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Transcript of Hodgkin & Huxley II. Voltage Clamp MK Mathew NCBS, TIFR UAS – GKVK Campus Bangalore IBRO Course in...
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Hodgkin & Huxley II. Voltage Clamp
MK MathewNCBS, TIFRUAS – GKVK CampusBangalore IBRO Course in Neuroscience
Center for Cognitive Neuroscience & Semantics, University of LatviaRiga, LatviaAugust 21-August 29, 2013
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Voltage clamp of the squid axon. Vi is the internal potential measured with a pipette inserted in the axon. Ve is the external potential measured by an external electrode. Vm=Vi-Ve as computed by amplifier A1. A2 compares Vm with Vc (which is the command desired voltage) to inject current I, which maintains Vm at Vc. The current injected by the axial wire crosses the axonal membrane as it is drained by the chamber plates and measured by a current measuring device.
Bezanilla web page
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Nicholls “From Neuron to Brain”
Voltage Clamp currents in a Squid Axon. An axon is bathed in sea water and voltage clamped by the axial wire method. The membrane potential is held at -65 mV and then hyperpolarized in a step to -130 mV or depolarized in a step to 0 mV. Outward ionic current is shown as an upward deflection. The membrane permeability mechanisms are clearly asymmetrical. Hyperpolarization produces only a small inward current, whereas depolarization elicits a larger biphasic current. T = 3.8oC
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Nicholls “From Neuron to Brain”
IK = gK (Em – EK)
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Nicholls “From Neuron to Brain”
IK(t)= gK(t).(Em(t)– EK)
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Nicholls “From Neuron to Brain”
INa=gNa(Em – ENa)
2 terms: Rising phase and falling phase
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Reversal Potential
Steady State (Plateau) Value of IK
Peak INa
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Nicholls: Neuron to Brain
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V1/2
G/G
max
C O
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Fits to n4
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Fits to n4
Limiting value gK
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(Protein 3D Configurations)
K+ CHANNEL
Each of 4 gating particles can be either ON or OFF
Channel is open when all 4 Gating Particles are ON
Probability of the channel being OPEN is then n4
ON OFF
Putting n4 and the voltage dependence together:
Let the probability of a gating particle being ON be n
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The Potassium Channel
• The probability of a Gating Particle being ON:
• The probability of the channel being open:
• The conductance of a patch of membrane to K+ when all channels are open: (Constant obtained by experiments)
• The conductance of a patch of membrane to K+ when the probability of a subunit being open is n:
n4n
Kg
4 ngg Kk
extLLKKNaNam IVVgVVgVVgI )()()(
extLLKKNaNam IVVgVVngVVgI )()( )( 4
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Nicholls “From Neuron to Brain”
INa=gNa(Em – ENa)
2 terms: Rising phase and falling phase
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Na
Na+ CHANNEL
Na channel also has 4 sensors or gating particles
3 of these particles are involved in the CLOSED to OPEN transition m
1 of these particles is involved in INACTIVATION h
(Protein 3D Configurations)
Probability of C O gating particle being ON = mProbability of Channel being OPEN = m3
Probability of INACTIVATION particle being OFF = h
m
h
Probability of Channel being CONDUCTING = m3h
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The Sodium Channel (2)
• The probability of a fast subunit being open:
• The probability of a slow subunit being open:
• The probability of the channel being open:
• The conductance of a patch of membrane to Na+ when all channels are open: (Constant obtained by experiments)
• The conductance of a patch of membrane to Na+ :
m
hm3
Naghmgg NaNa
3
extLLKKNaNa IVVgVVgVVgdt
dVC )()()(
extLLKKNaNa IVVgVVngVVhmgdt
dVC )()( )( 43
h
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The Full Hodgkin-Huxley Model
extLLKKNaNa IVVgVVngVVhmgdt
dVC )()( )( 43
mm
m
mmmm mmm
dt
dm
,
1,
hh
h
hhhh hhh
dt
dh
,
1,
nn
n
nnnn nnn
dt
dn
,
1,
80
1.01
125.0)(
1
) 01.01.0()(
V
n
Vn
eV
e
VV
18
1.05.2
4)(
1
) 1.05.2()(
V
m
Vm
eV
e
VV
1
1)(
07.0)(
1.03
20
Vh
V
h
eV
eV
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C O
Resting Potential
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Depolarize theCell
Open SodiumChannels
-80 -60 -40 -20 0 20
0.0
0.2
0.4
0.6
0.8
1.0
Potential (mV)
Sod
ium
Con
du
ctan
ce
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V(x) = Vo e-x/l
rm/raxl
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m
m
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from Molecules of Life
The capacitance of the surface of a myelinated axon is about 1000 times smaller than that of an unmyelinated neuron
the conductance of the membrane to Na+ and K+ is also decreased, which has the effect of increasing the space constant for passive spread
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Myelination
Passive propagationRi: UnaffectedRm: Increased (series resistors)
Transient conductance is better:Cm: decreased (series capacitors
Metabolism is lower:No ion channels except at nodes
Amplification Saltatory conduction
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Nicholls “From Neuron to Brain”
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