HPLC and HPTLC Standardazation [Read-Only]

21
n o i t a z i d r a d n a t s C L T P H d n a C L P H g u r d e d u r c f o r a k s a l a K . G n a h o M . r D , h c r a e s e R d n a n o i t a c u d E l a c i t u e c a m r a h P f o e t u t i t s n I l e t a P . C . R a i d n I , S M , r u p r i h S

description

Standardazation of crude drug

Transcript of HPLC and HPTLC Standardazation [Read-Only]

  • noitazidradnats CLTPH dna CLPH gurd edurc fo

    raksalaK .G nahoM .rD ,hcraeseR dna noitacudE lacituecamrahP fo etutitsnI letaP .C .R

    aidnI ,SM ,ruprihS

  • CLPH:ot derrefer yllanigiro

    H hgi P erusser L diuqi C yhpargotamorh

    reporp wolla ot ezis elcitrap llams esu ot elba eb ot erusserp hgihsetar wolf elbanosaer ta noitarapes

    ylretaL :ot derrefer

    H hgi P ecnamrofre L diuqi C yhpargotamorh

    ytilibicudorper sti ot eud ecnamrofrep hgih

    otno erutxim elpmas a gnitcejni yb detarapes era sdnuopmoC .nmuloc eht

    nmuloc eht hguorht ssap erutxim eht ni tnenopmoc tnereffid ehT noititrap rieht ni secnereffid ot eud setar tnereffid ta roivaheb .esahp yranoitats eht dna esahp elibom eht neewteb

    detarapes suhT

    :ot derrefer yllanigiro

    H hgi P erusser L diuqi C yhpargotamorh

    reporp wolla ot ezis elcitrap llams esu ot elba eb ot erusserp hgihsetar wolf elbanosaer ta noitarapes

    ylretaL :ot derrefer

    H hgi P ecnamrofre L diuqi C yhpargotamorh

    ytilibicudorper sti ot eud ecnamrofrep hgih

    otno erutxim elpmas a gnitcejni yb detarapes era sdnuopmoC .nmuloc eht

    nmuloc eht hguorht ssap erutxim eht ni tnenopmoc tnereffid ehT noititrap rieht ni secnereffid ot eud setar tnereffid ta roivaheb .esahp yranoitats eht dna esahp elibom eht neewteb

    detarapes suhT

  • elpicnirP esahp tS diuqil esahp eliboM diuqil

    tneiciffeoc noititrap a ni dnuopmoc a fo snoitartnecnoc fo oitar eht si

    sesahp elbicsimmi owt fo erutxim

  • diuqil( noititraP - )diuqil

    esreveR - esahp

    noN - esahp yranoitats ralop

    lamroN - esahp

    esahp yranoitats raloP

    metsyS hpargotamorhC diuqiL A fO stnenopmoC riovreseR tnevloS / esahP eliboM ressageD )pmuP( metsyS yrevileD tnevloS rotcejnI nmulocerP nmuloC lortnoC erutarepmeT srotceteD )noitcelloC ataD( redroceR

    diuqil( noititraP - )diuqil

    esreveR - esahp

    noN - esahp yranoitats ralop

    lamroN - esahp

    esahp yranoitats raloP

    metsyS hpargotamorhC diuqiL A fO stnenopmoC riovreseR tnevloS / esahP eliboM ressageD )pmuP( metsyS yrevileD tnevloS rotcejnI nmulocerP nmuloC lortnoC erutarepmeT srotceteD )noitcelloC ataD( redroceR

  • CLPH metsys

    riovreseR tnevloS

    ressageD

    metsyS yrevileD tnevloS)pmuP(

    rotcejnI

    nevo& nmuloC

    srotceteD )noitcelloC ataD( redroceR

    7

    riovreseR tnevloS

    ressageD

    metsyS yrevileD tnevloS)pmuP(

    rotcejnI

    nevo& nmuloC

    srotceteD )noitcelloC ataD( redroceR

  • CLPH fo segatnavdA sisylana fo deeps dna noituloser rehgiH

    ro gnikcaper tuohtiw desuer eb nac snmuloc CLPHnoitareneger

    eht fo lortnoc esolc ot eud ytilibicudorper retaerGnoitarapes fo ycneiciffe eht gnitceffa sretemarap

    atad dna noitarepo tnemurtsni fo noitamotua ysaEsisylana

    egral ot ytilibatpadA - ,elacs

    serudecorp evitaraperp

    sisylana fo deeps dna noituloser rehgiH

    ro gnikcaper tuohtiw desuer eb nac snmuloc CLPHnoitareneger

    eht fo lortnoc esolc ot eud ytilibicudorper retaerGnoitarapes fo ycneiciffe eht gnitceffa sretemarap

    atad dna noitarepo tnemurtsni fo noitamotua ysaEsisylana

    egral ot ytilibatpadA - ,elacs

    serudecorp evitaraperp

  • hgemlaK

  • noitanimreted evitatilauQ

    TR - nim 3vU mn 452

  • noitanimreted evitatitnauQ noitaraperp dradnatS

    fo snoitulid desaercni laudarg eht eraperP edilahpargordna nwonk fo cnoc 02( - 001 lonahtem ni )lm/g

    noitaraperp elpmaS nwonK cnoc )lm/g 001( tcartxe fo

    erudecorP 02 tcejnI elpmas yb dewollof sdradnats fo .cnoc tnereffid fo l sV CUA( dradnats fo hparg noitarbilac tolp cnoC )

  • dradnatS

    TR - nim 3vU mn 452

    tcartxe tseT

    TR - nim 3vU mn 452

  • noitauqe noissergeR C + XM=YY( =X - M/)C

    erehW nwonknu fo CUA =Y nwonknu fo .cnoc =X

    noitauqe noissergeR C + XM=YY( =X - M/)C

    erehW nwonknu fo CUA =Y nwonknu fo .cnoc =X

  • CTLPH CLTPH -

    .yhpargotamorhC reyaL nihT ecnamrofreP hgiH

    .CLT fo mrof detamotua & detacitsihpoS

    elpicnirP ot eud tluser yam noitarapeS

    htob yb ro noititrap ro noitprosda eht nopu gnidneped nonemonehp

    setalp no desu stnebrosda fo erutan rof desu metsys stnevlos dna

    .tnempoleved noitprosdA - evitaler no desab

    esahp yranoitats drawot ytiniffa

    ytiniffa sseL

    CLTPH -.yhpargotamorhC reyaL nihT ecnamrofreP hgiH

    .CLT fo mrof detamotua & detacitsihpoS

    elpicnirP ot eud tluser yam noitarapeS

    htob yb ro noititrap ro noitprosda eht nopu gnidneped nonemonehp

    setalp no desu stnebrosda fo erutan rof desu metsys stnevlos dna

    .tnempoleved noitprosdA - evitaler no desab

    esahp yranoitats drawot ytiniffa ytiniffa eroM

    ytiniffa etaredoM

  • CLT & CLTPH neewteb ecnereffiD

    CLTPH CLT ezis elcitraP 4-8 m 5- 02 m reyal tnebroS

    ssenkciht 001 m 052 m sseL hgiH ycneiciffE snoitarapeS 3-5 mc 01 - mc51 rewolS retsaF emit sisylanA tnempoleveD

    rebmahc relpmas otuA gnittops elpmaS gnittops launaM retemotisneD .gninnacS gnol ro trohs vU

    CLTPH CLT ezis elcitraP 4-8 m 5- 02 m reyal tnebroS

    ssenkciht 001 m 052 m sseL hgiH ycneiciffE snoitarapeS 3-5 mc 01 - mc51 rewolS retsaF emit sisylanA tnempoleveD

    rebmahc relpmas otuA gnittops elpmaS gnittops launaM retemotisneD .gninnacS gnol ro trohs vU

    5102/31/3 51

    fo tnuoma sseLesahp elibom

    tnuoma eroMesahp .bom fo

  • NEEWTEB ECNEREFFIDCLPH & CLTPH

    CLTPH CLPHsuoenatlumiS gnissecorp suoenatlumis ssecorp

    fo elpmas & dradnats fo elpmas & dts . tonrednu emas noitidnoc elbissop

    emertxE ytilibixelf rof detimiL ytilibixelfsuoirav spets

    ,yllacinhceT ti si elpmis dellikS & llew - deniartot nrael & etarepo lennosrep era dedeen

    elpmas noitaraperp si elpmis elpmas noitaraperp si lacitirc

    elpmas fo tnrereffid emulov nac elpmas fo emas lov ylno deilppanac deilppa

    CLTPH CLPHsuoenatlumiS gnissecorp suoenatlumis ssecorp

    fo elpmas & dradnats fo elpmas & dts . tonrednu emas noitidnoc elbissop

    emertxE ytilibixelf rof detimiL ytilibixelfsuoirav spets

    ,yllacinhceT ti si elpmis dellikS & llew - deniartot nrael & etarepo lennosrep era dedeen

    elpmas noitaraperp si elpmis elpmas noitaraperp si lacitirc

    elpmas fo tnrereffid emulov nac elpmas fo emas lov ylno deilppanac deilppa

    61

  • oitavitcA setalp fo n

  • hgemlaK

  • dradnatS