IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los...
-
date post
21-Dec-2015 -
Category
Documents
-
view
219 -
download
0
Transcript of IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los...
![Page 1: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/1.jpg)
IM-SURE Fellow: Georges SiddiqiDepartment of Chemical Engineering University of California, Los Angeles
Mentor: Professor Regina RaganPost-Doctoral Researcher: Dr. Ju H. ChoiDepartment of Chemical Engineering and Materials ScienceUniversity of California, Irvine
Study of Self Assembled Nanoparticle Arrays on
Diblock Copolymer Templates
![Page 2: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/2.jpg)
Outline
•Our motivations•Introduction to concepts•Project goals•Experimental outline•Results and discussion
![Page 3: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/3.jpg)
Motivation
• Create biosensors that are both• Cost effective• Highly sensitive
• Existing biosensors are large, mechanically complicated and expensive
•Difficult, time consuming and expensive to detect many biological molecules
![Page 4: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/4.jpg)
Introduction
•Use plasmon resonance spectroscopy to create highly sensitive biosensors
Van Duyne R.P. et al, Nano Lett., Vol. 4, No. 6, 2004
![Page 5: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/5.jpg)
•Diblock copolymers form two distinct phases depending on fraction of each
•Using poly(styrene-b-methyl methacrylate) (PS-b-PMMA)
Introduction
![Page 6: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/6.jpg)
Introduction
•Combine diblock copolymer templates with nanoparticles
PMMA PMMAPS
Creates cost effective templates
•Combine nanoparticle arrays with plasmon resonance spectroscopy
Creates highly sensitive biosensor
![Page 7: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/7.jpg)
PMMA
Polymersubstrate
Project Goals
•Three goals for this project:•Create size-controlled nanoparticles•Create diblock copolymer•Attach nanoparticles
Monolayer protected Au/Ag nanoparticle synthesis
Diblock-copolymer templates (PS-b-PMMA)
(What I did)
![Page 8: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/8.jpg)
•Convert –COOCH3 group to carboxylic acid
Experimental Outline
•React with EDC and Sulfo-NHS to form amine reactive ester•Attach functionalized nanoparticle
![Page 9: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/9.jpg)
Results
•Initial Results
![Page 10: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/10.jpg)
Results
•Complications with morphology
![Page 11: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/11.jpg)
Results
Spin speed &Conc. of PS-b-PMMA
3000 rpm 4000 rpm 5000 rpm
1 wt% 55nm(70%)
50nm (60%)
49nm (64%)
2 wt% 88nm(92%)
88nm(87%)
85nm(75%)
•Control of film thickness
![Page 12: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/12.jpg)
Results
•Problems with template damage and their solutions
![Page 13: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/13.jpg)
Results
•Problems with nanoparticle aggregation
![Page 14: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/14.jpg)
Results
•Controlling nanoparticle size
Expected NP size ~10nm Expected NP size ~20nm
![Page 15: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/15.jpg)
Results
•Finally some good stuff
![Page 16: IM-SURE Fellow: Georges Siddiqi Department of Chemical Engineering University of California, Los Angeles Mentor: Professor Regina Ragan Post-Doctoral Researcher:](https://reader030.fdocuments.in/reader030/viewer/2022033103/56649d575503460f94a35f14/html5/thumbnails/16.jpg)
Acknowledgements
•Professor Regina Ragan•Dr. Ju H. Choi•Jere. A Wilson for some bangin’ NPs•UCI IM-SURE Program•NSF REU Program•Carl Zeiss Center of Excellence