If you have a Cell Project on the front counter, please take it now (put on hallway table and take...
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Transcript of If you have a Cell Project on the front counter, please take it now (put on hallway table and take...
If you have a Cell Project on the front counter, please take it now (put on hallway table and
take it after class).Materials: ISN
1. Define extraction in your own words. 2. How do you suppose we will extract DNA
from strawberries and peas?3. What are your predictions for how DNA
will look?
DNA ExtractionDNA is the genetic code-of lifeDNA is found in the chromosomesChromosomes are found in the nucleusAll eukaryotic cells have a nucleusAll living organisms are made of cells
DNA Extraction - StepsCollect cellsBurst cell membranes open - saltDissolve lipid (fat) layer - soapSeparate DNA from proteins and debris - saltIsolate concentrated DNA – 91% Isopropyl
Alcohol (stored in freezer)
Take the green part off the strawberry
Add the strawberry, 10 ml. water, and 3 pinches of salt to the baggy.
“Smoosh” the strawberry with your fingers
Use the stick to get a drop of soap
Add a drop of soap to the baggy
Gently rock the baggy back-and-forth (so you don’t make bubbles)
Put the coffee filter into the funnel
Slowly pour the strawberry juice into the filter
It takes time to filter through
Pour the juice into the smaller beaker or test tube
Tilt both containers when you pour the alcohol down the side
Pull the DNA out with the pipette
Drawing Conclusions1. Why was it necessary to add salt to the
mixture?2. Why was it necessary to add soap to the
mixture?3. What did the DNA look like? 4. Explain how DNA is stored in the nucleus.5. Explain what happened in the final step
when you added the alcohol.6. List two reasons why it is important for
scientists to remove DNA from an organism.