H&S Reference Summary of the Assessment / materials used

965 precipitation of asphaltenes from heavy crude and the preparation of metal adducts

964 precipitation of asphaltenes from heavy crude and the preparation of metal adducts

963 reaction of raney nickel with hydrogen peroxide 962 use of liquid nitrogen to powder fruits and vegetables 961 use of 0.1M sodium azide stock solution to remove antibodies from

nitrocellulose membranes 960 preparation of a diet containing 0.005% (or similar concentration) of

Perfluorodecanoic acid (PFDA) and administration to rats 959 using liquid nitrogen for demonstrations 958 sol-gel preparation 957 preparation of Mn exchanged kaolinite, laponite and montmorillonite-

based catalysts 956 synthesis and preparation of alumina and zinc-oxide by co-precipitation

from their mixed nitrate solutions 955 preparation of manganese-cobalt spinel 954 preparation of Nickel on charcoal powder 953 distillation of N,N,N',N'-Tetramethylhexane-1,6,diamine (TMHDA) 952 analysis of nickel from potassium hydroxide using a solvent extraction

method 951 long term testing of fuel cell electrodes 950 addition of potassium carbonate to koh and water to determine

concentrations in koh being used in a fuel cell half cell before and after testing conditions

949 to electroplate silver onto a carbon surface using a silver rod and silver nitrate in water

948 making of calibration standards to amnalyse the amount of carbonate dissolved in the koh

947 acid base titrations to determine ion exchange capacity of different materials

946 preparation of tween-80-expanded and brij-series-expanded molybdenum trioxide

945 preparation of tween-80-expanded and brij-series-expanded vanadium oxide

944 preparation of tween-series-expanded molybdenum trioxide 943 phenethylthiourea preparation for lc/ms analysis 942 preparation of au/c/alkaline electrodes for use with alkaline membrane 941 preparation of tween-series-expanded vanadium oxide 940 staining agarose gels by gelred nucleic acid gel stain 939 drying bacterial samples for sem analysis 938 preparation of tween-60 expanded vanadium oxide 936 to electroplate silver onto a carbon surface using a silver rod and silver

nitrate in water 935 analysis of ti-doped apatite materials for their ability to decompose

acetaldehyde in dark, natural and uv-lit conditions.

934 infection of tetrahymena pyriformis with mycobacterium bovis 933 preparation of pt/c/alkaline electrodes for use with solvay membrane 932 addition of potassium carbonate to koh and water to determine

concentrations in koh being used in a fuel cell half cell before and after testing conditions

931 acid base titrations to determine ion exchange capacity of different materials

930 cadmium chloride to dose live mammalian cells 929 grafting of kaolinite clay 928 influence of lead concentration on iron uptake by caco-2 cells 927 preparation of magnetic cellulose sponges containing charcoal

adsorbents 926 fatty acid analysis by gc. transesterification of oil using sodium methylate

and mtbe. 925 determination of total carbohydrate by colorimetry, using 5% phenol and

h2so4 924 tert-butyl methyl ether (mtbe) for tranesterification of lipids, fatty acid

analysis by gc. 923 analysis of arsenic using leucocrystal violet uv/vis assay 922 analysis of arsenic using rhodamine-b uv/vis assay 921 analysis of arsenic using azure b uv/vis assay 920 reaction of n-acetyl-s-(2,3,5-trio-benzoyl-d-ribofuranosyl)-l-cysteine,

workup, tlc and 1h nmr. 919 risk assessment of a replication-competent reovirus (reolysin tm) vector

for an in-vivo study. 918 making rossman's fluid and subsequent use to fix liver tissue. 917 risk assessment of a replication-competent reovirus (reolysin tm) vector

for an in-vivo study. 916 to produce an etfe-alkaline anion exchange membrane (etfe-aaem) by

converting etfe-g-(poly)vbc to etfe-g-(poly)vinylbenzyltrimethylammonium chloride

915 solgel synthesis of hydroxyapatite systems 914 synthesis of silver doped hydroxyapatite 913 the precipitation of lanthanum compounds 912 heating of a polymer sheet in koh and water to see any changes 911 digestions 1) wet ashing (kjedahl tubes, waterbath and squat beakers) 2)

dry ashing (muffle furnace) 910 synthesis of proton conducting pval/h3po2/h2o electrolyte membranes 909 barium chloride in detection of sulphate (4500-so4 e. turbidimetric

method. 1998. 908 to produce an etfe-alkaline anion exchange membrane (etfe-aaem) by

converting etfe-g-(poly)vbc to etfe-g-(poly)vinylbenzyltrimethylammonium chloride

907 long term testing of fuel cell electrodes 906 2-bromoethanol in ethanol 905 analysis of nickel from potassium hydroxide using a solvent extraction

method 904 preparation of polyurethane foam containing charcoal and iron filings 903 sorption of phenanthrene by charcoal 902 grafting of vinylbenzene chloride (vbc) onto electron beam activated etfe

film 901 simulated gastrointestinal digestion of soy and fish proteins by pepsin and

pancreatine and determination of the angiotensin converting enzyme (ace) activity of the derived peptides

900 synthesis of surfactant expanded mn02 899 synthesis of surfactant expanded vo2 898 synthesis of vo2 nanotubes 897 preparation of phosphoranilidates from alcohols 896 preparation of phosphoranilidates from alcohols 895 arsenic by molybdenum blue method for uv/vis detection 894 astm method d2972-03: standard test method for arsenic in water by

uv/vis 893 mycobacterium tuberculosis (28, 28a, 29ax02) 892 ethidium bromide solution used for staining nucleic acids (23ax02) 891 chloroform used in genomic dna extraction (23ax02) 890 tween 80, used in culture media (23ax02) 889 working in hplc (dan lab) and probably in 29ay01 888 nitric acid for use in digestions/sample dilutions/cleaning 887 analysis of boron using azomethine uv/vis assay -12az03 886 digestion of samples containing trace elements 28az03 885 staining of lipids in cultured cells with oil red o 884 culture of adherent cell lines (e.g. cos-7, at3-1), including use of liquid

nitrogen and virkon 883 cell culture of 3t3-l1 fibroblast cell line and differentiation into adipocytes 882 preparation of a nafion mea using pt and pt/ru electrodes 881 membrane immobilisation of glucose oxidase, membrane casting,

voltammetry 880 synthesis of poly (ethylene glycol) dimethacrylate 879 analysis of boron using azomethine uv/vis assay 878 use of nitric acid in digestion 877 digestion of supplements in squat beakers 876 use of ionic liquids for separations and analysis 875 making up small molecule standards for accurate mass lc-ms 874 operation and maintenance of ilab 650 873 automated analysis of creatinine, triglycerides, glucose (oxidase) and

cholesterol using instrumentation laboratory (il) kits, calibrators and quality control (qc)

872 preparation of blood samples taken for f2 isoprostane analysis 871 measurement of ldl and hdl subclasses using iodixanol density gradient

ultracentrifugation 870 automated analysis of glucose, triacylglyerol and total cholesterol using

randox/ alfa wasserman test kits, calibrators and quality controls. 869 automated analysis of apolipoproteins, ldl cholesterol and hdl cholesterol

using randox/alfa wasserman test kits, calibrators and quality controls. 868 use of dry ice (frozen co2) as cooling aid transportation 867 isolation of human plasma and serum by low speed ultracentrifugation 866 making up standards for gc and gc-ms 865 long term testing of fuel cell electrodes

864 sodium thioglycollate (incorporated into growth media) 863 stimulation of gonadotroph cells with [des-gly10, d-ala6]-gnrh ethylamide 862 herbs extraction (volatile oil and fixed oil) using different solvents 861 pot trial using soils contaminated with heavy metals, including aresenic,

lead, cadmium, zinc and copper. 860 synthesis of pigments 859 low temperature synthesis of apatites, coprecipitation of solutions with

refluxing 858 inhibition of transcription using actinomycin d 857 barium chloride and barium sulfate 856 using resazurin to monitor the redox potential in anaerobic media 855 formation of aluminium keggin ion and subsequent insertion into layered

clays to form pillared species 854 methanol-sunflower oil interaction with koh catalyst 853 drying solvent by refluxing over calcium hydride 852 synthesis of hydroxyapatite 851 synthesis of hydroxyapatite 2 850 chn analysis 849 making up matrices for maldi-mass spec 848 preparation and use of carrez a (zinc sulfate heptahydrate) and carrez b

(potassium hexacyanoferrate(ii) trihydrate) reagents, which are used for protein precipitation and clarification of samples destined for analysis by hplc or lc-ms(-ms).

847 hand gun residue testing 846 synthesis of mno2 nanotubes (permanganate route) 845 synthesis of g-mno2 nanotubes (permanganate route) 844 synthesis of mno2 nanotubes 843 synthesis of moo3 nanotubes 842 use of chn gases in 25az03 841 the preparation and testing of pt/c/alkaline electrodes 838 preparation of bromo resorcarene 837 making up standard solutions for calibration and specification checking of

mass spectrometers (new user) 836 antibiotic selection of transfected cells 835 cleaning and reinstallation of lct mass spec sample cone. 12ay03 and

13ay03 833 solid phase extraction and hplc analysis of drugs/pesticides 832 icp-ms analysis of lead and cadmium (nhs - 36ay04) 831 heat killing of m. tuberculosis/m. bovis for removal from the cl3 laboratory 830 preparation of diets containing 2500ppm (or similar concentration) of

clofibric acid and administration to rats 829 preparation of diet containing 1000ppm (or similar concentration) of

nicotinic acid (niacin) and administration to rats 828 growth and maintemnance of acanthamoeba castellanii 827 synthesis of calix[4] pyrrole derivative 826 making up standard solutions for calibration and specification checking of

mass spectrometers 825 dismantling, cleaning and reassembly of lct mass spectrometer source

824 immunisation of mice with neisseria meningitidis 823 acid and base baths to clean glassware and utensils 822 formalin disinfection of small samples of mycobacterium

tuberculosis/bovis/bcg pateur 821 continuous and batch cultures of mycobacterium tuberculosis/bovis/bcg

pateur 817 cress pytotoxicity trial for diesel contaminated soil 816 pot trial using contaminated soil from a site contaminated with metals 815 handling and mixing of contaminated soils 814 growth of campylobacter jejuni in minimal media with iron sources 813 growth of campylobacter jejuni in minimal media with carbon sources 812 staphylococcus aureus (growth and manipulation in the laboratory 811 listeria monocytogenes (growth and manipulation in the laboratory) 810 vibrio parahaemolyticus (growth and manipulation in the laboratory 809 salmonella sp. (growth and manipulation in the laboratory) 808 enterobacter sakazakii (growth and manipulation in the laboratory) 807 use of thimerosal (also called thiomersal) as an antibacterial agent 806 viscosity experiments using acid tar and charcoal 805 soxhlet extraction: extracting pyrene and phenanthrene from charcoal -

background compounds 804 growth of shaken liquid cultures of mycobacteria 803 preparing gc-ms standards: pahs 802 preparing gc-ms standards: diesel 801 isolating polycyclic aramatic hydrocarbon (pah) degrading bacteria from

innoculated charcoal 800 adding polycyclic aromatic hydrocarbons (pahs) and minerals to charcoal

and innoculating it with pah degrading bacteria 799 Spraying soil / charcoal isolation plates with PAHs dissolved in ethyl ether 798 Establishing a large community of diesel (hydrocarbon) degrading

bacteria in charcoal 797 Forensic Practical "Bloodstains on the ground" 796 Forensic Chemistry Practical "A Pool of Evidence" 795 Forensic Chemistry Practical "White Powders" 794 Preparation of Delipidated Foetal Bovine Serum 793 Solutions to be reacted with Cu2+ 792 Measurement of hydrogen peroxide using the o-dianisidine colorimetric

method 791 General risk assessment for the use of atomic absorption spectroscopy 790 Preparation of diets containing ciprofibrate 789 3-morpholinosydnonimine in a disc diffusion assay against Campylobacter

jejuni 788 Cadmium chloride in a disc diffusion assay against Campylobacter jejuni 787 Chitinase assay of bacteria using 1% chitin TSA plates 786 Manipulaion of Plesiomonas shigelloides 785 Measurement of the rate of entry of metal ions into the skin 784 Analysis of diesel emmitted particles in water as a suspension 783 Treatment of cells in culture with rapamycin

782 Minimum inhibitory concentrations using uric acid with Campylobacter jejuni and disc diffusion assays with uric acid and C. jejuni

781 Rapid screening method for the quantition of bacterial cell lipids from whole cells

780 Entrapment of sodium tetraphenylborate in PVC membrane 779 DRAQ5 used for the cromatin staining of cells cultured on a coverslip or a

suspension 778 Trichloroethyl chloroformate as a hydroxyl protecting group 777 para-toluene sulphonic acid (hydrate) 776 Molecular Seive (dust) 775 Oxalyl chloride for carboxylic acid chloride generation 774 Hydroxylated and/or methoxylated cinnamic acids for use in synthesis 773 4-dimethylaminopyridine 772 Desiccation of tissue sections 771 6-phosphogluconate 770 clofibric acid 769 milk practical 768 acetamide 767 oxidation damage to proteins 766 insect cell tissue culture 765 autoradiography 764 Legionella pneumophilia 763 RNAprotect 762 cell cytotoxicity assays 760 nitric oxide measurement 759 carbohydrate assay 758 electrochemical synthesis of quantum dots 757 anti-Campylobacter assay 756 enzymatic assay 755 glycerol assay 754 isolated tissue work using organ baths 753 effects of R- & S- enantioners on rats 752 coating the quartz crystal 751 oxidative stress assay for Campylobacter 750 analysis of selenium in serum 749 spray disinfectant for cleaning safety cabinets 748 preparation of microarray slides 747 interactions of polyaromatic hydrocarbons 746 cell staining for oxidative bursts 745 polycyclic aromatic carbon interactions 744 extraction of primate nucleic acids 743 degradation ofo-nitrophenol by fungi 742 calibration of MALDI-TOF 741 molecularly imprinted polymers 740 3-methyl-2-benzothiazolinone hydrazone 739 clavulanic acid assay 738 spiking of sand systems

737 quinone derivitisation 736 affinity electrophoresis 735 COX-2 inhibitors 734 decolourising culture supernatant 733 amino/organic acids for Streptomyces 732 administration of BHT to rats 731 TLC 730 HPLC standards 729 Barry degradation 728 simple phenolic & polyphenolic compounds 727 formaldehyde assay 726 quality assessment standards 725 measurement of superoxide production 724 piperazine diacrylamide for 2D PAGE 723 chromogenic substrates for sulfatase 722 benzyl mercaptan 721 nitrosylating agent 720 methylation 719 tert-butyl ether 718 immunogen 717 receptor binding 716 extraction of RNA from shellfish samples 715 glucose assay kit 714 pyrrolidinedithiocarbamate 713 PGE2 assay 712 western blotting 711 Proteinase K for DNA extraction 710 incubation of cells with cadmium chloride 709 hydrogen peroxide determination using QCM 708 detection of organophosphates with the QCM 707 section staining for copper with Timm's stain 706 Greiss assay for nitrate/nitrite determination 705 culture media additives 704 detection of hydrogen peroxide 703 electropolymerised polymer membrane 702 pyrithione-mediated Zn uptake 701 LDL-cholesterol receptor assay 700 phosphorus pentachloride 699 enzymatic conversion using the QCM 698 optimisation of membrane composition 697 catabolism of metalloferrocyanides 696 identification of aldehydes 695 phosphorylation 694 culture media additives 693 phenol reactions using the QCM 692 reverse connection polymerase chain reaction 691 coating internal surfaces of bioreactor

690 intracellular Caz+ measurement 689 addition to soil bacterial cultures 688 n-acetylcysteine/cinnamon reaction 687 Carnoy's fluid - fixative 686 radical generator 685 anthocyanins & anthocyanidins 684 purification of myoglobin 683 Sirius red staining for collagen 682 Acanthamoeba, Myco bovis 681 cleaning the quartz crystal 680 derivitisation of adenosine 679 effect of cadmium on degradation of PAHs 678 biological buffer 677 iron chelating 676 determination of cholesterol & creatinine 675 polymer films to modify surface of quartz crystal 674 inhibition of free radical reactions 673 iron binding capacity assay kit 672 spin coating the quartz crystal 671 1-octanesulphonic acid 670 extraction & analysis of ascorbic acid 669 culture media antibiotic 668 tissue culture additives 667 antimicrobial activity assessment 666 casting polydimethylsiloxane films 665 thioctic acid & pyridoxine 664 manganese chloride 663 monomeric phenolic compounds 662 ammonium bicarbonate 661 adenosine release studies 660 biodegredation of benzo[a]pyrene 659 isocitrate liase assay 658 vitamin E analysis 657 solubilisation buffer for protein extraction 656 transfection of plasmid DNA into cells 655 a-glycerophosphate dehydrogenase assay 653 K+ & Cl- channels in rat cells 652 measurement of lipid hydroperoxides 651 penicillamine 650 antioxidant assay 649 calixarenes & silica to modify quartz crystal 648 preparation of solution 647 HPLC standards 646 cleaning the quartz crystal 645 immobilisation of RNA/DNA probe 644 culture media additives 643 measurement of total antioxidant activity

642 effects of second messengers on intestinal nutrient uptake 642 staining cells 641 studying anti-tumour activity using cell lines 640 neurochemical analysis of neuronal function 639 testosterone hydroxylase assay 638 extraction of phenanthrene from chicken manure 637 dilution of phenanthrene 636 neurochemical analysis of glial cell function 635 inhibition of protease activity 634 chemicals used in 33AY04 633 production of indophenol dye 632 carcinogens used in 33AY04 631 harmful/irritant chemicals used in 33AY04 630 decontamination of soil containing BCG 629 mutagenesis assay 628 removal of zinc from bovine serum 627 extraction of anthocyanins from fruit 626 iron transport assay 625 polyacrylamide gel electrophoresis 624 RNA isolation 623 thymidine uptake by mammalian cells 622 cell culture 621 cell metabolism studies 620 isolated tissue work using organ baths 619 anaesthesia 618 tissue culture additives 617 aluminium, cobalt,, chromium, copper, gold, iron, lead, manganese,

magnesium, selenium & zinc standard solutions 616 nickel standard solution 615 mercury standard solution 614 cadmium standard solution 613 arsenic standard solution 612 Use of bovine serum & horse blood 611 Protein purification, immunoprecipitation 610 Iodination of proteins 609 Electrophoresis, radioimmunoassay 608 Selective media 607 Antioxidant assessment 606 mRNA isolation 605 AJ buffer 604 Gas chromatography 603 Assay for renin inhibitors 602 Human tumour biopsies 601 Culture media additives 600 Effect of chemicals 568 CHAPS-detergent for protein cells 566 RNA extraction from mycrobacterium tuberculousis need to be ribolysed

564 gel retardation with in-gel DNA foutprinting 563 extraction of nuclear extraces 561 cytotoxicity studies with NHEK cells 560 cleaning of glass sample by sonication in methanol 559 use in tissue culture assay 558 use of glycerol for storage of bacterial cultures 556 use of diethylene glycol as a carbon source for bacteria in mineral water 554 dimethylsulphoxide (DMSO) 553 chloroform, hexane, boroutrifluoride/methanol 552 use of isophthalic acid as a carbon source for the growth of

microorganisms 549 terephthalic acid 548 biodegranation of cnude oil 547 substractive hybridisation 546 polymerase chain reaction 545 glutamine transaminase k assay 544 DNA extraction from agarose/bacterium using KITS 543 carboxylation of sceleroglucan using sodium periodate and sodium

chlorite and acetic acid 542 use of 3 amino 1,2,3 triazole in culture media 541 investigation into the use of pahs as a sole carbon source for

mycobacteria 540 paraformaldehyde 539 use of soybean oil in microbiological media 538 l-chloro-2,4 dinitrobenzene use to detect fusion proteins 537 nelson assay for soluble sugars 536 stimulation of cells with staphylococcus enterotoxin from staphylococcus

aureus 534 rhodanile blue cell culture staining 533 anti fude 532 digestion of liunocellulosic material 531 removal biofilm from mineral water bottles using 1% sodium

pyrophosphate 529 mutagenic treatment of steptomycetes with ethyl methane sulphoate 528 use of sodium azide 527 preparation of serum containing medium (keratinocyte medium) for

human skin cell culture 526 category 2 viruses 525 poly-l-lycsine use as fixative for bacteria/cells/tissue prior to staining 524 use of aprotinin as protease inhibitar 523 application of nitrapynin (2-chloro-6(trichloromethyl) pynidine) to soil

samples 521 mistogen induced cell proliferation assay 520 diatomaceous earth 519 proteases-brotelain, elastase, endoproteinases glu-c, papain, l-

chymotrypsin 518 use of cl-nerf for ph determination in tissue 517 sonication of mycobacterium bovis bcg using sound chamber

516 vancomycin-hcl 515 tunicamycin 514 penicillin-G potassium 513 cinnamic aldehyde 512 bacitracin 511 use of virkon as a virucidal disinfectant 509 preparation of 6% L-naphthol in ethanol to assay diacetyl in yoghurt 508 n-decyl aldehyde used as substrate 506 digestion of soil and plant material 505 use of brays nitrate-nitrite powder in most probable number assay for

nitrogen cycle microorganisms 503 cellulose type 20 502 reduction of carboxyls on xanthan using 9-barabicyllo 501 ammonium metavanadate 500 antimony, potassium tartrate 499 treatment of bacteria cells 499 methyl viologen 498 reduction of carboxylin acid in xantan gum using barane-tetrahydrofuran 497 tetrahydrofuran 496 use of Rnase-away 494 making up vitamin supplements for gnotobiotic rats 493 breeding experimentation with rats 492 human decidnal tissue 491 superoxide dismutase 490 titration procedure for catalase assay 489 trace methal additive for pining water medium 487 polyvinylpolypyrrolidone-cell culture 486 production and extraction of patulin 484 nystatin 483 handling hazard group 2 micro-organisms 481 opening of freeze-dried ampoules-containg micro-organisms 480 modification of sulphydryl group using iodoacetic acid 479 nitrilotriacetic acid 478 polysaccharide extracellular 477 production of Ag/Agci flectrodes by electrolysis 476 determination of pyruvyl 475 boiling of boric acid solution 474 DNA-purification resiri 473 isolation of human plasma lipoproteins by preparative ultracentifugation 472 chemical mutagenasis of saccharopolyspora erythraea using methane-

sulphonic acid ethyl ester 471 ciprofloxacin 470 norfloxacin 469 deacetylation of xanthan and related polymers 468 fixation of phanti tissue 467 phasphorus elimation using sodium metabisuphite 466 listenia monocytogenes

465 uranyl acetate 464 titration for chloride using potassium thiolynate 463 treatment of listeria monotycogenes with phospodiesterase l type lV 462 extraction and methylation of bacterial fatty acids 461 nickel sulphate 458 wease assay 456 for producing storage stock solutions of xgal (carbohydrate analogue) 455 n-octane 454 cyanida detoxification by fungi 454 rozoshere remediation of toxins in soil 453 weighing powdered media 451 purigication of immunoglobulin from tissue culture supernatant 450 trolox 449 modificiation of lipid composision in bacterial cell using myristic acid 448 iodization of plastic bamphes to reduce microbial cononization of inetrior

surfaces 446 acetyl determination 445 used as ph indicator of totrated solution 444 use of sodium borohydride as a meducing agent in small scale 443 handling citrinin as a chromatolographic or extraxtion standard 442 tris (hydroxymethyl) 441 magnesium oxide 440 used as standart for calibrate the hygrography 439 tergitol 438 measurement of heart rate and blood pressure using mercury

sphygmomanometers 435 sulphuric acid used to titrate extract solutions for ammonium

determination in soil 433 silver sulphate-urease assay 432 adenosine-antyfoamaemulsion, edta acit ATP content in the soil 428 the effect of pancreatin, trypsin, pronase on the M.I.C of nisin against

listeria monocytogenes 427 the effect of chymotripsin on the n.i.c of nisin against listeria

monocytogenes 426 galvinoxyl 425 minocycline 424 tetracycline 423 removed of layer protein using lithium chloride and effect on M.I.C of nisin

ageinst l.monocytogenes 422 pyruvate determination 421 hygromycin 420 RNAzol-b 419 used as TCL stain 418 preparation of samples for transmission electron microscopy 417 patulin 416 propidium ioade staining of cells 415 effects of compounds on the replication of cells 414 assay to detect phagocytosis/cytotoxicity

413 determination of non-specific esterase acting in cells 412 tissue culture and enumeration of cells 411 formaldehyde solution 410 DNA extraction from bacterial cells 409 ammonium iron citrate 408 using potassium cyanide to inhibit bacteria 407 effect of potassium metabizulphite on zygosaccharomyces bailli 406 determination of copper concentration in water samples with sodium

diethyldithiocarbamate 404 klorextra 402 negative staining with 1% tungstophosphoric acid 401 rhodamine 123 400 mixed lymychocyte culture 397 electropolishing of coppre electrodes 396 dapi-cell staining 394 cyanoditoly tetrazolium chloride or ctc 392 iso-nicotinic acid hydrazide 391 4-chloro-napthol-colour substrate for horse darish peroxidase 390 sbs 218 practical microbial physiology,ecology 389 dichloran-glycerol agar base containing botran 2,6-cichloro-4-nitro-aniline 388 extraction of 5-layer proteins with 5m guanidinium hydrochloride 387 estimation of mic of p.pancimobilis with h2o2 using a ctalase inhibitor 3-

amino-1,2,4-triazole 386 extraction of bacteria pigment using acetone 385 superoxide dismutase 384 catalase 383 mycophenolic acid 382 amonopterin 381 the use of fatty acid and hydrocarbons as carbon sources for

thraustochytrids 380 transformation of yeast using lithium acetate and single-standed carrier

DNA, by heat shock 379 melachite green solution for spore stain 378 growth of influenza virus in emryonated chicken 376 rose gittlieb orocess for fat determination in milk 372 handling potassium cyanide 371 handling methyl cellosolve 367 scintillation fluid (optiplasa safe) 366 nucleon chicago solubilizes (NCS)-tissue solubilizer 364 determination of heme by fluorescence techniques 361 fluorescein diacetate-for fluorescent microscope 360 use of anisaldehyde as visualisasing agent for TLC plates 356 nisin elisa-the use 3-3-5-5- tetramethylbenzidine di HCL 350 INT 349 anisole-side chain deprotection,peptide synthesis 347 use of hydroethidine for flow cytometric analysis of reactive oxygen

metabolites in mononnuclear cells

346 methyl cellulose for cryopreservation of serum free cell cultures 345 dichloroacetic acid-supplememntation of cell culture media 344 supplementation of cell culture media 344 use of 2,7-dichlorofluoressin diacetate for flow citometric analysis of

reactive oxygen matabolites in mononuclear cells 343 l-malic acid 342 substrate for elisa 339 drug sensitisation of cell line;to induce the growth of tk-cells 338 cell mutation,skaning nuclei, cell cycli analysis using the falscan 337 dimethyl sulphoxide used to protect cells when frozen down 336 use of erythromycin 335 polyethylene glycol 334 tetraethyl silicate 332 to eamine effects on penicillin production 331 electrical detection of listeria 328 use of API 20E bacterial identification kits 328 API reagent 327 preparation of Ig G fraetion from sheep serum 326 final year food microbiology practical 325 2nd year food microbiology practicals 321 arginine-glucose medium 320 processing biological materials for electron microscope 320 industrial microbiology 319 tetrazolium salt 318 cobalt chloride 317 ammonium nitrate 316 3.5 di-nihosalycilic acid-use in glucose assay 315 practical physiology and microbiology 314 practical bacteriology 311 experiments using endotoxin 309 horseradish peroxidase 308 small scale production of printed circuit boards 307 acetaldehyde 306 diphenylamine-DNA assay 304 DNA dipstick test 303 handling concanavalin-a, con a, or cananalia ensiformis lectins 300 buthanol,pyridine, water solvent for paper chromatography 299 quantitative detetrmination of uronic acids 4-hydroxybiphenol 295 sodium aurothiomalate 294 5-bromo-4-chloro-3-indolyl beta-d-galactopyranoside 293 8-hydroxyquinoline 292 assaying hydrogen peroxide concentrationusing titanium sulphate 291 assaying superoxides in oil using nitrotetrazolium blue 290 use of sodium borohydride 289 use of n-n-dicyclohexylcarbodi-imide as inhibitor of ATPase activity 288 trypan blue solution 286 respiration of transformed roots

285 extraction of degraded hydrocarbons from culture media 284 trifluoroacetic acid 282 alpha-naphthylacetate- for staining white blood cells 281 staining plant material-toluidine blue 280 preparation of breed smears in food microbiology practical classes 274 di isopropylethylamine 269 experiments using stainless sted powder (maximum diameter) 268 lowry protein assay-folin and ciocalteus reagent 267 final year prosect-metabolic inhibition of streptomyles aureofaciens 266 final year prosect-metabolic inhibition of s.aureofaciens 265 final year prosect-metabolic inhibition of streptomyles 264 pthophosphoric acid 263 handling (weighting,diluting, dispensing) 262 handling (weighting,diluting, dispensing) 261 handling (weighting,diluting, dispensing) 258 diaminobenzydine (DAB) 257 use of commercial amylase preparations to saccharify starch 256 silica (silicon dioxide) in DNA and RNA extraction 255 determination of lactate by enzymatic oxidation of nicotinamide adenine

dinucleatide (NADH) 254 determionation of glutamine and ammonia concentration 253 enzyme linked immunobsorbent assay for the determination of

monoclonal antibody concentration in hybridoma culture supernatant 252 rose bengal chloramphenicol agar base 251 dichloran rose bengal chloramphenicol agar base 250 pristane 249 b-propridlactone 247 staining slides- teaching and research labs 246 periodic acid 245 hydrogen peroxide 244 citroclear for histological use as a safes alternative to xylene 243 protease fungal type XI 242 polyoxyethylene 20 cetyl ether 241 isophropy-bata-d-thiogalactoside (IPTG) 240 y-methacryloxypropyl-trimethoxysilane 239 mops free acid-buffer used in denaturing formamide gel for RNA 238 diethyl pyrocarbonate 237 adenosine tri phosphate 235 zinc sulphate 234 manganese sulphate 4-hydrate (manganous sulphate) 232 copper sulphate 5-hydrate (cupric sulphate) 231 magnesium sulphate-7-hydrate 230 dimethyldichlorosilane-used for coating plates fir PAGE electrophoresis 228 ampicillin-antibiotic selection of bacterial colonies 227 trizma-used to make buffery solution 226 temed 225 potassium acetate

224 ribonuclease C from human plasma-degradation of RNA 222 spermidine 221 dl-dithiothreitol-reducing agent 220 isoamyl alcohol (isopentyl alcohol 3-methyl-l-butanol) 219 deoxychloric acid 218 ethylenegrycol-bis-amino-ethyl ether-n-n-tetra acetic acid 217 making and using plant cell culture media 214 total carbohydrate determination by phenol sulphate method 213 phosphate assay 210 toluene 209 water activity determination using water activity meter 208 salvage of waste mercury from brokrn thermometers, manometers and

other mercury, containing apparatus 206 refluxing apporox molar lactic acid to hydrolyse anhydrides 205 production of nisin-inactivating enzymes by two bacillus species 204 enzyme-linked immunosorbent assay (eliza) for nisin 203 production of nisin-inactivating enzymes by two bacillus species 199 dichloromethane 198 bath culture of murine hybridoma 195 production, extraction and analysis of aflatoxins 192 removal of organic solvents by rotary evaporation 191 solvent extraction of mould metabolites 188 isolation of microorganisms for soil (fungi) 184 sodium ddodecyl sulphate (SDS) 183 boric acid 180 poly acryl gel electrophoresis 177 ammonia hydraxide solution 171 trietylamine (acetate) 168 guanidium thiocyanate-used for RNA extractions 167 methanol 165 ethanol-for pracipitation of DNA and RNA 163 oligonucleotide syntesiser 162 acetonitrine (methyl cyanida) 160 tetramethylethylenediamine (temed) used for pouring polyacrylamide gel 156 phorbol myristate acetate (PMA) 154 menoclonal antibody production in vitro 153 methylene blue for staining animal cells 145 preparation,use of concentrated sodium hydroxide solution 144 preparation of 0.2% ethylendiamine tetra acetic auo salt tetrasodium salt 143 ethanol 70% 141 preparation of cell cyto-protectant 140 production and use of bouins fluid 138 potassium tellurite, used in culture media 136 preparation of phenol solutions 135 nesslers reagent, test for ammonia production 134 nalidixic acid 133 iron sulphate 7-hydrate (ferrous sulphate)

133 xanthine used in culture media 132 fast blue BB salt solution in methoxyethanol 131 heamatoxylin 129 chloroform 128 oxalic acid 127 ninhydrin reagent for rapid hippurete lest 125 cleaning immersion oil of microscope sliced and objectives using xylene 124 tween 80 used in culture media 123 thiomersal 121 preparation of stock solution of thallous acetate to use 120 preparation of nitrate broth, using sodium nitrate 119 sodium iodate 118 preparation os selenite broth, 118 effects of selenium on tumour cells 117 sodium taurocholate,sodium desoxycholate 116 preservation of biological materials using sodium azide 115 metronidazole 114 use of methanol 112 griseofulvin 111 used of glutaraldehyde to fix red cells 110 freunds adjuvant 109 formaldehyde 108 use of calcium chloride 107 butanol, propanol 106 preparation of opacity standard using barium carbonate or barium chloride105 api zyma reagent 103 amplotericin B 102 actidione (cycloheximide) in culture media 101 clocian acetic acid 100 production of stock lugols iodine 99 production of stock lactophenol 98 production of stock methylene blue 96 making up of stock aceid alcohol 95 production of stock acid ferric chloride 94 production of stock andrades indicator 93 production of stock benedicts solution 92 production od stock strong carbol fuchsin 91 production of crystal violet stain 90 production of stock borax methylene blue 89 preparation of stock kovacs reagent 88 production of stock acetone iodine 87 precipitation of nuclein acids 86 denaturing gel- to denature nucleic acid 85 E.D.T.A.(versene) buffering chelating agent 84 n,n dimethyl formamide-solvent for x-gel 83 fluorescent labelling of nucleic acids 82 dessication procedures in vacuum dryes

81 decon-90 -soaking and washidg radioactive equipmant 80 zinc chloride-for proparation of butter for phosphatase reaction 79 ammonium persulphate-catalyst for polimorisation of acrylamide gel 78 acetic acid-fixing of sequencing gel 77 nonidet NP40- penaruring of nucleic acids for PCR and nucleotide

sequencing 76 tween 20 detergent denaturant in polimesare chain reaction (PCR) 75 preparation of RNA from virus and denaturant in minipreps 74 removal of contaminants from nucleic acid suspension 73 urea-denaturing agent in sequencing gel 72 triton-x-100 71 chcloroform-removal of mineral oil 70 chelation of mercuty ions after methyl mercury hydroxide clenaturation 69 formamide 68 large scale purification of plasmid by gradient centrifugation 66 proection of cells from damage when freezing and denaturing plasmids

prior to sequencing 65 acrylamide (powder) and solution up40%, bis-acrylamide used for gel

electrophoresis 64 precipitation of nuclein acids 63 cleaning sequencing plates 62 nitrate A solution 61 making of stock nitrate B solution 60 liquid partaffin 59 production of stock 40% potassium hydroxide 58 making up of 30% trichloroacetic acid 56 zinc dust 54 making of ONPG (o-nitrophenyl-B-D-galactophyranoside) 53 production of stock sudan black 52 chloran hydrate 49 sterilisation of plastic wave using formaldehyde 48 enzyme linked immunoabsorbent assays for the antibodies 47 barbituric acid 46 sodium barditone 45 copper chloride (cupric chloride) 44 iron chloride 6-hydrate (ferric chloride) 43 benethonuim chloride crystaline 42 conversion of ADP and AMP to ATP 41 perchlaic acid 60% 40 extraction of protein from bacterial cells 36 dinitrosalicylic acid reducing sugar assay 35 protein assay 34 extraction of amino acids from bacterial cells 33 ammonium assay 31 extraction of nucleotides from bacterial cells 30 glycerol assay

29 effect of various agglutinins on normal and tumour cells, mitochondrial proliferation assay

28 isolation of cells from normal tissues and tumour tissues 27 immunocytochemistry of normal and tumour tissues 27 freezing and preservation (storage) of normal tisues, tumour tissue and

cells, denaturing of plasmids 26 monoclonal antibody production in vivo 25 culture of normal and tumour cells 24 immunocytochemistry and immunohistochemistry of normal and tumour

tissues 23 immunoblotting, western blotting, of normal and tumour homogenates 21 immunoblotting, western blotting, of normal and tumour homogenates 20 ammonia solution 35% 19 handling and diluting concentrated acids: sulphuric acid, hydrochloric

acid, nitric acid 19 handling and diluting concentrated acids: sulphuric acid, hydrochloric acid 16 darkroom procedures, developing and fixing using hydroquinone

developers 15 darkroom procedures, developing and fixing using thiosulphate fixers 14 darkroom procedures, developing and fixing films and papers with acetic

acis stop baths 13 darkroom procedures, the preparation of blue slides using diazo process 12 surface sterilisation of plant material 11 fixation, preservation and micro examination of plant material 8 plasmid elimination by acridine orange in nutrient broth 7 preparation of cloramphenicol solution 6 production of p-nitrophenol 5 production of 4-methylumbelliferone-free acid by e. coli 4 tetrox (sodium hydroxide) used in the decontamination of autoclaved

reusable glassware 3 the making up of stock solution of sodium hypochlorite 2 labsol 1 hycolin

937(a) preparation of tween-85 expanded vanadium oxide 840 (a - f) isolation and analysis of compounds from plant extracts

834(b) catalytic reaction of so2 to so3 (20 az 02), gc 834(a) catalytic reaction of so2 to so3 (20 az 02), gc, ozone generator 83, 159 ethidium bromide-used for staining nuclei acids 826(b) synthesis of calix derivative

820 (a to c) pot trial using arsenic contaminated soils (coshh assessment and atomic absorption risk assessments)

819(b) dna extraction from mycobacterium tuberculosis complex organisms (see above - this is the same assessment with a new researcher)

819(a) dna extraction from mycobacterium tuberculosis complex organisms 818(b) mycobacterium tuberculosis and tb complex organisms. sub culture (see

above - this is the same assessment with a new researcher) 818(a) mycobacterium tuberculosis and tb complex organisms. sub culture

797 (b) Colormetric assay for fungal siderophore production in liquid culture using the Chrome Azurol technique

73a urea as substrate in urease assay 71, 176 chloroform extraction 70, 166 used of mercaptoethanol in poly acrylomide gel electrophoresis 69, 170 rormamide 66, 197 dimethyl sulphoxide 65, 270 SDS polyacrylamide gel-electroforesis, preparation and loading 65, 158 acrylamide used as polymes for polyacrylamide gel 63, 212 protein/carbohydrate precipitalion by means of addition of cold acetone 60, 147 preparation of eliza substrate buffer, use of o-phenylenediamine

567ab,c,d addition to bacteria cells 565a,b,c,d making solution for addition to soil 562a,b,c,d hydrolarbons as GC standards

551d yeast nitrogen base use in microbiological media 551a,b,c chlorophenolred-B-D-galactopyranoside, Na salt, oestradiol, rubidium

chloride 550a,b,c,d engine oil to be used as carbon source in mineral media

510a,b handling of human skin tissue 51, 215 chemical sterilization of equipment and apparatus 51, 169 formaldehyde solution 51, 150 decontamination of sefty cabinets 51, 150 decontamination of safety coabinets

51, 142, 187 preparation of cell fixative solution 482c handling of acanthamoeba castellanii (douglas) 482b observation and cultivation of microorganisms from plants,soil amd water 482a handling hazard group 2 micro-organisms 460b extraction beffer preparation, use sodium acetate 460a extraction buffer solution 459a cell culture assesment-elisa

403, 403a pholloidin (tric-labelled)-fluoresence staining of acting filaments 395a,b baf fmt 30-used as an antifroam in fermentation 377a belousou-zhabotinsky reaction

37, 38, 39 schinder orcinol assay for rna 365a,b ethyleneglucol

336, 355 antibiotic assay 2a teepol

296, 297 reducing sugar estimation (nelsone somogii) 292, 310 assay for peroxide/hydrogen peroxide 288, 330 trypan blue for the determination of cell densities 288, 329 2nd year virology practical classes 288, 322 animal cell practical trypan blue 274, 278 piperidine 274, 277 hydroxybenzotriazole 274, 276 ethanediol 274, 275 acetic anhydride 220, 270 isoamyl alcohol-use in preparation of extraction mixes

210, 273 preparation of formazin turbidium standards used to calibrate turbidium meter

210, 273 preparation of formazin stock solution for turbidily meter calibration 183a boric acid

183, 229 boric acid 168, 405 puritication of mini prop DNA by use of guanidinium thocyanate method 162, 189 high performance liquid chromatography (HPLC) procedure

157b preparation of MTT solution for colorimetric assay of cell growth and viability

156a, 172 use of phorbol myristate acetate (PMA) to induce COX-2 expression in prostate cosncer cell lines

145, 323 sodium hydroxide solution 145, 186 sodium hydroxide (NAOH) 137/147 o-phenylenediamine dihydracholide 137, 147 preparation of o-phenylenediamine substrate buffer for eliza assay 136, 173 phenol extraction

126a,b,c,d,e mineral media 124, 232 twee 80 used in culture media

123, 208, 249 use of thiomersal in eliza sobstrate buffer 107, 223 isopropanol-precipitation of nuclein acids 107, 179 isopropanol-precipitation of DNA and preparation of isopropanol saturated

with caesuim chloride 102, 248 cycloheximide (actidione) 101, 161 acetic acid

434,436,437 phospotase assay (soil) 309,350,358 nisin elisa-peroxidase conjugate 193,195,284 derivatisation of aflatoxin

168,173,181 RNAzol-b- extraction of RNA 71,114,187 thin layer chromatography procedures (TLC)

373,374 spiking of metalworking fluid emulsion with compand location 369,370 sbs 1-9 practical cell and molecular biology 352,345 n-hexadecane in culture medzia 351,359 handling of pyridine 348,357 conjugate syntesis-sodium periodate 347,354 antibiotic assay 346,353 use of thrombin to clean proteins 266,340 production of feeder cells for hybrid cell production 114,279 preparation of 0.1% crystal violet, 10% methanol solution for cell staining