Hormones in the Environment - uni-stuttgart.de · A B C 1 2 denitrification tank aeration tank...
1
A B C 1 2 denitrification tank aeration tank rotating biological disk contactors secondary clarifiers (1 – 3) 3 oxidation ditch secondary clarifier (oxidation ditch) micro sieves effluent Biological Wastewater Treatment grids and sieves A rotating biological disk contactors oxidation ditch influent aerated grit chamber primary clarifier B C aerated sludge process Mechanical Wastewater Treatment Results (mechanical wastewater treatment): EEQ values of the influent and the effluent of the primary clarifier taking into account the average hydraulic retention time (Nov 8, 2006) Hormones in the Environment - Quantitation of the Total Estrogenic Activity of Wastewater Samples with a Bioassay www.iswa.uni-stuttgart.de Contact: Prof. Dr. rer. nat. Jörg W. Metzger, ISWA, Bandtäle 2, 70569 Stuttgart [email protected], www.iswa.uni-stuttgart.de/ch Cornelia Klaus, Biljana Marić, Andrea Kern, Bertram Kuch and Jörg W. Metzger Institute for Sanitary Engineering, Waste Water Quality and Solid Waste Management, Dept. of Hydrochemistry Universität Stuttgart Aim of this Study: • Input/output balance of the total estrogenic activity in the sewage treatment plant (STP) of Stuttgart-Büsnau in Germany • Determination of the efficiencies of purification of the three different biological treatment steps Material and Methods: Sampling: grab samples of each sewage treatment step taking into account the hydraulic retention time if possible in addition: 24 hours composite samples of influent and effluent during one week Sample Treatment: centrifugation filtration solid phase extraction (RP-18) Determination of Estrogenicity: biological summary parameter expressed as an estradiol equivalent concentration (EEQ) in ng/L E-Screen assay: in vitro cell proliferation assay based on the human estrogen receptor-positive breast cancer cell-line MCF-7 general estimation of the uncertainty of measurement as part of the validation process for the suitability of this bioassay • • • • • • • • Results (24 h composite samples): Diminution of estrogenic activity during wastewater treatment - EEQ values of 24 h composite samples (Nov 21 - 28, 2006) Conclusions: • The E-Screen assay is very suitable for balancing estrogenic activity of wastewater samples (data variation max. one order of magnitude) • EEQ values of the influent ranged between 12.6 ng/L and 72.7 ng/L (median 23.8 ng/L); those of the effluent were found to be permanently below 2.5 ng/L (median 1.6 ng/L) • The overall elimination efficiency of the whole plant is constant and stable: mechanical wastewater treatment did not lead to any significant elimination, whereas biological treatment reduced estrogenicity to a great extent with slight differences between the three technologies • There is a small but constant input of estrogen active compounds into the receiving water: information on the dilution factor and the kind of different estrogenic compounds is required to evaluate an ecotoxicological risk potential Results (biological wastewater treatment): Elimination of estrogenic activity in wastewater Elimination of estrogenic activity in wastewater - activated sludge process - rotating biological disk contactor Elimination of estrogenic activity in wastewater - oxidation ditch 0,0 10,0 20,0 30,0 40,0 50,0 60,0 70,0 80,0 EEQ (ng/L) influent primary clarifier denitrification tank aeration tank secondary clarifier 1 secondary clarifier 2 secondary clarifier 3 effluent Oct 11, 2006 Oct 18, 2006 Jan 24, 2007 0,0 10,0 20,0 30,0 40,0 50,0 60,0 70,0 80,0 influent primary clarifier roll 1 roll 2 roll 3 secondary clarifier 1 effluent EEQ (ng/l) Oct 18, 2006 Jan 10, 2007 Jun 4, 2007 0,0 5,0 10,0 15,0 20,0 25,0 30,0 35,0 40,0 EEQ (ng/L) influent oxidation ditch secondary clarifier (OD) effluent Oct 12/13, 2006 Oct 17/18, 2006 Jan 16/17, 2007 Reference: Klaus, Cornelia (2007): Ermittlung des Vorkommens und der Elimination von estrogen aktiven Substanzen im Lehr- und Forschungsklärwerk Stuttgart-Büsnau - Bewertung des E-Screen Assays als in vitro- Testverfahren, diploma thesis, Universität Stuttgart → no significant decrease in estrogenicity → average efficiency of removal during that week: 92.2 % Elimination efficiencies (%) of biological wastewater treatment (effluent of the biological tank / raw influent) 1. sampling 2. sampling 3. sampling average activated sludge process 92.3 97.8 94.4 94.8 rotating biological disk contactors 66.7 82.3 76.3 75.5 oxidation ditch 91.0 90.5 93.7 91.7 0,0 5,0 10,0 15,0 20,0 25,0 30,0 35,0 40,0 45,0 50,0 EEQ (ng/L) Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday influent effluent 0 5 10 15 20 25 30 EEQ (ng/L) 7.30 a.m. / 8.55 a.m. 7.45 a.m. / 9.10 a.m. 8.00 a.m. / 9.25 a.m. 8.15 a.m. / 9.40 a.m. influent effluent primary clarifier sampling sites (grab samples)
Transcript of Hormones in the Environment - uni-stuttgart.de · A B C 1 2 denitrification tank aeration tank...
A
B
C
1
2
denitrification tank aeration tank
rotating biologicaldiskcontactors
secondaryclarifiers(1– 3)
3
oxidationditch
secondaryclarifier(oxidationditch)
microsieves
effluent
grids andsieves
aerated sludge process (A)rotating biological disk contactors (B)oxidation ditch (C)
influent
aeratedgritchamber primaryclarifier
Biological Wastewater Treatment
A
B
C
1
2
denitrification tank aeration tank
rotating biologicaldiskcontactors
secondaryclarifiers(1– 3)
3
oxidationditch
secondaryclarifier(oxidationditch)
microsieves
effluent
grids andsieves
Arotating biological disk contactors
oxidation ditch
influent
aeratedgritchamber primaryclarifier
BC
aerated sludge process
Mechanical Wastewater Treatment
Results (mechanical wastewater treatment): EEQvaluesoftheinfluentandtheeffluentoftheprimaryclarifiertaking intoaccounttheaveragehydraulicretentiontime(Nov8,2006)
Hormones in the Environment - Quantitation of the Total Estrogenic Activity
of Wastewater Samples with a Bioassay
www.iswa.uni-stuttgart.de
Contact
: Prof.Dr.rer.nat.JörgW.Metzger,ISWA,Bandtäle2,70569Stuttgart
[email protected],www.iswa.uni-stuttgart.de/ch
CorneliaKlaus,BiljanaMarić,AndreaKern,BertramKuchandJörgW.Metzger InstituteforSanitaryEngineering,WasteWaterQualityandSolidWasteManagement,Dept.ofHydrochemistry
Universität Stuttgart
Aim of this Study: • Input/output balance of the total estrogenic activity in the sewage treatment plant (STP) of Stuttgart-Büsnau in Germany • Determination of the efficiencies of purification of the three different biological treatment steps
Material and Methods:Sampling: grabsamplesofeachsewage treatmentsteptakingintoaccount thehydraulicretentiontimeifpossibleinaddition:24hourscomposite samplesofinfluentandeffluent duringoneweekSampleTreatment: centrifugation filtration solidphaseextraction(RP-18)DeterminationofEstrogenicity: biologicalsummaryparameter expressedasanestradiolequivalent concentration(EEQ)inng/LE-Screenassay:invitrocell proliferationassaybasedonthe humanestrogenreceptor-positive breastcancercell-lineMCF-7 generalestimationoftheuncertainty ofmeasurementaspartofthe validationprocessforthesuitabilityof thisbioassay
•
•
•••
•
•
•
Results (24 h composite samples): Diminutionofestrogenicactivityduringwastewatertreatment -EEQvaluesof24hcompositesamples(Nov21-28,2006)
Conclusions:
• TheE-Screenassayisverysuitablefor balancingestrogenicactivityofwastewater samples(datavariationmax.oneorderof magnitude)• EEQ values of the influent rangedbetween 12.6ng/Land72.7ng/L(median23.8ng/L); those of the effluentwerefoundtobe permanentlybelow2.5ng/L(median1.6ng/L)
• Theoveralleliminationefficiencyofthewholeplantisconstantand stable:mechanicalwastewatertreatmentdidnotleadtoany significantelimination,whereasbiologicaltreatmentreducedestrogenicity toagreatextentwithslightdifferencesbetweenthethreetechnologies• Thereisasmallbutconstantinputofestrogenactivecompounds intothereceivingwater:informationonthedilutionfactorandthekindof differentestrogeniccompoundsisrequiredtoevaluateanecotoxicological riskpotential
Results (biological wastewater treatment): Eliminationofestrogenicactivityinwastewater Eliminationofestrogenicactivityinwastewater -activated sludge process -rotating biological disk contactor
Eliminationofestrogenicactivityinwastewater -oxidation ditch
0,0
10,0
20,0
30,0
40,0
50,0
60,0
70,0
80,0
EEQ(ng/L)
influent primaryclarif ier
denitrif icationtank
aerationtank secondaryclarif ier1
secondaryclarif ier2
secondaryclarif ier3
eff luent
Oct11,2006 Oct18,2006 Jan24,2007
0,0
10,0
20,0
30,0
40,0
50,0
60,0
70,0
80,0
influent primaryclarif ier
roll1 roll2 roll3 secondaryclarif ier1
effluent
EEQ(ng/l)
Oct18,2006 Jan10,2007 Jun4,2007
0,0
5,0
10,0
15,0
20,0
25,0
30,0
35,0
40,0
EEQ(ng/L)
influent oxidationditch secondaryclarif ier(OD)
effluent
Oct12/13,2006 Oct17/18,2006 Jan16/17,2007
Reference: Klaus,Cornelia(2007):ErmittlungdesVorkommensundderElimination vonestrogenaktivenSubstanzenimLehr-undForschungsklärwerkStuttgart-Büsnau -BewertungdesE-ScreenAssaysalsinvitro-Testverfahren,diplomathesis,UniversitätStuttgart
→ nosignificant decreasein estrogenicity
→ average efficiencyof removalduring thatweek: 92.2%
Eliminationefficiencies(%)ofbiologicalwastewatertreatment (effluentofthebiologicaltank/rawinfluent)
1.sampling 2.sampling 3.sampling average
activatedsludgeprocess 92.3 97.8 94.4 94.8
rotatingbiologicaldiskcontactors 66.7 82.3 76.3 75.5
oxidationditch 91.0 90.5 93.7 91.7
0,0
5,0
10,0
15,0
20,0
25,0
30,0
35,0
40,0
45,0
50,0
EEQ(ng/L)
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
influent effluent
0
5
10
15
20
25
30
EEQ(ng/L)
7.30a.m./8.55a.m. 7.45a.m./9.10a.m. 8.00a.m./9.25a.m. 8.15a.m./9.40a.m.
inf luent eff luentprimaryclarif ier
samplingsites(grabsamples)
