229-901


PECAM-2 Human Instant 10 µL 0.06 ng/mL 4.20% 7.00% 0.47–30 ng/mL BMS229INST

PECAM-3 Monkey Instant 10 µL 0.06 U/mL 10% 2.00% 0.48–30 U/mL BMS651INST

PlGF-1 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.23 pg/mL 6.20% 8.30% 1.22–5,000 pg/mL

EPX01A-12155-901

PMN-elastase Human Platinum 100 µL (1:100 prediluted) 1.98 ng/mL 5.60% 4.80% 0.16–10 ng/mL BMS269*

P-selectin Human Platinum ProcartaPlex®

Serum, Plasma: 25 µL 990.36–4,056,500 pg/mL

EPXP010-10219-901


95.4 pg/mL 1,464.84–6,000,000 pg/mL

EPX01A-10219-901

Platinum 10 µL 0.20 ng/mL 5.40% 7.80% 0.63–400 ng/mL BMS219/4*


PSGL-1 Human Platinum 100 µL (1:20 prediluted) 1 U/mL 6.60% 3.20% 1.6–50 U/mL BMS255

PYY Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

19.53-80000 pg/mL

EPX010-12198-901

RANKL Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

2.6 pg/mL 7.76–31,800 pg/mL

EPX01A-12005-901


0.2 pg/mL 3.2–13,200pg/mL

EPX01A-26037-901


0.6 pg/mL 3.41–14,000 pg/mL

EPX01A-32005-901

RANTES (CCL5) Human Platinum ProcartaPlex®


EPXP010-10287-901


0.2 pg/mL 5.20% 7.30% 0.61–2,500 pg/mL

EPX01A-10287-901


BMS287/2INST

Mouse Instant 50 µL 1.2 pg/mL 12.10% 6.20% 7.8–500 pg/mL BMS6009INST


0.35 pg/mL 7.90% 6.80% 2.4–10,000 pg/mL

EPX01A-26009-901


6.1 pg/mL 5.60% 7.60% 85.5–350,000 pg/mL

EPX01A-36009-901


0.2 pg/mL 0.61–2,500 pg/mL

EPX01A-40287-901

RBP4 Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

195.31-800000 pg/mL

EPX01A-12205-901

Resistin (ADSF) Human Platinum 50 µL (1:10 prediluted) 3.1 pg/mL 8.10% 5.10% 31–2,000 pg/mL BMS2040


0.8 pg/mL 5.74-23,500 pg/mL

EPX01A-12040-901


0.2 pg/mL will be determinated with the first lot

EPX01A-42040-901

s90K (Mac-2BP) Human Platinum 100 µL (1:100 prediluted) 0.92 ng/mL 11.90% 5.50% 12.5–200 ng/mL BMS234

SAA Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

4.9 pg/mL 32–132,600 pg/mL

EPX01A-12136-901


47

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*SAP (Pentraxin 2) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS:

50 µL4.7 pg/mL 35.3–144,600

pg/mLEPX01A-12156-901

sAPO-1/Fas Human Platinum 10 µL 13.2 pg/mL 3.10% 4.50% 15.6–1,000 pg/mL

BMS245*

SCF Human Platinum ProcartaPlex®


EPXP010-12137-901


0.57 pg/mL 4.30% 5.90% 1.59–6,500 pg/mL

EPX01A-12137-901


0.57 pg/mL 4.30% 5.90% 1.59–6,500 pg/mL

EPX01A-42137-901


0.5 pg/mL 6.01–24,600 pg/mL

EPX01B-50508-901

SCGF beta Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

21.1 pg/mL 16.79–739,600 pg/mL

EPX01A-12157-901

SDF-1 alpha (CXCL12a)


20.5 pg/mL 4.50% 10.90% 17.09–70,000 pg/mL

EPX01A-12138-901


20.5 pg/mL 38.25–156,700 pg/mL

EPX01A-42138-901

ST2 (IL-33R) Mouse ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

24.22-99200 pg/mL

EPX01A-26047-901

Survivin (BIRC5) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

7.5 pg/mL 108.08–442,700 pg/mL

EPX01A-12158-901

TGF alpha Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.3 pg/mL 5.1–21,000 pg/mL

EPX01A-12139-901


0.3 pg/mL 5.1–21,000 pg/mL

EPX01A-42139-901

TGF beta 1 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.1 pg/mL 1.1–4,500 pg/mL EPX01A-10249-901


0.1 pg/mL 1.1–4,500 pg/mL EPX01A-20608-901


1.3 pg/mL 1.72–7,050 pg/mL

EPX01A-30249-901


0.1 pg/mL 1.1–4,500 pg/mL EPX01A-40249-901


0.1 pg/mL 1.1–4,500 pg/mL EPX01A-50249-901


0.1 pg/mL 1.1–4,500 pg/mL EPX01A-60249-901

TGF beta 2 Human Platinum 100 uL (1:50 prediluted) 6.6 pg/mL <10% <10% 31.0–1,000 pg/mL

BMS254

Thrombopoietin (TPO)


0.7 pg/mL 94.01–385,100 pg/mL

EPX01A-12161-901

TIE-2 Human Platinum 10 µL 0.03 ng/mL 6.30% 7.10% 0.16–10 ng/mL BMS2042

TIM-3 Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

61.04-250000 pg/mL

EPX01A-12219-901

TIMP-1 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

26.1 pg/mL 9.10% 9.60% 36.6–150,000 pg/mL

EPX01A-12018-901

Platinum 20 µL (1:100 prediluted) 10.0 pg/mL 3.90% 4.90% 39–2,500 pg/mL BMS2018*


Platinum 50 µL 2.3 pg/mL 7.40% 6.00% 7.8–500 pg/mL BMS2034*/ BMS223/4*


High Sensitivity




BMS607/3


BMS607/2INST

High Sensitivity


Rat Ready-SET-Go!® 16 pg/mL 16–2,000 pg/mL 88-7340

Platinum 50 µL 11 pg/mL 10% 5.00% 39.1–2,500 pg/mL

BMS622

Monkey Platinum 50 µL 6 U/mL 8.00% 8.00% 28.1–1,800 U/mL BMS654*Also available as CE-marked ELISA Kit

48

Ready-SET-Go!® ELISA; Platinum = Platinum ELISA; High Sensitivity = High Sensitivity ELISA; Instant = Instant ELISA®; InstantOne = InstantOne™ ELISA; ProcartaPlex® = ProcartaPlex® assays; ProcartaPlex® High Sensitivity = ProcartaPlex® High Sensitivity Immunoassays; Platinum ProcartaPlex® = Platinum ProcartaPlex® Immunoassays

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*TNF alpha Human ProcartaPlex®

High Sensitivity


0.215 pg/mL 4.60% 6.80% 0.45–1,830 pg/mL

EXPS010-10223-901



EPXP010-10223-901


0.4 pg/mL 6.10% 7.20% 8.54–35,000 pg/mL

EPX01A-10223-901


0.39 pg/mL 6.40% 9.80% 3.7–15,000 pg/mL

EPX01A-20607-901



0.044 pg/mL 0.34–1,390 pg/mL

EPXS010-20607-901


0.4 pg/mL 2.50% 4.20% 4.9–20,000 pg/mL

EPX01A-30622-901


0.4 pg/mL 16.64–65,800 pg/mL

EPX01A-40223-901


0.3 pg/mL 4.25–17,400 pg/mL

EPX01A-50509-901


2.27 pg/mL 6.47–26,500 pg/mL

EPX01A-66053-901

TNF beta Human Platinum 100 µL 4.6 pg/mL 10.20% 8.00% 15.6–1,000 pg/mL

BMS202*


BMS202INST*


1.6 pg/mL 0.024 0.039 6.1–25,000 pg/mL

EPX01A-10202-901


0.2 pg/mL 2.8–11,500 pg/mL

EPX01A-40202-901

TNF-R (60 kDa) Human Platinum 10 µL 0.05 ng/mL 8.60% 1.90% 0.8–5 ng/mL BMS203*

Human Instant 10 µL 53 pg/mL 8.20% 4.10% 0.78–5 ng/mL BMS203INST*

Monkey Platinum 10 µL 0.06 U/mL 9.00% 2.00% 0.9–6 U/mL BMS653

Monkey Instant 10 µL 0.059 U/mL 10% 5.00% 0.9–6 U/mL BMS653INST

TNF-R (80 kDa) Human Platinum 10 µL 0.10 ng/mL 2.00% 1.40% 0.16–10 ng/mL BMS211*


Mouse Instant 50 µL (1:50 prediluted) 0.6 pg/mL 10% 10% 7.8–500 pg/mL BMS6003INST

TNF-RI Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

79.4 pg/mL 0.056 0.078 159–650,000 pg/mL

EPX01A-10203-901

TNF-RII Human Platinum ProcartaPlex®


EPXP010-10211-901


0.1 pg/mL 1.27–5,200 pg/mL

EPX01A-10211-901

tPA Human Platinum ProcartaPlex®


EPXP010-10258-901


5 pg/mL 42.72–175,000 pg/mL

EPX01A-10258-901


BMS258/2

TRAIL Human Platinum 50 µL 5 pg/mL 7.70% 6.50% 15.6–1,000 pg/mL

BMS2004


0.6 pg/mL 12.72–52,100 pg/mL

EPX01A-12004-901


0.6 pg/mL 12.72–52,100 pg/mL

EPX01A-42004-901

TRAIL-R1 Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

19.56-80100 pg/mL

EPX01A-12011-901

TRAIL-R2 Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

19.56-80100 pg/mL

EPX01A-12010-901


49

Targets Across PlatformsTarget Species Format Volume Sensitivity Inter CV Intra CV Range Cat. No.*TSLP Human Platinum

ProcartaPlex®


EPXP010-12164-901



1.5 pg/mL 3.42–14,000 pg/mL

EPX01A-12164-901



0.7 pg/mL 8.71–35,700 pg/mL

EPX01A-26095-901

TWEAK Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

20.48–83,900 pg/mL

EPX01A-12006-901


BMS2006INST

VAP-1 Human Platinum 10 µL (1:100 prediluted) 19 pg/mL 4.50% 3.00% 31.3–2,000 pg/mL

BMS259

VCAM-1 Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

6.7 pg/mL 11.47–47,000 pg/mL

EPX01A-10232-901



1.5 pg/mL 24.93–102,100 pg/mL

EPX01A-36046-901


0.6 pg/mL 9.88–40,500 pg/mL

EPX01A-40232-901

VE-cadherin Human Instant 20 µL 0.05 ng/mL 11.80% 9.50% 0.16–10 ng/mL BMS253INST


BMS277/2*

Mouse Platinum 50 µL 20.0 pg/mL 6.40% 3.80% 62.5–4,000 pg/mL

BMS619/2

Monkey Platinum 20 µL 0.10 U/mL 10% 5.00% 0.19–12.5 U/mL BMS652

VEGF-A Human Platinum ProcartaPlex®


EPXP010-10277-901


1.73 pg/mL 3.40% 5.60% 5.86–24,000 pg/mL

EPX01A-10277-901


0.2 pg/mL 1.6–6,600 pg/mL EPX01A-20619-901


0.2 pg/mL 5.54–22,700 pg/mL

EPX01A-30619-901


1.73 pg/mL 3.40% 5.60% 5.86–24,000 pg/mL

EPX01A-40277-901


1 pg/mL 5.15–21,100 pg/mL

EPX01A-50277-901

VEGF-C Human Platinum 20 µL 0.057 ng/mL 12.00% 6.70% 0.23–15 ng/mL BMS297/2

Rat Platinum 50 µL 27.0 pg/mL 2.70% 5.10% 47–3,000 pg/mL BMS626/2

VEGF-D Human Platinum ProcartaPlex®


EPXP010-12076-901


0.88 pg/mL 4.40% 3.20% 12.21–50,000 pg/mL

EPX01A-12076-901


0.88 pg/mL 4.40% 3.20% 12.21–50,000 pg/mL

EPX01A-42076-901

VEGF-R1 (FLT-1) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

43.3 pg/mL 4.66% 7.00% 48.8–200,000 pg/mL

EPX01A-10268-901

Platinum 50 µL 0.03 ng/mL 5.10% 5.50% 0.16–10 ng/mL BMS268/3


Platinum 40 µL (pretreated) 8.6 pg/mL 4.90% 3.20% 31–2,000 pg/mL BMS249/4*


Platinum 10–40 µL (pretreated) 12 pg/mL 6.80% 6.80% 31.25–2,000 pg/mL

BMS608/4

Rat Platinum 100 µL (pretreated) 12 pg/mL 6.80% 6.80% 31.25–2,000 pg/mL

BMS623/3

VEGF-R2 (KDR) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

13.8 pg/mL 3.20% 5.00% 48.8–200,000 pg/mL

EPX01A-12019-901

Platinum 50 µL (1:25 prediluted) 7 pg/mL 9.80% 5.30% 78–5,000 pg/mL BMS2019

VEGF-R3 (FLT-4) Human ProcartaPlex® Serum, Plasma: 25 µL; CCS: 50 µL

0.066 0.08 610.35–2,500,000 pg/mL

EPX01A-12064-901

Platinum 50 µL (1:10 prediluted) 0.03 ng/mL 5.00% 5.70% 0.6–40 ng/mL BMS2064

ZAG Human ProcartaPlex® Serum, Plasma: 25 μL; CCS: 50 μL

97.66-400000 pg/m

EPX01A-12201-901


50

CE-marked Kits

Target Description Cat. No.

PMN-Elastase Human PMN-Elastase Platinum ELISA BMS269CE

sAPO-1/Fas Human sAPO-1/Fas Platinum ELISA BMS245CE

sCD23 Human sCD23 Platinum ELISA BMS227/2CE

sCD26 Human sCD26 Platinum ELISA BMS235CE

sCD30 Human sCD30 Platinum ELISA BMS240CE

Human sCD30 Instant ELISA® BMS240INSTCE

sCD40L Human sCD40L Instant ELISA® BMS239INSTCE

Human sCD40L Platinum ELISA (extra sensitive) BMS293CE

sCD44std Human sCD44std Platinum ELISA BMS209/2CE

sE-selectin Human sE-selectin Platinum ELISA BMS205CE

sHER-2 Human sHER-2 Platinum ELISA BMS207CE

sICAM-1/sCD54 Human sICAM-1 Platinum ELISA BMS201CE

sICAM-1 Human sICAM-1 Instant ELISA® BMS201INSTCE

sIL-2R/sCD25 Human sIL-2R Platinum ELISA BMS212/2CE

sIL-2R Human sIL-2R Instant ELISA® BMS212INSTCE

sIL-6R/sCD126 Human sIL-6R Instant ELISA® BMS214INSTCE

sL-selectin Human sL-selectin Platinum ELISA BMS206CE

sP-selectin Human sP-selectin Platinum ELISA BMS219/4CE

sTNF-R (60kDa) Human sTNF-R (60kDa) Instant ELISA® BMS203INSTCE

Human sTNF-R (60kDa) Platinum ELISA BMS203CE

sTNF-R (80kDa) Human sTNF-R (80kDa) Platinum ELISA BMS211CE

sVCAM-1 Human sVCAM-1 Platinum ELISA BMS232CE

TGF beta 1 Human TGF-beta1 Platinum ELISA BMS249/4CE

TIMP-1 Human TIMP-1 Platinum ELISA BMS2018CE

TNF alpha Human TNF-alpha total Platinum ELISA BMS2034CE

Human TNF-alpha Platinum ELISA BMS223/4CE

Human TNF-alpha Instant ELISA® BMS223INSTCE

TNF beta Human TNF-beta Platinum ELISA BMS202CE

Human TNF-beta Instant ELISA® BMS202INSTCE

VEGF-A Human VEGF-A Platinum ELISA BMS277/2CE

Support Products

Description Application Cat. No. Reg.

1X TMB ELISA Substrate Solution ELISA 00-4201 RUO

5X ELISA/ELISPOT Diluent ELISA 00-4202 RUO

Avidin Horseradish Peroxidase (AV-HRP) ELISA 18-4100 RUO

Cell Stimulation Cocktail (500X) FC, ELISA, FA 00-4970 GPR

ELISA Diluent Solution (5X) ELISA 00-4202 RUO

ELISA Wash Buffer - 10 x 1L Packets ELISA 00-0400 RUO

ELISA/ELISPOT Coating Buffer Powder ELISA 00-0044 RUO

InstantOne™ ELISA Cell Lysis Buffer (5X) ELISA IOCLB RUO

InstantOne™ ELISA Enhancer Solution ELISA IOES RUO

InstantOne™ ELISA Wash Buffer (10X) ELISA IOWB RUO

Nunc MaxiSorp® Flat-bottom 96-well Plate ELISA 44-2404 RUO

Substrate Solution I ELISA BMS402 RUO

Substrate Solution II ELISA BMS403 RUO

Super AquaBlue ELISA Substrate ELISA 00-4203 RUO

Support Pack Plus ELISA BMS414 RUO

Support Pack Standard ELISA BMS412 RUO

51

InstantOne ELISA™ kits

Target Description Cat. No.

AKT Pathway Activation Profile AKT Pathway Activation Profile InstantOne ELISA™ kit 85-86048

Phospho-AKT 1/2/3 (Ser473) Phospho-AKT 1/2/3 (Ser473) InstantOne ELISA™ kit 85-86042

AKT 1 (Total) AKT 1 (Total) InstantOne ELISA™ kit 85-86047

AKT (Total/Phospho) AKT (Total/Phospho) InstantOne ELISA™ kit 85-86046

Phospho-AKT 1/2/3 (Thr308) Phospho-AKT 1/2/3 (Thr308) InstantOne ELISA™ kit 85-86044

AKT/ERK Activation AKT/ERK Activation InstantOne ELISA™ kit 85-86014

ERK/AKT/p70 S6K Activation ERK/AKT/p70 S6K Activation InstantOne ELISA™ kit 85-86018

AKT Activation AKT Activation InstantOne ELISA™ kit 85-86049

Beta Catenin (Total/Phospho) Beta Catenin (Total/Phospho) InstantOne ELISA™ kit 85-86143

Beta Catenin (Total) Beta Catenin (Total) InstantOne ELISA™ kit 85-86141

Phospho-beta-Catenin (Ser45) Phospho-beta-Catenin (Ser45) InstantOne ELISA™ kit 85-86142

CREB (Total/Phospho) CREB (Total/Phospho) InstantOne ELISA™ kit 85-86153

CREB (Total) CREB (Total) InstantOne ELISA™ kit 85-86151

Phospho-CREB (Ser133) Phospho-CREB (Ser133) InstantOne ELISA™ kit 85-86152

ERK 1/2 (Total/Phospho) ERK 1/2 (Total/Phospho) InstantOne ELISA™ kit 85-86013

ERK 1/2 (Total) ERK 1/2 (Total) InstantOne ELISA™ kit 85-86011

Phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187)Phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) InstantOne ELISA™ kit

85-86012

GAPDH GAPDH InstantOne ELISA™ kit 85-86131

Phospho-GSK3 beta (Ser9) Phospho-GSK3 beta (Ser9) InstantOne ELISA™ kit 85-86172

GSK3 beta (Total) GSK3 beta (Total) InstantOne ELISA™ kit 85-86171

GSK3 beta (Total/Phospho) GSK3 beta (Total/Phospho) InstantOne ELISA™ kit 85-86173

I kappa B alpha (Total/Phospho) I kappa B alpha (Total/Phospho) InstantOne ELISA™ kit 85-86063

I kappa B alpha (Total) I kappa B alpha (Total) InstantOne ELISA™ kit 85-86061

Phospho-I kappa B alpha (Ser32/36) Phospho-I kappa B alpha (Ser32/36) InstantOne ELISA™ kit 85-86062

Phospho-IKK alpha (Ser176/180) Phospho-IKK alpha (Ser176/180) InstantOne ELISA™ kit 85-86072

IKK alpha (Total) IKK alpha (Total) InstantOne ELISA™ kit 85-86071

IKK alpha (Total/Phospho) IKK alpha (Total/Phospho) InstantOne ELISA™ kit 85-86073

JNK 1/2 (Total/Phospho) JNK 1/2 (Total/Phospho) InstantOne ELISA™ kit 85-86198

Phospho-JNK 1/2 (Thr183/Tyr185) Phospho-JNK 1/2 (Thr183/Tyr185) InstantOne ELISA™ kit 85-86197

JNK 1/2 (Total) JNK 1/2 (Total) InstantOne ELISA™ kit 85-86196

c-Jun (Total) c-Jun (Total) InstantOne ELISA™ kit 85-86161

Phospho-c-Jun (Ser73) Phospho-c-Jun (Ser73) InstantOne ELISA™ kit 85-86162

c-Jun (Total/Phospho) c-Jun (Total/Phospho) InstantOne ELISA™ kit 85-86163

MAPK Family (ERK, p38, JNK) Activation MAPK Family (ERK, p38, JNK) Activation InstantOne ELISA™ kit 85-86195

NF kappa B p65 (Total/Phospho) NF kappa B p65 (Total/Phospho) InstantOne ELISA™ kit 85-86083

Phospho-NF kappa B p65 (Ser536) Phospho-NF kappa B p65 (Ser536) InstantOne ELISA™ kit 85-86082

NF kappa B p65 (Total) NF kappa B p65 (Total) InstantOne ELISA™ kit 85-86081

NF kappa B Pathway Activation NF kappa B Pathway Activation InstantOne ELISA™ kit 85-86084

p38 MAPK (Total/Phospho) p38 MAPK (Total/Phospho) InstantOne ELISA™ kit 85-86023

p38 MAPK (Total) p38 MAPK (Total) InstantOne ELISA™ kit 85-86021

Phospho-p38 MAPK (Thr180/Tyr182) Phospho-p38 MAPK (Thr180/Tyr182) InstantOne ELISA™ kit 85-86022

p53 (Total/Phospho) p53 (Total/Phospho) InstantOne ELISA™ kit 85-86123

p53 (Total) p53 (Total) InstantOne ELISA™ kit 85-86121

p70 S6K (Total/Phospho) p70 S6K (Total/Phospho) InstantOne ELISA™ kit 85-86053

Phospho-p70 S6K (Thr389) Phospho-p70 S6K (Thr389) InstantOne ELISA™ kit 85-86052

p70 S6K (Total) p70 S6K (Total) InstantOne ELISA™ kit 85-86051

Phospho-STAT1 (Tyr701) Phospho-STAT1 (Tyr701) InstantOne ELISA™ kit 85-86092

Phospho-S6 RP (Ser240/244) Phospho-S6 RP (Ser240/244) InstantOne ELISA™ kit 85-86194

Phospho-SMAD1 (Ser463/465) Phospho-SMAD1 (Ser463/465) InstantOne ELISA™ kit 85-86182

10255 Science Center Drive

San Diego, CA 92121 USA

www.affymetrix.com Please visit our website for international distributor contact information.For Research Use Only. Not for use in diagnostic procedures.

© 2016 Affymetrix, Inc. All rights reserved. Affymetrix®, eBioscience®, BestProtocols®, eFluor®, eVolve™, eZkine®, Full Spectrum Cell Analysis™, Instant ELISA®, InstantOne ELISA™, MagniSort™, OneComp eBeads®, 123count eBeads®, PrimeFlow®, Procarta®, ProcartaPlex®, QuantiGene®, Ready-SET-Go!®, SAFE™, The New Standard of Excellence®, UltraComp eBeads®, and ViewRNA® are trademarks or registered trademarks of Affymetrix, Inc. Please see affymetrix.com/trademarks for a complete list of Affymetrix trademarks. All other trademarks are the property of their respective owners.

P/N IMM06171 Immunoassay Resource Guide Rev 0416

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