Histology Lab Slides
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Transcript of Histology Lab Slides
Epithelial Tissue
• Characteristics:
Simple Cuboidal
• Description: Single layer of cube-shaped cells.
• Location: small ducts of glands, kidney tubules, surface of ovary.
• Function: secretion & absorption
Cell is as wide as it is high. Onecentral nucleus
(= single layer)
Simple Cuboidal
• Cells closely packed together and touching one another
• Has a basal surface and apical surface
• Avascular
• Sits on a basement membrane (difficult to see on slide)
Lumen:Inside space of Tubular structure
Nucleus ApicalSurface
BasalSurface
Non- Ciliated Simple Columnar• Description: Single
layer of non-ciliated rectangular cells; contain goblet cells and sometimes microvilli.
• Location: GI tract, ducts, gallbladder
• Function: secretion and absorption
Goblet Cell
Nucleus
Goblet Cell
• Nuclei lie near base of cell• Goblets secrete mucous at
apical surface to lubricate lining of GI, respiratory, reproductive and urinary systems.
• Microvilli increase surface area for absorption.
Simple Columnar Cells
Pseudostratified Ciliated Columnar Epithelium
• Appears stratified because nuclei are at varying levels.
• Ciliated lines airways of upper respiratory tract.
• Mucus from Goblets traps foreign particles and are swept away by cilia.
Goblet Cells
Cilia
Nuclei at varying levels
Goblet Cells secreting mucus
Epithelial and Connective
• 1 – pseudostratified columnar epithelial
• 2- simple cuboidal epithelial
• 3- hyaline cartilage connective tissue
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2
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Fibroblast CELLS
MATRIXFIBER
Elastic Fiber
Collagen Fiber (thicker pink ones)
GROUND SUBSTANCELoose Areolar
Connective Tissue
Teased Dense Regular Connective Tissue
• Forms tendons and ligaments and aponeuroses.
• Matrix looks white and shiny.
• Mostly collagen fibers with fibroblasts between rows.
• Strong attachment!
Teased Dense Regular CT (magnified)
Fibers are mostlyCollagen.
Fibroblasts
Reticular Connective Tissue
• Network of interlacing reticular fibers & reticular cells
• Found in stroma (framework) of liver, spleen, lymph nodes, red bone marrow
• Acts as a filter in spleen and lymph nodes.
Blue stainingReticular fibers
Reticular Cells
Reticular Connective Tissue
• Remember that connective tissue is vascular. (Avascular epithelial tissue depends on diffusion of nutrients and waste through blood vessels in CT.
Blood vessel:Single layer of Epithelial cells forms vessel tube.
ReticularCT
• 1- Reticular cells
• 2- Blood vessel
• 3- Reticular fibers
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Hyaline Cartilage
• Often called gristle• Bluish white smokey
looking ground substance • Numerous chondrocytes• Location: ends of long
bones, ribs, nose, trachea, larynx, bronchi
• For flexibility and support• Most abundant type of
cartilage in body
chondrocytes
Ground Substance
Elastic Cartilage
• Chondrocytes are located within threadlike network of elastic fibers
• Location: Epiglottis of larynx, external ear, Eustachian tubes
• For support and shape
chondrocyte
Lacuna(hollow spaceholding chondrocyte)
Elastic vs Hyaline Cartilage
Elastic fibers throughoutGround substance
SmootherAppearanceOf groundsubstance
AdiposeTissue
• Adipocytes, “signet ring” cells with peripheral nuclei.
• For storage of triglycerides, to reduce heat loss, and serve as an energy reserve.
• Location: under skin, around heart & kidneys, yellow marrow, behind eyeball
“signet ring” adipocyte
nucleus
Osseous Tissue (Bone)• Compact bone consists of
osteons (haversion systems) that contain:– Lamellae– Lacuna– Osteocytes– Canaliculi– Central (haversion)canals
• Spongy bone consists of thin plates called trabeculae filled with red marrow
HaversionSystem
Lamellae
Central(Haversion)Canal
Lacunae
Lacunae (where osteocytes reside)
Canaliculi: small Tunnels where osteocyteprocesses run for diffusionof nutrients and wastes
Skeletal Muscle Tissue
• Striated– Proteins
• Actin• Myosin
• Multinucleated• Voluntary• Attach to bones• For movement,
communication, posture, heat production
Striations
Skeletal muscle fiber with multiple nuclei
Smooth (Visceral) Muscle Tissue• No striations• Spindle-shaped• Single centrally located
nucleus• Nucleus is long and cigar
shaped• Involuntary• Viscera• Walls of hollow structures
– Blood vessels– Airways to lungs– Stomach– Intestines– Gallbladder– Urinary bladder
Cigar shaped nuclei
Smooth Muscle
Cardiac Muscle Tissue
• Striated, mono-nucleated, branching
• Self excitatory• Intercalated discs
attach fibers together and contain gap junctions (help with conduction of electrical signals)
Intercalated disc
nucleus
Name the tissues and structures you see.
Simple Squamous Epithelial
Stratified Squamous Epithelial
These keratinized epithelial cells willBe sloughed off.
Basal Layer
Apical Layer