Immuno Fluorescence
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FLOW CYTOMETRY
description
details of immunofluorescense
Transcript of Immuno Fluorescence
FLOW CYTOMETRY
Flow cytometry is a technology that simultaneously measures, and then analyses multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light, usually a laser.
DEFINITION
What can a flow cytometer tell us about a cell Its relative size (Forward Scatter—FSC)Its relative granularity or internal complexity (Side Scatter—SSC) Its relative fluorescence intensity (FL1, FL2, FL3, and FL4)
BASICS OF FLOW CYTOMETRY
Components of FLOW CYTOMETRY:
