Il codice genetico Il dogma centrale della Biologia DNA RNA proteina trascrizione inversa traduzione...
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Transcript of Il codice genetico Il dogma centrale della Biologia DNA RNA proteina trascrizione inversa traduzione...
Il codice genetico
Il dogma centrale della Biologia
DNA
RNA
proteina
trascrizionetrascrizione
inversa
traduzione
41= 4 possibilità
42=16 possibilità
43=64 possibilità
20 aminoacidi
5’
3’
N-terminus C-terminus
The sequence of a coding strand of DNA, read in the direction from 5’ to 3’, consists of nucleotide triplets (codons) corresponding to the amino acid sequence of a protein read from N-terminus to C-terminus.
Il codice genetico è letto a triplette
Analisi dei mutanti nel locus rII del fago T4 (F. Crick and S. Brenner, 1961).
Ceppo T4 Ceppo E. coli B KrII- grandi, rotonde no placcherII+ piccole, irregolari piccole, irregolari
(studio della ricombinazione genica, Benzer, 1950)
Induzione del fenotipo rII- mediante l’uso di proflavina che provocava inserzioni o delezioni di singole coppie di basi.
A
G
Studiando una particolare mutazione indotta dalla proflavina, denominata FCO, Crick e collaboratori trovarono delle reversioni rivelate dalla formazione di placche normali su cellule del ceppo di E. coli K.
L’analisi genetica rivelò che i revertanti non erano identici al wild type. La retromutazione non era un’esatta inversione della mutazione originale ed era dovuta ad una seconda mutazione in un sito diverso.
wt CAU CAU CAU CAU CAU
Insersione CAU ACA UCA UCA UCA
Delezione CAU ACA UCA UCA UCA
CAU ACA UAU CAU CAU
Sistemi di sintesi proteica cell-free (M. Nirenberg, 1961)
Incorporazione di aminoacidi radioattivi nelle proteine in un sistema in vitro
La prima grande conquista fu la scoperta del procedimento per ottenere mRNA sintetico. Se si mescolano i ribonucleotidi ad elevata conc. con l’enzima polinucleotide fosforilasi la reazione produce RNA a singolo filamento (la sintesi non richiede il DNA stampo e i nucleotidi sono incorporati a caso).
1) Poli (U) mRNA polifenilalanina
2) Poli (A) mRNA polilisina
3) Poli (C) mRNA poliprolina
4) Poli (G) mRNA no traduzione (H-DNA)
La Decifrazione del Codice Genetico
5) Polimeri casuali di A e C in cui:
[A]>[C] polipeptide composto da Asn e Gln (2A+C)
[C]>[A] polipeptide composto da His e Thr (2C+A)
6) Poli (CA) polipeptide risultante: Thr-His-Thr-His
7) Poli (UC) polipeptide risultante: Leu-ser-Leu-Ser
Nel 1963 Nirenberg e Matthaei avevano identificato l’aminoacido associato con 40 codoni (composizione ma non l’ordine dei nucleotidi)
Ribosome binding assay (Nirenberg and Leder, 1965)
+ + X-X-XTrinucleotide
da saggiare
(seq. nota)
Filtro di nitrocellulosaSolo il complesso ribosomaaminoacil-tRNA (corrispondende al codone in esame)trinucleodite è trattenuto sul filtro mentre gli aminoacil-tRNA liberi non vengono trattenuti
61 codons represent amino acids and 3 cause termination (TERM)
The reduced specificity at the last position is known as third-base degeneracy.Often one tRNA can recognize more than one codon. This means that the base in the first position of the anticodon must be able to partner alternative bases in the corresponding third position of the codon.
The fMet-tRNA is used only for initiation. It recognizes AUG (Met) or GUG (Val) and occasionally UUG (Leu). The extent of initiation declines about half when AUG is replaced by GUG and by about half yet again when UUG is employed.
Impiego del codice genetico e dei codoni in E. coli e nell’uomo
Mutation amino acid
CUC CUG no effect (Leu)
CCU AUU Leu Ile
UUU UCU Phe Tyr
UUC UAC Phe Tyr
The number of codons for each amino acid does not correlate with the frequency of use in proteins
L’ RNA transfer
74-95 nucleotidi
There are 20 aminoacyl-tRNA synthetase, each recognizing a single amino acid and all the tRNAs onto which it can legitimately be placed.
= pseudouridinamG e m2G= metil e dimetilguanosinamI= metilinosinaDHU o UH2= diidrouridina
Once a tRNA has been charged, the amino acid plays no further role in its specificity, which is determined exclusively by the anticodon
A group of isoaccepting tRNAs must be charged only by the single aminoacyl-tRNA synthetase specific for their amino acid. There are 20 aminoacyl-tRNA synthetase in the cell.
Nonsense mutations suppression(intergenic suppression)
There are 3 separate genes coding for tRNATyr
Frameshift mutations suppression(intragenic suppression)
Stop codon
Base mutata
Often one tRNA can recognize more than one codon, codon-anticodon recognition involves wobbling.There are only three cases in which a unique meaning is conferred by the presence of a particular base at the third position: AUG (for methionine), UGG (for tryptophan), and UGA (termination).
Wobbling occurs because the conformation of the tRNA anticodon loop permits flexibility at the first base of the anticodon.
Phe
Leu
Leu
Ile
Val
Ser
Pro
Thr
Ala
Met
Tyr
His
Gln
Asn
Lys
Asp
Glu
Cys
Trp
Arg
Ser
Arg
Gly
anticodon
codon
stop
Leu, Arg and Ser have 3 different tRNAs
Modifications occur in all parts of the tRNA molecule. There are more than 50 different types of modified bases in tRNA (D, T and were originally transcribed as U while mG, dmG, I and mI were transcribed as G).
When bases in the anticodon are modified, further pairing patterns become possible in addition to those predicted by the regular and wobble pairing involving A, C, U, and G.
Ile codons
AUA
AUU
AUC
Anticodon UAI
-NH2
-NH2
According to wobbling rules a single tRNA can not recognize four codons
Some modifications create preferential readings of some codons 1) 2-thiouracil pairs only with A 2) Anticodons with uridine-5-oxyacetic acid and 5-methoxyuridine in the first position recognize A and G efficiently as third bases of the codon, but recognize U less efficiently3) Queuosine and its derivatives (modified G bases) continue to recognize both C and U, but pair with U more readily.
There are sporadic alterations of the universal codeThe universality of the genetic code is striking, but some exceptions exist. They tend to affect the codons involved in initiation or termination
Exceptions to the universal genetic code also occur in the mitochondria from several species (they have been evolved separately).
UC = Universal Code, the anticodon is in parenthesis
Arg (UC)
Ile (UC)
Changes of codon meaning in Mammaliam Mitochondria
Stop (UC)