EXCRETION. Homeostasis To understand excretion, we need to understand homeostasis –
(i)1: Screen to identify new key players linking iron homeostasis and transport
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(i)1: Screen to identify new key players linking iron homeostasis and transport. Fe homeostasis gene families from peach. - PowerPoint PPT Presentation
Transcript of (i)1: Screen to identify new key players linking iron homeostasis and transport
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ZIP, OPT, YSL, NRAMP, FRO, FER, NAS, IREG8 families
- 1.013 metabolites Fiehn's database
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IEF-SDS/PAGELabel free shotgun proteomicsBlue native (PAGE-SDS/PAGE)iTraqDIGEImage analysisICAT
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5 batches (biological replicates) with all treatments1 gel (7 cm BioRad) per biological replicate (5 biol. rep. x 3 treatments, 15 geles in total)
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249 spots, 54 unambiguous id (+44)
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FeZn
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Resupply
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Gutierrez-Carbonell et al. In preparation
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search
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63 spots62 identified
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252 metabolites consistently present(in 80% of the samples -in 5 samples out of 6- in at least one class)
knownunknown104148
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10 known metabolites(5 increase, 5 decrease)
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Root phenolic compounds increasing with Fe deficiencyPDR3 ortholog in P. persica: ppa000248m
PDR9 orthologs in P. persica ppa000249m and ppa023381m (ppa017338m, ppa000229m with homologies)
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1 gel (7 cm BioRad) per biological replicate (2 batches x 3 reps x 3 treatments, 18 gels in total)
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El-Jendoubi et al. 2012 PhD Thesis Growth chamber Field
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uptake from soil componentsX
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-Fe(III)-carboxylatesFe(III)-malate-A FlavinRodrguez-Celma et al. 2011 Plant Cell Physiol 52, 2173-2189-A Fe(III) complex derived from natural polymers of humic and lignine natureMellisho et al. 2011 J Sci Food Agric 91, 2132-2139Fuentes et al. J Sci Food Agric, in pressFe(III)-citrateFoliar fertilization candidatesSoil fertilization candidatesTo be started in June 1, 2012
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