How to improve simple experiments - · PDF fileWater potential of potato •Use mannitol or...
Transcript of How to improve simple experiments - · PDF fileWater potential of potato •Use mannitol or...
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How to improve simple experiments
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Rate of photosynthesis
Quantitative IV and DV Qualitative IV and DV
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Vitamin C content of fruit /vegetables
Connect with problems:
• Compare different cooking methods: streaming, boiling, microwaving
• Verify the vitamin C content of vitamin C tablet
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Vitamin C titration with potassium iodate
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Water potential of potato
• Use mannitol or salt solution to replace sucrose solution
• Replicate with different potato tubers
• Use Chardakov method to check the result
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Sucrose solution with potato
Add a drop of methylene blue after 0.5 hour
Release a drop of the blue solution in
same conc. of sucrose solution
Chardakov method
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Transpiration rate • DVs: water absorption & weight
• IVs: light intensity, wind speed, leaf surface area, upper/lower epidermis
• Quantitative measures of IV: light intensity by light meter, wind speed by anemometer
• Within or between subject design
• Replications with more shoots
• Use dye in place of water to identify xylem in stem
• Count the density of stomata in upper/lower epidermis
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Amylase activity
• IV: pH, T, amylase conc.
• Measure amylase activity:
Time for complete hydrolysis of starch as detected by repeated iodine test
Amount of starch hydrolyzed in a fixed period of time
Amount of maltose produced as detected by DNS
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Amount of maltose detected by DNS
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Absorbance =Log10(I0/I)
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Thank you!
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Turning biotech experiments into SBA investigations
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Digestion and ligation of plasmids
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Check transformation of bacteria
control