If you have a Cell Project on the front counter, please take it now (put on hallway table and take...

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If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN

Transcript of If you have a Cell Project on the front counter, please take it now (put on hallway table and take...

Page 1: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

If you have a Cell Project on the front counter, please take it now (put on hallway table and

take it after class).Materials: ISN

Page 2: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

1. Define extraction in your own words. 2. How do you suppose we will extract DNA

from strawberries and peas?3. What are your predictions for how DNA

will look?

Page 3: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

DNA ExtractionDNA is the genetic code-of lifeDNA is found in the chromosomesChromosomes are found in the nucleusAll eukaryotic cells have a nucleusAll living organisms are made of cells

Page 4: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

DNA Extraction - StepsCollect cellsBurst cell membranes open - saltDissolve lipid (fat) layer - soapSeparate DNA from proteins and debris - saltIsolate concentrated DNA – 91% Isopropyl

Alcohol (stored in freezer)

Page 5: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.
Page 6: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

Take the green part off the strawberry

Page 7: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

Add the strawberry, 10 ml. water, and 3 pinches of salt to the baggy.

Page 8: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

“Smoosh” the strawberry with your fingers

Page 9: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

Use the stick to get a drop of soap

Page 10: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

Add a drop of soap to the baggy

Page 11: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

Gently rock the baggy back-and-forth (so you don’t make bubbles)

Page 12: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

Put the coffee filter into the funnel

Page 13: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

Slowly pour the strawberry juice into the filter

Page 14: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

It takes time to filter through

Page 15: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

Pour the juice into the smaller beaker or test tube

Page 16: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.
Page 17: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

Tilt both containers when you pour the alcohol down the side

Page 18: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

Pull the DNA out with the pipette

Page 19: If you have a Cell Project on the front counter, please take it now (put on hallway table and take it after class). Materials: ISN.

Drawing Conclusions1. Why was it necessary to add salt to the

mixture?2. Why was it necessary to add soap to the

mixture?3. What did the DNA look like? 4. Explain how DNA is stored in the nucleus.5. Explain what happened in the final step

when you added the alcohol.6. List two reasons why it is important for

scientists to remove DNA from an organism.