http://tums.ac.ir/faculties/emaneini

Mohammad  Emaneini,

PhD

Assistant Professor in Microbiology

Department of Microbiology

School of Medicine

Tehran University of Medical Sciences

emaneini@tums.ac.ir

Emaneini M. PhD.

Transformation A DNA fragments/plasmids of donor bacteria

Taken up & incorporated into the genome of recipient cell

Natural competence

Streptococcus pneumoniae, Haemophilus influenzae, Neisseria

gonorrhoea

Induced competence

Cold CaCl2 & heat shock: E. coli

Protoplast: Bacillus, Streptomyces

Electroporation: a brief pulse of high-voltage electricity

Emaneini M. PhD.

TransductionTransfer of DNA by Bacteriophage via Lytic & Lysogenic cycle

Generalized transduction Specialised transduction

Emaneini M. PhD.

Discovered 1946 Lederberg &

Tatum

Donor:

Containing conjugative plasmid

(F+)

Recipient:

Bacterial receiving the plasmid

(F-)

Conjugation

High-frequency recombination (Hfr)