HIV GP120 Envelope Protein

Objectives :

• Observing consensus sequences of different strains

• Structure analysis

GP120 FunctionGP120 involved in infection of human T-cells

HIV TYPES

HIV Type IHIV Type II

HIV Type III

SIV

Human viruses Simian virus

GP120 Sequence analysisSequence analysis shows a high mutation rate with preserved large consensus regions not only between strains, but also between different types

GP120 Sequence analysis

HIV Type I shows additional sequence of 7 aa in it’s consensus sequence in a consensus region in acids 27-33

consensus LWVTVFYGVPVWKEATTTLFCATDNK-------NTWATHACVPTDPDYQEVELNNVTENF 53

HIV1 LWVTVYYGVPVWHDADPVLFCASDAKahsteahNIWATQACVPTDPSPQEVFLPNVIESF 92

Two structures of GP120-CD4 from HIV 1 were analyzed

Hiv-1 Yu2 isolate Hiv-1 Hxbc2 isolate

Difference between the proteins

Score = 566 bits (1460), Expect = e-161Identities = 276/321 (85%), Positives = 289/321 (89%), Gaps = 8/321 (2%)

The similarity is shown, using protein blast

Structure of conserved region

GP120 Hxbc2 isolatePreserved region marked in yellow

CD4

Docking of GP120

Clustered solution ofDimers

With current approach GP120 exists as atrimers or tetramers on the membrane surface.According to J. Biol. Chem., Vol. 275, Issue 45, 35137-35145, November 10, 2000

Conclusions1. Preserved sequences found

2. GP120-CD4 theoretical docking performed, clustered solutions found

3. The results provide a basis for further research