IB Cell Culture & Char

7/27/2019 IB Cell Culture & Char

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Source: IB cell culture & characteristics

1. Which of the following bacteria can grow in acidic pH?

A.Vibrio cholerae

B.Lactobacilli

C.ShigellaD.Salmonella

View AnswerWorkspaceReportDiscuss in Forum

2. Cell size and cell number can be measured usingA.hemocytometer

B.coulter counter

C.petroff-hausser counting chamberD.none of these


3. Which of the following is used for the proper maintenance and preservation of pure cultures?A.Periodic transfer to fresh media

B.Preservation by overlaying cultures with mineral oil

C.Preservation by lyophilizationD.All of the above

View AnswerWorkspaceReportDiscuss in Forum4. In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at

A.-60 to -78C

B.-20 to -30C

C.-30 to -48CD.-48 to -58C


5. The solidifying agent commonly used in preparation of media is/are

A.agarB.silica gel

C.both (a) and (b)

D.none of these

6. Which of the following chemotherapeutic agents may affect the growth ofCampylobacter jejuni?A.Vacomycin

B.Polymyxin

C.TrimethoprimD.None of these


7. To select cholera-causing bacterium, Vibrio cholerae, the pH of the medium is maintained at a pH of

A.8.5 B.8.90

C.6.79 D.3.5View AnswerWorkspaceReportDiscuss in Forum

8. For the selection of endospore-forming bacteria, a mixed culture can be heated at

A.80C for 10 minutes

B.70C for 10 minutes

C.60C for 10 minutesD.90C for 10 minutes


9. The concentration of agar to obtain semi solid media isA.1.5-20%

B.0.5% or less

C.>10%D.>10%but


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11. Mac-Conkey medium is an example of

A.transport mediumB.enrichment medium

C.differential medium

D.all of theseView AnswerWorkspaceReportDiscuss in Forum

12.Pseudomonas aeruginosa forms a blue water soluble pigment called

A.pyocyanin B.chlororaphin

C.pyoverdin D.-caroteneView AnswerWorkspaceReportDiscuss in Forum

13. Agar is used for solidifying culture media becauseA.it does not affect by the growth of bacteria

B.it does not add to the nutritive properties of the medium

C.the melting and solidifying points of agar solution are not the same

D.all of the aboveView AnswerWorkspaceReportDiscuss in Forum

14. For the selection of lactobacilli present in cheddar cheese, the pH of the medium is maintained atA.6.35 B.5.35

C.4.35 D.5.75


15. A disease producing species occurring in a mixed culture can often be selected on the basis of itsA.pathogenic properties

B.special carbon source

C.special nitrogen sourceD.none of these

16. Important example(s) of enriched medium/media is/ are

A.Loeffler's serum slope

B.Bordet-gengou medium

C.Blood agarD.All of these


17. Cell counting can be carried out byA.direct microscopic count using petroffhansser counting chamber

B.plate counting

C.membrane filter countD.all of the above


18. Which of the following techniques may be performed quantitatively to determine the number of bacteria ofa particular type?

A.Pour plateB.Spread plateC.Both (a) and (b)

D.Streak plate technique


19. Intestinal bacteria can grow in the presence of __________ whereas nonintestinal bacteria are usually

inhibited.

A.bile salts

B.low concentration of various dyesC.sugars

D.low levels of nitrogen


20. Which of the following problem makes it impossible to satisfy all of Koch's postulates?

A.Microorganism causes serious symptoms in humansB.Microorganism cannot be isolated in pure culture

C.Species of microorganism is in question

D.Genes from the microorganism cannot be amplified by PCR

21. A medium containing crystal violet dye plus sodium deoxycholate will allow

A.gram (-)ve intestinal bacteria to grow
http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-190http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-215http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-196http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-207http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-192http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-186http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-212http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-203http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-190http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-215http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-196http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-207http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-192http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-186http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-212http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-203


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B.gram (+)ve intestinal bacteria to grow

C.aquatic bacteria to growD.none of these


22. Which of the following bacteria can grow in alkaline pH?A.Shigella

B.Lactobacilli

C.Salmonella

D.Vibrio choleraeView AnswerWorkspaceReportDiscuss in Forum

23. All bacteria that inhabit the human body areA.heterotrophs B.chemolithotrophs

C.autotrophs D.phototroph


24. When a substance is added to a liquid medium which inhibits the growth of unwanted bacteria and favors

the growth of wanted bacteria, it is known as

A.differential mediumB.enriched medium

C.enrichment medium

D.selective mediumView AnswerWorkspaceReportDiscuss in Forum

25. Which of the following methods is most likely to be quantitative?

A.Dilution and plating

B.Gram stainingC.Wet mount

D.None of these

26. Which of the following hint(s) suggest/s that a given specimen is likely to contain anaerobic bacteria?

A.Gas in specimen

B.Foul odourC.Presence of sulphur granules

D.All of theseView AnswerWorkspaceReportDiscuss in Forum

27. Viable plate count can be obtained using

A.Spread plate method

B.Pour plate methodC.both (a) and (b)

D.Hemocytometer


28. In the pour plate method, the mixed culture is diluted directly in tubes of

A.liquid agar mediumB.sterile liquid usually waterC.both (a) and (b)

D.none of these


29. Treponema species from human oral cavity can be selected by using

A.pH of the medium

B.incubation temperature

C.cell size and motilityD.all of these


30. When a substance is added to a solid medium which inhibits the growth of unwanted bacteria but permits

the growth of wanted bacteria, it is known as

A.differential mediumB.enriched medium

C.enrichment medium

D.selective medium

31. Peptone water and nutrient broth are

A.selective media
http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-204http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-199http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-201http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-195http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-202http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-188http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-211http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-209http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-204http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-199http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-201http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-195http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-202http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-188http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-211http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-209


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B.enriched media

C.basal mediaD.none of these


32. Stuart's transport medium is used for transport of specimen containingA.Neisseria gonorrhoeae

B.Salmonella

C.Vibrio cholerae

D.ShigellaView AnswerWorkspaceReportDiscuss in Forum

33. Plate counts are often reported asA.cfu

B.cfu/ml

C.Both (a) and (b)

D.thousands/mlView AnswerWorkspaceReportDiscuss in Forum

34. The medium which allows the growth of more than one microorganisms of interest but withmorphologically distinguishable colonies is known as

A.selective medium

B.enrichment mediumC.differential medium

D.none of these


35. Selective media facilitate growth of only one kind of organism. Saboraud medium is used to selectivelyisolate

A.coliform bacteria

B.gram positive bacteriaC.yeasts

D.acid fast organisms

*****until #35 only, nothing follows*****
http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-191http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-197http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-187http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-193http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-191http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-197http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-187http://www.indiabix.com/microbiology/cell-cultures-and-characteristics/discussion-193

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