“HIV-1 GENETIC VARIABILITY AND INTERSUBTYPE RECOMBINANT FORMS CIRCULATING IN THE NORTH OF...
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“HIV-1 GENETIC VARIABILITY AND INTERSUBTYPE RECOMBINANT FORMS CIRCULATING IN THE NORTH OF ANGOLA” may cause unprecedented challenges to diagnostic, treatment and prevention of HIV- 1. The aim of this work was to investigate the genetic diversity of HIV-1-positive Angolan samples. Angola: ~ 14 million inhabitants low HIV/AIDS prevalence levels (~ 2.1%, UNAIDS 2008). HIV-1 within the Angolan population shows very complex and high genotype diversity.
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“HIV-1 GENETIC VARIABILITY AND INTERSUBTYPE RECOMBINANT FORMS CIRCULATING IN THE NORTH OF ANGOLA”. Angola: ~ 14 million inhabitants. low HIV/AIDS prevalence levels (~ 2.1%, UNAIDS 2008 ). HIV-1 within the Angolan population shows very complex and high genotype diversity. - PowerPoint PPT Presentation
Transcript of “HIV-1 GENETIC VARIABILITY AND INTERSUBTYPE RECOMBINANT FORMS CIRCULATING IN THE NORTH OF...
“HIV-1 GENETIC VARIABILITY AND INTERSUBTYPE RECOMBINANT FORMS CIRCULATING IN THE NORTH OF ANGOLA”
may cause unprecedented challenges to diagnostic, treatment and prevention of HIV-1.
The aim of this work was to investigate the genetic diversityof HIV-1-positive Angolan samples.
Angola:
~ 14 million inhabitants
low HIV/AIDS prevalence levels (~ 2.1%, UNAIDS 2008).
HIV-1 within the Angolan population shows very complex and high genotype diversity.
METHODS and RESULTS:
A: 4% (n=4)
C: 15% (n=14)
D: 5% (n=5)
F1: 15% (n=14)
G: 6% (n=6)
H: 5% (n=5)K: 1% (n=1) U: 7%
(n=7)
CRFs: 21%
(n=20)
URF: 21% (n=20)
CRF02_AG: 25% (n=5)
CRF18_cpx: 20% (n=4)
CRF25_cpx: 5% (n=1)CRF45_cpx: 5% (n=1)
ANG_CRF: 45% (n=9)
High HIV-1 genetic diversity was depicted in the pol gene, with a predominance of intersubtype recombinant forms, CRFs and URFs (each with 21%), following subtypes C and F1 (each with 15%), U (untypable sequences, 7%), G (6%), D and H (each with 5%), A (4%) and K (each 1,04%) were found.
n = 96 HIV-1-positive Angolan patients were diagnosed and collected between 2008 and 2010 in Bengo.
Samples were genotyped based on phylogenetic and recombination analysis from pol (PR/RT) gene.
METHODS and RESULTS:
Nine out of twenty (45%) CRFs samples formed a unique cluster (U/H pattern) in the PR/RT region and were selected for DNA sequencing of the gag and partial env (gp41) gene regions.
. Further analysis of the gag and env regions indicated a CRF06_cpx gag/ H env gp41 pattern for all nine samples.
Gag (758 1905 bp): CRF06_cpx env (7638 8250 bp): H
ACKNOWLEDGEMENTS:
LABORATORY OF AIDS & MOLECULAR IMMUNOLOGY:- Drª. Mariza Morgado- Dr. Gonzalo Bello- Drª. Monick Guimarães
LABORATORY OF CLINICAL ANALYSIS:- Ms. Maria de Lurdes- Ms. Judith- Ms. Ludovina
PhD Scholarship
CENTRO DE SAÚDE SÃO LUCAS:- Drª. Martha Sojka- Patients
Molecular data have indicated a complex degree of HIV-1 group M genetic variability within the Angolan samples.
CONCLUSIONS:
Combined analysis of the nine sequences presenting the same recombinant profile in PR/RT- gag- env gp41 gene regions suggest a potential new HIV-1 CRF circulating in the North of Angola.
