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HISTOPATHOLOGY COURSE CONTENT

PART 1 - HISTOLOGY AND PATHOLOGY

1. Terminologies

2. Etiology of disease

3. Signs, symptoms and course of disease

4. Cellular and tissue changes

PART 2 - HISTOPATHOLOGIC TECHNIQUES AND PROCEDURES

  I. Introduction to Tissu Procssin!

1. Overvie

2. !ethods of tissue e"amination

3. #eceiving, custody and identification issues

4. $rossing and section cutting

  II. "i#$tion

1. %efinition

2. Characteristics of good fi"atives

3. Effects of fi"ation

4. !ethods of &i"ation

'. Types of Chemical fi"atives(. &actors affecting the rate of fi"ation

). Secondary fi"ation and post*chromati+ation

  III. Dc$%ci&ic$tion

1. %efinition

2. %ecalcifying agents

3. !ethods of decalcification

4. &actors affecting the process of decalcification

'. ays of measuring the e"tent of decalcification

(. Tissue softeners

  I'. D()dr$tion

1. %efinition

2. Characteristics3. !ethods of dehydration

4. Types of dehydrating agents

'. -dvantages and disadvantages

  '. C%$rin!*D$%co(o%i+$tion

1. %efinition

2. Characteristics

3. Clearing agents

4. Composition and preparation

'. End*point of clearing

  'I. I,r!n$tion*In&i%tr$tion

1. %efinition

2. Types of impregnation

  'II. E,ddin!/ C$stin!/ 0%ocin! or o%din!.

1. %efinition

2. Types

3. Orientation

4. Trimming

  'III. icroto,/ Sction Cuttin! $nd Ad(si3s

1. Types

2. Care

3. Techniues

4. /arts

'. 0oning(. Stropping

). /rolems and resolutions encountered in Section Cutting

. -dhesives

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  I4. St$inin!

1. %efinition

2. Types

3. Composition and

4. !ethods

'. mmuno*stains

(. Special stains  4. ountin!

1. Types

2. Composition and preparation

  4I. L$%in!

1. Techniues

2. /recautions

  4II. C%% 0%ocin!

1. %efinition

2. Specimens

3. &i"ation

4. /rocedures and techniues

  4III. Auto,$td Tissu Procssin!1. %efinition

2. Types

3. -dvantages and %isadvantages

  4'I. 0ios)

1. E"cision

2. ncision

3. Core*needle iopsy

4. &ine needle aspiration iopsy

'. /rocessing

PART 5 - CYTOLOGICAL TECHNIQUES AND PROCEDURES

1. %efinition2. mportance

3. Specimens

4. &i"ation

'. Staining Techniues

(. !ethods of #eporting

). nterpretation of #esults Clinical Significance5

PART 6 - AUTOPSY * POST ORTE E4AINATION 7PE8

1. Signs of somatic death /ost mortem changes5

2. /urpose of /!E

3. Techniues of /!E

4. #euirements of /!E

'. /ersonnel involved in /!E

(. !edical autopsy vs. medico*legal autopsy

). Classification of autopsy specimens.

. /roper handling of autopsy specimen.

P$rt 9 - T L$:s/ R%$td L$:s/ $nd Cod o& Et(ics

1. !T 6as

2. 6aoratory !anagement

3. #elated 6as

4. Code of Ethics including 7ioethics

Sc(du% o& Qui++s89: 1 * /art 1 to /art 2;<

  89: 2 * /art 2;< to /art 2;=

  89: 3 * /art 2;= to /art 2; =<

  89: 4 * /art 3 to /art '

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