Horiba ABX Micros 60 - Technical Manual

7/16/2019 Horiba ABX Micros 60 - Technical Manual

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3. MAINTENANCE PROCEDURES

2. ELECTRIC & ELECTRONIC PRINCIPLES

1. HYDRAULIC & PNEUMATIC PRINCIPLES

4. OUTPUT FORMAT

CONTENTS

INDEX P/N REVISION REVISION SECTION DATE

A Creation all 30/03/98

B RAH 581 AA Miscellaneous corrections 1, 3, 4 07/10/98

REVISIONS

5. TRAINING SLIDES



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CONTENTS

1. GENERALITIES ..................................................................................................................................... 2

2. MICROS 60 OT HYDRAULIC................................................................................................................. 42.1. With bottles ..................................................................................................................................... 4

2.1.1. Transmission tubes list ........................................................................................................... 42.1.2. Hydropneumatic connections ................................................................................................. 5

2.2. Pack ............................................................................................................................................... 62.2.1. Transmission tubes list ........................................................................................................... 62.2.2. Hydropneumatic connections ................................................................................................. 7

2.3. Hydraulic cycle description ............................................................................................................. 82.3.1. Atmosphere circuit .................................................................................................................. 82.3.2. Diluent circuit .......................................................................................................................... 82.3.3. Clean circuit ............................................................................................................................ 82.3.4. Lyse circuit .............................................................................................................................. 82.3.5. WBC/RBC counting circuit ...................................................................................................... 82.3.6. Drain/bubbling circuit .............................................................................................................. 8

3. MICROS 60 CT HYDRAULIC ............................................................................................................... 15

3.1. With bottles ................................................................................................................................... 153.1.1. Transmission tubes list ......................................................................................................... 153.1.2. Hydropneumatic connections ............................................................................................... 16

3.2. Pack ............................................................................................................................................. 173.2.1. Transmission tubes list ......................................................................................................... 173.2.2. Hydropneumatic connections ............................................................................................... 18

3.3. Hydraulic cycle description ........................................................................................................... 193.3.1. Atmosphere circuit ................................................................................................................ 193.3.2. Diluent circuit ........................................................................................................................ 193.3.3. Clean circuit .......................................................................................................................... 193.3.4. Lyse circuit ............................................................................................................................ 193.3.5. WBC/RBC counting circuit .................................................................................................... 19

3.3.6. Drain/bubbling circuit ............................................................................................................ 19

4. PNEUMATIC DIAGRAMS..................................................................................................................... 26

4.1. Micros 60 CT bottle version ............................................................................................... ........... 264.2. Micros 60 CT pack version ........................................................................................................... 264.3. Micros 60 OT bottle version ............................................................................................... ........... 264.4. Micros 60 OT pack version ........................................................................................................... 26



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1. GENERALITIES

MICROS 60 instrument has been designed for simple mechanical operations.4 stepper motors provide movements to mechanical assemblies.Pressure and vacuum are provided by the vacuum/waste syringe up and down movements(diag 1).Liquid movements are achieved either by means of mechanical assembly movements (liquid

syringes diag 2) or pressure/vacuum and simultaneous action of specific valves.

• Dilution chambers (Diag .3)

WBC/HGB and RBC chambers are made of GRILAMID TR55 LY injected.The diode and the cell of the spectrophotometer are glued on the WBC/HGB chamber.Chamber positions can be modified in order to obtain the best sampling position possible.

Dilutions : First dilution is carried out in the WBC/HGB chamber (with a bubbling phasis).The RBC blood sample is aspirated from this dilution.Lyse is sent from the drain nipple of the WBC/HGB chamber.Counts have a duration of 2 x 6 seconds.

(see procedures RAS 188 A and RAS 187 A for cycle hydraulic details)

Diag.1 Diag.2

Rinse : To obtain the best rinse in the counting heads, diluent is sentfrom the liquid syringes. This is carried out before, betweenand after the two counts.



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IMPORTANT A window on the HGB/WBC chamber allows the needle to move down into the chamberand to inject reagents. As important light or variation of light can cause HGB result

drifts, close instrument cover and door before running blood analyses.

Bubbling :Insulators avoid polluted liquid overflows during bubbling phasis. they also allows an accurateadjustment of the bubbling volume.

MICROS 60 CT specifics : - The piercing needle is equipped with two injectors to obtain a homogeneous diluent flowduring needle rinsing phasis (see procedures RAS 188 A and RAS 187 A).- Atmosphere is provided to sample tubes to allow a correct aspiration of blood.



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DESIGNATION PART NUMBER DIAMETER LENGTH QUANTITY

SLEEVE HPS3 DBD005A 5-9 0.5

T CONNECTOR EAB006B 2.3 3

T CONNECTOR EAB032A 1.5 1

TUBE CAP EAC017A 2.5 1

TYGON TUBE 0.051" EAE006A 1.30 140 1

TYGON TUBE 0.051" EAE006A 1.30 350 1

TYGON TUBE 0.060" EAE007A 1.52 20 1

TYGON TUBE 0.060" EAE007A 1.52 40 3

TYGON TUBE 0.060" EAE007A 1.52 50 1

TYGON TUBE 0.060" EAE007A 1.52 60 1

TYGON TUBE 0.060" EAE007A 1.52 70 2

TYGON TUBE 0.060" EAE007A 1.52 80 1

TYGON TUBE 0.060" EAE007A 1.52 150 1

TYGON TUBE 0.060" EAE007A 1.52 170 1

TYGON TUBE 0.060" EAE007A 1.52 220 1

TYGON TUBE 0.060" EAE007A 1.52 240 1

TYGON TUBE 0.060" EAE007A 1.52 300 1

TYGON TUBE 0.060" EAE007A 1.52 370 1TYGON TUBE 0.060" EAE007A 1.52 410 1

TYGON TUBE 0.060" EAE007A 1.52 450 2

TYGON TUBE 0.060" EAE007A 1.52 480 1

TYGON TUBE 0.081" EAE008A 2.05 20 1

TYGON TUBE 0.081" EAE008A 2.05 35 1

TYGON TUBE 0.081" EAE008A 2.05 200 1

TYGON TUBE 0.081" EAE008A 2.05 330 1

TYGON TUBE 0.081" EAE008A 2.05 1080 1

TYGON TUBE 0.090" EAE009A 2.28 20 2

TYGON TUBE 0.090" EAE009A 2.28 50 1

TYGON TUBE 0.090" EAE009A 2.28 60 2

TYGON TUBE 0.090" EAE009A 2.28 120 1

TYGON TUBE 0.090" EAE009A 2.28 140 1

TYGON TUBE 0.090" EAE009A 2.28 150 1

TYGON TUBE 0.090" EAE009A 2.28 190 1

BLUE TYGON TUBE 0.090" EAE036A 2.28 1100 1

SLEEVE GAL098A 30

TUBE SHIELD GBC088A 4.4 30 1


2.1. With bottles

2.1.1. Transmission tubes list

Liquid circuits, hydropneumatic connections, as well as the transmission tubes used, aredescribed in the following chart tables.

2. MICROS 60 OT HYDRAULIC



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CIRCUIT FROM SLEEVE DIAM LENGTH TO SLEEVE

AIR(atmosphere) 2.28 190 Liquid valve 2_2

Liquid valve 2_1 Y 2.28 50 Waste-chamb._1 Y

Waste-chamb._2 2.28 20 Cap EAC017A

DILUENT

Diluent input 2.05 330 Liquid valve11_1

Liquid valve11_3 Y 1.52 220 Temp. sensor xba281a Y

Temp. sensor. xba281a Y 1.52 40 Liquid syringes_3 Y

Liquid valve11_2 Y 1.52 40 Liquid valve10_3 Y

Liquid valve10_1 Y 1.52 70 Liquid valve7_3 Y

Liquid valve7_1 Y 1.52 370 needle rinsing block._2

Needle rinsing block._1 1.52 410 Liquid valve8_1

Liquid valve8_2 Y 1.52 300 Waste-chamb._3

Liquid valve 7_2 Y 1.52 240 Té 2.3_1

Té 2.3_2 1.52 50 Bac WBC/HGB_3

Liquid valve10_2 Y 1.30 140 Liquid syringes_1 Y

Liquid syringes_2 Y 1.30 350 needle_1

Liquid syringes_2 Y Needle_1 Y

CLEANClean bottle 2.28 1100 blue Liquid valve4_2 Y

Liquid valve4_1 Y 1.52 450 T connector 2.3_3

LYSE

Lyse bottle 2.05 1080 liquid valve 1_1

Liquid valve1_3 1.52 150 Liquid syringes _4

Liquid valve1_2 1.52 480 WBC grounding connector

WBC grounding connector 1.52 20 T connector 1.5_1

WBC/RBC

COUNTING

WBC/HGB_2 chamber 1.52 170 RBC_3 chamber

RBC_2 chamber 1.52 450 liquid valve 6_2 Y

Liquid valve 6_1 Y 1.52 60 Waste-chamb._4

DRAIN/BUBBLING

WBC/HGB_1 chamber 1.52 40 T connector 1.5_2

WBC/HGB_1 chamber gbc088a 30 T connector 1.5_2

T connector 1.5_3 1.52 80 Insulator WBC_1

Insulator WBC_2 2.28 120 liquid valve12_2 Y


RBC_1 chamber 1.52 70 Insulator RBC_1

RBC_1 chamber gbc088a 60 Insulator RBC_1

Insulator RBC_2 2.28 150 liquid valve13_2 Y


T connector 2.3_3 2.05 200 T connector 2.3_2

T connector 2.3_3 2.28 20 Cell xba199a Y

Cell xba199a Y 2.28 60 Waste-chamb._5 Y

T connector 2.3_1 2.28 60 Liquid valve5_2 Y

Liquid valve5_1 2.28 140 Waste ouput

NOTE Read this table as follows in this example :The Liquid valve 7_2 corresponds to the ouput 2 of the valve number 7 (seeattached pneumatic diagram).

2.1.2. Hydropneumatic connections



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2.2. Pack



SLEEVE HPS3 DBD005A 5-9 0.5




TYGON TUBE 0.051" EAE006A 1.30 140 1

TYGON TUBE 0.051" EAE006A 1.30 350 1

TYGON TUBE 0.060" EAE007A 1.52 20 1

TYGON TUBE 0.060" EAE007A 1.52 40 3

TYGON TUBE 0.060" EAE007A 1.52 50 1

TYGON TUBE 0.060" EAE007A 1.52 60 1

TYGON TUBE 0.060" EAE007A 1.52 70 2

TYGON TUBE 0.060" EAE007A 1.52 80 1

TYGON TUBE 0.060" EAE007A 1.52 150 1

TYGON TUBE 0.060" EAE007A 1.52 170 1

TYGON TUBE 0.060" EAE007A 1.52 220 1

TYGON TUBE 0.060" EAE007A 1.52 240 1

TYGON TUBE 0.060" EAE007A 1.52 300 1

TYGON TUBE 0.060" EAE007A 1.52 370 1

TYGON TUBE 0.060" EAE007A 1.52 410 1

TYGON TUBE 0.060" EAE007A 1.52 450 2


TYGON TUBE 0.081" EAE008A 2.05 35 1

TYGON TUBE 0.081" EAE008A 2.05 200 1

TYGON TUBE 0.081" EAE008A 2.05 590 1

TYGON TUBE 0.090" EAE009A 2.28 20 2

TYGON TUBE 0.090" EAE009A 2.28 50 1

TYGON TUBE 0.090" EAE009A 2.28 60 2

TYGON TUBE 0.090" EAE009A 2.28 120 1

TYGON TUBE 0.090" EAE009A 2.28 150 1

TYGON TUBE 0.090" EAE009A 2.28 190 1

TYGON TUBE 0.090" EAE009A 2.28 510 1

TYGON TUBE 0.090" EAE009A 2.28 550 1

TYGON TUBE 0.090" EAE009A 2.28 1100 1

SLEEVE GAL098A 31



METALLIC SHEATH GBC170A 5.2 35 3



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CIRCUIT FROM SLEEVE DIAMETER LENGTH TO SLEEVE

AIR

(atmosphere) 2.28 190 Liquid valve 2_2


Waste-chamb._2 2.28 20 cap EAC017A

DILUENT

Pack_3 (Diluent) 2.28 550 Liquid valve 11_1 Y

Pack_3 (Diluent) gbc170a

Liquid valve 11_3 Y 1.52 220 temp. sensor xba281a Y

temp. sensor xba281a Y 1.52 40 Liquid syringes _3 Y

Liquid valve 11_2 Y 1.52 40 Liquid valve 10_3 Y


Liquid valve 7_1 Y 1.52 370 Needle rinsing block_2

needle rinsing block._1 1.52 410 Liquid valve 8_1


Liquid valve 7_2 Y 1.52 240 T connector 2.3_1

T connector 2.3_2 1.52 50 Bac WBC/HGB_3

Liquid valve 10_2 Y 1.30 140 Liquid syringes _1 Y

Liquid syringes _2 Y 1.30 350 NEEDLE_1

Liquid syringes _2 Y NEEDLE_1 Y

CLEAN

Pack-1 (Clean) 2.28 510 Liquid valve 4_2 Y

Pack-1 (Clean) gbc170a


LYSE

Pack_2 (Lyse) 2.05 590 Liquid valve 1_1

Pack_2 (Lyse) gbc170a

Liquid valve 1_3 1.52 150 Liquid syringes _4

Liquid valve 1_2 1.52 480 WBC grounding connector


WBC/RBC

counting

WBC/HGB chamber _2 1.52 170 RBC chamber _3

RBC chamber _2 1.52 450 Liquid valve 6_2 Y


DRAIN /

BUBBLING

WBC/HGB chamber _1 1.52 40 T connector 1.5_2

WBC/HGB chamber _1 gbc088a 30 T connector 1.5_2

T connector 1.5_3 1.52 80 Insulator WBC_1

Insulator WBC_2 2.28 120 Liquid valve 12_2 Y


Bac RBC_1 1.52 70 Insulator RBC_1

Bac RBC_1 gbc088a 60 Insulator RBC_1

Insulator RBC_2 2.28 150 Liquid valve 13_2 Y



T connector 2.3_3 2.28 20 Cellule xba199a Y


T connector 2.3_1 2.28 60 Liquid valve 5_2 Y

Liquid valve 5_1 2.28 1100 Pack_4 (Waste)

NOTE

Read this table as follows in this example :The Liquid valve 7_2 corresponds to ouput 2 of the valve number 7 (see

attached pneumatic diagram).




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2.3. Hydraulic cycle description

2.3.1. Atmosphere circuit

2.3.2. Diluent circuit

2.3.3. Clean circuit

2.3.4. Lyse circuit

2.3.5. WBC/RBC counting circuit

2.3.6. Drain/bubbling circuit



R A A 0 0 9

A i n d

B

ATMO SPHERE C IRCUIT

EAC 017 A



R A A 0 0 9

A i n d

B

DILUENT CIRCUIT

CO UNTING HE A D R INS E C IRCUIT

INNE R NE E DL E C IRCUIT

O UTE R NE E DL E R INS E C IRCUIT



R A A 0 0 9

A i n d

B

CLEAN CIRCUIT



R A A 0 0 9

A i n d

B

LYSE CIRCUIT



R A A 0 0 9

A i n d

B

WBC / RBC COUNTING PHASIS



R A A 0 0 9

A i n d

B

DRAIN / BUBBLING CIRCUIT



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3.1. With bottles




STRAIGHT CONNECTOR EAB015A 1.5/2.5 3



TYGON TUBE 0.040" EAE005A 1.02 110 1

TYGON TUBE 0.040" EAE005A 1.02 335 1

TYGON TUBE 0.060" EAE007A 1.52 15 3

TYGON TUBE 0.060" EAE007A 1.52 20 2

TYGON TUBE 0.060" EAE007A 1.52 40 4


TYGON TUBE 0.060" EAE007A 1.52 70 2

TYGON TUBE 0.060" EAE007A 1.52 80 1

TYGON TUBE 0.060" EAE007A 1.52 100 1

TYGON TUBE 0.060" EAE007A 1.52 150 1

TYGON TUBE 0.060" EAE007A 1.52 170 1

TYGON TUBE 0.060" EAE007A 1.52 220 1

TYGON TUBE 0.060" EAE007A 1.52 240 1

TYGON TUBE 0.060" EAE007A 1.52 420 2

TYGON TUBE 0.060" EAE007A 1.52 450 2

TYGON TUBE 0.060" EAE007A 1.52 480 1

TYGON TUBE 0.081" EAE008A 2.05 20 1

TYGON TUBE 0.081" EAE008A 2.05 35 1

TYGON TUBE 0.081" EAE008A 2.05 200 1

TYGON TUBE 0.081" EAE008A 2.05 330 1

TYGON TUBE 0.081" EAE008A 2.05 650 1

TYGON TUBE 0.081" EAE008A 2.05 1080 1

TYGON TUBE 0.090" EAE009A 2.28 20 2

TYGON TUBE 0.090" EAE009A 2.28 50 1

TYGON TUBE 0.090" EAE009A 2.28 60 3

TYGON TUBE 0.090" EAE009A 2.28 120 1

TYGON TUBE 0.090" EAE009A 2.28 140 1


SILICONE TUBE EAE025A 1.5/3.5 50 2

BLUE TYGON TUBE 0.090" EAE036A 2.28 1100 1

SLEEVE GAL098A 32



3. MICROS 60 CT HYDRAULIC



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AIR





Liquid valve 3_1 1.52 40 T connector 2.3_1

DILUENT

Diluent input 2.05 330 Liquid valve 11_1

Liquid valve 11_3 Y 1.52 220 temp sensor. xba281a Y

temp sensor. xba281a Y 1.52 40 liquid syringes_3 Y




Liquid valve 9_1 Y 1.52 420 Needle rinsing block._2


T connector 1.5_1 S1.5/3.5 50 Needle rinsing block._3

T connector 1.5_3 S1.5/3.5 50 Needle rinsing block._4

Liquid valve 7_2 Y 1.52 240 T connector 2.3_1T connector 2.3_2 1.52 50 WBC/HGB chamber_3

Liquid valve 10_2 Y 1.52 15 Connector 1.5/2.5

Connector 1.5/2.5 1.02 110 Connector 1.5/2.5

Connector 1.5/2.5 1.52 15 liquid syringes_1 Y

liquid syringes_2 Y 1.52 15 Connector 1.5/2.5

Connector 1.5/2.5 1.02 335 needle_1

Connector 1.5/2.5 Y needle_1 Y

Needle rinsing block._1 1.52 100 T connector 2.3_3

T connector 2.3_2 1.52 20 Liquid valve 8_1

Liquid valve 8_2 2.05 650 Waste-chamb._3

CLEANClean bottle 2.28 1100 blue Liquid valve 4_2 Y


LYSE

Lyse bottle 2.05 1080 Liquid valve 1_1Liquid valve 1_3 1.52 150 liquid syringes_4

Liquid valve 1_2 1.52 480 WBC grounding connector


WBC/RBC

counting

WBC/HGB chamber _2 1.52 170 RBC chamber_3

RBC chamber_2 1.52 450 Liquid valve 6_2 Y


DRAIN / BUBBLING

WBC/HGB Chamber_1 1.52 40 T connector 1.5_2

WBC/HGB Chamber_1 gbc088a 30 T connector 1.5_2

T connector 1.5_3 1.52 80 insulator WBC_1

insulator WBC_2 2.28 120 Liquid valve 12_2 Y


RBC_1 chamber 1.52 70 insulator RBC_1

RBC_1 chamber gbc088a 60 insulator RBC_1insulator RBC_2 2.28 150 Liquid valve 13_2 Y



T connector 2.3_3 2.28 20 Cell xba199a Y


T connector 2.3_1 2.28 60 Liquid valve 5_2 Y

Liquid valve 5_1 2.28 140 Waste ouput

NOTE Read this table as follows in this example :The Liquid valve 7_2 corresponds to ouput 2 of the valve number 7 (seeattached pneumatic diagram).




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3.2. Pack




STRAIGHT CONNECTOR EAB015A 1.5/2.5 3



TYGON TUBE 0.040" EAE005A 1.02 110 1

TYGON TUBE 0.040" EAE005A 1.02 335 1

TYGON TUBE 0.060" EAE007A 1.52 15 3

TYGON TUBE 0.060" EAE007A 1.52 20 2

TYGON TUBE 0.060" EAE007A 1.52 40 4


TYGON TUBE 0.060" EAE007A 1.52 70 2

TYGON TUBE 0.060" EAE007A 1.52 80 1

TYGON TUBE 0.060" EAE007A 1.52 100 1

TYGON TUBE 0.060" EAE007A 1.52 150 1

TYGON TUBE 0.060" EAE007A 1.52 170 1

TYGON TUBE 0.060" EAE007A 1.52 220 1

TYGON TUBE 0.060" EAE007A 1.52 240 1

TYGON TUBE 0.060" EAE007A 1.52 420 2

TYGON TUBE 0.060" EAE007A 1.52 450 2

TYGON TUBE 0.060" EAE007A 1.52 480 1

TYGON TUBE 0.081" EAE008A 2.05 20 1

TYGON TUBE 0.081" EAE008A 2.05 35 1

TYGON TUBE 0.081" EAE008A 2.05 200 1

TYGON TUBE 0.081" EAE008A 2.05 590 1

TYGON TUBE 0.081" EAE008A 2.05 650 1

TYGON TUBE 0.090" EAE009A 2.28 20 2

TYGON TUBE 0.090" EAE009A 2.28 50 1

TYGON TUBE 0.090" EAE009A 2.28 60 3

TYGON TUBE 0.090" EAE009A 2.28 120 1

TYGON TUBE 0.090" EAE009A 2.28 150 1

TYGON TUBE 0.090" EAE009A 2.28 190 1

TYGON TUBE 0.090" EAE009A 2.28 510 1

TYGON TUBE 0.090" EAE009A 2.28 550 1

TYGON TUBE 0.090" EAE009A 2.28 1100 1

SILICONE TUBE EAE025A 1.5/3.5 50 2

SLEEVE GAL098A 33



METALLIC SHEATH GBC170A 5.2 35 3



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AIR




(atmosphere) 2.28 60 Liquid valve 3_2Liquid valve 3_1 1.52 40 T Connector 2.3_1

DILUENT

Pack_3 (Diluent) 2.28 550 Liquid valve 11_1 Y

Pack_3 (Diluent) gbc170a

Liquid valve 11_3 Y 1.52 220 Temp. sensor xba281a Y

Temp. sensor xba281a Y 1.52 40 Liquid syringes_3 Y




Liquid valve 9_1 Y 1.52 420 Needle rinsing block_2

Liquid valve 9_2 Y 1.52 420 T Connector 1.5_2

T Connector 1.5_1 S1.5/3.5 50 Needle rinsing block_3

T Connector 1.5_3 S1.5/3.5 50 Needle rinsing block_4

Liquid valve 7_2 Y 1.52 240 T Connector 2.3_1T Connector 2.3_2 1.52 50 Bac WBC/HGB_3

Liquid valve 10_2 Y 1.52 15 connector1.5/2.5

connector1.5/2.5 1.02 110 connector1.5/2.5

connector 1.5/2.5 1.52 15 Liquid syringes_1 Y

Liquid syringes_2 Y 1.52 15 connector 1.5/2.5

connector 1.5/2.5 1.02 335 Needle_1

connector 1.5/2.5 Y Needle_1 Y

Needle rinsing block_1 1.52 100 T Connector 2.3_3

T Connector 2.3_2 1.52 20 Liquid valve 8_1

Liquid valve 8_2 2.05 650 Waste-chamb._3

CLEAN

Pack_1 (Clean) 2.28 510 Liquid valve 4_2 Y

Pack_1 (Clean) gbc170a


LYSE

Pack_2 (Lyse) 2.05 590 Liquid valve 1_1

Pack_2 (Lyse) gbc170a

Liquid valve 1_3 1.52 150 Liquid syringes_4

Liquid valve 1_2 1.52 480 WBCgrounding connec.

WBC grounding connect. 1.52 20 T Connector 1.5_1

Comptage

WBC/RBC

Bac WBC/HGB_2 1.52 170 RBC chamber_3

RBC chamber_2 1.52 450 Liquid valve 6_2 Y


EVACUAT°

/ Bullage

Bac WBC/HGB_1 1.52 40 T Connector 1.5_2

Bac WBC/HGB_1 gbc088a 30 T Connector 1.5_2

T Connector 1.5_3 1.52 80 insulator WBC_1

insulator WBC_2 2.28 120 Liquid valve 12_2 Y


RBC chamber_1 1.52 70 insulator RBC_1

RBC chamber_1 gbc088a 60 insulator RBC_1

insulator RBC_2 2.28 150 Liquid valve 13_2 Y


T Connector 2.3_3 2.05 200 T Connector 2.3_2

T Connector 2.3_3 2.28 20 CELL xba199a Y

CELL xba199a Y 2.28 60 Waste-chamb._5 Y

T Connector 2.3_1 2.28 60 Liquid valve 5_2 Y

Liquid valve 5_1 2.28 1100 Pack_4 (Waste)

NOTE Read this table as follows in this example :

The Liquid valve 7_2 corresponds to ouput 2 of the valve number 7 (seeattached pneumatic diagram)




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3.3. Hydraulic cycle description

3.3.1. Atmosphere circuit

3.3.2. Diluent circuit

3.3.3. Clean circuit

3.3.4. Lyse circuit

3.3.5. WBC/RBC counting circuit

3.3.6. Drain/bubbling circuit



R A A 0 0 9

A

i n d

B

ATMO SPHERE C IRCUIT

EAC 017 A

ATM O SPHERE

ATM O SPHERE



R A A 0 0 9

A i n d

B

DILUENT CIRCUIT

C O U N T I N G H E A D R I N S E C I R C U I T

S A M P L I N G N E E D L E I N N E R C I R C U I T

O U T E R S A M P L I N G N E E D L E R I N S E C I R C U I T

P I E R C I N G N E E D L E R I N S E C I R C U I T



R A A 0 0 9

A

i n d

B

CLEAN CIRCUIT



R A A 0 0 9

A i n d

B

LYSE CIRCUIT



R A A 0 0 9

A

i n d

B

WBC / RBC COUNTING CIRCUIT



R A A 0 0 9

A i n d

B

DRAIN / BUBBLING CIRCUIT



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4. PNEUMATIC DIAGRAMS

4.1. Micros 60 CT bottle version

4.2. Micros 60 CT pack version

4.3. Micros 60 OT bottle version

4.4. Micros 60 OT pack version



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CONTENTS

1. POWER SUPPLY ASSEMBLY .............................................................................................................. 2

1.1. Principle ......................................................................................................................................... 21.2. Power supply fan ............................................................................................................................ 41.3. Secondary supply cable ................................................................................................................. 51.4. Main supply filter ............................................................................................................................ 6

2. MOTHER BOARD .................................................................................................................................. 7

2.1. Configuration .................................................................................................................................. 7

3. LCD BOARD .......................................................................................................................................... 8

4. COAXIALS ............................................................................................................................................. 94.1. RBC coaxial ................................................................................................................................... 94.2. WBC coaxial .................................................................................................................................11

5. SENSORS............................................................................................................................................ 12

5.1. Drain detection sensor ................................................................................................................. 125.2. Carriage & needle sensors ........................................................................................................... 135.3. Syringe sensors ............................................................................................................................ 14

6. MISCELLANEOUS............................................................................................................................... 15

6.1. Needle carriage motor.................................................................................................................. 15

6.2. Barcode cable .............................................................................................................................. 166.3. Temperature sensor ..................................................................................................................... 176.4. HGB Chamber assembly ............................................................................................................. 186.5. Chip card reader cable ................................................................................................................. 196.6. CT Piercing assembly cable......................................................................................................... 206.7. CT twin valve cable...................................................................................................................... 21

7. FLAT CABLES ..................................................................................................................................... 22

8. ELECTRICAL SYNOPTICS ................................................................................................................. 26



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Page 2/2 30/06/98

1. POWER SUPPLY ASSEMBLY

1.1. Principle



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Page 2/4 30/06/98

1.2. Power supply fan



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1.3. Secondary supply cable



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Page 2/6 30/06/98

1.4. Main supply filter



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2. MOTHER BOARD

2.1. Configuration



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Page 2/8 30/06/98

3. LCD BOARD



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4. COAXIALS

4.1. RBC coaxial



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4.2. WBC coaxial



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5. SENSORS

5.1. Drain detection sensor



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5.2. Carriage & needle sensors



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5.3. Syringe sensors



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6. MISCELLANEOUS

6.1. Needle carriage motor



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6.2. Barcode cable



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6.3. Temperature sensor



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Page 2/18 30/06/98

6.4. HGB Chamber assembly



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6.5. Chip card reader cable



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Page 2/20 30/06/98

6.6. CT Piercing assembly cable



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6.7. CT twin valve cable



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7. FLAT CABLES



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Page 2/24 30/06/98



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8. ELECTRICAL SYNOPTICS



RAA 009 A Ind.A Page 3/1 20/06/98

3. M A I N T E N A N C E

CONTENTS

1. MAINTENANCE ..................................................................................................................................... 2

1.1. Introduction .................................................................................................................................... 21.2. Daily customer maintenance.......................................................................................................... 21.3. Weekly customer maintenance...................................................................................................... 2

2. Maintenance kits .................................................................................................................................... 3

3. PROCEDURES ...................................................................................................................................... 4

3.1. Procedure chart tables ................................................................................................................... 53.2. Required tools and products ............................................................................................... ........... 7



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Page 3/2 20/06/98

1. MAINTENANCE

1.1. Introduction

1.2. Daily customer maintenanceNo special adjustments or maintenance has to be done on your equipment if the recommendedstartup and shutdown procedures are explicitly respected.See the ABX MICROS 60 User Manual for the daily rinsing and cleaning of the system.

1.3. Weekly customer maintenance

An overall check for cleanliness of the system is recommended every week.All traces of blood or reagent have to be wiped off as soon as possible using a piece of clothand distilled water.

WARNING ! Customer maintenance has to be carried out according to the recommendedfrequency chart table and after having performed an ABX approved customer trai-ning course.The system warranty may be affected if damage occurs after a non trained technicianintervenes or if replaced spare parts and consumables do not come from an ABXapproved origin.

CAUTION Never use solvent or abrasive cleaning material to clean the system.



RAA 009 A Ind.A Page 3/3 20/06/98


2. MAINTENANCE KITS

P a r t n u m b e rS P A R E P A R T S K I T

X E A 4 5 8 A SQ u a n t i t y

CAE 006 A tube holder detection switch 1

EAE 005 A Tygon tube 1.02mm (0,040") 2

EAE 007 A Tygon tube 1,52mm (0,060") 2

EAE 008 A Tygon tube 2,05mm (0,081") 2EAE 009 A Tygon tube 2,29mm (0,090") 2

FAK 001 A Aperture 50µ 1

FAK 003 A Aperture 80µ 1

FBR 002 A Notched belt 290 1

FBR 003 A Notched belt 380 1

GBC 052 A C.T sampling needle 1

GBC 069 A O.T sampling needle 1

GBC 189 A CT Piercing needle 1

GBC 193 A RBC chamber 1

XBA 199 A Drain detection sensor 1

XBA 250 A Carriage/needle sensor 1

XBA 272 B WBC coaxial 1

XDA 472 B WBC/HGB chamber 1

XDA 481 B Liquid valve 2W NC without solenoid 1

XDA 483 B Liquid valve 3W without solenoid 1

XBA 365 A RBC coaxial 1

XBA 319 A Liquid/air syringe sensor 1

XEA 328 AS Maintenance kit 1

O R I N G K I T : X E A 3 2 8 A S

FAA 036 A1 Lyse dispenser O ring1 Piercing needle O ring (MICROS CT only)

FAA 029 A 1 Diluent dispenser O ring

FAA 055 A 2 Sampling syringe O rings

FAA 049 A 2 Aperture O rings

FAA 046 A 2 Coaxial O rings

FAA 054 A 2 Sampling needle O rings (CT)

FAA 053 A 1 Sampling needle O ring (OT)

FAA 017 A 1 Vacuum/waste syringe O ring

KAM 022 A 6 Board holder clips (MICROS 45 only)

FAA 023 A 1 WBC/HGB chamber cap O ring

GBC 030 A 1 Air Syringe piston



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Page 3/4 20/06/98

3. PROCEDURES

CONCERNING PARTS :

- Hydraulic maintenance and adjustments.

- Pneumatic maintenance and adjustments.

- Electrical maintenance and adjustments.

- Power supply maintenance and adjustments.

- Electronic maintenance and adjustments.

- Printer maintenance and setup.

NOTE Maintenance and adjustments that need to be done on ABX MICROS 60

are divided into «procedures» according to concerned assemblies. Thisshould make any updating easier as all interventions can be done with thecorresponding «procedure» on its own.

NOTE Each procedure has to be read entirely before beginning the intervention.

WARNING ! When cleaning instruments, disposable gloves should be worn.



RAA 009 A Ind.A Page 3/5 20/06/98


3.1. Procedure chart tables

M I C R O S 6 0 O T

P / N P R O C E D U R E C O N C E R N S

RAS 165 AMICROS 60 OT INSTALLATION

AND STARTUP

Unpacking - Working conditions - Visual checks - Reagent

connection - Printer & instrument connections - Priming &

Startup

RAS 168 ASAMPLING NEEDLE

MAINTENANCE MICROS OTNeedle replacement - O ring replacement

RAS 169 A CHAMBER MAINTENANCERBC, WBC/HGB chamber cleaning - Aperture O ring

replacement - Coaxial O ring replacement

RAS 170 A LIQUID VALVE MAINTENANCE Liquid valve assy replacement - Valve body replacement

RAS 171 APOWER SUPPLY

CHECK/REPLACEMENT

Voltage supply check - Power supply module replacement

Fan operation check.

RAS 172 ATECHNICIAN FUNCTIONS

MICROS 60 OT

Version display. - Adjustments : HGB photometer calibration,

Aperture voltage, pressure check, WBC gain , RBC & PLT

gain, Sensor, Needle heigth and motion, bubbling. -

Temperature sensor adjustment - Run mode - Reagent pack -

Serial number - Cycle number - Burning

RAS 173 A MECHANIC FUNCTIONS

Sensor replacement - Needle motion check - Carriage motion

check - Liquid syringe motion check - Vac/Waste syringe

motion check - Valve operation check - LCD contrast - Piercing

mechanism check

RAS 174 A DRAIN DETECTION ADJUSTMENT Drain detection sensor adjustment

RAS 175 A PCB VOLTAGE CHECKS

Voltage supply check/adj. - Aperture voltage check/adj. -

RBC threshold check/adj. - WBC threshold check/adj. - PLT

threshold check/adj. - HGB blank voltage check - Stepper

motor voltage adjustment

RAS 177 A LX300 PRINTERConfiguration - Control panel - Control LEds and keys -

Description

RAS 178 A LIQUID SYRINGE MAINTENANCE

Lyse dispenser O ring replacement - Diluent dispenser O ring

replacement - Sampling needle dispenser O ring replact. -

Lubrification

RAS 179 AVACUUM/WASTE SYRINGE

MAINTENANCEO ring replacement

RAS 180 A CHANGING THE INSTRUMENTLANGUAGE

Changing the instrument language

RAS 181 A REAGENT PACK Connector O ring replacement

RAS 182 A BARCODE READER SETUP Reader configuration

RAS 187 AHYDRAULIC CYCLE CHECKUP

MICROS 60 OTStep by step control of the hydraulic cycle

RAS 191 A OVERALL MAINTENANCE Instrument maintenance step by step

RAS 192 A DECONTAMINATION Instrument decontamination

RAS 197 A DRAIN & RINSE Instrument rinse and drain for an extended shutdown



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Page 3/6 20/06/98

M I C R O S 6 0 C T

P / N P R O C E D U R E C O N C E R N S

RAS 166 AMICROS 60 CT INSTALLATION

AND STARTUP

Unpacking - Working conditions - Visual checks - Reagentconnection - Printer & instrument connections - Priming &

Startup

RAS 167 ASAMPLING NEEDLE

MAINTENANCE MICROS CT

Needle O ring replacement - Sampling needle replacement

Piercing needle replacement

RAS 169 A CHAMBER MAINTENANCERBC, WBC/HGB chamber cleaning - Aperture O ring

replacement - Coaxial O ring replacement

RAS 170 A LIQUID VALVE MAINTENANCE Liquid valve assy replacement - Valve body replacement

RAS 171 APOWER SUPPLY

CHECK/REPLACEMENT

Voltage supply check - Power supply module replacement

Fan operation check.

RAS 176 ATECHNICIAN FUNCTIONS

MICROS 60 CT

Version display. - Adjustments : HGB photometer calibration,Aperture voltage, pressure check, WBC gain , RBC & PLT

gain, Sensor, Needle heigth and motion, bubbling. -

Temperature sensor adjustment - Run mode - Reagent pack -

Serial number - Cycle number - Burning

RAS 173 A MECHANIC FUNCTIONS

Sensor replacement - Needle motion check - Carriage motion

check - Liquid syringe motion check - Vac/Waste syringe

motion check - Valve operation check - LCD contrast - Piercing

mechanism check

RAS 174 A DRAIN DETECTION ADJUSTMENT Drain detection sensor adjustment

RAS 175 A PCB VOLTAGE CHECKS

Voltage supply check/adj. - Aperture voltage check/adj. -

RBC threshold check/adj. - WBC threshold check/adj. - PLT

threshold check/adj. - HGB blank voltage check - Steppermotor voltage adjustment

RAS 177 A LX300 PRINTERConfiguration - Control panel - Control LEds and keys -

Description

RAS 178 A LIQUID SYRINGE MAINTENANCE

Lyse dispenser O ring replacement - Diluent dispenser O ring

replacement - Sampling needle dispenser O ring replact. -

Lubrification

RAS 179 AVACUUM/WASTE SYRINGE

MAINTENANCEO ring replacement

RAS 180 ACHANGING THE INSTRUMENT

LANGUAGEChanging the instrument language

RAS 181 A REAGENT PACK Connector O ring replacement

RAS 182 A BARCODE READER SETUP Reader configuration

RAS 188 AHYDRAULIC CYCLE CHECKUP

MICROS 60 CTStep by step control of the hydraulic cycle

RAS 191 A OVERALL MAINTENANCE Instrument maintenance step by step

RAS 192 A DECONTAMINATION Instrument decontamination

RAS 197 A DRAIN & RINSE Instrument rinse and drain for an extended shutdown

RAS 198 A PIERCING BLOCK Description - Maintenance - Sampling position



RAA 009 A Ind.A Page 3/7 20/06/98


3.2. Required tools and products

T O O L S P R O D U C T S

DESIGNATION PART NUMBER DESIGNATION PART NUMBER

HEXAGONAL KEYSEMPTY SAMPLE

TUBES

DYNAMOMETRIC

SCREW DRIVER

A302

MAG 019 A SILICONE GREASE LAM 004 A

DYNAMOMETRIC

SCREW DRIVER

A301

MAG 020 A

GREASE FOR

MECHANICAL

ASSEMBLIES

XEA 381 AS

DYNAMOMETRICSCREW DRIVER

A300

MAG 013 A SOFT TISSUE

CLAMPS LIQUID SOAP

SCALPEL DISTILLED WATER

CUTTING PLIERS MICROPIPETTE TIP

PAIR OF SCISORSFLAT PIECE OF

STIFF PLASTIC

VOLTMETER LATEX WBC LAD 001 AS

FLAT SCREWDRIVER LATEX RBC LAD 002 AS

BARFLEX FELT PEN

THERMOMETER SYRINGE 5ML

TORX KEYS



REQUIRED PRODUCTS

M

I C

R O S

6 0 O

T

RAS 165 A Ind.B

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY

SPECIFIC KIT OR CONSUMABLES

INSTALLATION

01/10/98

- PACK installation kit : XEA 314 Aor

- Bottle installation kit : XEA 332 A

- 30 minutes

- MICROS 60 Reagents : Bottles or Pack.- Waste container (for bottle model).

1 - Unpacking

2 - Working conditions3 - Visual checks4 - Reagent connections5 - Printer and instrument connections6 - Priming and startup

Hexagonal keys



M

I C R O S

6 0 O

TINSTALLATION

Page 2/13 RAS 165 A Ind.B

PROCEDURE

The instrument is enveloped in a special,protective foam before being placed in acardboard box. Cut the four angles of the boxto unpack the system.Remove the cardboard box containing theABXMICROS 60 installation kit from its location (seeDiag.1).

Diag.1

1 - Unpacking

2 - Working conditions

• EnvironmentABX MICROS 60-OT should be operated in an indoor location only. Operation at an altitudeover 2000 meters is not recommended. Instrument is designed to be safe for transient volta-ges according to INSTALLATION CATEGORY II and POLLUTION DEGREE 2.

• LocationABX MICROS 60-OT should be placed on a clean and level table or work station. Please notethat ABX MICROS 60-OT , printer and reagents weigh approximately 30 kilograms (66 lbs).Avoid exposure to sunlight. Proper ventilation requires that a space of at least 20 cm (8 inches)must be left behind the apparatus.

• GroundingProper grounding is required. Check that the wall ground (earth) plug is correctly connected tothe laboratory grounding electricity installation. If there is no ground then use a ground stake.

Current electricity norms must be applied.

• Humidity and temperature conditionsABX MICROS 60-OT can function between 18 to 32°C (65 to 90°F), with relative humidity,meaning less than 80% with no condensation. If it is kept at a temperature less than 10°C(50°F), the instrument should be allowed to sit for an hour at the correct room temperaturebefore use.



INSTALLATION M I C

R O

S 6

0 O T

RAS 165 A Ind.B Page 3/13

A

B

Diag.3

3 - Visual checks

Push the black plastic carriage locking clip(A) as far as possible to the left and placethe sample needle carriage (B) as farforward as possible to the right-hand side,as shown in Diag. 5.Check that the needle is not bent and makesure it is in its upper position.

Using the key contained into the installation kit, turnthe locker as shown on the Diag. 2 to open thepneumatic protection door.

Unscrew the 3 cover fixation screws (Diag. 3) andloosen the 2 tightening screws under the reagentflap (Diag 4).Remove the cover : pull it backward and lift it up tothe rear of the instrument .

Diag.2

Diag.4

Diag.5



M

I C R O S

6 0 O

TINSTALLATION


Unscrew slightly the 2 screws of the WBC/HGB chamber protection cover (diag 6). Removethe cover and check that both chambers (RBC/PLT, WBC/HGB) are fixed properly in their clipsand the electrode blocks are attached firmly to the chambers (Diag. 7).

Diag.7

Diag.6

Re-install the HGB/WBC chamber cover.

Diag.8

Check that the connectors on the

printed circuit board are securely inplace (Diag. 8).

Re-install the instrument cover.

Remove the fuse holder from its location onthe rear panel pressing on the holder lock(Diag.9) and check the fuse characteristics :they should be 1 Ampere, 250 Volts Slow-Blow.

Diag.9



INSTALLATION M I C

R O

S 6

0 O T


4 - Reagent connections

• Bottle connections

Lyse and cleaning reagents are placed inside the

reagent compartment as shown in the Diag. 10.Install the reagent straws and the bottle stoppers.Connect the blue tube to the MINICLEAN bottleand the white tube to the MINILYSE bottle.Close the compartment cover.

Diag.10

IMPORTANT When the ABX MICROS 60-OT is set up with the 16 or 18 parameters mode, it is mandatoryto use specific MINILYSE LMG and MINIDIL LMG reagents.

CAUTION The Diluent container will be located on the bench at the same level than the instru-ment.

Diag.11

Install the male connectors included in theinstallation kit at the liquid input and out-put located at the bottom of the instru-ment rear panel (Diag.11).

Connect the diluent container (see CAU-TION above) using the diluent straw anda 3x6 cristal tube (1 meter maximum) on

the diluent input located at the bottom ofthe instrument rear panel (Diag.11).Connect the waste container using thecristal tube 3 x 6 on the waste output,and place the waste container below theinstrument level (under the bench).

• Waste connection

WARNING ! Always follow the recommended procedures for waste disposal. Never connectthe instrument wastes directly to the laboratory drain pipes. For each waste con-tainer, follow the neutralization procedure as described in the user manal.



M

I C R O S

6 0 O

TINSTALLATION


• Reagent pack connectionRemove the reagent output protections, as well as the waste input protection (Diag.12 & 13)

Install the pack directly into the compartment of the instrument as shown on the Diag. 14, 15 &16. Push the pack down in order to plug correctly the pack on the male connectors.

The free male connector (see Diags.

14, 15 & 16) must be plugged on thepack upper valve in order to receivethe waste liquids.

Diag.12 Diag.13

Diag.14 Diag.15

Diag.16

CAUTION In order to avoid leak problems it is recommended not to unplug several times thesame reagent pack.



INSTALLATION M I C

R O

S 6

0 O T


• Setting up the printer- Remove all the package protections- Install the paper feed-knob

Diag.17

Diag.18

Open the ink ribbon access door at the top of the printer and install the ribbon as shown in diag20 & 21 : Slide the printer head to the middle of the printer.

Insert the ribbon cartridge into the printerGuide the ribbon between the print head and ribbon guide.Slide the printer head from side to side to make sure it moves smoothly.

Diag.19

Diag.20

5 - Printer and instrument connections

• Instrument connection.

Diag.21

Connect the power cable to the plug locatedon the rear left-hand side of the device (Diag.17).

If the instrument has to be connected to alaboratory computer, use the plug RS232.



M

I C R O S

6 0 O

TINSTALLATION


- The printer is connected to ABX MICROS 60-OT with the cable delivered with the instrument.

Lock the connector in place by tightening the 2 screws on each end of the connector to theABX MICROS 60-OT . Attach the other end of the cable to the printer and lock the printerconnector in place by the means of the 2 clips located on the connector itself (Diag. 23).

Diag.23

Diag.22

Diag.24

- Install the paper supports (Diag 22) for single sheets paper use only.

- For continuous paper, introduce it in the slot at the back of the printer and use the sprocketcovers to load paper, or else feed the paper frontward when using single sheets (see printeruser manual).

Diag.25

INSTALLATION M I C

R O

S 6

0 O T


• Printer command keys

• Printer ConfigurationSwitch on the printer when pressing the . The configuration should be the following :

Key LF/FF : During normal operation, a quick pressure on this key allows a ligne feed of thepaper. Keep the pressure on this key to feed a whole page. This key can be used to load oreject the paper.

When this key is pressed during the printer power ON, the printing test starts. Key PAUSE :

When this key is pressed during the printing, the printout stops. Press again on this key torestart the printout.

Key FONT : During normal operation, the FONT key allows the font selection. For each pres-sure on this key, the selection is modified. Refer to the printer user's manual to select the font.When this key is pressed during the printer power ON, the printer setup menu is entered.

LED PAUSE : The orange LED PAUSE lights when the printer stops printing. During eachpower ON, this LED blinks for few seconds and 4 audible beeps occur. When the printer runsout of paper, the LED blinks and 3 audible beeps occur. This LED lights also when the paper isin its tear off position. When a problem occurs, this LED lights ON and 5 audible beeps occur.

LEDS FONT 1 and FONT 2 : These 2 green LEDS indicate the selected font. Refer to theprinter user's manual to select the font.

Diag.26

PAPER PARK : If Z folded paper is used, the paper can be driven to its parking position whenpressing simultaneously on the keys LF/FF and FONT.

MICRO ADJUST : This function allows to adjust the loading paper position. See the user'smanual for details.

Diag.29

M

I C R O S

6 0 O

TINSTALLATION


Each parameter can be modified by the corresponding parameter chart.Each chart is accessible using the keys <PAUSE>, and <LF/FF> according to thecontrol LED combinations (Diag 30).

6 - Instrument startup

• Reagent primingWhen the ABX MICROS 60-OT is first installed, it contains no reagents. All the reagents haveto be primed now. Turn ON instrument by pressing the ON/OFF switch located on the rearpanel. When the instrument turns on, the display shows :

PLEASE WAIT FOR 3 MINESCAPE : ESC

STARTUP NOT INITIATEDPRESS A KEY TO CONTINUE...

This time is required at the startup for the instrument initialization and stabilization, specificallyfor the HGB diode to reach its operationnal temperature. Press ESC several times in order toabort the cycle : the LED of the front panel turns from red to green and the display shows thefollowing :

This message appears when the instrument is setup with the manual startup cycle to preventany analysis cycle before running a startup cycle.Press any key, the main menu is displayed :

MAIN MENU 1 RESULTSHH : MM 2 QC

Diag.30



INSTALLATION M I C

R O

S 6

0 O T


• Bottles and containers set :

Install the reagent bottle and carry out a PRIME cycle to clear the reagent line of air bubbles.This procedure should be done whenever a new bottle of reagent is installed.

From the MAIN MENU, move the cursor to the function 4 SERVICE and press ENTER. The

service menu is displayed :

SERVICE 1 BACK FLUSHHH : MM 2 DRAIN CHAMBERS

Move the cursor to function 3 PRIME REAGENTS and press ENTER. The PRIME menu is

displayed :

PRIME 1 ALL REAGENTS

HH : MM 2 DILUENT

Select either the function 1 prime ALL REAGENTS or move the cursor next to the required

reagent and press ENTER.

The priming cycle starts while the following menu is displayed :

ALL REAGENTS WAIT FOR 2 MN 3 S******************

IMPORTANT Before analyzing samples, visually inspect reagent lines and pumps for air bubbles.Repeat priming if air bubbles are still present. Call the ABX representative service department if priming does not eliminate air bubbles.

Never initiate two Lyse prime cycles back-to-back. This causes excessive foaming inthe waste chamber. Run a blank cycle between each Lyse prime cycle.

- Run a STARTUP cycle.

M

I C R O S

6 0 O

TINSTALLATION


IMPORTANT

From the REAGENT PACK menu, the function 2 "CBC LEFT" displays the number of analysis

cycles left to run with the same pack.

It is also possible to run a priming cycle at any time using the selection 3 "PRIME REAGENTS"

of the SERVICE menu.

Once the new PACK is installed a priming cycle will be automatically carried out and thefollowing menu is displayed.

PRIME WAIT FOR 2 MIN 3 S* * * * * * * * * *

Move the cursor to 3 PRIME REAGENTS and press the ENTER key. Select the function

1 CHANGE PACK and follow the instructions given by the LCD in order to install the pack.

REAGENT PACK > 1 CHANGE PACKHH : MM 2 CBC LEFT < 150>

• Reagent pack



SERVICE > 2 DRAIN CHAMBER

HH : MM 3 PRIME REAGENTS

IMPORTANT Before analyzing samples, visually inspect reagent lines and pumps for air bubbles.

Repeat priming if air bubbles are still present. Call the ABX representative service department if priming does not eliminate air bubbles.

It is recommended not to remove the pack several times before the reagents aretotally used in order to avoid leak problems.



INSTALLATION M I C

R O

S 6

0 O T


Once the instrument ready for the analyses, remove the adhesive protection from the frontpanel (diag 31)

Diag.31



REQUIRED PRODUCTS

M I C

R O S 6 0 C T

RAS 166 A Ind.B

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


INSTALLATION

01/10/98

PACK installation kit : XEA 317 A orBottle installation kit : XEA 335 A or

30 minutes

Reagents : Bottles or Pack.Waste container (for bottle model).

1 - Unpacking

2 - Working conditions3 - Visual checks4 - Reagent connections5 - Printer and instrument connections6 - Priming and startup

Hexagonal keys



M

I C

R O S 6

0 C

TINSTALLATION


PROCEDURE

The instrument is enveloped in a special,protective foam before being placed in acardboard box. Cut the four angles of the boxto unpack the system.Remove the cardboard box containing the ins-trument installation kit from its location (seeDiag.1).

Diag.1

1 - Unpacking

2 - Working conditions

• EnvironmentABX MICROS 60-CT should be operated in an indoor location only. Operation at an altitudeover 2000 meters is not recommended. Instrument is designed to be safe for transient volta-ges according to INSTALLATION CATEGORY II and POLLUTION DEGREE 2.

• LocationABX MICROS 60-CT should be placed on a clean and level table or work station. Please notethat ABX MICROS 60-CT , printer and reagents weigh approximately 30 kilograms (66 lbs).Avoid exposure to sunlight. Proper ventilation requires that a space of at least 20 cm (8 inches)must be left behind the apparatus.

• GroundingProper grounding is required. Check that the wall ground (earth) plug is correctly connected tothe laboratory grounding electricity installation. If there is no ground then use a ground stake.

Current electricity norms must be applied.

• Humidity and temperature conditionsABX MICROS 60-CT can function between 18 to 32°C (65 to 90°F), with relative humidity,meaning less than 80% with no condensation. If it is kept at a temperature less than 10°C(50°F), the instrument should be allowed to sit for an hour at the correct room temperaturebefore use.



INSTALLATION

M I C R O

S 6 0

C T RAS 166 A Ind.B Page 3/13

Diag.3

3 - Visual checks

Push the black plastic carriage locking clip(A) as far as possible to the left and placethe sample needle carriage (B) as farforward as possible to the right-hand side,as shown in Diag. 5.Check that the needle is not bent and makesure it is in its upper position.

Using the key contained into the installation kit, turnthe locker as shown on the Diag. 2 to open thepneumatic protection door.

Unscrew the 3 cover fixation screws (Diag. 3) andloosen the 2 tightening screws under the reagentflap (Diag 4).Remove the cover : pull it backward and lift it up tothe rear of the instrument.

Diag.2

Diag.4

Diag.5



M

I C

R O S 6

0 C

TINSTALLATION


Unscrew slightly the 2 screws of the WBC/HGB chamber protection cover (diag 6). Removethe cover and check that both chambers (RBC/PLT, WBC/HGB) are fixed properly in their clipsand the electrode blocks are attached firmly to the chambers (Diag. 7).

Diag.7 Diag.6

Re-install the HGB/WBC chamber cover.

Diag.8

Check that the connectors on the

printed circuit board are securely inplace (Diag. 8).

Re-install the instrument cover.

Remove the fuse holder from its location onthe rear panel pressing on the holder lock(Diag.9) and check the fuse characteristics :they should be 1 Ampere, 250 Volts Slow-Blow.

Diag.9



INSTALLATION

M I C R O

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4 - Reagent connections

• Bottle connections

Lyse and cleaning reagents are placed inside the

reagent compartment as shown in the Diag. 10.Install the reagent straws and the bottle stoppers.Connect the blue tube to the MINICLEAN bottleand the white tube to the MINILYSE bottle.Close the compartment cover.

Diag.10

IMPORTANT When the ABX MICROS 60-CT is set up with the 16 or 18 parameters mode, it is mandatoryto use specific MINILYSE LMG and MINIDIL LMG reagents.

CAUTION The Diluent container will be located on the bench at the same level than the instru-ment.

Diag.11

Install the male connectors included in theinstallation kit at the liquid input and out-put located at the bottom of the instru-ment rear panel (Diag.11).

Connect the diluent container (see CAU-TION above) using the diluent straw anda 3x6 cristal tube (1 meter maximum) on

the diluent input located at the bottom ofthe instrument rear panel (Diag.11).Connect the waste container using thecristal tube 3 x 6 on the waste output,and place the waste container below theinstrument level (under the bench).

• Waste connection

WARNING ! Always follow the recommended procedures for waste disposal. Never connectthe instrument wastes directly to the laboratory drain pipes. For each waste con-tainer, follow the neutralization procedure as described in the user manal.



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• Reagent pack connectionRemove the reagent output protections, as well as the waste input protection (Diag.12 & 13)

Install the pack directly into the compartment of the instrument as shown on the Diag. 14, 15 &16. Push the pack down in order to plug correctly the pack on the male connectors.

The free male connector (see Diags.

14, 15 & 16) must be plugged on thepack upper valve in order to receivethe waste liquids.

Diag.12 Diag.13

Diag.14 Diag.15

Diag.16

CAUTION In order to avoid leak problems it is recommended not to unplug several times thesame reagent pack.



INSTALLATION

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• Setting up the printer- Remove all the package protections- Install the paper feed-knob

Diag.17

Diag.18

Open the ink ribbon access door at the top of the printer and install the ribbon as shown in diag20 & 21 : Slide the printer head to the middle of the printer.

Insert the ribbon cartridge into the printerGuide the ribbon between the print head and ribbon guide.Slide the printer head from side to side to make sure it moves smoothly.

Diag.19

Diag.20

5 - Printer and instrument connections

• Instrument connection.

Diag.21

Connect the power cable to the plug locatedon the rear left-hand side of the device (Diag.17).

If the instrument has to be connected to alaboratory computer, use the plug RS232.



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- The printer is connected to ABX MICROS 60-CT with the cable delivered with the instrument.

Lock the connector in place by tightening the 2 screws on each end of the connector to theABX MICROS 60-CT . Attach the other end of the cable to the printer and lock the printerconnector in place by the means of the 2 clips located on the connector itself (Diag. 23).

Diag.23

Diag.22

Diag.24

- Install the paper supports (Diag 22) for single sheets paper use only.

- For continuous paper, introduce it in the slot at the back of the printer and use the sprocketcovers to load paper, or else feed the paper frontward when using single sheets (see printeruser manual).

Diag.25

INSTALLATION

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• Printer command keys

• Printer ConfigurationSwitch on the printer when pressing the . The configuration should be the following :

Key LF/FF : During normal operation, a quick pressure on this key allows a ligne feed of thepaper. Keep the pressure on this key to feed a whole page. This key can be used to load oreject the paper.

When this key is pressed during the printer power ON, the printing test starts. Key PAUSE :

When this key is pressed during the printing, the printout stops. Press again on this key torestart the printout.


LED PAUSE : The orange LED PAUSE lights when the printer stops printing. During eachpower ON, this LED blinks for few seconds and 4 audible beeps occur. When the printer runsout of paper, the LED blinks and 3 audible beeps occur. This LED lights also when the paper isin its tear off position. When a problem occurs, this LED lights ON and 5 audible beeps occur.


Diag.26



Diag.29

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Each parameter can be modified by the corresponding parameter chart.Each chart is accessible using the keys <PAUSE>, and <LF/FF> according to thecontrol LED combinations (Diag 30).

6 - Instrument startup

• Reagent primingWhen the ABX MICROS 60-CT is first installed, it contains no reagents. All the reagents haveto be primed now. Turn ON instrument by pressing the ON/OFF switch located on the rearpanel. When the instrument turns on, the display shows :

PLEASE WAIT FOR 3 MINESCAPE : ESC

STARTUP NOT INITIATED

PRESS A KEY TO CONTINUE...

This time is required at the startup for the instrument initialization and stabilization, specificallyfor the HGB diode to reach its operational temperature. Press the ESC key in order to abort thecycle : the LED of the front panel turns from red to green and the display shows :

This message appears when the instrument is setup with the manual startup cycle to preventany analysis cycle before running a startup cycle.Press any key, the main menu is displayed :

MAIN MENU 1 RESULTSHH : MM 2 QC

Diag.30



INSTALLATION

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Bottles and containers set :

Install the reagent bottle and carry out a PRIME cycle to clear the reagent line of air bubbles.This procedure should be done whenever a new bottle of reagent is installed.



SERVICE 1 BACK FLUSHHH : MM 2 DRAIN CHAMBERS

Move the cursor to function 3 PRIME REAGENTS and press ENTER. The PRIME menu is

displayed :

PRIME 1 ALL REAGENTS

HH : MM 2 DILUENT

Select either the function 1 prime ALL REAGENTS or move the cursor next to the required

reagent and press ENTER.

The priming cycle starts while the following menu is displayed :

ALL REAGENTS WAIT FOR 2 MN 3 S******************

IMPORTANT Before analyzing samples, visually inspect reagent lines and pumps for air bubbles.Repeat priming if air bubbles are still present. Call the ABX representative service department if priming does not eliminate air bubbles.

Never initiate two Lyse prime cycles back-to-back. This causes excessive foaming in thewaste chamber. Run a blank cycle between each Lyse prime cycle.


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IMPORTANT

From the REAGENT PACK menu, the function 2 "CBC LEFT" displays the number of analysis

cycles left to run with the same pack.

It is also possible to run a priming cycle at any time using the selection 3 "PRIME REAGENTS"

of the SERVICE menu.

Once the new PACK is installed a priming cycle will be automatically carried out and thefollowing menu is displayed.

PRIME WAIT FOR 2 MIN 3 S* * * * * * * * * *

Move the cursor to 3 PRIME REAGENTS and press the ENTER key. Select the function

1 CHANGE PACK and follow the instructions given by the LCD in order to install the pack.

REAGENT PACK > 1 CHANGE PACKHH : MM 2 CBC LEFT < 150>

Reagent pack



SERVICE > 2 DRAIN CHAMBER

HH : MM 3 PRIME REAGENTS

IMPORTANT Before analyzing samples, visually inspect reagent lines and pumps for air bubbles.

Repeat priming if air bubbles are still present. Call the ABX representative service department if priming does not eliminate air bubbles.

It is recommended not to remove the pack several times before the reagents aretotally used in order to avoid leak problems.



INSTALLATION

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Once the instrument ready for the analyses, remove the adhesive protection from the frontpanel (diag 31)

Diag.31



REQUIRED PRODUCTS

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RAS 167 A Ind.A

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


SAMPLING NEEDLE MAINTENANCE

18/06/98- Needle O ring replacement- Sampling needle replacement- Piercing needle replacement

- Hexagonal keys- Dynamometric screw driver A302 : MAG 019 A

- Silicone grease : LAM 004 A

- 15min

- See frequency chart table for cleaning.

- O ring replacement : 1/year- Needle replacement : On request only

- O ring kit : XEA 328 AS- Sampling needle : GBC 052 A- Piercing needle : GBC 189 A



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Page 2/3 RAS 167 A Ind.A

PROCEDURE

A - Needle or O ring replacement

- Disconnect the tube 1 from the

top of the needle 2 (diagram 1).

Remove the clip 3 (diagram 2) and

manually lift up the sampling needle(Diagram 1).

- Unscrew the 2 screws 3 (Diagram

1) in order to freed the needle rinsing

block 4 from the carriage frame

5 .

- Remove the rinsing block/needle

assy from the carriage taking care notto bend the needle.

- Lift up the O ring holder 1 and re-

place the O rings 2 by new ones

previously greased. Wipe all excess ofgrease away.- If necessary clean the inner surfaceof the rinsing block with a little piece ofpaper.

Proceed the same way to replace the needle if necessary

- Reassemble in the reverse order. Use a dynamometric screw driver to tighten the screws 3

(Diag.1) to 700 mN.m (99.4 Ozf.in).

Diag.1

Diag.2



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B - Piercing needle replacement

- Disconnect the tube 1 from the piercing needle 4 (Diag.3).

- Lift the needle 2 in the upper po-

sition.

- Loosen the 2 screws 3 just

enough to enable the rinsing blockto be lifted up of about 5 mm.

- Pull the piercing needle 4

(foreward) and replace it by a newone if necessary.- Replace the piercing needle O ring

5 (FAA 036 A) by a new one.

- Reassemble in the reverse order.Apply the same torque : 700mN.m(99.4 Ozf.in) to tighten the two

screws 3 .

Blockage problems may occur on someinstruments during the needle or carriagemotions giving some motor error messa-ges.

Before replacing the concerned motor, it isnecessary to check the correct rotation ofthe free puley located at the end of the

notched belt.

Remove the axle screw of the puley andclean its 2 parts and the washer. Reinstallthe puley assy, the rounded edge facingthe puley. Tighten the screw with a torqueof 400mN.m (56.8 Ozf.in).

NOTE It is recommended to check the correct motion of the needle.Proceed as follows :Enter the «SERVICE» menu and then the sub menu «MECHANIC»

and perform a «NEEDLE U/D» cycle.

Diag.3

Diag.4

Check that the puley turns freely after the tightening. Add a drop of oil (LAM 007 A) if necessary.

1 - FAG 011 A : Autolub. axle2 - GBC 146 A : Free pulley3 - GBC 147 A : Pulley holder



REQUIRED PRODUCTS

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REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY



10/04/98- Needle replacement- O ring replacement

- Hexagonal keys : 2,5- Dynamometric screw driver A300 : MAG 013 A

- Silicone grease : LAM 004 A

- 15 min

- Needle replacement : On request only- O ring replacements : 1/year

- O ring kit : XEA 328 AS- Spare parts kit : XEA 458 AS



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PROCEDURE

- Disconnect the tube 7 from the top of the needle. Manually lift up the sampling needle

(Diagram 1).

- Unscrew the 2 screws 5 in order to freed the needle rinsing block 2 from the carriage

frame. Remove the clip 6 .

- Remove the rinsing block/needle assy from the carriage taking care not to bend the needle.

- If necessary clean the inner surface of the rinsing block by means of a little piece of soft paper.

Spread a little amount of grease in between the rinsing block 2 and its support.

- Lift up the O ring holder 4 and replace the O rings 3 by new ones previously greased.

Wipe all excess of grease away.

Diag.1

NOTE Proceed the same way to replace the needle if necessary



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- Reassemble in the reverse order. Use a dynamometric screw driver to tighten the screws 5

(Diag.1) to 100 mN.m.

Blockage problems may occur on some instruments during the needle or carriage motionsgiving some motor error messages.

Before replacing the concerned motor, it is necessary to check the correct rotation of the freepulley located at the end of the notched belt.

Remove the axle screw of the pulley and clean its 2 parts and the washer.Reinstall the pulleyassy, the rounded edge facing the pulley. Tighten the screw with a torque of 400mN.m (99.4Ozf.in).

Check that the pulley turns freely after the tightening. Add a drop of oil (LAM 007 A) if necessary.

1 - FAG 011 A : Autolub. axle

2 - GBC 146 A : Free pulley

3 - GBC 147 A : Pulley holder

NOTE It is recommended to check the correct motion of the needle.Proceed as follows :Enter the «SERVICE» menu and then the sub menu «MECHANIC»and perform a «NEEDLE UP/DOWN» cycle.

Diag.2



REQUIRED PRODUCTS

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CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


CHAMBER MAINTENANCE

10/04/98- RBC & WBC/HGB cleaning- Aperture O ring replacement- Coaxial O ring replacement

- Hexagonal keys : 2,5- Soft paper- Dynamometric screw driver : MAG 013 A- Cutting pliers

- Liquid soap- Distilled water- A scalpel- A Micropipette tip

- 30 min

- RBC & WBC cleaning : 2 (type 1 & 2) or 3/year (type 3).- Aperture O ring replacement : 1/year- Electrode O ring replacement : 1/year

- O ring kit : XEA 328 AS



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PROCEDURE

1 - RBC chamber cleaning :

• Electrode O ring replacement : - Use a previously cutmicropipette tip to replace theelectrode O ring as shown onDiag.2.

• Aperture O ring replacement :

- Install the chamber over a piece

of white paper or cloth.- Carefully remove the counting

head 4 and plunge the aper-

ture 5 in distilled water.

Replace the O ring 2 by a new

one.

Diag.1

Diag.2

- Clean the chamber and the counting head with liquid soap, do not introduce any sharp instru-ments inside so as to avoid damaging the inside of the chamber and the aperture.

- Run a drain chamber cycle(SERVICE menu, DRAINCHAMBERS Sub menu).- Record the tube positionsbefore dismantling thechambers.- Disconnect the chamber tubes.- Unclip the RBC chamber.- Dismantle the electrode

6loosening the 2 fixation

screws 7 and the terminal

holding screw 3 (Diagram 1).



CHAMBER MAINTENANCE

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CAUTION

CAUTION Do not manipulate the aperture using hard instruments. Clean the aperturewith a piece of soft paper or preferably, in between 2 fingers.

- Do not apply too much pressure on the electrode fixation screws, as it canbreak the aperture (tightening torque = 100mN.m / 14.2 Ozf.in).- It is recommended to reconnect the tubes on the counting head beforereassembling the "electrode/chamber/counting head" assy in order to avoid

applying constraint on the chamber.

- Rinse thoroughly with distilled water- Dry the exterior of the chamber with a soft paper.

- Position the chamber in its fixation clips.- Reconnect the tubes

Diag.3

2 - WBC/HGB chamber cleaning :

- Run a drain chamber cycle(SERVICE menu, DRAINCHAMBERS Sub menu).

- Loosen the cover screws ofthe WBC/HGB chamber andremove the cover.- Record the tube positionsbefore dismantling thechambers.- Disconnect the chamber tu-bes.- Unclip the RBC chamber.- Dismantle the electrode

6 loosening the 2 fixation

screws 7 and the termi-

nal holding screw 3 .

- Proceed as described in 1 - RBC chamber to clean thechamber and to replace thelectrode and aperture Orings.

NOTE The spectrophotometer can not be dismantled from the chamber. If this one

has been dammaged it is necessary to replace the whole chamber assy.When cleaning the spectrophotometer, make sure to thoroughly rinse it inorder to obtain a correct HGB blank measure.



REQUIRED PRODUCTS

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CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


LIQUID VALVE MAINTENANCE

14/04/98Liquid valve assy replacementValve body replacement

Hexagonal keysPair of pliers

Soft paper

15 min

On request only

6 Valve assembly (MICROS 60 CT ) : XDA 579 CS6 Valve assembly (MICROS 60 OT ) : XDA 578 CS5 Valve assembly : XDA 580 CS2 ways NC liquid valve without solenoïd : XDA 481 B3 ways liquid valve without solenoïd : XDA 483 BSolenoïd 24V 4W : EAZ 004 A



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CAUTION

PROCEDURE

- Several reasons can unable the correct operations of the valves :

- Leaks on the valve bodies- Defective Solenoïds- Liquid discharge on valves

- Corrosion traces on the axis, ect...

- Run a «DRAIN CHAMBERS» cycle.

- Switch off the instrument.

- Note the tube positions on the valve assembly that requires to be dismantled.

- Unscrew the valve assembly fixation screws (Diag.1).

- If only one valve has been damaged it is possible to dismantle the valve body on its own asshown on the diagram 3 : use a pair of pliers to disconnect the valve holder clip and remove thebody.

NOTE When leaks occur on valves it is recommended to replace theentire valve assembly by a new one.

Diag.1 Diag.2

- Disconnect the supplying flat cable from the valve assembly (diag.2).

When replacing one valve only, it is important to check if the «neighboured»valves have not been damaged too.



LIQUID VALVE MAINTENANCE

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When replacing one valve only, it is important to check if the «neighboured» valves have notbeen damaged too.

- Reconnect the tubes on the nipples (with the sleeves).- Re-install in the reverse order. Switch on the instrument.- Control the watertightness of the valves and check for the correct operations :Go to «SERVICE» menu, then to «MECHANIC» sub menu (5) and require a «VALVES» (6) test(see Mechanic functions : RAS 173 A).- Check the calibration too.

• REMARKS

On MICROS 60 - CT , when piercing several times the same tube cap, some pieces of cork may

be dragged along towards the WBC/HGB chamber and then the liquid valve .

This may damaged the operation of the valve.

CAUTION The solenoïd can not be dismantled unless unsoldering it.If this one is suspected solder a new one or replace the entire valve assembly.

Diag.3



REQUIRED PRODUCTS

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CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


POWER SUPPLY CHECK OR REPLACEMENT

10/04/98- Voltage supply check- Power supply module replacement- Fan operation check

- Hexagonal keys- Flat screw driver- Volmeter

- None

- 15 min

- 1/year

- None



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CAUTION

PROCEDURE

1 - Supply voltage check

- The supply voltage check is done on the mother board (XAA 355 A) according to the followingchart table :

* Ground on TP31, TP 30 or TP 29

CAUTION The supply voltage check has to be done with the power supply module connectedonly.

TEST POINTS VOLTAGES

TP 20 -12V + 0,5V

TP 22 24V + 1.5V - 0V

TP 23 5V + 0,3V - 0V

TP21 12V + 0,5V

The two screws on the top of the coaxial cover holding the board on theMICROS frame are the grounding connection of the mother board (diag 1).It is mandatory to check the correct tightening of these screws to obtaincorrect voltage values.

Diag.1



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POWER SUPPLY CHECK OR REPLACEMENT

The test points are located as shown on the diagram below :

NOTE

If the voltages values are not correct or among the ranges no adjustmentcan be carried out either on the board or on the power supply module.

Replace the power supply module as described below :

Diag.2



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2 - Power supply module replacement- Switch off the instrument.- Disconnect the main supply voltage cable and the printer cable.- Disconnect the power supply cable from the mother board, connector J26 (see Diag.2).

- Unscrew the 4 screws (Diag.3) and start to move out the power supply module. Disconnect

the grounding wire (Yellow/green wire see diag 4) from the rear panel of the module.Route the cable (from J26) down to make the removing of the module easier.

WARNING ! The power supply module internal fuse is not to be replaced even when thisone has blown down.

- Replace the power supply module by a new one and reinstall in the reverse order.- Switch on the instrument and check the voltages on the mother board as described in theprevious paragraph.

• REMARKSThe four leds in front of the test points are lit to indicate a voltage presence but whatever itsvalue!!!

Check the operation of the fan as following : When the fan has stopped, move the cursor to the4 SERVICE menu and press ENTER : the fan should start.

Diag.3 Diag.4



REQUIRED PRODUCTS

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CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


TECHNICIAN FUNCTION

16/06/98

1 - Version display.

2 - AdjustmentsHGB photometer calibration, Aperture voltageVacuum check, WBC gain, RBC & PLT gainSensor, Needle heigth, Needle motionBubbling

- Flat screw driver- thermometer- Barflex- Hexagonal keys

- Voltmeter

- WBC latex : LAD 001 AS- RBC and PLT latex : LAD 002 AS- Soft paper- Flat piece of stiff plastic

- 60 min

- See maintenance chart table.

- Needle position tool : GBC 218 A

3 - Temperature sensor adjustment

4 - Run mode5 - Reagent pack6 - Serial number7 - Cycle number8 - Burn-in

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PROCEDURE

7 - TECHNICIAN

1 - VERSION

2 - ADJ UST MENT S

3 - T EMPERAT URE

4 - RUN MO DE

5 - REAG ENT PACK

6 - SERIAL #

7 - CYCLE #

8 - BURN- IN

1 - CAL. PHOTOMETER

2 - APERT URE VO LT AG E

3 - CHECK PRESSURE

4 - WBC ADJ UST

5 - RBC PLT ADJUST

6 - SENSO R ADJ UST

7 - POSIT ION ADJUST

1 - NEEDLE SENSO R

1 - NEEDLE

2 - CARRIAGE

1 - UNCAL. T EMPERAT URE

2 - CALIBRATE

3 - T EMPERAT URE

1 - VISIBLE

2 - INVISIBLE

1 - CONTROL

2 - USER

1 - YES

2 - NO

1 - STARTUP # < >

2 - STANDBY # < >

3 - CBC # < >

1 - BURNIN #

2 - BURNIN

3 - CONTROL

MICROS 60 OT

1 - RBC PLT ADJUST

2 - MCV < >

3 - MPV < >

1 - WBC ADJ UST

2 - # LYM < >

3 - # GRA < >

1 - NEEDLE < >

2 - ADJ UST MENT

3 - CHECK

1 - CARRIAGE < >

2 - ADJ UST MENT

3 - CHECK

8 - BUBBLING ADJUST 1 - BUBBLING 1 < >

2 - BUBBLING 2 < >

3 - CHECK



TECHNICIAN FUNCTION M I C

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Once entering the «SERVICE» sub menu, move to «TECHNICIAN FUNCTIONS» and press

. A specific password (421) is required to enter the sub menus.

Move the cursor by means of and and choose the required menus pressing

the key.

I - The version number is displayed.

II - Adjustments :

1 - HGB photometer calibration

- Dismantle the WBC/HGB chamber cover.

- Check the general cleanliness of the WBC chamber/spectrophotometer assy.

- Re-install the chamber cover.

NOTE If the WBC chamber has been dismantled previously make sure no liquidhas flown in between the spectrophotomer and the chamber.Clean the inner surfaces of the spectrophotometer as well as the chamber.Reassemble the assy and tighten the two screws to the following torque :400mN.m (see RAS 169 A : Chamber maintenance)

- Run the CAL PHOTOMETER function (function 1 of the «ADJUSTMENTS» menu) : diluent

is delivered to the WBC/HGB chamber twice.

An HGB channel is displayed on the LCD screen :

VALUEXXX

IMPORTANT 1 - The HGB photometer calibration must be done 20min at least after the instrumenthas been switched on.2 - This adjustment must be done with the WBC chamber cover installed!!!

- By means of R97 (See diagram 1) adjust the HGB channel according to the room temperatureusing the chart table given on next page.

NOTE After 40 seconds approximately, the function is automatically exited.

- Run the CAL PHOTOMETER function again to verify the adjustment.



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Diag.1

R O O M

T P T

(°C )

C H A N N E L

M in i. N o m in al M a xi.

1 5 2 40 2 4 5 2 50

1 6 2 40 2 4 5 2 50

1 7 2 39 2 4 4 2 49

1 8 2 38 2 4 3 2 48

1 9 2 37 2 4 2 2 47

2 0 2 36 2 4 1 2 46

2 1 2 35 2 4 0 2 45

2 2 2 34 2 3 9 2 44

2 3 2 34 2 3 9 2 44

2 4 2 33 2 3 8 2 43

2 5 2 32 2 3 7 2 42

2 6 2 31 2 3 6 2 41

2 7 2 30 2 3 5 2 40

2 8 2 29 2 3 4 2 39

2 9 2 28 2 3 3 2 38

3 0 2 28 2 3 3 2 38

3 1 2 27 2 3 2 2 37

3 2 2 26 2 3 1 2 36

3 3 2 25 2 3 0 2 35

3 4 2 24 2 2 9 2 34

3 5 2 23 2 2 8 2 33

R O

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2 - Aperture voltage

- Once entering the «APERTURE VOLTAGE» menu, connect a voltmeter between the ground(TP30) and TP19.

- Check that the value is 60V +2.8V -1.5V (Diagram 1). The aperture voltage is not adjustable.

- Press any key to escape.

3 - Vacuum check

Enter the 2 - ADJUSTMENTS / 3 - CHECK PRESSURE menu.

- Disconnect the tube from the vacuum/waste

syringe coming from the valve (see

diagram 2).

- Follow the instructions given on the LCDscreen :

«PLEASE PLUG BARFLEX ON AIRSYRINGE» (On the free nipple).

«CHECK PRESSURE : -200mB + 10mB»(The piston has raised in order to create avacuum in the syringe body).

- Check the stability of the vacuum during30 secondes : The vacuum drop down mustbe < 2 mbar.

Diag.2

- If the results are not correct check the O ring and the tubing watertightness.

«PLEASE RECONNECT PREVIOUS TUBE» (disconnect the Barflex and replug the tubeinstead).

4 - WBC adjust

- Put the WBC latex to mix on a Vortex during 1min or shake thoroughly

- Run a blank cycle to check the cleanliness of the instrument.

- Enter the 4 - WBC ADJUST sub menu and then 1 - WBC ADJUST

IMPORTANT As the WBC gain is a factory adjustment it is mandatory not to readjust it without takingthe following precautions :Carry out previously, an autoconcentrated cleaning to make sure of the cleanliness ofthe WBC counting circuit.If necessary clean the WBC chamber aperture as described in RAS 169 A.Make sure the Latex has been thoroughly mixed before.

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- Present the vial of Latex to the open probe as shown ondiagram 3 and press the sampling bar located behind thesampling needle : an analysis cycle begins.

During the cycle measuring phasis (around 1 minute) theLymphocyte and the Granulocyte volumes are displayedon the screen every 3 seconds as shown below :

- Wait for several results to be displayed and check the stability of both values.

After one minute the last volume values displayed on the screen are printed out as well as the WBC,PLT and RBC histograms and the CBC's results. Check that these printed values correspond to thefollowing target values :

- LYM = 57 + 1- GRA = 180 + 2

- If not rerun a «WBC ADJUST» cycle after having previously mixed the latex vial again. By meansof R74 (see diagram 4) adjust the volumes to the target values during the measuring phasis.

LYM <57 +/- 1> GRA <180 +/- 2>57 180

IMPORTANT It is mandatory not to operate the gain adjustment as long as the lympho and granulovalues are not stable.

Diag.4

Diag.3

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5 - RBC PLT adjust

- Put the RBC and PLT latex to mix on a Vortex during 1min or shake thoroughly


- Enter the «5 - RBC PLT ADJUST» sub menu.

- Present the vial of Latex to the open probe as shown on diagram 3 and press the samplingbar located behind the sampling needle : the needle directly delivers the latex sample in theRBC chamber dilution and a measuring phasis begins.

- During the cycle measuring phasis (around 1 minute) the Platelet and the Red Blood cellvolumes are displayed on the screen every 3 seconds as shown below :


IMPORTANT As the RBC/PLT gain is a factory adjustment it is mandatory not to readjust it withouttaking the following precautions :Carry out previously an autoconcentrated cleaning to make sure of the cleanliness ofthe RBC/PLT counting circuit.If necessary clean the RBC/PLT chamber aperture as described in the procedure RAS169 A.Make sure the Latex has been thoroughly mixed before.

RBC <74 +/- 1> PLT <59 +/-1>74 59

IMPORTANT It is mandatory not to operate the gain adjustment as long as the platelet and RBCvalues are not stable.

NOTE Both sub menus 2 - # LYM < > and 3 - # GRA < > allow thetechnician to change the Latex target values if the latex run onthe instrument different from the Latex recommended above.

After one minute the last volume values displayed on the screen are printed out as well as thePLT and RBC histograms and the CBC's results.

- Check that these printed values correspond to the following target values :

- RBC = 74 + 1- PLT = 59 + 1

NOTE From the latex lot # 980311 included, balls having a different size, a drift ofMPV peak has been noticed, i.e. a modification of the PLT gain target value: it becomes 64 instead of 59.

The program default value will be modified in the next MICROS version.Both sub menus 2 - MCV < > and 3 - MPV < > allow the technician to changethe Latex target values. If the lot # > 980311, modify the target values andproceed the same way to adjust the PLT gain.



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- If not, rerun a «RBC PLT ADJUST» cycle after having previously mixed the latex vial again.

- Adjust the volumes to the target values during the measuring phasis by means of the followingpotentiometers (see Diagram 4) :

- RBC gain with R81

- PLT gain with R87

6 - Needle sensor adjust

If the needle detector has been replaced by a new one or dismantled for any reason, it ismandatory to re-position it at the right heigth. Proceed as following :

- Install the piece of plastic (diagram 5)underneath the needle rinsing block.

- Once entering the menu «6 - SENSORADJUST», enter the sub menu «1 - NEEDLE

SENSOR».- Push the sampling needle downward until itstops against the piece of plastic and press anykey in order to raise the needle back in its upperposition.

- For a current number of steps out of ranges,unloosen the 2 cell fixation screws (diagram 6)and gently move the sensor

- upward if the current value is too low- downward if the current value is too high.

- Tighten the screws and rerun a «NEEDLESENSOR» cycle. Check that the current valueis correct.

CURRENT : 62 MIN : 65 MAX : 75

NOTE 10 steps correspond to around 1 mm. The target number of steps is 70 + 5

Diag.5

The current number of steps, the mini and maxivalues are displayed as well as the way to movethe sensor (shown by an arrow) if the currentvalue is out of ranges (see below).

Diag.6

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Diag.7

7 - Position adjustment

• Needle heigth adjustmentWhen replacing a needle, it is mandatory to adjust the heigth of the needle in the chambers.Proceed as follows :- Remove the WBC/HGB chamber cover.

- Enter the menu 7 - POSITION ADJUST / 1 - NEEDLE / 2 - ADJUSTMENT.- Manually pull down the needle until it comes into contact with the edge of the RBC chamber(Diag. 7).- Press a key to continue : the needle moves up to the initial position ; the value is stored.

• Needle motion adjustmentThe needle position in the WBC chamber can be adjusted as follows :

- Enter the menu 7 - POSITION ADJUST / 2 - CARRIAGE / 2 - ADJUSTMENT.- Position the tool (P/N : GBC 218 A) over the RBC and WBC chambers (Diag 8).- Manually lower the needle into the WBC chamber.- Press a key to continue : the needle moves up, and the carriage comes back to the initialposition ; the value is stored.- Carry out a 3 - CHECK cycle : the needle comes down to the WBC chamber.- Check that the needle is centered in the hole.

If not, enter the 2 - CARRIAGE / 1 - CARRIAGE < > menu ; the display shows the currentnumber of steps carriage motion.If the needle goes too far on the right, add 1 step to the current value for 0.1mm.If the needle is too much on the left, decrease the current value of 1 step for 0.1mm.

CARRIAGE ? : EXIT : ESCCURRENT : 893 SAVE : ENTER

Carry out a 2 - CARRIAGE / 3 - CHECK cycle again to control the needle position.

Diag.8

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8 - Bubbling adjustmentAn overflow protection tank is installed on the drain circuit of each chamber. This one preventsfrom polluted liquid overflow during bubbling phasis (Diag .9).

CAUTION These values are factory adjusted (and may be different from the default valuesshown above) and should be modified only when hematologic erroneous resultsare given by the instument : If values are too important, liquid overflows canoccur or if bubbling is too low homogeneity of the dilution can be decreased.Ranges : 150 < BUBBLING 1 < 200

80 < BUBBLING 2 < 140

To modify the bubbling values, enter the menu :2 - ADJUSTMENTS / 8 - BUBBLING ADJUST. / BUBBLING 1 < >2 - ADJUSTMENTS / 8 - BUBBLING ADJUST. / BUBBLING 2 < >

and type in new step value.

Carry out a 3 - CHECK to control the adjustment.

Two bubbling phasis are adjustable :- "BUBBLING 1" is the first dilution (WBC/HGB chamber) bubbling value.- "BUBBLING 2" is the second dilution (WBC/HGB chamber + lyse) value and RBCchamber bubbling value.

Both values correspond to a number of steps carried out by the waste/vacuum syringe.Default values are BUBBLING 1 : 175

BUBBLING 2 : 120

Diag.9




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III - Temperature

When entering the «temperature adjustment» menu the following sub menus are displayed :

1 - Uncal. temperature

When pressing the key the sensor temperature value uncalibrated is displayed. This

value should be close to the diluent temperature.

2 - Calibrate :The temperature must be calibrated according to the diluent temperature :

- Plunge a thermometer directly into the diluent container and leave it for a while until stabilization.

IMPORTANT The startup, stand by and CBC numbers are adjustable in this menu but it ismandatory to keep the initial values (useful for maintenance schedules).

VIII - Burn-in

This function which allows the burn-in of the instrument is intended for a factory use.

- Run 2 diluent primes («SERVICE» menu, «PRIME» sub menu, «DILUENT» selection).

- Enter the «CALIBRATE» menu. Note the temperature of the diluent and type in the value (ifit is different from the previous on the instrument).

- Press to save the new value.

3 - Temperature :

1 - Visible : Press to validate the temperature display.

The value displayed is the calibrated temperature.

2 - Invisible : Press to cancel the temperature display.

(Temperature invisible by default)IV - Run mode

The instrument must be configurated in the «USER» mode (configurated by default in the«USER « mode), the «CONTROL» mode intends for a factory use.

V - Reagent pack

This function is used to update the instrument from a bottle mode to a pack mode.

VI - Serial #

Displays the instrument serial number.

VII - Cycle #

Displays the : Startup number since the first use of the instrument.Stand by number since the first use of the instrument.CBC number since the first use of the instrument.

NOTE For a pack equipped instrument, the thermometer must be plunged in theWBC/HGB chamber and the temperature must be note as soon as possible.



REQUIRED PRODUCTS

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RAS 173 A Ind.A

• CONCERNS

• REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


MECHANIC FUNCTION

08/04/98- Sensor replacements- Needle motion check- Carriage motion check- Liquid syringe motion check

- Vacuum/waste syringe motion check- Valve operation check- LCD contrast adjustment- Piercing mechanism check (MICROS 60 CT )

- Hexagonal keys- Felt-pen

- None


- Vacuum/waste syringe sensor : XBA 319 AS- Liquid syringe sensor : XBA 319 AS- Carriage and needle sensors : XBA 250 A

- 30 minutes



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CAUTION

PROCEDURE

The MECHANIC function arborescence is as follows :

5 - M ECHANIC 1 - Sensors

2 - Needle U/D

3 - Carr iage L/R

4 - L iquid syr inge

5 - Pres. syr inge

6 - Valves

7 - Chg. Contrast

8 - Park

9 - Piercing

From the main menu, enter the 4 - SERVICE menu and move to 5 - MECHANIC sub menu.

1 - Sensors

Press ;

NEEDLE SENSOR 0CARRIAGE SENSOR 0

should be displayed on the LCD screen.

Manually raise the needle support in the upper position (see diag 1)The «0» should switch to «1». This indicates the correct operation of the needle sensor (diagram1).If nothing happened try to move the needle up and down again to get the commutation from«0» to «1».If the test is still wrong, check the correct connection of the sensor on the connector J7 of themother board.

• Replacing the needle sensorSwitch off the instrument.Use a felt-pen to mark the sensor position.Unscrew the 2 sensor fixation screws and remove the cell holder .Disconnect the wire from the J7 connector (See diagram 2).Replace the previous sensor by a new one and reassemble in the reverse order.

The new sensor must be installed exactly on the same position.

Proceed as described in RAS 172 A (MICROS 60OT ) or RAS 176 A (MICROS 60CT ) «Sensoradjustment» to check the correct position of the sensor.



MECHANIC FUNCTION

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CAUTION

Diag.1

Diag.2

• Replacing the carriage sensorUse the 1 - SENSOR test to check the carriage motion detection :Move rightward the needle carriage in order to perform the sensor detection (diagram 3).To replace it, note its position with a felt-pen, unscrew the cell fixation screws and remove the

cell holder.Disconnect the wire from the connector J8 (See diagram 2)Replace the previous sensor by a new one and reassemble in the reverse order.

The new sensor must be installed exactly on the same position.

Proceed as described in RAS 172 A (MICROS 60 OT ) or RAS 176 A (MICROS 60 CT ) «Sensoradjustment» to check the correct position of the sensor.

MICROS 60 OT MCROS 60 CT



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2 - Liquid syringes motion checkRun a 4 - LIQUID SYRINGE cycle : The syringes (diag 4) are moved upward and downward

once.If the sensor (diag 5) detection is not correct a message «ERROR : SYRINGE MOTOR» isdisplayed.

Three parts are involved :

- The motor ———> Check the connection on the board (connector J3)- The syringes ———> Check the motion up/down- The sensor ———> Check the connection on the board or replace it.

SYRINGE FRONT SIDE VIEW SYRINGE BACK SIDE VIEW

Diag.3

Diag.4 Diag.5

• Replacing the sensor :

- Switch off the instrument.- Unscrew the 2 screws (Diag.5) and disconnect the wire from the connector J9 (Diag.2)

- Replace the previous sensor by a new one and re-install in the reverse order.- Switch on the instrument.- Run a 4 - LIQUID SYRINGE cycle again to control the correct detection.

MICROS 60 OT MICROS 60 CT



MECHANIC FUNCTION

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3 - Vacuum/waste syringe motion check

Run a 5 - PRESSURE SYRINGE cycle : The syringe piston moves upward and downwardonce.If the sensor (Diag.6) detection is not correct a message «ERROR : PRESSURE SYRINGE

MOTOR» is displayed.

Three parts are involved :

- The motor ———> Check the connection on the board (connector J4)- The syringe ———> Check the motion up/down- The sensor ———> Check the connection on the board or replace it.

Diag.6

• Replacing the sensor :

- Switch off the instrument.

- Unscrew the 2 cell fixation screws (Diag.6) and disconnect the wire from the connector J10(Diag.2)- Replace the previous sensor by a new one and re-install in the reverse order.

- Switch on the instrument.- Run a 5 - PRESSURE SYRINGE cycle again to control the correct detection.

4 - Valve operation check

Run a 6 - VALVES cycle. The number of the valves from 1 to 13 is displayed and each valveis activated once.Check their correct operations. If a valve is suspected proceed as described in the procedure: RAS 170 A.



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5 - LCD contrast adjustment

Enter the sub menu «7 - CHG. CONTRAST». Use the arrows to modify the contrast and press

to validate the adjustment.

6 - Piercing mechanism check (MICROS 60 CT)

Enter the sub menu 9 - PIERCING. Follow the instructions : «PLEASE CLOSE TUBE HOLDERDOOR» : a piercing operation is simulated.

This function gives the sample tube holder position and the heigth of the needle in its lowerposition. See RAS 172 A (MICROS 60 OT ) or RAS 176 A (MICROS 60 CT )

NOTE If the LCD screen happens to be unreadable because of a bad contrastadjustment it is possible to get back a correct contrast pressing at the

same time both keys :+

.

NOTE If the sample tube holder has been removed the following message isdisplayed : «ERROR : NO SAMPLE TUBE HOLDER».If the sample tube holder has been turned in between two piercing posi-tions (it means that the tube is not in front of the piercing needle) thefollowing message is displayed : «ERROR : TUBE HOLDER POSITION».



REQUIRED PRODUCTS

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RAS 174 A Ind.B

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


DRAIN DETECTION

05/10/98- Drain detection sensor adjustment/replacement

- Voltmeter- Flat screw driver- 5ml syringe

- None

- 15 min

- On request or once a year

- Drain detection sensor : XBA 199 A.

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PROCEDURE

- Make sure the cell is perfectlydrained (no bubble).

- Connect a voltmeter betweenthe ground TP30 and TP6 (SeeDiag.2) and adjust the voltage to4,5V + 0,3V by means of R6.

Diag.1

The Vacuum/Waste syringe and the chambers drains are controled by an infrared sensor locatedbelow the syringe.

Controls carried out by the cell during a cycle are as follows :

- Control of the correct operation of the cell

At the first chamber drain, air must be detected in the cell within defined timeout, and followedby liquids.If this switch "air-liquid" has been successfull, the adjustment of the cell (see below) is validated.If not, the cycle is stopped and the following message is triggered : "sensor error or diluent empty " (check the connection or the adjustment of the cell).

- Control of the chamber drains

A first measure controls that liquids circulate in the cell during a defined timeout.The second measure checks that air has replaced liquid.If so, the drain phasis is validated.

If not, the following message is displayed : «.....» (the instrument carries on the current cycle)It means that the sensor always detects liquids.(Check the watertightness of the syringe.)

• Drain detection sensor adjustment

- Raise the piston up and press the valve <2>.- Manually perform a syringe drain pressing the valve <5> and pulling down the syringe piston(Diag.1).



DRAIN DETECTION

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- Push diluent through the sensor and check that the voltage falls down below 1Volt on thevoltmeter.

- If nothing happens switch off the instrument and disconnect the connector from J13.- Replace the drain detection sensor by a new one.- Switch on the instrument- Carry out the new sensor adjustment as described above.

NOTE Check the commutation from 4,5Volts to ~ 0Volt once againpushing and drawning alternately liquid and air through the sensorby means of the syringe.

MICROS 60 MOTHER BOARD

Diag.2

Diag.3

Fill the syringe up with diluent and connect it on the detection cell as shown on diagram 3.

NOTE However it is posible to control the correct operation of the sensorby means of the LED located next to R6.Indeed the LED should be lit when liquid circulates through thesensor and switched off with air.



REQUIRED PRODUCTS

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RAS 175 A Ind.B

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


PCB VOLTAGE CHECKS/ ADJUSTMENTS

01/10/98- Voltage supply check- Aperture voltage check- RBC threshold check/adjustment- WBC threshold check/adjustment- PLT threshold check/adjustment

- HGB blank voltage check.- Stepper motor voltages adjustment

- Voltmeter- Flat screw driver

- None

- 20min


- None



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PROCEDURE

1 - RBC, PLT, WBC threshold checks/adjustments

- Ground on TP31.

- Adjust the thresholds according to the below chart table :

(See Diag.1)

2 - Aperture voltage checkSee RAS 172 A : MICROS 60 OT Technician function

RAS 176 A : MICROS 60 CT Technician function

3 - Voltage supply checkSee RAS 171 A

4 - HGB blank voltage checkSee RAS 172 A : MICROS 60 OT Technician function

RAS 176 A : MICROS 60 CT Technician function

5 - Stepper motor voltage checks/adjustments- Ground on TP31.- Adjust the motor voltages according to the below chart table :

THRESHOLDS TEST POINTS VOLTAGE POTENTIOMETERS

WBC TP 10 350 mV + 7 R68

RBC TP13 400 mV + 7 R75

PLT TP16 180 mV + 3 R82

MOTORS TEST POINTS VOLTAGES POTENTIOMETERS

Air syringe TP36 2.50 V + 0.05 V R195

Liquid syringe TP35 2.00 V + 0.05 V R186

Horizontal carriage TP37 1.50 V + 0.05 V R177

Vertical carriage TP38 1.00 V + 0.05 V R168

(See Diag.1)



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PCB VOLTAGE CHECKS/ADJUSTMENTS

Diag.1



REQUIRED PRODUCTS

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RAS 176 A Ind.A

• CONCERNS

• REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


TECHNICIAN FUNCTION

16/06/98

1 - Version display.

2 - Adjustments :HGB photometer calibration, Aperture voltageVacuum check, WBC gain, RBC & PLT gainSensor, Needle heigth, Needle motionBubbling

- Flat screw driver- thermometer- Barflex- Hexagonal keys- Voltmeter

- WBC latex : LAD 001 AS- RBC and PLT latex : LAD 002 AS- Soft paper- Flat piece of stiff plastic

- 60 min


- None

3 - Temperature sensor adjustment

4 - Run mode5 - Reagent pack6 - Serial number7 - Cycle number8 - Burn-in

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PROCEDURE

7 - TECHNICIAN

1 - VERSION

2 - ADJUSTMENTS

3 - TEMPERATURE

4 - RUN MODE

5 - REAGENT PACK

6 - SERIAL #

7 - CYCLE #

8 - BURN-IN

1 - CAL. PHOTOMETER

2 - APERTURE VOLTAGE

3 - CHECK PRESSURE

4 - WBC ADJUST

5 - RBC PLT ADJUST

6 - SENSOR ADJUST

7 - POSITION ADJUST

1 - NEEDLE SENSOR

1 - NEEDLE

2 - CARRIAGE

1 - UNCAL. TEMPERATURE

2 - CALIBRATE

3 - TEMPERATURE

1 - VISIBLE

2 - INVISIBLE

1 - CONTROL

2 - USER

1 - YES

2 - NO

1 - STARTUP # < >

2 - STANDBY # < >

3 - CBC # < >

1 - BURNIN #

2 - BURNIN

3 - CONTROL

MICROS 60 CT

9 - SAMPLING POSITION

1 - NEEDLE ADJUSTMENT

2 - NEEDLE 1 < >

3 - NEEDLE 2 < >

4 - NEEDLE 3 < >

5 - NEEDLE 4 < >

6 - NEEDLE 5 < >

7 - NEEDLE 6 < >

8 - CHECK

1 - RBC PLT ADJUST

2 - MCV < >

3 - MPV < >

1 - WBC ADJUST

2 - # LYM < >

3 - # GRA < >

1 - NEEDLE < >

2 - ADJUSTMENT

2 - CARRIAGE SENSOR

3 - CHECK

1 - CARRIAGE < >

2 - ADJUSTMENT

3 - CHECK

8 - BUBBLING ADJUST 1 - BUBBLING 1 < >

2 - BUBBLING 2 < >

3 - CHECK



TECHNICIAN FUNCTION

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C T Page 3/14RAS 176 A Ind.A

Once entering the «SERVICE» sub menu, move to «TECHNICIAN FUNCTIONS» and press

. A specific password (421) is required to enter the sub menus.

Move the cursor by means of and and choose the required menus pressing

the key.

I - The version number is displayed.

II - Adjustments :

1 - HGB photometer calibration

- Dismantle the WBC/HGB chamber cover.

- Check the general cleanliness of the WBC chamber/spectrophotometer assy.

- Re-install the chamber cover.

NOTE If the WBC/HGB chamber has been dismantled previously make sure noliquid has flown in between the spectrophotomer and the chamber.Clean the inner surfaces of the spectrophotometer as well as the chamber.Reassemble the assy and tighten the two screws to the following torque :400mN.m (see RAS 169 A : Chamber maintenance)

- Run the CAL PHOTOMETER function (selection 1 of the «ADJUSTMENTS» menu) : diluent

is delivered to the WBC/HGB chamber twice.

An HGB channel is displayed on the LCD screen :

VALUEXXX

IMPORTANT 1 - The HGB photometer calibration must be done 20min at least after the instrumenthas been switched on.2 - This adjustment must be done with the WBC chamber cover installed!!!

- By means of R97 (See diagram 1) adjust the HGB channel according to the room temperatureusing the chart table given on next page.

NOTE After 40 seconds approximately, the function is automatically exited.

- Run the CAL PHOTOMETER function again to verify the adjustment.



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Diag.1

ROOM

TPT

(°C)

CHANNEL

Mini. Nominal Maxi.

15 240 245 250

16 240 245 250

17 239 244 249

18 238 243 248

19 237 242 247

20 236 241 246

21 235 240 245

22 234 239 244

23 234 239 244

24 233 238 243

25 232 237 242

26 231 236 241

27 230 235 240

28 229 234 239

29 228 233 238

30 228 233 238

31 227 232 237

32 226 231 236

33 225 230 235

34 224 229 234

35 223 228 233

TECHNICIAN FUNCTION

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2 - Aperture voltage

- Once entering the «APERTURE VOLTAGE» menu, connect a voltmeter between the ground(TP30) and TP19.

- Check that the value is 60V +2.8V -1.5V (Diagram 1). The aperture voltage is not adjustable.

- Press any key to escape.

3 - Vacuum check

- Disconnect the tube from the vacuum/

waste syringe coming from the valve

(see diagram 2).

- Follow the instructions given on the LCD

screen :

«PLEASE PLUG BARFLEX ON AIRSYRINGE» (On the free nipple).

«CHECK PRESSURE : -200mB + 10mB»(The piston has raised in order to create avacuum in the syringe body).

- Check the stability of the vacuum during30 secondes : The vacuum drop down mustbe < 2 mbar.

Diag.2

- If the results are not correct check the O ring and the tubing watertightness.

«PLEASE RECONNECT PREVIOUS TUBE» (disconnect the Barflex and replug the tubeinstead).

4 - WBC adjust

- Put the WBC latex to mix on a Vortex during 1min or shake thoroughly


- Enter the «WBC ADJUST» sub menu.

IMPORTANT As the WBC gain is a factory adjustment it is mandatory not to readjust it without takingthe following precautions :Carry out previously an autoconcentrated cleaning to make sure of the cleanliness ofthe WBC counting circuit.If necessary clean the WBC chamber aperture as described in RAS 169 A.Make sure the Latex has been thoroughly mixed before.

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After one minute the last volume values displayed on the screen are printed out as well as the WBC,PLT and RBC histograms and the CBC's results. Check that these printed values correspond to thefollowing target values :

- LYM = 57 + 1- GRA = 180 + 2

- If not rerun a «WBC ADJUST» cycle after having previously mixed the latex vial again. By means

of R74 (see diagram 4) adjust the volumes to the target values during the measuring phasis.

LYM <57 +/- 1> GRA <180 +/- 2>57 180

IMPORTANT It is mandatory not to operate the gain adjustment as long as the lympho and granulovalues are not stable.

Diag.4

- Enter the «WBC ADJUST» sub menu and close thedoor of the piercing mechanism (See Diag 3) : A CBC’scycle starts.

During the cycle measuring phasis (around 1 minute)the Lymphocyte and the Granulocyte volumes aredisplayed on the screen every 3 seconds as shownbelow :

Diag.3

TECHNICIAN FUNCTION

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5 - RBC PLT adjust

- Put the RBC and PLT latex to mix on a Vortex during 1min or shake thoroughly


- Enter the «RBC PLT ADJUST» sub menu.

- Present the vial of Latex to the open probe as shown on diagram 3 and press the samplingbar located behind the sampling needle : the needle directly delivers the latex sample in theRBC chamber dilution (for a usual analysis cycle the sample is first delivers to the mixing

chamber) and a measuring phasis begins.

- During the cycle measuring phasis (around 1 minute) the Platelet and the Red Blood cellvolumes are displayed on the screen every 3 seconds as shown below :


IMPORTANT As the RBC/PLT gain is a factory adjustment it is mandatory not to readjust it withouttaking the following precautions :Carry out previously an autoconcentrated cleaning to make sure of the cleanliness ofthe RBC/PLT counting circuit.

If necessary clean the RBC/PLT chamber aperture as described in the procedure RAS169 A.Make sure the Latex has been thoroughly mixed before.

RBC <74 +/- 1> PLT <59 +/-1>74 59

IMPORTANT It is mandatory not to operate the gain adjustment as long as the platelet and RBCvalues are not stable.

NOTE Both sub menus 2 - # LYM < > and 3 - # GRA < > allow thetechnician to change the Latex target values if the latex run onthe instrument is different from the one recommended above.

NOTE From the latex lot # 980311 included, balls having a different size, a drift ofMPV peak has been noticed, i.e. a modification of the PLT gain target value: it becomes 64 instead of 59.The program default value will be modified in the next MICROS version.

Both sub menus 2 - MCV < > and 3 - MPV < > allow the technician to changethe Latex target values. If the lot # > 980311, modify the target values andproceed the same way to adjust the PLT gain.



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- If not, rerun a «RBC PLT ADJUST» cycle after having previously mixed the latex vial again.

- Adjust the volumes to the target values during the measuring phasis by means of the followingpotentiometers (see Diagram 4) :

- RBC gain with R81

- PLT gain with R87

6 - Sensor adjust

• Needle sensorIf the needle sensor (diag 6) has been replaced by a new one or dismantled for any reason, itis mandatory to re-position it at the right heigth. Proceed as following :

- Install the piece of plastic (diagram 5)underneath the needle rinsing block.

- Once entering the menu «SENSOR ADJUST»,

enter the sub menu «NEEDLE SENSOR».- Push the sampling needle downward until itstops against the piece of plastic and press anykey in order to raise the needle back in its upperposition.

The current number of steps, the mini and maxivalues are displayed as well as the way toremove the sensor (shown by an arrow) if thecurrent value is out of ranges (see below).

- For a current number of steps out of ranges,unloosen the 2 cell fixation screws (diagram 6)and gently move the sensor

- upward if the current value is too low- downward if the current value is too high.

- Tighten the screws and rerun a «NEEDLESENSOR» cycle. Check that the current valueis correct.

CURRENT : XXX MIN : 422 MAX : 432


Diag.5

Diag.6



TECHNICIAN FUNCTION

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• Carriage sensor

This function allows the adjustment of the carriage sensor (diagram 8) position.

- Proceed as described below :

Move the carriage on the left until the piercingneedle stops against the piece of plastic. Pressany key : the carriage comes back in its initialposition.

The current number of steps, the mini and

maxi values are displayed as well as the wayto remove the sensor if the current value isout of ranges (see below).

CURRENT : 340 MIN : 345 MAX : 355

- For a current number of steps out of ranges,unloosen the 2 screws (see diagram 8) andgently move the sensor

- towards the right if the current value is toolow- towards the left if the current value is toohigh.

- Tighten the screws (see diagram 8) and rerun a «CARRIAGE» cycle. Check that the currentvalue is correct.

- Once entering the menu «SENSORADJUST» (Selection 6), enter the submenu «CARRIAGE SENSOR».

Diag.7

Diag.8

- Install the piece of plastic against the leftside of the tube holder compartment (seeDiagram 7).


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Diag.9

7 - Position adjustment

• Needle heigth adjustmentWhen replacing a needle, it is mandatory to adjust the heigth of the needle in the chambers.Proceed as follows :- Remove the WBC/HGB chamber cover.

- Enter the menu 7 - POSITION ADJUST / 1 - NEEDLE / 2 - ADJUSTMENT.- Manually pull down the needle until it comes into contact with the edge of the RBC chamber(Diag. 9).- Press a key to continue : the needle moves up to the initial position ; the value is stored.

• Needle motion adjustmentThe needle position in the WBC chamber can be adjusted as follows :

- Enter the menu 7 - POSITION ADJUST / 2 - CARRIAGE / 2 - ADJUSTMENT.- Position the tool (P/N : GBC 218 A) over the RBC and WBC chambers (Diag 10).- Manually lower the needle into the WBC chamber.- Press a key to continue : the needle moves up, and the carriage comes back to the initialposition ; the value is stored.- Carry out a 3 - CHECK cycle : the needle comes down to the WBC chamber.- Check that the needle is centered in the hole.

If not, enter the 2 - CARRIAGE / 1 - CARRIAGE < > menu ; the display shows the currentnumber of steps carriage motion.

If the needle goes too far on the right, add 1 step to the current value for 0.1mm.If the needle is too much on the left, decrease the current value of 1 step for 0.1mm.

Carry out a 2 - CARRIAGE / 3 - CHECK cycle again to control the needle position.

Diag.10

CARRIAGE ? : EXIT : ESCCURRENT : 893 SAVE : ENTER

TECHNICIAN FUNCTION

M I C R O

S 6 0


8 - Bubbling adjustmentAn overflow protection tank is installed on the drain circuit of each chamber. This one preventsfrom polluted liquid overflow during bubbling phasis (Diag .11).

CAUTION These values are factory adjusted (and may be different from the default valuesshown above) and should be modified only when hematologic erroneous resultsare given by the instument : If values are too important, liquid overflows canoccur or if bubbling is too low homogeneity of the dilution can be decreased.Ranges : 150 < BUBBLING 1 < 200

80 < BUBBLING 2 < 140

To modify the bubbling values, enter the menu :2 - ADJUSTMENTS / 8 - BUBBLING ADJUST. / BUBBLING 1 < >2 - ADJUSTMENTS / 8 - BUBBLING ADJUST. / BUBBLING 2 < >

and type in new step value.

Carry out a 3 - CHECK to control the adjustment.

Two bubbling phasis are adjustable :- "BUBBLING 1" is the first dilution (WBC/HGB chamber) bubbling value.- "BUBBLING 2" is the second dilution (WBC/HGB chamber + LYSE) value and RBCchamber bubbling value.

Both values correspond to a number of steps carried out by the waste/vacuum syringe.Default values are BUBBLING 1 : 175

BUBBLING 2 : 120

Diag.11



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8 - Needle sampling position

Diag.13

- Move the tube holder to one of the foursampling positions.

- Move the cursor to the function 9 -SAMPLING POSITION / 1 - NEEDLE

ADJUSTMENT.

- Close the sample holder door. The samplerassy moves to the upper position (except inthe Needle 6 position).

- Manually push the sampling needle to thebottom of the required sampling position and

press : The MICROS 60 CT adjust

automatically the needle depth whatever thetube holder position can be. The samplerassy comes back to the initial position and

the sampler door is opened.

It is possible to enter directly the required number of steps for each sampling position. Proceedas following :

Enter the sub menu that corresponds to the number of the needle : the current number of stepsis displayed. Enter the new value.

Increase the number of steps to move the needle deeper or decrease the value to raise theneedle.

Confirm the new value with . The minimum and maximum step values are as follow :

- Turn the tube holder to another needle position and carries out the same procedure to adjustthe needle depth.

NEEDLE NUMBER OF STEPS

MINI. DEFAULT MAXI.

1 1 788 1100

2 1 661 1100

3 1 612 1100

4 1 948 1100

5 1 1003 1100

6 1 845 1100

WARNING ! OEM instruments equipped with specific sample tube holders (see RAS 198 Aprocedure) must be used with the specific tubes or blood controls they were intendedfor.

TECHNICIAN FUNCTION

M I C R O

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3 - Temperature :

1 - Visible : Press to validate the temperature display.

The value displayed is the calibrated temperature.

2 - Invisible : Press to cancel the temperature display.

(Temperature invisible by default)

III - Temperature adjustment

When entering the «temperature adjustment» menu the following sub menus are displayed :

1 - Uncal. temperature

When pressing the key the sensor temperature value uncalibrated is displayed. This

value should be close to the diluent temperature.

2 - Calibrate :The temperature must be calibrated according to the diluent temperature :

- Plunge a thermometer directly into the diluent container and leave it for a while untilstabilization.

IV - Run mode

The instrument must be configurated in the «USER» mode (configurated by default in the«USER « mode), the «CONTROL» mode intends for a factory use.

V - Reagent pack

This function is used to update the instrument from a bottle mode to a pack mode.

- Run 2 diluent primes («SERVICE» menu, «PRIME» sub menu, «DILUENT» selection).

- Enter the «CALIBRATE» menu. Note the temperature of the diluent and type in the value (if itis different from the previous on the instrument).

- Press to save the new value.

NOTE For a pack equipped instrument, the thermometer must be plunged in theWBC/HGB chamber and the temperature must be note as soon as possible.

* Check

The CHECK function allows to check the piercing operation on each sampling position. Fromthe SAMPLING POSITION menu, move the cursor to the function <9> CHECK and pressENTER.Select the required position on the tube holder and close the sample door. The piercing cycleis carried out and the number of steps for this position is displayed :

NEEDLE 2CURRENT : 635 STANDARD : 630



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VI - Serial #

Displays the instrument serial number.

VII - Cycle #

Displays the : Startup number since the first use of the instrument.Stand by number since the first use of the instrument.CBC number since the first use of the instrument.

VIII - Burn-in

CAUTION This function which allows the burn-in of the instrument is intended for a factoryuse.

IMPORTANT The startup, stand by and CBC numbers are adjustable in this menu but it is mandatoryto keep the initial values (useful for maintenance schedules).



REQUIRED PRODUCTS

M

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R O S

6

0

RAS 177 A Ind.A

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


LX300 PRINTER SETUP

01/04/981 - Configuration2 - Control panel3 - Control LEDS and keys4 - Printer description

- None

- None

- None

- On request

- 15 minutes



M

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0LX300 PRINTER SETUP


PROCEDURE

NOTE On the MICROS 60, it is necessary to select the printer format RESERVED

1 of the "PRINTER" menu (function 4) accessible through the "OPTIONS"menu (function 5 of the main menu) then "RESULTS" (function 1).

Diag.1

LX300 PRINTER SETUP

M

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1 - Printer configuration :

The printer configuration is printed out when pressing the key when the printer isswitched ON. The configuration used for the MICROS 60 is the factory configuration :

Diag.2

Each parameter can be modified by the corresponding parameter chart. Each chart is acces-sible using the keys <PAUSE>, and <LF/FF> according to the control LED combinai-sons :

Diag.3



M

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0LX300 PRINTER SETUP


The control pannel keys allow the user to set up the main functions of the printer : paperadvance, paper ejection, and font selection. Control LEDS indicate the printer status.

3 - Control LEDS and keys :

LED PAUSE : The orange LED PAUSE lights when the printer stops printing. During eachpower ON, this LED blinks for few seconds and 4 audible beeps occur. When the printer runsout of paper, theLED blinks and 3 audible beeps occur. This LED lights also when the paper is in its tear off

position.When a problem occurs, this LED lights ON and 5 audible beeps occur.



Key LF/FF : During normal operation, a quick pressure on this key allows a ligne feed of thepaper. Keep the pressure on this key to feed a whole page. This key can be used to load or

eject the paper.When this key is pressed during the printer power ON, the printing test starts.

Key PAUSE : When this key is pressed during the printing, the printout stops. Press again onthis key to restart the printout.



2 - Control pannel : (See Diag.4)

Diag.4



LX300 PRINTER SETUP

M

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4 - Printer description :

Diag.5

Diag.6



REQUIRED PRODUCTS

M

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R O S

6

0

RAS 178 A Ind.A

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


LIQUID SYRINGES

16/06/98Lyse dispenser O ring replacementDiluent dispenser O ring replacementSampling needle dispenser O ring replacementLubrication of the liquid syringes

- Hexagonal keys- Dynamometric screw driver : A302 : MAG 019 A

A301 : MAG 020 A

- Silicone grease : LAM 004 A- Soft paper

- 30 min

- Grease for mechanical assemblies : XEA 381 AS

- Once a year or on request




M

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6

0LIQUID SYRINGES


PROCEDURE

NOTE In case of a leak on one of the three syringes it is recommended to replace the

entire dilution block. If not proceed as following :

- Pull the piston assy in theupper position and clamp the di-luent and lyse tubing asdescribed on the diagram 1.

- Disconnect the diluent/lyse/ sampling tubings from the 3

syringes and the tube on thesampling syringe side.

Diag.1

- Unscrew the 4 fixation screws 2 , and remove the dilution block 1 (Diagram 1).

Diag.2

- Unscrew the 6 screws 1 and 2 in

order to remove the body cover 3

(Diagram 2).

- Pull out the pistons 1, 2 and 3 from thebody with their respective o ring stillaround (Diagram 3).

- Replace the lyse and diluent O ringsby new ones. Check the cleanliness ofthe piston and of the syringe bodies. Ifnecessary clean with a soft paper.



LIQUID SYRINGES

M

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Diag.3

- Spread a little amount of siliconegrease between two fingers and applya very thin film of grease on the 2 newsampling syringe O rings. Replace the

old ones 4 .

- Reinstall the dilution block assy in thereverse order.

Use the A302 dynamometric screw

driver to tighten the screws 1 to

700mN.m (Diagram 2).

Use the A301 dynamometric screw

driver to tighten the screws 2 to

400mN.m (Diagram 2).

- Disconnect the diluent and wasteinputs located at the rear of the ins-trument (or remove the reagentpack)

- Move the liquid syringe by hand inorder to have an access to the motorgearings.

- Spread a little amount of greaseon the gearings and on the pistonaxis (Diag.4).

- Move by hand the syringeassembly to spread the grease onall parts of the gearings and piston

axis.

- Re-install the instrument cover, reconnect the waste and diluent tubes, reconnect thepower cable.

- Switch the instrument on and run several priming cycles.

• Lubrication

CAUTION Place some absorbant paper at the instrument rear connections (diluent and waste) assome liquids may come out when the syringe is pushed.



REQUIRED PRODUCTS

M

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R O S

6

0

RAS 179 A Ind.A

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


VACUUM/WASTE SYRINGE MAINTENANCE

16/06/98- O ring replacement

- Hexagonal keys- Dynamometric screw driver A302 : MAG 019 A

- Silicone grease : LAM 004 A- Grease for mechanical assemblies : XEA 381 AS.

- 20 min

- See maintenance chart table




M

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0VACUUM/WASTE SYRINGE MAINTENANCE


PROCEDURE

- Manually pull the syringe piston up in order to freed it from the syringe body.

NOTE The syringe has to be linked to the atmosphere, pressing the valve

, to pull the piston out from the syringe body.

Turn the cylindrical gearing by hand to help the raising of the

piston (See Diagram 1).

Diag.1



M

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6 0


VACUUM/WASTE SYRINGE MAINTENANCE

- Unscrew the fixation screws in order to remove the syringe body.

- Unscrew the O ring tightening screws and remove the O ring .

- Spread a little amount of silicone grease between two fingers and apply a very thin film ofgrease on a new O ring.

- Reinstall in the reverse order. Apply the following torque to the screws : 700 mN.m.

- Check the watertightness of the syringe running a «CHECK PRESSURE» cycle (see procedure

: RAS 172 A for OT or RAS 176 A for CT).

- Run cycles and check for correct operations.

CAUTION Push the piston back inside the syringe body before tightening the o ring

fixation screws . Use the dynamometric screw driver to tighten the screws

to 400 mN.m

- Switch off the instrument and remove thepower cable.

- Disconnect the diluent and waste inputs locatedat the rear of the instrument.

- Using a small and flat screwdriver, spread alittle amount of grease on the gearings of theair syringe reductor plate (Diag.2).

Diag.2

- Spread a little amount of grease on the coggsof the piston axis (Diag.3).

Diag.3

• Lubrication



M

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0VACUUM/WASTE SYRINGE MAINTENANCE


- Move by hand the piston axis up and down in orderto spread the grease all around the gearings andalong the axis (Diag.4).

Diag.4



REQUIRED PRODUCTS

M

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R O S

6

0

RAS 180 A Ind.A

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


INSTRUMENT LANGUAGE

01/04/98Changing the instrument language

Pair of pliers

None

5 minutes

On request only

None



M

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6

0INSTRUMENT LANGUAGE


PROCEDURE



INSTRUMENT LANGUAGE

M

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6 0


- Replug the jumpers on E1 according to the wished language :

JUMPER CONFIGURATION ON E1

- Switch on the instrument.

ENGLISH

FRENCH

SPANISH

GERMAN



REQUIRED PRODUCTS

M

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R O S

6

0

RAS 181 A Ind.A

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


REAGENT PACK

10/04/98- Replacement of the waste connector O ring- Replacement of the diluent/clean/lyse O rings

- Pair of pliers- Torx keys

- None

- O ring replacements : 1/year

- O rings : FAA 036 A

- 15 min



M

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0REAGENT PACK


PROCEDURE

- Disconnect the pack if this one is still connected.

- Dismantle the reagent pack front panel unscrewing the four torx screws as shown on the

diagram 1.

- Use a pair of pliers to cut the O rings of the reagent connectors (diag 2) .

- Replace the O rings by new ones.

- Replace as well the waste connector O ring (diag 3).

Diag.1

Diag.2 Diag.3



REQUIRED PRODUCTS

M

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R O S

6

0

RAS 182 A Ind.A

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


BARCODE READER

20/04/98Barcode reader installation & configuration

None.

None.

10 minutes.

On request.

Installation kit : XBA 379 AS



M

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6

0BARCODE READER


PROCEDURE

Installation :

- Switch off the instrument.- Open the instrument cover.

- Install the jumper (included in the XBA 379 AS kit) on E10 as shown on the diagram 3.

- Connect the BARCODE reader in the DIN plug of the RS adaptor wiring (diag 1 and 2).

Setup :

- Switch on the instrument and within the15 first seconds, read from the top to thebottom the 3 barcode labels located onthe top left of the page 4 of this procedure.The audible beep occurs after eachreading.

- After the 3 labels (the audible signalbeeps 5 times) read all the labels fromtop to bottom and from left to right.

- When the last label is read, the signalbeeps 5 times in order to indicate the endof the setup. Check on the test labelslocated on the last page that the readingis correct.

NOTE - In case of difficulties to read 2 consecutive 0, move away the barcodereader from the page after the first reading and then read the second 0.- To carry out the read test after setup the instrument must be configurated ina US mode in order to obtain the barcode identification in the ID field (menu 5- SETUP / 3 - SPECIAL / 7- ID MODE).

- The barcode reader can be configurated according to the type of barcodelabel in use (from the menu 5 - SETUP / 6 - BARCODE) and allows to enablethe checksum or not.The barcode setup can be printed out by the function 5 - SETUP / 3 - SPE-CIAL / 5 - PRINT CONFIG.

Diag.1

Diag.2

WARNING ! Once the jumper installed on E10, the instrument data ouput receives 5 volts to supplythe barcode reader. This voltage should cause damages on computer connection ifthis one is directly connected on the MICROS 60 data ouput. It is then mandatory toconnect the computer connection only on the cable (DAC 023 AS shown on the diagram2) intended for it.



BARCODE READER

M

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Diag.3



M

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0BARCODE READER


Within 15 seconds after switch on :

(5 beeps)

Setup : default setting

Parity : even

1 bit stop

Code identifier : no

Codabar : yes

No start/stop code

No STF

No C93

C128

Ø

Ø

Enter

Exit

5 Beeps



BARCODE READER

M

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6 0


WITHOUT CHECKSUM

C39

TEST

2/5

9076543210

CODABAR

543210

C 128

98765

WITH CHECKSUM

EAN 8

1234567

EAN 8

1234567

EAN 13

123456789012

EAN 13

123456789012

READ TEST AFTER SETUP



REQUIRED PRODUCTS

M

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R O S

6 0 O

T

RAS 187 A Ind.A

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


HYDRAULIC CYCLE CHECKUP

16/04/98

Step by step control of the hydraulic cycle.

None

Blood samples

15 minutes

On request

None



M

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6 0 O

THYDRAULIC CYCLE CHECKUP


PROCEDURE

Diag.1

Diag.2

123

4

5

1 - Cycle start condition

• Needle 1 in the sampling position (diag 1).

• RBC chamber 2 filled with 2.5ml of diluent.

• WBC/HGB chamber 3 filled with 2.5ml of diluent.

• Liquid syringes 4 in standby position.

• Vacuum/waste syringe 5 in the lower position.

2 - Sampling

• Aspiration of 10µl of blood sample

Causes

The liquid syringes assembly moves down (diag

2) and pulls down the micro sampling syringe 1 .

123



HYDRAULIC CYCLE CHECKUP M I C

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• Sampling carriage transfer over the WBC/HGB chamber.• Counting head rinse

• WBC/HGB chamber drain

• Needle motion downward of a few steps (Diag 5)• Diluent is delivered from the rinsing block

Diag.3

3 - Outer sampling needle rinse

Causes - The sampling needle moves up.

- The liquid syringes 2 (diag 2 ) send diluent for rinse

through the rinsing block - Polluted diluent is aspirated (from the lower to the upper tube) by means of waste/Vacuum syringe raise.

Diag.4

4 - WBC/HGB chamber rinse & HGB blank measure

Diag.5

Causes Diluent is delivered by means of the liquid syringes raise

Aspiration by means of the Va- cuum/waste syringe raise (diag 4)

The liquid syringes move up and a flow of diluent is delivered to the chamber via the outer needle.



M

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• WBC/HGB chamber drain (second time)• Diluent is delivered from the outer needle (second time)• HGB blank measure (beep triggered)• RBC and WBC/HGB chamber drains.

5 - Dilutions

• Sampling needle moves down to the WBC/HGB chamber• Injection of 1.7ml of diluent into the WBC/HGB chamber+ Injection of 10µl of blood sample

• Bubbling

Causes

Raise of the liquide syringes (diag 6) : - delivers 0.5ml of diluent from the outer sampling needle - injects 1.2ml of diluent + blood sample from the inner sampling needle

Bubbling by means of vacuum/ waste syringe downward motion (Diag 7).

Causes The liquid syringes move down and pull down the micro sampling syringe (diag. 8)

• Sampling needle moves up• Outer needle short aspiration (dries the needle)• Sampling needle moves back in the chamber• Aspiration of 28.3µl of diluted blood (dilution 1/170)

Diag.7

Diag.6

Diag.8

1 2

• Sampling needle moves up• Injection of 0.4 ml of diluent into the WBC/HGB chamber

Causes Raise of the liquide syringes (diag.6) : - delivers 0.4ml of diluent from the outer sampling needle



HYDRAULIC CYCLE CHECKUP M I C

R O

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• Outer needle aspiration• Carriage motion over the RBC chamber• Sampling needle moves down into the RBC chamber• Lyse injection into the WBC/HGB chamber Causes

The liquid syringes raise (diag 8)

and the syringe 1 delivers

0.52ml of lyse via the WBC chamber bottom

• Injection of 2.5 ml of diluent into the RBC chamber+ Injection of 28.3 µl of diluted blood

Diag.8 Diag.9

• Bubbling (diag 10)• Sampling needle moves up• Carriage motion over the WBC/HGB chamber• Counting head rinse

Causes The liquid syringes raise (diag 8) : - delivers 0.5ml diluent via the outer sampling needle - injects diluted blood from the inner needle + 2ml of diluent

Diag.10

1 2

FINAL DILUTION in the WBC CHAMBER :1.7ml + 0.4ml diluent + 0.52ml of lyse = 1/260

FINAL DILUTION in the RBC CHAMBER :28.3µl of diluted blood at 1/170 + 2.5ml diluent

1

170

2833

2500

1

15000* =

.

M

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NOTE A third count (C3) is carried out if the difference between first (C1) and se-cond count (C2) is not within acceptable limits :

- WBC :if C1 or C2 > 3000C3 is carried out if difference between C1 and C2 > 7%

if Max C1 or C2 < 3000C3 is carried out if difference between C1 and C2 > 9%

- RBC :

if C1 or C2 > 16000C3 is carried out if difference between C1 and C2 > 5%


- PLT :if C1 or C2 > 400C3 is carried out if difference between C1 and C2 > 15%


• Counting head rinse• WBC chamber drain• Diluent injection into the WBC chamber from the outer needle• RBC chamber drain• Carriage motion over the RBC chamber• Diluent injection into the RBC chamber from the outer needle (diag 11)

6 - Counts• First counts (beep triggered)• Counting head rinse• Second counts (beep triggered)

• Carriage & needle motions back to the initial positions• Results display and printed out

Diag.11



REQUIRED PRODUCTS

M I C

R O S 6 0 C T

RAS 188 A Ind.A

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


HYDRAULIC CYCLE CHECKUP

02/06/98

Step by step control of the hydraulic cycle.

None

Blood samples

15 minutes

On request

None



M

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THydraulic cycle checkup


PROCEDURE

Diag.1

1

23

4

5

1 - Cycle start condition

• Needle 1 in the sampling position (diag 1 & 2).

• RBC chamber 2 filled with 2.5ml of diluent.

• WBC/HGB chamber 3 filled with 2.5ml of diluent.

• Liquid syringes 4 in standby position.

• Vacuum/waste syringe 5 in the lower position.

2 - Cap piercing• Carriage motion over the WBC chamber.• WBC/HGB chamber drain

• Diluent injection into the WBC/HGB chamberthrough the channels (A) and (B) (see diag.4)

• HGB blank measure (beep triggered)• Carriage return over the piercing device• Atmosphere is provided inside the tube

• Cap piercing

Causes Aspiration by means of the Va- cuum/waste syringe raise.

Diluent is delivered by means of the liquid syringes raise

Causes Liquid valve #3 is activated

Sampling holder rises in the upper position.The needle pierces the tube cap

1

Diag.2



Hydraulic cycle checkup

M I C R O

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3 - Sampling

A

B B

C

• Sampling needle (diag. 2) moves down to the lower position (inside the tube)• Aspiration of 10µl of blood sample

Causes The liquid syringes assembly moves down (diag

3) and pulls down the micro sampling syringe 1 .

Diag.3

• The sampling needle comes back in the upper position

• RBC chamber drain• Counting head rinse• Carriage motion over the WBC/HGB chamber• Sampling holder door opens• WBC/HGB chamber drain

Diag.4

4 - needle rinses

Causes

- The liquid syringe 2 (diag 3) sends diluent for rinse

through the outer piercing needle (A) (diag 4) and inner piercing needle (B). The polluted diluent is sent to the WBC/HGB chamber.

NOTE The piercing needle inner rinse is equivalent to the sampling needle outerrinse.

• WBC/HGB chamber drain• Second needle rinses• WBC/HGB chamber drain• The sampling needle moves down into the WBC/HGB chamber

123

A - Piercing needle outer channelB - Sampling needle outer channelC - Sampling needle inner channel

Diag.5



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5 - Dilutions

• Injection of 1.7ml of diluent into the WBC/HGB chamber+ Injection of 10µl of blood sample

• Bubbling

• Sampling needle moves up• Outer needle short aspiration (dries the needle)

Causes Raise of the liquide syringes (diag 7) : - delivers 0.5ml of diluent from the outer sampling needle (channel B diag.6 )

- injects 1.2ml of diluent + blood sample from the inner sampling needle (channel C)

Bubbling by means of vacuum/ waste syringe downward motion (Diag 8).

• Sampling needle moves back in the chamber• Aspiration of 28.3µl of diluted blood (dilution at 1/170)

• Sampling needle moves up

• Injection of 0.4 ml of diluent into the WBC/HGB chamber

• Carriage motion over the RBC chamber• Sampling needle moves down to the RBC chamber

• Lyse injection into the WBC chamber + Bubbling

Diag.8

Causes Raise of the liquide syringes (diag.7) : - delivers 0.4ml of diluent from the outer sampling needle (channel B)

FINAL DILUTION in the WBC CHAMBER :1.7ml + 0.4ml diluent + 0.52ml of lyse = 1/260

Causes The liquid syringes raise (diag.7)

and the syringe 1 delivers

0.52ml of lyse via the WBC

chamber bottom

A

B B

C

Diag.6 Diag.7

1



Hydraulic cycle checkup

M I C R O

S 6 0


• Injection of 2.5 ml of diluent into the RBC chamber+ Injection 28.3 µl of diluted blood

Causes The liquid syringes raise (diag.9) : - delivers 0.5ml diluent via the outer sampling needle (channel B diag. 6)- injects diluted blood from the inner

needle + 2ml of diluent (channel C)

• Bubbling (diag 11)• Sampling needle moves up• Carriage motion over the WBC/HGB chamber• Counting head rinse

Diag.9

Diag.11

Diag.10

FINAL DILUTION in the RBC CHAMBER :28.3µl of diluted blood at 1/170 + 2.5ml diluent

1

170

2833

2500

1

15000* =

.



M

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NOTE A third count (C3) is carried out if the difference between first (C1) and se-cond count (C2) is not within acceptable limits :

- WBC :if C1 or C2 > 3000C3 is carried out if difference between C1 and C2 > 7%


- RBC :

if C1 or C2 > 16000C3 is carried out if difference between C1 and C2 > 5%


- PLT :if C1 or C2 > 400C3 is carried out if difference between C1 and C2 > 15%


• Counting head rinse• WBC chamber drain• Diluent injection into the WBC chamber from the outer sampling needle (channel B)• RBC chamber drain• Carriage motion over the RBC chamber• Diluent injection into the RBC chamber from the outer sampling needle (channel B)• Carriage & needle motions back to the initial positions

6 - Counts• First counts (beep triggered)• Counting head rinse• Second counts (beep triggered)

• Results display and printed out

REQUIRED PRODUCTS

M

I C

R O S

6

0

RAS 191 A Ind.B

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


OVERALL MAINTENANCE

05/10/98Instrument maintenance step by step

Hexagonal keysDynamometric screw driver A302, A301, A300ClampsScalpel

Cutting pliersPair of scisors

VoltmeterFlat screw driverBarflexThermometer

Torx keys

Empty sample tubesSilicone greaseSoft tissueLiquid soapDistilled waterMicropipette tip

Flat piece of stiff plasticLatex WBC, RBCFelt pensyringe 5mlFresh blood samplesCalibratorCommercial control

2 h 30

Spare parts kit : XEA 458 ASNeedle position tool : GBC 218 A

The yearly maintenance frequencies vary with the instrument output.

According to the cycle numbers, 3 categories are created :< 6000 cycles/year -> 1 overall maintenances/year6000 to 15000 cycles/year -> 2 overall maintenances/year> 15000 cycles/year -> 3 overall maintenances/year



M

I C R O S

6

0OVERALL MAINTENANCE


1 - Reagent checkü Type of reagent usedü Expiration dates

ü Levelsü Pack : Number of cycles left

2 - Operation checkü Blank cycle controlü QC controlü Fresh blood sample runü Calibration coefficient checkupü Leak control and general cleanliness of the instrument

B - CLEANING AND MAINTENANCE

1 - Chamber maintenanceü RBC & WBC/HGB chamber cleaningü Aperture checkü Coaxial O ring replacementü Aperture O ring replacement

Aperture tightening torque : 100mN.m

2 - Liquid syringesü Cleaningü O ring replacement (Lyse, diluent, Micro syringe)Torque values :

1 torx screws : 400mN.m

2 Hexagonal screws : 700mN.m

ü Lubrication of the gearings and piston axis

3 - Sampling needleü Cleaningü Sampling needle O ring replacement

CT : O ring holder tightening torque : 700mN.mOT : O ring holder tightening torque : 100mN.m

ü Free pulley cleaning and lubrication

Pulley tightening torque : 400 mN.mü Piercing needle check/cleaning (CT)

4 - Air syringeü Cleaningü O ring replacement

O ring tightening torque : 400mN.mSyringe holding torque : 700mN.m

ü Lubrication of the gearings and piston axis

5 - Piercing block maintenanceü Check and clean

6 - Reagent pack (Option)ü Replacement of the connector O rings

Procedures

RAS 169 A

RAS 178 A

RAS 167 A (CT)RAS 168 A (OT)

RAS 179 A

RAS 198 A (CT)

RAS 181 A

A - INSTRUMENT CHECKUP

Diag.1

OVERALL MAINTENANCE

M

I

C R

O S

6 0


1 - Liquid syringesü Operation check

Menu 4 - SERVICE / 5 - MECHANIC / 4 - LIQ. SYRINGES

C - MECHANICAL OPERATION CHECK

2 - Air syringeü Operation check

Menu 4 - SERVICE / 5 - MECHANIC / 5 - PRESSURE SYR.

3 - Liquid valvesü Operation check

Menu 4 - SERVICE / 5 - MECHANIC / 6 - VALVES

4 - Sampling needle and carriageü Operation check

Menu 4 - SERVICE / 5 - MECHANIC / 2 - NEEDLE U/DMenu 4 - SERVICE / 5 - MECHANIC / 3 - CARRIAGE L/R

D - MECHANICAL ADJUSTMENTS

1 - Needle heigth ü Menu 7 - TECHNICIAN / 2 - ADJUSTMENTS / 7 - POSITION ADJST. / 1- NEEDLE / 2 - ADJUSTMENT

ü Needle on the edge of the RBC chamber, press a key.

2 - Needle motionü Tool GBC 218 A over the RBC and WBC chamber.ü Menu 7 - TECHNICIAN / 2 - ADJUSTMENTS / 7 - POSITION ADJST. / 2 -

CARRIAGE / 2 - ADJUSTMENT

ü Lower the needle in the WBC chamber , press a key.

E - HYDRAULIC ADJUSTMENTSü PRIME / ALL REAGENTSü Check for leaks

1 - Check vacuumü Barflex connected instead of the tube coming from the valve 8.ü Menu SERVICE / 7 - TECHNICIAN / 2 - ADJUSTMENTS / 3 - CHECK PRESSURE

Value : -200mbar + 10mbarü Check vacuum drop down during 30 secondes < 2 mbar

2 - Bubblingü Menu 7 - TECHNICIAN / 2 - ADJUSTMENTS / 8 - BUBBLING ADJUST. / BUBBLING 1 < > (default value : 175)

ü Menu 7 - TECHNICIAN / 2 - ADJUSTMENTS / 8 - BUBBLING ADJUST. / BUBBLING 2 < > (default value : 120)

3 - Drain detectionü Voltmeter between TP30 and TP6ü

Drained sensor : adjust the voltage to 4,5V + 0,3V by means of R6ü Fill the sensor with diluent : voltage falls down below 1Volt

Procedures

RAS 173 A

RAS 172 A (OT)RAS 176 A (CT)




RAS 174 A



M

I C R O S

6



5 - Photometer calibrationü Menu 7 - TECHNICIAN / 2 - ADJUSTMENTS / 1 - CAL PHOTOMETER

ü Hgb channel displayed on the screen, adjusted by means of R97,according to the room temperature :

ROOM

TPT

(°C)

CHANNEL ROOM

TPT

(°C)

CHANNEL

Mini. Nominal Maxi. Mini. Nominal Maxi.

18 238 243 248 24 233 238 243

19 237 242 247 25 232 237 242

20 236 241 246 26 231 236 241

21 235 240 245 27 230 235 240

22 234 239 244 28 229 234 239

23 234 239 244 29 228 233 238

4 - Temperatureü Thermometer plunged into the diluent container (WBC chamber if pack)ü Menu 7 - TECHNICIAN / 3 - TEMPERATURE / 2 - CALIBRATEü Temperature diluent = temperature displayed + 2°

IMPORTANT Temperature adjustment must be done 20 minutes at least after the instru-ment has been switched on.

Procedures





6 - Gains• WBCü Latex thoroughly mixedü Blank cycle to check the cleanlinessü Menu 7 - TECHNICIAN / 2 - ADJUST. / 4 - WBC ADJUST / 1 - WBC ADJUSTü Run an analysis on Latex. Adjust with R74 to obtain

- LYM = 57 + 1- GRA = 180 + 2

• RBCü Latex thoroughly mixedü Blank cycle to check the cleanlinessü Menu 7 - TECHNICIAN / 2 - ADJUST. / 5 - RBC PLT ADJUST / 1 - RBC PLT ADJUSTü Run an analysis on Latex.ü Adjust :

- the RBC gain with R81 to obtain RBC = 74 + 1- The PLT gain with R87 to obtain PLT = 59 + 2



OVERALL MAINTENANCE

M

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C R

O S

6 0


F - VOLTAGE CHECKS

1 - Power supplyü Ground on TP31, TP30 or TP29

Procedures

RAS 171 A

RAS 175 A

RAS 175 A

RAS 172 A OTRAS 176 A CT

TEST POINTS VOLTAGE

TP 20 -12V + 0,5V

TP 22 24V + 1.5V - 0V

TP 23 5V + 0,3V - 0V

TP21 12V + 0,5V

2 - RBC, PLT, WBC thresholdü Ground on TP31

THRESHOLDS TEST POINTS VOLTAGE POTENTIOMETERS

WBC TP 10 350 mV + 7 R68

RBC TP13 400 mV + 7 R75

PLT TP16 180 mV + 3 R82

3 - Stepper motor voltageü Ground on TP31

M OTO RS TEST PO INTS VO LTA GE POTEN TIO .

Air syringe TP36 2.50 V + 0.05 V R195

Liquid syringe TP35 2.00 V + 0.05 V R186

Horizontal carriage TP37 1.50 V + 0.05 V R177

Vertical carriage TP38 1.00 V + 0.05 V R168

4 - Aperture voltageü Ground on TP30üMenu 4 - SERVICE / 7 - TECHNICIAN / 2 - ADJUSTMENTS /

2 - APERTURE VOLTAGEü Test point on TP 19 : check to have 60V +2.8V -1.5V

M

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6



G - BLOOD SAMPLE RUN

1 - Preliminaryü Run a STARTUP cycle

2 - Repeatabilityü Fresh and normal blood sampleü Run 20 consecutives analyses

H - CALIBRATIONü Calibration passed :Check that the calibration coefficients remain within the following ranges :

PARAMETERS % CV TEST LEVEL

- WBC : < 2,5% at 10.109 /l

- RBC : < 2% at 5.1012 /l

- HGB : < 1,5% at 15 g/dl

- HCT : < 2% at 45 %

- MCV : < 1% at 90 fl

- PLT : < 5% at 300.109 /l

- LYM : < 5% at 40%(16/18 param.)- MON : < 10% at 10% (16/18 param.)

- GRA : < 5% at 50% (16/18 param.)

LIMITS WBC RBC HGB HCT PLT MPV RDW PDW

MINIMUM 0.80 0.65 1.10 0.83 0.86 0.75 0.75 0.75

MEAN 1.00 0.81 1.38 1.04 1.07 0.94 1.00 1.00

MAXIMUM 1.20 0.97 1.66 1.25 1.28 1.13 1.25 1.25

I - QUALITY CONTROLü Run a blood control

ü Option : QC smart card use

X Xi

n=

∑ ( )SD

X Xi

n=

−

−

∑2

1

X : Mean

Xi : measure valuen : Measure number

SD : Standard deviation

CV SD

X (%) = ×100

CV calculated by means of the belowformula :

ü Control to have variation coefficients within the following acceptable limits :

CAUTION For MICROS 60 CT, it is mandatory to remove the cap from the sampletube to prevent from piercing several times the same cap.



OVERALL MAINTENANCE

M

I

C R

O S

6 0




REQUIRED PRODUCTS

M

I C

R O S

6

0

RAS 192 A Ind.A

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


DECONTAMINATION

01/05/98

Hexagonal keysClampsFlat screw driverTorx keys

Drain and rinse kit XEA 349 AS for Pack equipped instrument

Fungicidal, bactericidal, virus killing detergent spray, non corrosive for metals,Non plastic altering.Bleach solution 12°ClDeionize waterProtection glovesAbsorbant paperDistilled water

1h35min

On request

Instrument decontamination before maintenance operation in the following cases- Instrument move out of the biologic risks area- Maintenance intervention on contaminate suspected assemblies



M

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R O S

6

0DECONTAMINATION


PROCEDURE

WARNING ! - Disposal gloves and white coat must be worn by the operator.- Local or national regulations must be applied in all the operations .

1 - Preliminary (20min)- Switch on the instrument- Run a STARTUP cycle, then a SERVICE / AUTOCLEAN- Switch off the instrument and remove the supplying cable- Open the instrument cover

- Spray the bactericidal cleaner on all assemblies that may provide biologic risks and waitfor 10 minutes (assemblies in contact with the operator such as instrument cover, tubeholder, keyboard, start key, sampling needle neighboured assemblies...

2 - Manual decontamination (20 min)- Remove the WBC/HGB chamber cover- Dilute the 12°cl bleach to 1 part of bleach for 4 of deionize water (1/5).- Instrument environment must be cleaned and decontaminated.- No sponge, nor cloth must be used. Only absorbant paper, thrown after use, in contami-nation bins, can be employed. For small or weak assemblies use accurate drier papers.- All assemblies that is suspected to have contact with biologic product must be disinfectedwith the diluted bleach (the stainless steel must bleached below 30°Celsius).- Blood stains or salt marks must be cleaned with spray detergent first.

• Concerned assemblies

- Outer surfaces of the instrument (perpex, covers, LCD, reagent locations....)- Keyboards- Waste connector plug- Liquid valve push- Needle neighboured assemblies- Tube holder assy.- overflow trays

Reinstall all the assemblies and setup the instrument in its initial configuration.

3 - Analysis circuit decontamination (30 min)• BOTTLE VERSION

- Prepair 1 bottle containing 1/2 litre of bleach diluted to 1 part of bleach for 9 parts ofdeionize water (1/10).- Prepair 1 bottle containing 1/2 litre of distilled water.- Switch on the instrument- Replace the reagent bottles by the diluted bleach bottle.- Run a SERVICE / PRIME / ALL REAGENTS cycle.- Fill a sample tube with diluted bleach to 1 part of bleach for 4 of deionize water (1/5).- Enter the TECHNICIAN / BURN-IN function, Type in 15 cycles and leave the instrument

operating until it stops (On MICROS 60 OT run 15 manual cycles).



DECONTAMINATION

M

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C R

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6 0


4 - Drain and rinse (30 min)- Remove the 3 reagent straws from the bottle containing the diluted bleach

- Wrap the straws in absorbant paper.- Run two prime cycles : the bleach is drained.- Replace the diluted bleach by the distilled water bottle and re-plunge the straws indistilled water.- Run six PRIME / ALL REAGENTS cycles (Rinse).- Remove the 3 reagent straws from the distilled water (Wrap the straws in absorbantpaper).- Run two PRIME / ALL REAGENTS cycles : the distilled water is drained.- Run a STAND BY cycle.- Re-install the reagent bottles and the straws (or re-install the Pack instead of the Drain& Rinse kit).- Switch off the instrument.

- Close the instrument cover.

• PACK VERSION - Prepair 1 bottle containing 1/2 litre of bleach diluted to 1 part of bleach for 9 parts ofdeionize water (1/10).- Prepair 1 bottle containing 1/2 litre of distilled water.- Prepair one empty bottle of 1 litre for waste.

- Switch on the instrument- Replace the reagent pack by the Drain & Rinse kit (XEA 349 AS).- Plunge the straws into the diluted bleach bottle and the waste tube into the empty wastebottle.- Run a SERVICE / PACK / PRIME cycle.- Fill a sample tube with diluted bleach to 1 part of bleach for 4 of deionize water (1/5).- Enter the TECHNICIAN / BURN-IN function, Type in 15 cycles and leave the instrumentoperating until it stops (On MICROS 60 OT run 15 manual cycles).



REQUIRED PRODUCTS

M

I C

R O S

6

0

RAS 197 A Ind.B

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


DRAIN & RINSE

05/10/98

None

Drain & rinse kit : XEA 349 AS for Pack equipped instrumentADVIA Pack optional : Reagent output protections FFZ 015 A

Distilled water

35min

On request

Instrument Rinse and drain before- an extended shutdown- an instrument removing



M

I C

R O S

6

0DRAIN & RINSE


PROCEDURE

WARNING ! - Disposal gloves and white coat must be worn by the operator.- Local or national regulations must be applied in all the operations .

1 - Preliminary (5min)- Switch on the instrument- Run a STARTUP cycle, then a SERVICE / AUTOCLEAN

2 - Drain and rinse (30 min)

• BOTTLE VERSION

- Prepair one bottle containing 1/2 litre of distilled water- Remove the 3 reagent straws MINIDIL, MINILYSE, MINICLEAN from the bottles.- Wrap the straws in absorbant paper.- Run two PRIME / ALL REAGENTS cycles : the reagents are drained.- Replace the reagent bottles by the distilled water bottle and plunge the straws into distilledwater.- Run 6 PRIME / ALL REAGENTS cycles (Rinse).- Remove the 3 reagent straws from the distilled water (Wrap the straws in absorbant paper).- Run two PRIME / ALL REAGENTS cycles : the distilled water is drained.- Run a STAND BY cycle.- Check that the diluent syringe piston is in park position (upper position).- Remove the distilled water and install the installation kit box instead.- Install the black plastic carriage locking clip in order to block the needle carriage (see INS-TALLATION procedures :

MICROS 60 CT : RAS 166 AMICROS 60 OT : RAS 165 A)

- Clean the reagent stains from the instrument.- Put an adhesive tape on the tube holder door (MICROS 60 CT) to prevent from opening it.- Switch the instrument off.



DRAIN & RINSE

M

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6 0


• PACK VERSION - Prepair one bottle containing 1/2 litre of distilled water.- Prepair one empty bottle of 1/2 litre for waste.- Install the Drain & Rinse kit (XEA 349 AS) instead of the Reagent pack.- Plunge the waste tube into the empty waste bottle.

- Wrap the 3 reagent straws MINIDIL, MINILYSE, MINICLEAN in absorbant paper.- Run two SERVICE / PACK / PRIME cycles : the reagents are drained.- Plunge the straws into distilled water.- Run 6 SERVICE / PACK / PRIME cycles (Rinse).- Remove the 3 reagent straws from the distilled water (Wrap the straws in absorbantpaper).- Run two SERVICE / PACK / PRIME cycles : the distilled water is drained.- Run a STAND BY cycle.- Check that the diluent syringe piston is in park position (upper position).- Remove the Drain & rinse kit.- Switch off the instrument.- Install the black plastic carriage locking clip in order to block the needle carriage (seeINSTALLATION procedures :

MICROS 60 CT : RAS 166 AMICROS 60 OT : RAS 165 A)

- Clean the reagent stains from the instrument.- Put an adhesive tape on the tube holder door (MICROS 60 CT) to prevent from openingit.- Switch the instrument off.

NOTE

- Install the installation kit box on the pack location.

ADVIA : Install the reagent ouput protections (see the warning sheet form :RAL 035 A and join it to the installation kit box)



REQUIRED PRODUCTS

M I C

R O S 6 0 C T

RAS 198 A Ind.A

CONCERNS

REQUIRED TOOLS

INTERVENTION TIME

FREQUENCY


Piercing block maintenance

15/06/98- Sampling position and available tubes- Piercing block description- Maintenance

- Torx keys

- See decontamination procedure : RAS 192 A

- 30 min

- On request

- None



M

I C

R O S 6

0 C

TPiercing block maintenance


PROCEDURE

1 - Sampling position

According to the sample tube models (Vacutainers,Microtainers ...) used and to their position into the sample

tube holder, the heigth of the needle in its lower positioncan be modified.

The 3 switches (diag 1) associated to the tube holderare able to detect the sampling position according tothe following principle :

Two states 0/1 are possible for the 3 switches (see diag2) :

- Switched OFF : 1- Switched ON : 0

The binary codes obtained from the states of theswitches gives the positions of the tube holder.

For each position of the sample tube holder (from 1 to6) corresponds a position of the needle (from 1 to 6).

Diag.2

SWITCH

1

SWITCH

2

SWITCH

3Sampling position Needle

0 0 0Bad position of the tube

holder

1 0 0 position 1 Needle 1






1 1 1 No tube holder

NOTE The code «0 0 0» means that the tube holder has been turned in betweentwo sampling positions. The code «1 1 1» means that the tube holder hasbeen removed.

Diag.1




M I C R O

S 6 0


The following chart tables give the tube positions available according to the models of sampleholder used on the instrument

MICROS 60 STANDARD TUBE HOLDER

Barrillet

standard

(GBC061AS)

Standard

Tube holder

Marque

Trademark

Additif

Additive

Volume

Volume

Vide

Vacuum

Volume mort

(5)

Dead volume

Ref.

P/N

Modèle

Model

Photo N°

Photo #

Réglage

(1)

Adjustment

Perçage tube

Tube piercing

Bouchon

(2)

Stopper

POSITION 6

CONTROLE

R&D

systemna 2ml na 250µl na na 8 standard

sans bouchon

without stopper

POSITION 5

STANDARD

Becton D. EDTA-K3 5ml nc 390µl 368452 Vacutainer 9 standardavec bouchon

with stopper

gomme

rubber

Becton D. EDTA-K3 5ml 2ml 400µl 367651 Vacutainer 11 standardavec bouchon

with stopperhemogard





Becton D. EDTA-K3 5ml 4.5ml 410µl 367654 Vacutainer 7 standardavec bouchon


Terumo EDTA-K2 5ml 3ml 430µl VP-053SDK Venoject II 15 standardavec bouchon

with stopperUltraseal

Terumo EDTA-K3 5ml 5ml 460µl VT-050STK Venoject 17 standardavec bouchon

with stopper

gomme

rubber

Terumo EDTA-K3 5ml 3ml 460µl (4) VT-053STK Venoject na standardavec bouchon

with stopper

gomme

rubber

ABX EDTA-K3 5ml 4ml 480µl ABX-3004002 na 14 standardavec bouchon

with stopper

gomme (non recommandé)

rubber (not recommended)

Greiner EDTA-K3 5ml 2ml 370µl (4) 454087 Vacuette na standardavec bouchon




Greiner EDTA-K3 5ml 4ml 370µl 454036 Vacuette 13 standardavec bouchon


LDM EDTA-KE 5ml 4.5ml 480µl nc nc 2 standardavec bouchon


POSITION 4

Becton D. EDTA-K3 3ml na 30µl 6385 Vacutainer 6 standard

sans bouchon

(3)

without stopper




with stopper



POSITION 2

Sarstedt nc 0.5ml na 30µl 901091 nc 4 standardsans bouchon

without stopper

bouchon imperdable

unlostable stopper

ABX nc 0.5ml na 30µl ABX-3001001 nc 5 standardsans bouchon

without stopper

bouchon imperdable

unlostable stopper

Table 1

Diag.3

Diag.4

CAUTION The needle sampling position must not be modified without refering to the procedureRAS 176 A.



M

I C

R O S 6

0 C



Barrillet

standard

(GBC216AS)

Standard

Tube holder

Marque

Trademark

Additif

Additive

Volume

Volume

Vide

Vacuum

Volume mort

(5)

Dead volume

Ref.

P/N

Modèle

Model

Photo N°

Photo #

Réglage

(1)

Adjustment

Perçage tube

Tube piercing

Bouchon

(2)

Stopper

POSITION 6

CONTROLE

STRECK nc nc nc nc nc nc 18 standardavec bouchon

with stoppernc

R&D


sans bouchon

without stopper

POSITION 5

STANDARD


with stopper

gomme

rubber

Becton D. EDTA-K3 5ml 2ml 400µl 367651 Vacutainer 11 standard avec bouchon








Terumo EDTA-K2 5ml 3ml 430µl VP-053SDK Venoject I I 15 standardavec bouchon

with stopperUltraseal


with stopper

gomme

rubber


with stopper

gomme

rubber

ABX EDTA-K3 5ml 4ml 480µl ABX-3004002 na 14 standardavec bouchon

with stopper






with stopper

hemogard

Greiner EDTA-K3 5ml 4ml 370µl 454036 Vacuette 13 standardavec bouchon




POSITION 4

Becton D. EDTA-K3 3ml na 30µl 6385 Vacutainer 6 standard

sans bouchon

(3)

without stopper




with stopper



POSITION 2

Sarstedt nc 0.5ml na 30µl 901091 nc 4 standardsans bouchon

without stopper

bouchon imperdable

unlostable stopper

ABX nc 0.5ml na 30µl ABX-3001001 nc 5 standardsans bouchon

without stopper

bouchon imperdable

unlostable stopper

ADVIA 60 STANDARD TUBE HOLDER

Table 2

Diag.5

Diag.6




M I C R O

S 6 0


Table 3

Barrillet

optionnel

(GBC217AS)

Optional

Tube holder

Marque

Trademark

Additif

Additive

Volume

Volume

Vide

Vacuum

Volume mort

(5)

Dead volume

Ref.

P/N

Modèle

Model

Photo N°

Photo #

Réglage

(1)

Adjustment

Perçage tube

Tube piercing

Bouchon

(2)

Stopper

POSITION 6

CONTROLE

R&D


sans bouchon

without stopper na

POSITION 5

STANDARD


with stopper

gomme

rubber


with stopper hemogard

Becton D. EDTA-K3 5ml 3ml 410µl 367856 Vacutainer 10 standard avec bouchonwith stopper

hemogard





Terumo EDTA-K2 5ml 3ml 430µl VP-053SDK Venoject II 15 standardavec bouchon

with stopper Ultraseal


with stopper

gomme

rubber


with stopper

gomme

rubber

ABX EDTA-K3 5ml 4ml 480µl ABX-3004002 nc 14 standardavec bouchon

with stopper







Greiner EDTA-K3 5ml 4ml 370µl 454036 Vacuette 13 standard

avec bouchon




POSITION 3 Becton D. nc 0.5ml na 30µl 365975 Microtainer 3avec tube

with tube

sans bouchon

without stopper

Microgard (Equipé adapteur:

autre réglage aiguille)

(Equipped with adaptor:

other adjustment)

POSITION 1 Becton D. nc 0.5ml na 30µl 365973 Microtainer 1 standardsans bouchon

without stopper na

OPTIONAL TUBE HOLDER

Groove Diag.8 Diag.9



M

I C

R O S 6

0 C



na - not applicable.

nc - not communicated.

(1) - The standard adjustment positions the needle at the bottom of the tube holder, when mentioned

"with tube", the presence of the tube is mandatory for the adjustment.

(2) - More information about sample tube is available in the user manual, section 3 "SPECIFICA-TIONS", point 3.4.2.

(3) - The thickness of the tube stopper blocks the tube into the piercing mechanism.

(4) - These volumes have been calculated; not measured.

(5) - The "dead volume" is determined after the manual adjustment of the sampling depth andincreased by 20%, except in the pediatric tubes where a 30µl volume has been fixed arbitrarily forsecurity.

2

1 3 4 5

6 7

8

9 10 11 12 13 14 1516

17

18

Diag.10

NOTE The needle heigth adjustments is explained in the procedure RAS 176 A.




M I C R O

S 6 0


2 - Maintenance

Diag.11

- Control the correct operation of the door lock electro magnet(diag 13).

3 - Description

Piercing blockleft hand side view

1 Door lock electro magnet

2 Door detection switch

$ 3 Sampling position switches

4 Gearings

Diag.12

Diag.13

1

2

3

4

Piercing blockright hand side view

Diag.14

Diag.15

- To clean the piercing block it is easier to dismantle the doorfront panel as shown on the diag 14.

- The sample tube holder can be pulled out from its locationas shown on diagram 15.- Re-install the reverse order.

CAUTION Decontamination procedure (RAS 192 A) must be followed to clean the sample tubeholder and its location.



RAA 009 A Ind.B Page 4/1 05/10/98

4 . OUTPUT FORMAT

CONTENTS 1. ARGOS FORMAT PRINCIPLES ............................................................................................................ 2

1.1. Introduction .................................................................................................................................... 21.2. Results characteristics ................................................................................................................... 2

1.2.1. Key ......................................................................................................................................... 21.2.2. Result format ......................................................................................................................... 31.2.3. End of communication key ..................................................................................................... 4

2. STANDARD FORMAT............................................................................................................................ 5

2.1 Message Structure .......................................................................................................................... 52.2. Details about the structure ............................................................................................................. 52.3. Identifier list and their formats ......................................................................................... ............... 5

2.3.1. Hematologyc numeric parameters ......................................................................................... 52.3.1.1. Format description .................................................................................................... 5 2.3.1.2. Identifier list ............................................................................................................... 6

2.3.2. Pathology ............................................................................................................................... 72.3.3. Histograms and matrix ........................................................................................................... 7

2.3.3.1. Format description .................................................................................................... 7 2.3.3.2. Identifier list ............................................................................................................... 7

2.3.4. Patient result identification ..................................................................................................... 82.3.4.1. Format description .................................................................................................... 8 2.3.4.2. Identifier list ............................................................................................................... 8

2.3.4.3. Analysis type ($80) ................................................................................................... 8 2.4. Heading title ................................................................................................................................... 82.5. Other identifiers .............................................................................................................................. 9

3. PIN ASSIGNMENTS ............................................................................................................................ 10



RAA 009 A Ind.B

4 . OUTPUT FORMAT

Page 4/2 05/10/98

3 RS ouput formats are available on MICROS 60 :

- Format 1 : ARGOS / HELIOS type- Format 2 : ABX internal format (factory use)- Standard format- TR off : Transmission off

1.1. Introduction

The ARGOS format is a fixed format (406 characters for one result) including a STX and aETX. These characters are splitted into fields representing a transmitted item.

STX ETX

406 CHARACTERS

DATA

STX ETX"R" / ANALYSER # / N°ID / ID / etc... CRC

1. ARGOS FORMAT PRINCIPLES

The fields have a fixed length separated by the OD character.

1.2. Results characteristics

1.2.1. Key

Total ASCII characters emitted : 406

- (-) : Space $20- (]) : Carriage return $0D

- CRC : exclusive "OR" of all the transmitted bytes except ETX and STX, then an inclusive"OR" with a $4O value.

- zzzzz : numeric field completed by zeros on the left.ex : 04.55 (decimal separation with a period).When the analyser does not transmit parameters, the field (zzzzz) is put in

place of (--.--).

- Y : Alphanumeric character from $20 to $7F.

- # : Space ($20) if automatic sampling. Star ($2A) if manual sampling.



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4 . OUTPUT FORMAT

1.2.2. Result format

Line 1 : STX ($02) Start of text 1

Line 2 : R ($52) Character "R" 1

Line 3 : zz] Analyser No 2 + 1

Line 4 : YYYYYYYYYYYYYYYY] Identification No 16 + 1

Line 5 : YYYYYYYYYYYYYYYYYYYYYYYYYYYYYY] Identification 30 + 1

Line 6 : zz/zz/zz-zzhzzmnzzs#] Time & Date 20 + 1

Line 7 : zzzzz-RN] GB rejection & limits 8 + 1

Line 8 : zzzzz-RN] LYC# rejection & limits 8 + 1

Line 9 : zzzzz-RN] LYC% rejection & limits 8 + 1

Line 10 : zzzzz-RN] MON# rejection & limits 8 + 1

Line 11 : zzzzz-RN] MON% rejection & limits 8 + 1

Line 12 : zzzzz-RN] GRA# rejection & limits 8 + 1

Line 13 : zzzzz-RN] GRA% rejection & limits 8 + 1

Line 14 : zzzzz-RN] NEU# rejection & limits 8 + 1

Line 15 : zzzzz-RN] NEU% rejection & limits 8 + 1Line 16 : zzzzz-RN] EOS# rejection & limits 8 + 1

Line 17 : zzzzz-RN] EOS% rejection & limits 8 + 1

Line 18 : zzzzz-RN] BAS# rejection & limits 8 + 1

Line 19 : zzzzz-RN] BAS% rejection & limits 8 + 1

Line 20 : zzzzz-RN] ALY# rejection & limits 8 + 1

Line 21 : zzzzz-RN] ALY% rejection & limits 8 + 1

Line 22 : zzzzz-RN] LIC# rejection & limits 8 + 1

Line 23 : zzzzz-RN] LIC% rejection & limits 8 + 1

Line 24 : zzzzz-RN] RET* rejection & limits 8 + 1




Line 28 : zzzzz-RN] RBC rejection & limits 8 + 1

Line 29 : zzzzz-RN] HGB rejection & limits 8 + 1

Line 30 : zzzzz-RN] HCT rejection & limits 8 + 1

Line 31 : zzzzz-RN] MCV rejection & limits 8 + 1

Line 32 : zzzzz-RN] MCH rejection & limits 8 + 1

Line 33 : zzzzz-RN] MCHC rejection & limits 8 + 1

Line 34 : zzzzz-RN] RDW rejection & limits 8 + 1


Line 36 : zzzzz-RN] PLT rejection & limits 8 + 1

Line 37 : zzzzz-RN] MPV rejection & limits 8 + 1

Line 38 : zzzzz-RN] PCT rejection & limits 8 + 1

Line 39 : zzzzz-RN] PDW rejection & limits 8 + 1

Line 40 : ABCDEFGHIJKLMNOPQRSTU] WBC 5 DIFF Flags 21 + 1

Line 41 : LMMGGG] WBC LMG Flags 6 + 1

Line 42 : PSM] PLT Flags 3 + 1

Line 43 : CRC 1

Line 44 : ETX ($03) end of text 1



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1.2.3. End of communication key

- (]) : Carriage return $0D

- CRC : the exclusive «OR» of all the transmitted bytes, exceptETX and STX, then the inclusive «OR» with a $40 value.

- zz : Number of the analyser.



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4 . OUTPUT FORMAT

2.1 Message Structure

STXSize + carriage return.

Identifier followed by heading title + carriage return.Identifier followed by the Information associated to the heading title + carriage return.Remainder of the other Identifiers and Informations associated to the heading title + carriagereturns.

Other heading titles + associated Informations................

Identifier followed by the Checksum + carriage returnETX

2.2. Details about the structure

Size : 5 bytes representing the total amount of the data except STX and ETX.Heading title : An 8 characters chain preceded by a space, indicating the associated data

type.Identifier : 1 byte (moving about $21 to $FF, it describes the information type which

follows this indicator).CheckSum : Sum modulo 65535 of all the characters except ETX, STX and all the infor

mations linked to the checksum (identifier - space - checksum) in thehexadecimal format on 4 bytes, preceded by a space.

2.3. Identifier list and their formats

2.3.1. Hematologyc numeric parameters

2.3.1.1. Format description

• Numerical field For all indicated parameters from $21 to $43, the format is a numerical field of 5 digits completed

with zeros on the left side (ex. : 04.55) and preceded by a space.The unit is the one chosen by the operator.When the parameter cannot be calculated by the analyzer, the field is replaced with (—.—).

• Parameter status Following the numerical field, a first digit gives the counting rejection status or the suspicion, asecond one gives the parameter value status according to high and low normalities, and to theoverloading capacities.

2. STANDARD FORMAT

The standard format can have a different a different number of fields according to thetransmitted items setup by the user (results, curves, flags, etc...).

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• Example 5.5 millions RBC with a counting error in the standard units :$32 $20 $30 $35 $2E $35 $30 $52 $68 $0D or «2 05.5Rh» + carriage return.The length is fixed and is worth 2+7+1, that is to say 10 bytes for one parameter.

2.3.1.2. Identifier list

First digit (letter) correspondance

R Parameter rejected for a counting default

S Suspicious parameter value

'space' No anomaly observed

Second digit ( letter) correspondance

l Parameter < to the low normal value

'space' Parameter normal value

h Parameter > to the high value

O Parameter exceeding the capacity

Identifiers Parameters Units

$21 ! WBC Standard - SI g/dl - SI mmoles

$22 " Lymphocytes (#)

$23 # (%)

$24 $ Monocytes (#)

$25 % (%)

$26 & Granulocytes (#)

$27 ' (%)


$32 2 RBC Standard - SI g/dl - SI mmoles

$33 3 HGB

$34 4 HCT

$35 5 MCV

$36 6 MCH

$37 7 MCHC

$38 8 RDW


$40 @ PLT Standard - SI g/dl - SI mmoles

$41 A MPV

$42 B THT

$43 C PDW



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4 . OUTPUT FORMAT

2.3.2. Pathology

* Flags associated with parameters

• Format description Flags are transmitted in a comprehensive mode, preceded by a space (same presentationthan on the screen, i.e. dependant from the language) 2 characters which are replacedwith spaces when the flag has not been detected.

• Identifier list

2.3.3. Histograms and matrix


• Histograms Histograms are transmitted on 128 or 256 channels, preceded by a space. They areautomaticaly rescaled to a 223 maximum amplitude value.

The zero amplitude value is $20, the maximum amplitude value is $FF.


Identifiers Parameters Formats Length

$50 P WBC or LMG L1M1M2G1G2G3 2 + 12 + 1

$53 S PLT PcScMc 2 + 6 + 1

Identifiers Parameters Formats Length

$57 W WBC Amplitude of each channel 2 + 128 + 1

$58 X RBC " "

$59 Y PLT " "

$5D ] WBC thresholds 5 thresholds 1 + 20 + 1

$5E ^ RBC thresholds 2 thresholds 1 + 8 + 1

$5F _ PLT thresholds 1 threshold 1 + 4 + 1



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2.3.4. Patient result identification


All the described fields have a fixed size character chain type and are completed with spacesfor the non significant informations.


2.3.4.3. Analysis type ($80) This identifier defines the analysis type CBC, LMG to carried out on the sample. It also providesthe analysis of one or several specific parameters.The CBC analysis includes the 12 parameters of the CBC's count.The LMG analysis includes the CBC analysis and the % and # of the 6 WBC populations.

2.4. Heading title

The heading title provides the data of the whole message : current hematological results orresults coming from statistics. This heading title is able to drive commands that can be interpretedby the analyser or by an external computer.

Analyzers being able to communicate in the bidirectionnal mode and supporting the remotecontrol mode, can interprete the heading title and runs the corresponding actions.This string is a 8 characters length, preceded by a space, containing data that follows, or thecommand type to be carried out. The identifier is $FF.

Identifiers Correspondance Formats Length

$70 p Analyzer number 01 2 + 2 + 1

$71 q Analysis date and time 94/06/06 13h15mn31s 2 + 19 + 1

$73 s Analyzer sequence number 0128 2 + 4 + 1

$74 t Sampling mode 'O' : open tube 'C' : close tube 2 + 1 + 1

$75 u Identification number 1450302154275-42 2 + 16 + 1

$76 v Identification SMITH Ronald 2 + 30 + 1

$80 ç Analysis type

Defined on 1 character (see

description)

'A' CBC analysis

'D' LMG analysis

From 'G' to 'Z' can be

configurated by the user

2 + 1 + 1

Identifier Correspondance Format Length

$FF type of data packet String of characters 2 + 8 + 1



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4 . OUTPUT FORMAT

Data exported by the analyzer

2.5. Other identifiers

Identifier $FC : This identifier allows the number transmission that could be an error #, aposition #, a burn-in sequence # or an hexadecimal status (see "Error list").Identifier $FD : Checksum value : see chapter on the message constitution.

Error list

Data packet string Use

RESULT

Hematological result transmission on a routine mode

Identifiers Correspondance Format Length

$FC Number On 8 bytes 2 + 8 + 1

$FD Checksum value hexadecimal on 4 Bytes 2 + 4 + 1

Error N° event linked to the analyzer

1 Operating temperature out of limits



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3. PIN ASSIGNMENTS



5. TRAINING SLIDES

Horiba ABX Micros 60 - Technical Manual

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