Immediate and long term skin benefits of an after sun treatment containing non-denatured total soy...
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P530 A RE-ASSESSMENT OF THE PROCEDURES FOR PREDECTING THE PHOTOSENSITI- ZATION POTENTIAL OF TOPICAL PRODUCTS Monya L Sigler, PhD, Thomas J. Stephens & Associates, Inc., Carrolloton, TX, United States, Thomas Stephens, PhD, Thomas J. Stephens & Associates, Inc., Carrolloton, TX, United States, Ronald Rizer, PhD, Thomas J. Stephens & Associates, Inc., Carrolloton, TX, United States, Otto Mills, PhD, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, NJ, United States Current procedures used to predict the phototoxic and photoallergic potential of topical products in humans have their origin in experimental protocols for producing photosen- sitization reactions in guinea pigs. In response to the outbreak of photosensitivity observerd in the 1960s and 1970s, these procedures were modified into human predic- tive tests. In an attempt to enhance the sensitivity of detecting photoallergens, some investigators began manipulating induction procedures by exposing test subjects to blistering doses of ultraviolet light B (UVB). The theory behind this procedure was that repeated exposures to erythematogenic radiation would induce an inflammatory reaction and compromise the stratum corneum. In recent years, other investigators have demon- strated that excessive exposure to UVB induces alterations in Langerhans cells resulting in down regulation of the immune system. This resulted in a reduced ability to induce allergic contact dermatitis (ACD) and photo-ACD reactions. In spite of this observation many current human photoallergy testing protocols continue to expose subjects to excessive amounts of UVB during the induction phase. In this poster we describe a modified human testing approach in which a topically applied agent can be screened for both phototoxicity and photoallergy in the same test with reduced levels of UVB exposure. Disclosure not available at press time. P531 COSTING NARROW-BAND UVB PHOTOTHERAPY IN PSORIASIS Sinead M Langan, MD, St James’s Hospital, Dublin 8, Ireland, Adrienne Heerey, BSc, Michael Barry, MD, Louise Barnes, MD Background: There is little data available to health care providers regarding the costs of treating psoriasis patients, and specifically the cost of phototherapy. Objectives: As narrowband UVB (TL-01) has now become an established therapy for psoriasis patients requiring phototherapy, we determined the annual cost of delivering TL-01 treatment in a university hospital. Methods: The costing evaluation was from a hospital perspective and the strategy used was a microcosting detailed collection of resources used. Results: The annual cost of TL-O1 treatment in our teaching hospital is 53, 555.00 Euro. Staffing accounts for 70% of the cost. The average individual costs were 325.00 Euro (range 57.20 to 972.40 Euro). These costs are significant but remain less expensive than inpatient treatment. No conflicts of interest to disclose. P532 EICOSANOID RECEPTORS SHOWED DOWN-REGULATION AFTER UVA IRRADIA- TION OF ATOPIC DERMATITIS LESIONAL KERATINOCYTES Peter Arenberger, MD, PhD, Dept. of Dermatology, Charles University School of Medi- cine, Prague 10, Czech Republic, Iva Obstova, MD, Dept. of Dermatology, Charles University School of Medicine, Prague 10, Czech Republic, Daniela Blahova, MD, Dept. of Dermatology, Charles University School of Medicine, Prague 10, Czech Republic, Lenka Paralova, MD, Dept. of Dermatology, Charles University School of Medicine, Prague 10, Czech Republic Different changes in skin after UV-irradiation has been described and visualized by electron microscopy. Apoptosis or DNA-damage are well-known as disturbances caused by UV-radiation. On the cell surface, receptors could be also affected during treatment with artificial UV sources or during skin irradiation for cosmetic reasons. Because 12-hydroxyeicosatetraenoic acid (12-HETE) is considered to be the main epidermal eicosanoid, and is assumed to have both pathophysiological effects in inflammatory skin diseases such as atopic dermatitis as well as a physiological role in cutaneous biology, we decided to show the UV-light effect on 12-HETE surface receptors in atopic dermatitis keratinocytes. Therefore, the present work studied the effects of single and repeated irradiations with UVA light from a Waldmann 100 W bulb on the 12(S)-HETE receptors in skin keratino- cytes obtained from lesional skin of patients with atopic dermatitis. UV-light (50-150 J/m2) induced a down-regulation of 12(S)HETE receptors in a dose-dependent manner. The above described effect occurred after a latency period of 6 h and reached its maximum at 7.5 h. A single UV irradiation (150 J/m2) or repeated irradiation (50 J/m2) developed a 55% receptor down-regulation (Bmax) with no significant impact on the receptor affinity (Kd). This work was supported by a Charles University Grant-69/2002. Disclosure not available at press time. P533 IMMEDIATE AND LONG TERM SKIN BENEFITS OF AN AFTER SUN TREATMENT CONTAINING NON-DENATURED TOTAL SOY EXTRACT Theresa Chen, PhD, Neutrogena Corporation, Los Angeles, CA, United States, Claude Saliou, PhD, Johnson & Johnson Consumers and Personal Products Worldwide, Skillman, NJ, United States, Ronald Rizer, PhD, Thomas J. Stephens & Associates, Carrollton, TX, United States, Yohini Appa, PhD, Neutrogena Corporation, Los Angeles, CA, United States Exposure to ultraviolet light (UV) leads to the immediate effects of erythema, pigment darkening, dryness and peeling of the skin, while long-term repeated UV exposure can result in photo-damage. After-sun treatments can help alleviate the dryness and peeling but most do not address the other effects of UV exposure. Non-denatured total soy extract has been shown to offer many anti-aging/photo-aging benefits. Thus non-denatured total soy extract has been formulated into an after sun moisturizing treatment. The soy- containing product had superior antioxidant activity over placebo and also inhibited trypsin activity in vitro. The benefits of this after-sun soy treatment on UV exposed skin were evaluated on Fitzpatrick skin photo-types I–IV in placebo controlled clinical trials. The results showed that the after-sun soy treatment was able to diminish the immediate effects of sun exposure by reducing the erythema and pigment darkening resulting immediately following UV exposure, in addition to improving skin moisturization, rough- ness (texture), flaking and the appearance of crepiness. Continued usage showed that the after sun soy treatment was significantly superior to placebo in reducing the signs of fine lines, mottled pigmentation and laxity. These findings are supported by clinical grading and subject self-assessment questionnaires. Non-invasive bioengineering methods using direct reflectance spectroscopy and mexameter further confirmed less erythema and melanin increases on the treated sites than on the untreated sites. Taken together, these results show that the after sun soy treatment is able to ameliorate many of the short-term and long-term effects of UV exposure. The presenters are either employees of the manufacturer or its affiliates or contract test facility. 100% is sponsored by Neutrogena Corporation. P137 MARCH 2004 JAM ACAD DERMATOL
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Transcript of Immediate and long term skin benefits of an after sun treatment containing non-denatured total soy...
P530A RE-ASSESSMENT OF THE PROCEDURES FOR PREDECTING THE PHOTOSENSITI-ZATION POTENTIAL OF TOPICAL PRODUCTSMonya L Sigler, PhD, Thomas J. Stephens & Associates, Inc., Carrolloton, TX, UnitedStates, Thomas Stephens, PhD, Thomas J. Stephens & Associates, Inc., Carrolloton, TX,United States, Ronald Rizer, PhD, Thomas J. Stephens & Associates, Inc., Carrolloton, TX,United States, Otto Mills, PhD, UMDNJ-Robert Wood Johnson Medical School, NewBrunswick, NJ, United States
Current procedures used to predict the phototoxic and photoallergic potential of topicalproducts in humans have their origin in experimental protocols for producing photosen-sitization reactions in guinea pigs. In response to the outbreak of photosensitivityobserverd in the 1960s and 1970s, these procedures were modified into human predic-tive tests. In an attempt to enhance the sensitivity of detecting photoallergens, someinvestigators began manipulating induction procedures by exposing test subjects toblistering doses of ultraviolet light B (UVB). The theory behind this procedure was thatrepeated exposures to erythematogenic radiation would induce an inflammatory reactionand compromise the stratum corneum. In recent years, other investigators have demon-strated that excessive exposure to UVB induces alterations in Langerhans cells resultingin down regulation of the immune system. This resulted in a reduced ability to induceallergic contact dermatitis (ACD) and photo-ACD reactions. In spite of this observationmany current human photoallergy testing protocols continue to expose subjects toexcessive amounts of UVB during the induction phase. In this poster we describe amodified human testing approach in which a topically applied agent can be screened forboth phototoxicity and photoallergy in the same test with reduced levels of UVBexposure.
Disclosure not available at press time.
P531COSTING NARROW-BAND UVB PHOTOTHERAPY IN PSORIASISSinead M Langan, MD, St James’s Hospital, Dublin 8, Ireland, Adrienne Heerey, BSc,Michael Barry, MD, Louise Barnes, MD
Background: There is little data available to health care providers regarding the costs oftreating psoriasis patients, and specifically the cost of phototherapy.
Objectives: As narrowband UVB (TL-01) has now become an established therapy forpsoriasis patients requiring phototherapy, we determined the annual cost of deliveringTL-01 treatment in a university hospital.
Methods: The costing evaluation was from a hospital perspective and the strategy usedwas a microcosting detailed collection of resources used.
Results: The annual cost of TL-O1 treatment in our teaching hospital is 53, 555.00 Euro.Staffing accounts for 70% of the cost. The average individual costs were 325.00 Euro(range 57.20 to 972.40 Euro). These costs are significant but remain less expensive thaninpatient treatment.
No conflicts of interest to disclose.
P532EICOSANOID RECEPTORS SHOWED DOWN-REGULATION AFTER UVA IRRADIA-TION OF ATOPIC DERMATITIS LESIONAL KERATINOCYTESPeter Arenberger, MD, PhD, Dept. of Dermatology, Charles University School of Medi-cine, Prague 10, Czech Republic, Iva Obstova, MD, Dept. of Dermatology, CharlesUniversity School of Medicine, Prague 10, Czech Republic, Daniela Blahova, MD, Dept. ofDermatology, Charles University School of Medicine, Prague 10, Czech Republic, LenkaParalova, MD, Dept. of Dermatology, Charles University School of Medicine, Prague 10,Czech Republic
Different changes in skin after UV-irradiation has been described and visualized byelectron microscopy. Apoptosis or DNA-damage are well-known as disturbances causedby UV-radiation. On the cell surface, receptors could be also affected during treatmentwith artificial UV sources or during skin irradiation for cosmetic reasons. Because12-hydroxyeicosatetraenoic acid (12-HETE) is considered to be the main epidermaleicosanoid, and is assumed to have both pathophysiological effects in inflammatory skindiseases such as atopic dermatitis as well as a physiological role in cutaneous biology, wedecided to show the UV-light effect on 12-HETE surface receptors in atopic dermatitiskeratinocytes.
Therefore, the present work studied the effects of single and repeated irradiations withUVA light from a Waldmann 100 W bulb on the 12(S)-HETE receptors in skin keratino-cytes obtained from lesional skin of patients with atopic dermatitis. UV-light (50-150J/m2) induced a down-regulation of 12(S)HETE receptors in a dose-dependent manner.The above described effect occurred after a latency period of 6 h and reached itsmaximum at 7.5 h. A single UV irradiation (150 J/m2) or repeated irradiation (50 J/m2)developed a 55% receptor down-regulation (Bmax) with no significant impact on thereceptor affinity (Kd). This work was supported by a Charles University Grant-69/2002.
Disclosure not available at press time.
P533IMMEDIATE AND LONG TERM SKIN BENEFITS OF AN AFTER SUN TREATMENTCONTAINING NON-DENATURED TOTAL SOY EXTRACTTheresa Chen, PhD, Neutrogena Corporation, Los Angeles, CA, United States, ClaudeSaliou, PhD, Johnson & Johnson Consumers and Personal Products Worldwide, Skillman,NJ, United States, Ronald Rizer, PhD, Thomas J. Stephens & Associates, Carrollton, TX,United States, Yohini Appa, PhD, Neutrogena Corporation, Los Angeles, CA, United States
Exposure to ultraviolet light (UV) leads to the immediate effects of erythema, pigmentdarkening, dryness and peeling of the skin, while long-term repeated UV exposure canresult in photo-damage. After-sun treatments can help alleviate the dryness and peelingbut most do not address the other effects of UV exposure. Non-denatured total soy extracthas been shown to offer many anti-aging/photo-aging benefits. Thus non-denatured totalsoy extract has been formulated into an after sun moisturizing treatment. The soy-containing product had superior antioxidant activity over placebo and also inhibitedtrypsin activity in vitro. The benefits of this after-sun soy treatment on UV exposed skinwere evaluated on Fitzpatrick skin photo-types I–IV in placebo controlled clinical trials.The results showed that the after-sun soy treatment was able to diminish the immediateeffects of sun exposure by reducing the erythema and pigment darkening resultingimmediately following UV exposure, in addition to improving skin moisturization, rough-ness (texture), flaking and the appearance of crepiness. Continued usage showed that theafter sun soy treatment was significantly superior to placebo in reducing the signs of finelines, mottled pigmentation and laxity. These findings are supported by clinical gradingand subject self-assessment questionnaires. Non-invasive bioengineering methods usingdirect reflectance spectroscopy and mexameter further confirmed less erythema andmelanin increases on the treated sites than on the untreated sites. Taken together, theseresults show that the after sun soy treatment is able to ameliorate many of the short-termand long-term effects of UV exposure.
The presenters are either employees of the manufacturer or its affiliates or contract testfacility.
100% is sponsored by Neutrogena Corporation.
P137MARCH 2004 J AM ACAD DERMATOL
