Honey Aloevera loaded electrospun PVA nanofibers as a potential...

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Transcript of Honey Aloevera loaded electrospun PVA nanofibers as a potential...

  • TEQIP SUMMER INTERNSHIP

    RESEARCH WORK REPORT

    ON

    Honey Aloevera loaded electrospun PVA nanofibers as a

    potential wound dressing material

    SUBMITTED TO:

    THE TEQIP OFFICE, IITH

    Under the guidance of

    Dr. Chandra Shekhar Sharma Associate Professor

    Creative & Advanced Research Based On Nanomaterials (CARBON) Laboratory

    Department of Chemical Engineering,

    Indian Institute of Technology, Hyderabad

    Submitted by:

    Apeksha Sharma

    B.E Chemical Engineering

    Madhav Institute of Technology and Science,Gwalior

  • CERTIFICATE

    This is to certify that the candidate Miss Apeksha Sharma, a 3rd year Bachelor of

    engineering student from the Department of Chemical Engineering, of Madhav

    Institute of Technology and Science, Gwalior has satisfactorily completed the

    Internship from 1st June 2018 to 30th June 2018 under my guidance. During her

    training period she has submitted a research work report entitled “Honey Aloevera

    loaded electrospun PVA nanofibers as a potential wound dressing material”

    which has been properly examined and found to be satisfactory.

    The candidate has fulfilled all the prescribed requirements.

    Dr. Chandra Shekhar Sharma Associate Professor

    Creative & Advanced Research Based On Nanomaterials (CARBON) Laboratory

    Department of Chemical Engineering,

    Indian Institute of Technology, Hyderabad

  • ACKNOWLEDGEMENT

    With great pleasure, I wish to put my deep gratitude and indebtedness to

    my respected summer internship guide, Dr. Chandra Shekhar Sharma, Associate Professor ,

    Dept. of Chemical Engineering , IIT Hyderabad for all the advice and valuable guidance that

    I received from him throughout this work.

    I am also grateful to Ms. Mrunalini Gaydhane, Research scholar, Dept. of Chemical

    Engineering , IIT (Hyderabad) who showed her great efforts and guidance at required times

    without which it would have been very difficult to carry out my research work. I am thankful

    to NPIU-MHRD TEQIP IIT Hyderabad which provided me such a great opportunity of

    internship.

    I would like to thank my friends and family members for providing me with the moral support in course of my program.

    - Apeksha Sharma

  • INDEX

    1. INTRODUCTION TO ELECTROSPINNING

    2. WOUND HEALING AND WOUND DRESSING

    3. MATERIAL SELECTION

    4. EXPERIMENTAL PROCEDURE

    a) Samples Preparation

    b) Characterization

     Optical Imaging

     FTIR (fourier transform infrared spectroscopy)

    5. RESULTS AND DISCUSSION

     Optical images

     FTIR plots

     Anti-oxidant Assay

    6.CONCLUSION

    7.REFERENCES

  • Electrospinning :

    Many techniques can be used to obtain nanofibers, such as drawing, template synthesis, phase

    separation and self-assembly, but electrospinning is the only process that can control fiber size,

    can be scaled for larger production, and is easily replicable. Electrospinning is a process that

    creates nanofibers through an electrically charged jet of polymer solution or polymer melt.

    Process description: The electrospinning process, in its simplest form consisted of a pipette to

    hold the polymer solution, two electrodes and a DC voltage supply in the kV range.The polymer

    drop from the tip of the pipette was drawn into a fiber due to the high voltage. The jet was

    electrically charged and the charge caused the fibers to bend in such a way that every time the

    polymer fiber looped, its diameter was reduced. The fiber was collected as a web of fibers on the

    surface of a grounded target [1].

    Schematic diagram of the electrospinning process

    Wound Healing and Wound Dressing

    Wound healing is a special biological process which is related to physiological parameters.

    Selection of a suitable wound dressing material for a specific type of wound requires

    comprehensive knowledge of the wound healing process [2].

    Phases of wound healing are:

  •  The first stage includes hemostasis and inflammation which occurs soon after there is

    damage to the skin.

     Another stage, In the migratory phase, the new and live cells called epithelial move towards

    skin injury to replace dead cells.

     The proliferation stage consists of the complete coverage of wound by epithelium.

     The final stage in the healing process of a wound is tissue remodeling.

    Wound Dressings

    Ordinary dressings, such as gauze, act as a common cover on a wound. The second types of

    wound dressings are interactive materials containing polymeric films or foams which are

    transparent and permeable to water vapor and atmospheric oxygen. These materials are good

    barriers against permeation of bacteria to the wound environment. The last types of wound

    dressings are bioactive materials or in other words active wound dressing materials (AWD)[3].

    Material selection:

    Polyvinyl alcohol (PVA)

    Poly(vinyl alcohol) is a water-soluble synthetic polymer. It has the idealized formula

    [CH2CH(OH)]n. It is used in papermaking, textiles, and a variety of coatings. It is white

    (colourless) and odorless. It is sometimes supplied as beads or as solutions in water.

    Biodegradable and non- toxic, high tensile strength [4].

    Aloevera: sometimes described as a "wonder plant," is a short-stemmed shrub. Aloe is a genus

    that contains more than 500 species of flowering succulent plants. Some uses: Soothes Rashes

    and Skin Irritations, Treats Burns and provides Antioxidants and Reduces Inflammation,Fast

    healing [5].

    Honey: Honey is a sweet liquid made by bees using the nectar from flowers. Honey also has

    antiseptic and antibacterial properties. Modern medical science has managed to find uses for

    honey in chronic wound management and combating infection [6].

    https://en.wikipedia.org/wiki/Soluble

  • EXPERIMENTAL PROCEDURE:

    a) Samples Preparation:

    1. PVA (polyvinyl alcohol) solution: [PVA].6 wt % of PVA salt in 10 ml water (solvent)

    2. PVA and aloevera solution:[PA].3ml PVA solution with 2ml aloevera solution

    3. PVA and honey solution :[PH].4ml PVA solution with 1ml honey

    4. PVA aloevera and honey solution:[PAH].3.5ml PVA with 1ml aloevera solution and 0.5 ml

    honey.Mats of fibers of above samples by electrospinning and also with some other parameters

    for optimization.

    b) Characterization

    1. Optical imaging: Optical microscopy is a technique employed to closely view a sample

    through the magnification of a lens with visible light. We get the optical images of nanofibers

    through optical microscope for optimization. Optical microscopy was done using Zeiss optical

    microscope.

    2. FTIR (fourier transform infrared spectroscopy)

    Fourier Transform-Infrared Spectroscopy (FTIR) is an analytical technique used to identify

    organic (and in some cases inorganic) materials. This technique measures the absorption of

    infrared radiation by the sample material versus wavelength

    FTIR was done by using Bruker Tensor-27 model.

    RESULTS AND DISCUSSION

     Optical images are as follows :- F=flowrate ,V=voltage

    Samples: 1. PA nanofibers (PVA+aloe)

  • [F=10ul,V=10KV,50x] [F=14ul,V=10KV,50x] (fused and beaded)

    2. PH nanofibers (PVA+honey)

    [F=10ul,V=12KV,50x] [F=14ul,V=10KV,50x] (wet fused)

    3.PAH nanofibers (PVA + Aloe +honey]

    [F=8ul, V=14KV,50x] [F=14ul,V= 14KV,50x]

    4.Plain PVA: [F=10ul,V=10KV]

  • FTIR PLOTS(wavenumber vs transmittance):

  • 0

    0.2

    0.4

    0.6

    0.8

    1

    1.2

    5001000150020002500300035004000

    PVA FTIR

    PVA Aloe vera Honey PH PA PAH

    Wave Number

    Analysis Wave number

    Analysis Wave Number

    Analysis Wave number

    Analysis Wave number

    Analysis Wave number

    Analysis

    3454 -OH (alcoholic)

    3341 Alcohol OH Stretch

    3292 Alcohol OH stretch

    3411 Alcohol OH stretch

    3439 Alcohol OH Stretch

    3428 Alcohol OH Stretch

    2926 -CH Alkyl

    stretch

    1638 C=C 2933 Carboxylic acid OH

    stretch

    2925 -CH Alkyl

    stretch

    2923 -C-H aldehydic

    2934 -C-H aldehydic

    2851 -CH Alkyl

    stretch

    552 C-Br 1644 C=C 1639 C=C 2853 -C-H aldehydic

    1733 C=O aldehyde

    2362 -CH 484 C-I 1418 N02 stretch

    1258 C-O-C stretch

    1642 C=C 1640 C=C

    1640 C=C 467 C-I 1028 C-F 1077 C-F 1384 C-F 1425 NO2 stretch

    1410 C=C 449 C-I 554 C-Br

    1260 C-F 1257 C-O-C stretch

    1253 C-OH 427 C-I 488 C-I

    1077 C-F

    1123 C-O 412 C-I 466 C-I