Id-1b 7jan Ab Mkdu & s3 2012 Ypd

8/12/2019 Id-1b 7jan Ab Mkdu & s3 2012 Ypd

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Antibody : Structure & Function.

The recognition of Ag is the

hallmark of the specific adaptive

immune responses

CMITCR

Ag

Humoral immunityIg (Abs)

Ag

(Yoes Prijatna Dachlan, 2010)


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Electrophoretic profiles of the serum

Yoes Prijatna Dachlan, 2010


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(Stewart,2004)



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Clonal selection theory



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Ag receptor of B lymphocytes



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Schematic representation of the primary and secondary structure of a

human IgG

(Virella G., 2007)



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Four-polypeptide Structure of Immunoglobulin Monomers

(Rodney Porter, Gerald Edelman)

(Roitts Essential

Immunology, 2011)(Yoes Prijatna Dachlan, 2013)


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The structure : 4 polypeptide chains

~ 2 identical H chains

~ 2 identical L chains

3 structural units

2 identical Fab units bind Ag

The 3rd unit (Fc)

binds effector

molecules to trigger

Ag elimination

to mediate functions

such as maternal-fetal

transport

Arranged to span

(Roitts EssentialImmunology, 2011)(Yoes Prijatna Dachlan, 2013)


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(Roitts EssentialImmunology, 2011)


The neonatal receptor for IgG


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(Gregory Stewart, the Immune System,2004)


ANTIBODY SINGLE ACTION


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(Abbas, 2012) (Yoes Prijatna Dachlan, 2012)

HIV Life Cycle


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Model for neutralization of HIV by antibody (Ivan Roitt, Ess Immunol, 2011)(Yoes Prijatna Dachlan, 2012)


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Opsonization of bacteria



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S. pneumoniae

OpsonizationC3b

CRO CDN

C1q CRP SAP

Phagocytosis

Receptor for

CRP SAP

C3bRFcR

S. pneumoniae



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Opsonization of bacteria



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Antibody-dependent cellular cytotoxicity

(ADCC)



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Ig protein domain = Ig fold

(Yoes Prijatna Dachlan, 2010)(Janeway, CA., 2001)


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Antigen binding site



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Ag can bind in pocketsor groovesor on

extended surfacesin the binding sites of

Abs

(Yoes Prijatna Dachlan, 2010)(Janeway, CA., 2001)(Janeway, CA., 2001)


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(Janeway, CA., 2001)



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(Stevens, CD.,2010)



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CDRs bind antigen

CDR : Complementarity-determining region; hypervariable region


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(Infectious Disease : Shetty N, 2009)



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(Kuby, 2006)(Yoes Prijatna Dachlan, 2010)


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(Kuby, 2006)



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IgM



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(Gregory Stewart, the Immune System,2004)(Yoes Prijatna Dachlan, 2011)


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(Electron micrographs, Drs A. Feinstein & E. A. Munn)

Structural changes in IgM associated with complement activation


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IgA



(Gabriel Virella, 2007, Med Immunol, 6th ed., p55)


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IMMUNOGLOBULIN SUPERFAMILY



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(Stewart,2004)(Yoes Prijatna Dachlan, 2011)


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Interaction between B and Th


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B cell

CD28

CD40CD40L

MHC Class II*TCR

ICAM-1

ICAM-3

LFA-1

LFA-3CD2

mIg

Th cell

CD80/CD86

*CD4

A

B

C

D

E

(Yoes PrijatnaDachlan,2011

Interaction between B and Th

cells


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Signaling functions between ThB cell

interaction

A: TCR signal transduction

B: Activation and proliferation of the B cell

C: Co-stimulatory signals to the responding Tcells

D: Helps to drive B cells into cell cycle

E: Receive signals from the T cells for divisionand differentiation

(Yoes PrijatnaDachlan,2010


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Ab synthesis : IgM to IgG



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Mechanisms of Ab synthesis

Ehrlichs side chain hypothesis :is close to to the present view of clonal

selection

Dreyer & Bennett : one gene, one

polypeptidedogma untenable

Proved D & B hypothesis; somatic

recombination



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(Yoes Prijatna Dachlan, 2010) (Janeways.ImmBiol., 2008)


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(Janeways.ImmBiol., 2008)


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(Stevens, CD.,2010)



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Mechanisms for the generation of Ab

diversity

1. Multiple germline V genes

2. VJ and VDJ recombinations

3. N-nucleotide addition4. Gene conversion

5. Recombinational inaccuracies

6. Somatic point mutation7. Assorted heavy and light chain


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GENE Chromosome

H M

Light chain 22 16

Light chain 2 6

Heavy chain 14 12

Chromosomal locations of

Ig genes

in human and mouse


I t it hi


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Isotype switching


Chimeric and hybrid


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Chimeric and hybrid

monoclonal antibodies

engineered by

recombinant DNA

technology

Id-1b 7jan Ab Mkdu & s3 2012 Ypd

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