Image-Pro Premier Basic Training Course
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Transcript of Image-Pro Premier Basic Training Course
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Image-Pro PremierBasic Training Course
Part 7 - Image Enhancements
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Image Enhancements
Enhancements can be made for several reasons:AestheticsFunctionalityPracticality
If the image is modified, the process must be:SystematicJustifiable
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Always make a copy of the image before
processing!
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Display Enhancement
Use Look-Up Tables to adjust how pixels are displayed on the screenNon-destructive unless appliedDoesn’t generally improve ability to analyse images
![Page 5: Image-Pro Premier Basic Training Course](https://reader035.fdocuments.in/reader035/viewer/2022062304/56813253550346895d98d56e/html5/thumbnails/5.jpg)
Filters
Process individual pixels based on their environmentUsed to:
enhance or reduce noisePull out EdgesEnhance morphological feature
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Masks
Create binary images for processingCan be used to extract further information from objectsUse from Count/Size to fill holes or add smoothing
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Colour Extraction
Colour increases the complexity of analysis
Colour isn’t always necessary or useful
Colour images can be converted to mono from the Adjust ribbon by either:
Extracting colour channels
Averaging information from all channels
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Colour Extraction
Mono Average
Colour Separation
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Background Correction
Uneven background can be aesthetically jarring and render analysis difficult
Use background Correction to flatten backgrounds by:
Subtracting background image
Estimating background
![Page 10: Image-Pro Premier Basic Training Course](https://reader035.fdocuments.in/reader035/viewer/2022062304/56813253550346895d98d56e/html5/thumbnails/10.jpg)
Colour Composites
Fluorescence imaging is typically monochromeMultiple images are taken separately from the same sampleColour composites recombine multiple monochrome images into a single workspace